International Journal of Homeopathy & Natural Medicines

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In silico Characterization and Selection of Epitope-based Peptide Vaccines Against Ebola Viruses

Received: 20 February 2020    Accepted: 03 March 2020    Published: 24 March 2020
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Abstract

Background & Objective: To analyze the mutual B and T cell epitope related vaccine which can evoke the immune response against Ebola hemorrhagic fever. Ebola virus is pathogenic in nature which is associated with a systemic disease in man and apes. Ebola virus disease is advised to be zoonotic with random spillovers to human beings, particularly animals, and apes. These viruses can affect on immune suppression, abnormal inflammatory responses and high mortality. Methodology: In this study, membrane proteins NP, VP35, VP40, sGP, ssGP, VP30, and VP24 of Ebola virus were retrieved from the protein databases and subjected to many bioinformatics related tools to identify the antigenic B and T-cell epitopes using antigenicity analysis. The selected epitopes were subjected to molecular docking simulation along with HLA-DR to affirm their antigenicity in silico. Result & Conclusion: The data present in our study exposed that the epitopes from NP, VP35, VP40, sGP, ssGP, VP30, and VP24 proteins might be the specific target for Ebola virus based on the best binding affinity and molecular docking score. The biochemical analysis and various characterization is also mandatory to evaluate the correlation of epitopes solely with the MHC molecules.

DOI 10.11648/j.ijhnm.20200601.11
Published in International Journal of Homeopathy & Natural Medicines (Volume 6, Issue 1, June 2020)
Page(s) 1-5
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Ebola Virus, Peptide Vaccine, TMHMM, BCpreds, Propred, AutoDock

References
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Author Information
  • Department of Biotechnology, Indian Academy Degree College, Bangalore, India

  • Department of Biotechnology, Indian Academy Degree College, Bangalore, India

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  • APA Style

    Sameer Sharma, Sudhakar Malla. (2020). In silico Characterization and Selection of Epitope-based Peptide Vaccines Against Ebola Viruses. International Journal of Homeopathy & Natural Medicines, 6(1), 1-5. https://doi.org/10.11648/j.ijhnm.20200601.11

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    ACS Style

    Sameer Sharma; Sudhakar Malla. In silico Characterization and Selection of Epitope-based Peptide Vaccines Against Ebola Viruses. Int. J. Homeopathy Nat. Med. 2020, 6(1), 1-5. doi: 10.11648/j.ijhnm.20200601.11

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    AMA Style

    Sameer Sharma, Sudhakar Malla. In silico Characterization and Selection of Epitope-based Peptide Vaccines Against Ebola Viruses. Int J Homeopathy Nat Med. 2020;6(1):1-5. doi: 10.11648/j.ijhnm.20200601.11

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  • @article{10.11648/j.ijhnm.20200601.11,
      author = {Sameer Sharma and Sudhakar Malla},
      title = {In silico Characterization and Selection of Epitope-based Peptide Vaccines Against Ebola Viruses},
      journal = {International Journal of Homeopathy & Natural Medicines},
      volume = {6},
      number = {1},
      pages = {1-5},
      doi = {10.11648/j.ijhnm.20200601.11},
      url = {https://doi.org/10.11648/j.ijhnm.20200601.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijhnm.20200601.11},
      abstract = {Background & Objective: To analyze the mutual B and T cell epitope related vaccine which can evoke the immune response against Ebola hemorrhagic fever. Ebola virus is pathogenic in nature which is associated with a systemic disease in man and apes. Ebola virus disease is advised to be zoonotic with random spillovers to human beings, particularly animals, and apes. These viruses can affect on immune suppression, abnormal inflammatory responses and high mortality. Methodology: In this study, membrane proteins NP, VP35, VP40, sGP, ssGP, VP30, and VP24 of Ebola virus were retrieved from the protein databases and subjected to many bioinformatics related tools to identify the antigenic B and T-cell epitopes using antigenicity analysis. The selected epitopes were subjected to molecular docking simulation along with HLA-DR to affirm their antigenicity in silico. Result & Conclusion: The data present in our study exposed that the epitopes from NP, VP35, VP40, sGP, ssGP, VP30, and VP24 proteins might be the specific target for Ebola virus based on the best binding affinity and molecular docking score. The biochemical analysis and various characterization is also mandatory to evaluate the correlation of epitopes solely with the MHC molecules.},
     year = {2020}
    }
    

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    T1  - In silico Characterization and Selection of Epitope-based Peptide Vaccines Against Ebola Viruses
    AU  - Sameer Sharma
    AU  - Sudhakar Malla
    Y1  - 2020/03/24
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    N1  - https://doi.org/10.11648/j.ijhnm.20200601.11
    DO  - 10.11648/j.ijhnm.20200601.11
    T2  - International Journal of Homeopathy & Natural Medicines
    JF  - International Journal of Homeopathy & Natural Medicines
    JO  - International Journal of Homeopathy & Natural Medicines
    SP  - 1
    EP  - 5
    PB  - Science Publishing Group
    SN  - 2472-2316
    UR  - https://doi.org/10.11648/j.ijhnm.20200601.11
    AB  - Background & Objective: To analyze the mutual B and T cell epitope related vaccine which can evoke the immune response against Ebola hemorrhagic fever. Ebola virus is pathogenic in nature which is associated with a systemic disease in man and apes. Ebola virus disease is advised to be zoonotic with random spillovers to human beings, particularly animals, and apes. These viruses can affect on immune suppression, abnormal inflammatory responses and high mortality. Methodology: In this study, membrane proteins NP, VP35, VP40, sGP, ssGP, VP30, and VP24 of Ebola virus were retrieved from the protein databases and subjected to many bioinformatics related tools to identify the antigenic B and T-cell epitopes using antigenicity analysis. The selected epitopes were subjected to molecular docking simulation along with HLA-DR to affirm their antigenicity in silico. Result & Conclusion: The data present in our study exposed that the epitopes from NP, VP35, VP40, sGP, ssGP, VP30, and VP24 proteins might be the specific target for Ebola virus based on the best binding affinity and molecular docking score. The biochemical analysis and various characterization is also mandatory to evaluate the correlation of epitopes solely with the MHC molecules.
    VL  - 6
    IS  - 1
    ER  - 

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