| Peer-Reviewed

Effect of Milk Protein Hydrolysate on Some Hematological Parameters of Type II Diabetic Rats

Received: 8 March 2019     Accepted: 13 April 2019     Published: 20 May 2019
Views:       Downloads:
Abstract

Milk protein-derived bioactive peptides could be used in human nutrition in both newborns and adults, since they are claimed to be health-improving components that can be used to reduce the risk of disease or to enhance certain physiological functions. This study investigated the effect of oral intake of milk protein concentrate (MPC) and milk protein concentrate hydrolysate (MPCH) on body weight, organ relative weight, and hematological parameters in alloxan-induced diabetes rats. The animals were divided into six major groups after stabilization of diabetes for one week. Diabetic and non-diabetic rats groups were received intra-gastric dose of MPC or MPCH (150mg/kg B.W) for six weeks. Feed treatment by MPC and MPCH significantly (P<0.05) increased the final body weight of normal rats and improved the body weight of diabetic rats and significantly protective (P<0.05) the liver, kidney, heart and lung of normal rats and reduced the fallers of these organs in diabetic rats. The diabetes groups treated by MPCH showed improvement in RBC, Hb, Ht and PLT values. Meanwhile WBC not affected. The oral intake of MPCH protected the normal range of Lymphocytes and S. Nutrophils in healthy and diabetic rats. The oral intake of MPCH caused significant (P<0.05) decrease of Monocytes in diabetic and non-diabetic rats, while the oral intake of MPC had no affected on Monocytes of diabetic and non-diabetic rats. MPC and MPCH reduced the harmful effect of T2D on body weight, organs weights of rats, and improvement most hematological parameters of normal and diabetes rats.

Published in International Journal of Homeopathy & Natural Medicines (Volume 5, Issue 1)
DOI 10.11648/j.ijhnm.20190501.14
Page(s) 18-29
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Milk Protein, Protein Hydrolysate, Bioactive Peptides, T2 Diabetes

References
[1] Harlev E, E. Nevo, N. Mirsky and R. Ofir, 2013. Antidiabetic attributes of desert and steppic plants: a review. Planta Medica, 79: 425-436.
[2] Mirmiran P, Z. Bahadoran, F. Azizi, 2014. Functional foods-based diet as a novel dietary approach for management of type 2 diabetes and its complications: A review. World Journal of Diabetes, 5: 267- 281.
[3] Schrezenmeir, J. and A. Jagla, 2000. Milk and diabetes. Journal of the American College of Nutrition, Vol. 19, No. 2: 176S–190S.
[4] WHO, 2006. Definition and diagnosis of diabetes mellitus and intermediate hyperglycemia: report of a WHO/IDF consultation.
[5] Tamrakara, A. K., N. Jaiswala., P. P. Yadavb., R. Mauryab. and A.K. Srivastavaa, 2011. Pongamol from Pongamiapinnata stimulates glucose uptake by increasing surface GLUT4 level in skeletal muscle. Molecular and Cellular Endocrinology, 339: 98–104.
[6] Hirahatake K. M, J. L. Slavin, K. C. Maki and S. H. Adams, 2014. Associations between dairy foods, diabetes, and metabolic health: potential mechanisms and future directions. Metabolism, 63: 618-627.
[7] Sun, X and M.B. Zemel. 2006. Dietary calcium regulates ROS production in aP2-agouti transgenic mice on high-fat/ high-sucrose diets. International Journal of Obesity, 230: 1341-1346.
[8] Jakubowicz, D and O. Froy, 2013. Biochemical and metabolic mechanisms by which dietary whey protein may combat obesity and Type 2 diabetes. The Journal of Nutritional Biochemistry, 24: 1-5.
[9] Yan, J., Zhao, J., Yang R and Zhao, W, 2019. Bioactive peptides with antidiabetic properties: a review. International Journal of Food Science and Technology. doi:10.1111/ijfs.14090
[10] Awad, S, M.I. El-Sayed, A. Wahba, A. El Attar, M. I. Yousef and M. Zedan, (2016). Antioxidant activity of milk protein hydrolyaste in alloxan-induced diabetic rats. Journal of Dairy Science, 99: 8499–8510.
[11] Korhonen, H., and A. Pihlanto, 2003. Food-derived bioactive peptides opportunities for designing future foods. Current Pharmaceutical Design, 9: 1297–1308.
[12] Hartmann, R., H. Meisel, 2007. Food-derived peptides with biological activity: from research to food applications. Curr. Opin. Biotechnol, 18: 163–169.
[13] Hajirostamloo, B, 2010. Bioactive component in milk and dairy product. World academy of science, Engineering and Technology, 72: 162-166.
[14] Plaisanciéa, P., J. Claustrec., M. Estiennea., G. Henryd., R. Boutroud., A. Paqueta and J. Léonild, 2013. A novel bioactive peptide from yoghurts modulates expression of the gel-forming MUC2 mucin as well as population of goblet cells and Paneth cells along the small intestin. Journal of Nutritional Biochemistry, 24: 213–221.
[15] Marcone S., Belton, O & Fitzgerald D. J, 2017. Milk-derived bioactive peptides and their health promoting effects: a potential role in atherosclerosis. British Journal of Clinical Pharmacology, 83: 152–162.
[16] Kamau, S. M., R.R. Lu., W. Chen, X.M. Liu., F. W. Tian., Y.Shen and T. Gao, 2010. Functional significance of bioactive peptides derived from milk proteins. Food Reviews International, 26: 386–401.
[17] Guilloteau, P., V. Rome´., L. Delaby., F. Mendy., L. Roger and J. A. Chayvialle, 2009. A new role of phosphopeptides as bioactive peptides released during milk casein digestion in the young mammal: Regulation of gastric secretion. Peptides; 30: 2221–2227.
[18] Pan, D. D., Z. Wu., J. Liu., X. Y. Cao and X. Q. Zeng, 2013. Immunomodulatory and hypoallergenic properties of milk protein hydrolysates in ICR mice. Journal of Dairy Science., 96: 4958–4964.
[19] Mansour, H. A., A. S. Newairy., M. I. Yousef and S. A. Sheweita, 2002. Biochemical study on the effects of some Egyptian herbs in alloxan-induced diabetic rats. Toxicology, 170 (3): 221-228.
[20] Sheweita, S. A., A. A. Newairy., H. A. Mansour and M. I. Yousef, 2002. Effect of some hypoglycemic herbs on the activity of phase I and II drug-metabolizing enzymes in alloxan-induced diabetic rats. Toxicology, 174: 131–139.
[21] Otte, J., S. M. Shalaby., M. Zakora., A. H. Pripp and S. A. El-Shabrawy, 2007. Angiotensin-converting enzyme inhibitory activity of milk protein hydrolysates: Effect of substrate, enzyme and time of hydrolysis. International Dairy Journal, 17: 488–503.
[22] El-Sayed, M. I, S. Awad., A.Wahba., A. El Attar., M.I. Yousef and M. Zedan, 2016. In Vivo Anti-diabetic and Biological Activities of Milk Protein and Milk Protein Hydrolyaste. Advances in.Dairy,Research, 1000154.
[23] Laemmli, U. K, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 277: 680-685.
[24] Hames, B. D. and D. Rickwood, 1990. In: Gel electrophoresis of proteins. A practical approach. TRL, London, England. Puplishing Co.pp.34, 36, 37, 44, 45 and 48.
[25] Awad, S., Q. Q. Lǘthi-Peng and Z. Puhan, 1998. Proteolytic activities of chymosin and porcine pepsin on buffalo, cow, and goat whole and β-Casein fractions. Journal of Agriculture and Food Chemistry, 46: 4997-5007.
[26] AOAC. (2005) Official Methods of Analysis of AOAC International. (18th ed.) Gaithersburg.
[27] Childs, A. C., S. L. Phaneuf., A. J. Dirks., T. Phillips and C. Leeuwenburgh, 2002. Doxorubicin treatment in vivo causes cytochrome C release and cardiomyocyte apoptosis, as well as increased mitochondrial efficiency, superoxide dismutase activity, and Bcl-2: Bax ratio. Cancer Res; 62: 4592-4598.
[28] Zhang, H., Wang, J., Liu, Y& Sun B, (2015). Peptides Derived from Oats Improve Insulin Sensitivity and Lower Blood Glucose in Streptozotocin-Induced Diabetic Mice. Journal of Biomedical Sciences, 4: 1-7.
[29] SAS (2004).SAS Procedure Guide "Version 6.12 Ed." SAS Institute Inc., Cary.
[30] Helal, E. G. E., A. S. M. Gawish and A. Kahwash, 2005. Some hematological- studice on diabetic rats - treated with certain hypoglycemic plants. The Egyptian Journal of Hospital Medicine, 19: 179 – 188.
[31] Mahmoud, A.M, 2013. Hematological alteration in diabetic rats-role of adipocytokines and effect of citrus flavonoids. EXCLI Journal, 12: 647- 657.
[32] Weiss, G. and L. T. Goodnough, 2005. Anemia of chronic disease. N Engl J Med; 352: 1011–23.
[33] Oyedemi, S. O., M. T. Yakubu and A. J. Afolayan, 2011. Antidiabetic activities of aqueous leaves extract of Leonotis leonurus in streptozotocin induced diabetic rats. J. Med. Plant Res., 5: 119-125.
[34] Arun, G. S. and K. G. Ramesh, 2002. Improvement of insulin sensitivity by perindopril in spontaneously hypertensive and streptozotocindiabetic rats. Indian J Pharmacol, 34: 156- 64.
[35] Kang-xin., F., Yunzhong., Xin-Wanjuan and P.U. Sunchunr, (1990). Observation on the effect of irradiation in vitro and in vivo on SH- group of rat erythrocyte membrane by spine label technique. J. Radia. Res. and Radia process, 8 (2): 103-112.
[36] Helal, E. G. E, 2000. Effectiveness of an herbal mixture with treatment of noninsulin dependent diabetes mellitus. Al-Azhar Bull. Sc., 1: 201- 324.
[37] Ganong, W. F, 2003. Review of medical physiology 23rd ed., Lange med. Public, 19: 306-326.
Cite This Article
  • APA Style

    Mahmoud Ibrahim El-Sayed, Sameh Awad, Mokhtar Yousef, Abdelmonem Wahba, Aisha El Attar, et al. (2019). Effect of Milk Protein Hydrolysate on Some Hematological Parameters of Type II Diabetic Rats. International Journal of Homeopathy & Natural Medicines, 5(1), 18-29. https://doi.org/10.11648/j.ijhnm.20190501.14

    Copy | Download

    ACS Style

    Mahmoud Ibrahim El-Sayed; Sameh Awad; Mokhtar Yousef; Abdelmonem Wahba; Aisha El Attar, et al. Effect of Milk Protein Hydrolysate on Some Hematological Parameters of Type II Diabetic Rats. Int. J. Homeopathy Nat. Med. 2019, 5(1), 18-29. doi: 10.11648/j.ijhnm.20190501.14

    Copy | Download

    AMA Style

    Mahmoud Ibrahim El-Sayed, Sameh Awad, Mokhtar Yousef, Abdelmonem Wahba, Aisha El Attar, et al. Effect of Milk Protein Hydrolysate on Some Hematological Parameters of Type II Diabetic Rats. Int J Homeopathy Nat Med. 2019;5(1):18-29. doi: 10.11648/j.ijhnm.20190501.14

    Copy | Download

  • @article{10.11648/j.ijhnm.20190501.14,
      author = {Mahmoud Ibrahim El-Sayed and Sameh Awad and Mokhtar Yousef and Abdelmonem Wahba and Aisha El Attar and Mostafa Zedan},
      title = {Effect of Milk Protein Hydrolysate on Some Hematological Parameters of Type II Diabetic Rats},
      journal = {International Journal of Homeopathy & Natural Medicines},
      volume = {5},
      number = {1},
      pages = {18-29},
      doi = {10.11648/j.ijhnm.20190501.14},
      url = {https://doi.org/10.11648/j.ijhnm.20190501.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijhnm.20190501.14},
      abstract = {Milk protein-derived bioactive peptides could be used in human nutrition in both newborns and adults, since they are claimed to be health-improving components that can be used to reduce the risk of disease or to enhance certain physiological functions. This study investigated the effect of oral intake of milk protein concentrate (MPC) and milk protein concentrate hydrolysate (MPCH) on body weight, organ relative weight, and hematological parameters in alloxan-induced diabetes rats. The animals were divided into six major groups after stabilization of diabetes for one week. Diabetic and non-diabetic rats groups were received intra-gastric dose of MPC or MPCH (150mg/kg B.W) for six weeks. Feed treatment by MPC and MPCH significantly (PPP<0.05) decrease of Monocytes in diabetic and non-diabetic rats, while the oral intake of MPC had no affected on Monocytes of diabetic and non-diabetic rats. MPC and MPCH reduced the harmful effect of T2D on body weight, organs weights of rats, and improvement most hematological parameters of normal and diabetes rats.},
     year = {2019}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Effect of Milk Protein Hydrolysate on Some Hematological Parameters of Type II Diabetic Rats
    AU  - Mahmoud Ibrahim El-Sayed
    AU  - Sameh Awad
    AU  - Mokhtar Yousef
    AU  - Abdelmonem Wahba
    AU  - Aisha El Attar
    AU  - Mostafa Zedan
    Y1  - 2019/05/20
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ijhnm.20190501.14
    DO  - 10.11648/j.ijhnm.20190501.14
    T2  - International Journal of Homeopathy & Natural Medicines
    JF  - International Journal of Homeopathy & Natural Medicines
    JO  - International Journal of Homeopathy & Natural Medicines
    SP  - 18
    EP  - 29
    PB  - Science Publishing Group
    SN  - 2472-2316
    UR  - https://doi.org/10.11648/j.ijhnm.20190501.14
    AB  - Milk protein-derived bioactive peptides could be used in human nutrition in both newborns and adults, since they are claimed to be health-improving components that can be used to reduce the risk of disease or to enhance certain physiological functions. This study investigated the effect of oral intake of milk protein concentrate (MPC) and milk protein concentrate hydrolysate (MPCH) on body weight, organ relative weight, and hematological parameters in alloxan-induced diabetes rats. The animals were divided into six major groups after stabilization of diabetes for one week. Diabetic and non-diabetic rats groups were received intra-gastric dose of MPC or MPCH (150mg/kg B.W) for six weeks. Feed treatment by MPC and MPCH significantly (PPP<0.05) decrease of Monocytes in diabetic and non-diabetic rats, while the oral intake of MPC had no affected on Monocytes of diabetic and non-diabetic rats. MPC and MPCH reduced the harmful effect of T2D on body weight, organs weights of rats, and improvement most hematological parameters of normal and diabetes rats.
    VL  - 5
    IS  - 1
    ER  - 

    Copy | Download

Author Information
  • Department of Dairy Technology Research, Food Technology Research Institute, ARC, Giza, Egypt

  • Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University, Alexandria, Egypt

  • Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt

  • Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University, Alexandria, Egypt

  • Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University, Alexandria, Egypt

  • Department of Dairy Technology Research, Food Technology Research Institute, ARC, Giza, Egypt

  • Sections