International Journal of Animal Science and Technology

| Peer-Reviewed |

Effects of GnRH on Conception Rate at the Time of Artificial Insemination in Crossbred Dairy Cows

Received: 20 November 2017    Accepted: 07 December 2017    Published: 03 January 2018
Views:       Downloads:

Share This Article

Abstract

The present study was conducted to investigate the influence of GnRH (Gonadotropin Releasing Hormone) administration on conception rate at the time of artificial insemination (AI) in healthy crossbred dairy cows. A total 90 cows were recruited and a preset questionnaire was answered by the owners. The cows were divided randomly in to two equal groups. GnRH treated group was injected intramuscularly (i/m) in the neck muscle with GnRH (Gonadorelin Acetate) at the time of AI. Again the treated group was divided randomly into three equal groups (15 cows each) for three different doses of GnRH (200, 250, 300 µg). The necessary information was taken from the owner with pretested questionnaire. The overall 71.11% conception rate was found in GnRH treated group on the other hands 55.56% conception rate was in control groups and having significant effect (P<0.05). There were significant effect (P<0.05) of age and milk yield and there were no significant effect of doses, timing, body condition score and parity within GnRH treated group. The study revealed that use of GnRH improved the conception rate in crossbred dairy cows at the time of AI.

DOI 10.11648/j.ijast.20170101.14
Published in International Journal of Animal Science and Technology (Volume 1, Issue 1, December 2017)
Page(s) 19-34
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Conception Rate, Crossbred Dairy Cows, GnRH and Artificial Insemination

References
[1] Alam J 1991. Livestock development strategies in Bangladesh. The Socioeconomic Perspective. Proceeding of workshop on Livestock Development in Bangladesh. BLRI, Savar, Dhaka.
[2] Alam MGS and Gosh A 1994. Reproductive performance in cows: it’s relationship to parity and season. Bangladesh Veterinary Journal 12:51-61.
[3] Alam MGS and Rahman A 1979. Diseases of the genital tract of indigenous cows in Bangladesh. Tropical Animal Health Production 11: 179-180.
[4] Anjum IA 2010. Improvement of Conception R in Crossbred Cattle by Using GnRH analogue Therapy. Agriculturatropicaetsubtropica vol. 43.
[5] BBS 2001. Bangladesh Bureau of Statistics.
[6] BBS 2002. Bangladesh Bureau of Statistics.
[7] BBS 2006. Bangladesh Bureau of Statistics.
[8] BBS-Bangladesh Bureau of Statistics 1998. The Bangladesh census of agriculture and livestock-1983/84. Vol. 1. Structure of Agricultural holding and livestock population Statistical Division, Ministry of Planning, GOB, Dhaka.
[9] Birhanu 2014. Effects of Buserelin Acetate on Conception rate in Repeat Breeding Dairy Cattle.ndian Journal of Animal Reproduction 35.
[10] Birhanu2015. Effects of Gonadotropin Releasing Hormone analogue in Enhancements of Pregnancy in repeat Breeding Dairy cows in and around Mekelle, Tigray, Ethiopia. Animal and Veterinary Sciences. 3: 12-17.
[11] Butler W R2005. Nutrition, negative energy balance and fertility in the postpartum dairy cows. Cattle Practice 13: 13-18.
[12] Butler W R 2000. Nutritional interactions with reproductive performance in dairy cattle. Animal Reproduion Science. 60–61:449–457.
[13] Butler W R and R D Smith 1989. Interrelationships between energy balance and postpartum reproductive function in dairy cattle. Journal of Dairy Science 72:767–783.
[14] Cline A M2002. Efficiency of Synthetic Gonadotropin Releasing Hormone analogue for the control of ovulation during estrus synchronization protocols. Animal Science. Physiology of Reproduction.
[15] Conception rates in Holstein cows treated with GnRH or hCG on the fifth day post AI during summer. Veterinary Zoology. 60: 580-586.
[16] Coulson A D E Noakes, J Hamer, and T Cockril. 1980. Effect of gonadotrophin releasing hormone on levels of luteinising hormone in cattle synchronised with dinoprost. Vet. Rec. 107:108–109.
[17] Dewan ML and Rahman MM 1987. Studies on relation of placenta in Savar dairy farm. Bangladesh Veterinary Journal. 21: 49-53.
[18] DLS- Directorate of Livestock Services. 1991. A report of livestock. Directorate of Livestock Services, Dhaka.
[19] DLS-Directorate of Livestock Services. 1998. A report of livestock. Directorate of Livestock Services, Dhaka.
[20] Dougall S, Williamson N and Macmillan K1995. GNRH induces ovulation of dominant follicle in primiparous dairy cows undergoing anovulatory follicle turn over. Animal ReproductionScience. 39: 205-214.
[21] Douglas WS 1998. Use of GnRH to enhance pregnancy rates and shorten the postpartum interest us interval in dairy cattle. Ohio Veterinary Newsletter. 25: 4–6.
[22] Driancourt MA 2001.egulation of ovarian follicular dynamics in farm animals. Theriogenology. 55: 1211-1239.
[23] Ehlers K, Halvorson L and Glob liber 2013.onadotropin Releasing Hormone and Gonadotropin Releasing Hormone Receptors. ISSN: 1756-2228.
[24] F Lopez-Gatius, p santolaria, A Martino, F Deletang, F De Rensis 2006. The effects of GnRH treatment at the time of AI and 12 days later on reproductive performance of high producing dairy cows during the warm season in northeastern Spain. Theriogenology 65: 820-830.
[25] Faisal Omer Ahmed 2015.anagement of Postpartum Reproductive Performance with Administration of GNRH or PGF2α in Cross Bred Dairy Cows. Journal of Agriculture and Veterinary Science. 8: 45-49.
[26] Foote R2002. The history of Artificial Insemination, Selected notes and notables, American society of Dairy Sciences.
[27] Frick MP 2005. Systematic synchronization and resynchronization systems for reproductive management of lactating dairy cows. Proceeding of the 4th Annual Arizona Dairy Production Conference, October 11, Tempe, AZ, USA. PP: 33.
[28] Ginther OJ2000. Selection of the dominant follicle in cattle‖. Biology of Reproduction. 62: 920-927.
[29] Gustafsson H, Larsso, H Kindah and A Madei1986. Sequential Endocrine Changes and Behavior during estrus and met estrus in repeat breeder and virgin heifer’s. Animal ReproductionScience.10:261.
[30] Gustafsson, H., K. Larsson, H. Kindahl, and A. Madej. 1986. Sequential endocrine changes and behaviour during oestrus and metoestrus in repeat breeders and virgin heifers. Animal Reproduction Science. 10:261–273.
[31] Habib MA 2001. Study on the Red Chittagong Cattle –an animal Genetic Resource of Bangladesh. M. Thesis. Department of Animal Breeding and Genetics, Faculty of Animal Husbandary, BAU, Mymensingh, Bangladesh.
[32] Iftikhar A2009. Improvement of Conception rate in Crossbred Cattle Using GnRH Analogue Therapy. Pakistan Veterinary Journal. 29: 93-94.
[33] Jaswal2013. The effect of administration of gonadotropin releasing hormone analogue at estrus or during luteal phase on reproductive performance of dairy cows maintained under sub-temperate climate. Iranian Journal of Veterinary Research. 14: 57-60.
[34] Jilek F, Pytloun P, Kubesova M, Stipkova M, Bouska J, Volek J, Frelich J, Rajmon R2008. Relationships among body condition score, milk yield and reproduction in Czech Fleckvieh cows. Czech Journal of Animal Science. 53: 357–367.
[35] Kaim M, Bloch A, Wolfenson D, Brawtal R, Rosenberg M, Voet H, Folman Y 2003. Effects of GnRH administered to cows at the onset of estrus on timing of ovulation, endocrine responses, and conception. Journal of Dairy Science. 86: 2012–2021.
[36] Kokkonen T 2005.nergy and protein nutrition of dairy cows during the dry period and early lactation: Production performance and adaptation from pregnancy to lactation. Academic dissertation for PhD, Faculty of Agriculture and Forestry, University of Helsinki, Finland.
[37] Larson S F, W R Butler, and W B Currie 1997. Reduced fertility associated with low progesterone postbreeding and increased milk urea nitrogen in lactating cows. Journal of Dairy Science. 80:1288–1295.
[38] Lee C N, J K Kritser, and R L Ax 1985. Changes of luteinizing hormone and progesterone for dairy cows after gonadotropinreleasing hormone at first postpartum breeding. Journal of Dairy Science. 68:1463–1470.
[39] Lopez-Gatius F, Santolaria P, Martino A, Deletang F, Rensis FD 2006. The effects of GnRH treatment at the time of AI and 12 days later on reproductive performance of high producing dairy cows during the warm season in northeastern Spain. Theriogenology. 65: 820–830.
[40] Lucy M C, and J S Stevenson 1986. Gonadotrophin-releasing hormone at estrus: Luteinizing hormone, estradiol and progesterone during the periestrual and postinsemination periods in dairycattle. Biology of Reproduction. 35:300–311.
[41] LuigjTurmalaj 2014. Role of GnRH on Ovulation during summer’s Months in Cows. Anglisticum Journal. Volume: 3.
[42] MP Beltran2008. Conception rate in Holstein cows treated with GnRH or hCG on the fifth day post artificial insemination during summer. Veterinary Zootecnology. 60: 3.
[43] Mahesh S Dodamani 2010. Evaluation of Pre and Post Artificial Insemination effect of GnRH Hormone on conception of repeat breeder Deoni Cows. Veterinary World. 3: 209-211.
[44] Mann GE and Lamming GE 2001. Relationship between the maternal endocrine environment, early embryo development and the inhibition of the luteolytic mechanism in the cow. Reproduction. 121: 175-180.
[45] McDougall S, Willamson NB and MacMillan KL 1995. GnRH induces ovulation of dominant follicle in primiparous dairy cows. Animal Reproduction Science. 39: 205-214.
[46] Mee MO, Stevenson JS, Scoby RK1990: Influence of gonadotropin-releasing hormone and timing of insemination relative to estrus on pregnancy rates of dairy cattle at first service. Journal of Dairy Science. 73: 1500–1507.
[47] Mee M O, J S Stevenson, B M Alexander, and R G Sasser 1993. Administration of GnRH at estrus influences pregnancy rates, serum concentration of LH, FSH, estradiol-17β, pregnancy specific protein B, and progesterone, proportion of luteal cell types, and in vitro production of progesterone in dairy cows. Journal Animal Science. 71:185–198.
[48] Mee M O, J S Stevenson, R K Scoby and Y Folman 1990. Influence of gonadotrophin-releasing hormone and timing of insemination relative to estrus on pregnancy rates of dairy cattleat first service. Journal Dairy Science. 73:1500–1507.
[49] Mehedi MH 2002. Improvement of conception in repeat breeding cows using synthetic GNRH. MS Thesis. Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh, Bangladesh.
[50] Mehni SB, Shabankareh HK, Kazemi- Bonchenari M and Eghbali M 2012. The comparison of treating Holstein dairy cows with progesterone, CIDR and GnRH after insemination on serum progesterone and pregnancy rates. Reproduction of Domestic Animal. 47: 131-134.
[51] Mollah WA, Rahman A, Alam MGS, Sen MM, &Islam A 1989. Studies on bovine fertility, infertility and health status in an area of Bangladesh. Livestock Advisor. 14: 27-33.
[52] Moreira F, C Risco, M F A Pires, J D Ambrose, M Drost, M DeLorenzo, and W W Thatcher 2000. Effect of body condition on reproductive efficiency of lactating dairy cows receiving a timed insemination. Theriogenology. 53:1305–1319.
[53] Morrel M 2011. Artificial Insemination; Current and the Future Trends: Swedish University of Agricultural Science, Uppsala, Sweden.
[54] Morrow A D1986. Current Therapy in Theriogenology. 243-246, 247-249.
[55] Nebel R L and McGilliard M L 1993.nteractions of high milk yield and reproductive performance of dairy cows. Journal of Dairy Science. 76: 3257-3268.
[56] Opsomer G, Mijten P, Coryn M, Druif ADE 1996. Post partumanestrous in dairy cows. Veterinary Quarterly. 18:68-75.
[57] Osawa T, Nakao T, Kimura M, Kaneko K, Takagi H, Moriyoshi M and Kawata K 1995. Fertirelin and Buserelin compared by LH release, milk progesterone and subsequent reproductive performance in dairy cows treated for follicular cysts. Theriogenology. 44: 835-847.
[58] Osterman S2003. Extended calving interval and increased milking frequency in dairy cows. Doctoral thesis, SwedishUniversity of Agricultural Sciences, Department of Animal Nutrition and Management, Uppsala, swedeen.
[59] P K Sarker 2015. Effects of GnRH Analogue and Vitamin AD3E on Induction of Cyclicity in Anoestrus Heifers. Bangladesh Journal of Vererinary Medicine.13: 33-40.
[60] Perry GA, Perry BL 2009. GnRH treatment at artificial insemination in beef cattle fails to increase plasma progesterone concentrations or pregnancy rates. Theriogenology. 71: 775–779.
[61] Perry GA and Perry BL 2009. Gonadotrophin releasing hormone treatment at artificial insemination in beef cattle fails to increase plasma progesterone concentrations or pregnancy rates. Theriogenology. 71: 775-779.
[62] Rahman MF, Hossain MS and Prodhan MAM 1993. Reproductive problems of cattle in Bangladesh. Bangladesh VeterinaryJournal. 10: 71-73.
[63] Roche F J2001. Follicular growth and hormonal control. Animal Reproduction and Fertility. 17-25.
[64] Rosenberg M, S Y Chun, M Kaim, Z Herz, and Y Folman 1991. The effect of GnRH administered to dairy cows during oestrus on plasma LH and conception in relation to the time of treatment and insemination. Animal Reproduction Science. 24:13–24.
[65] Rosenberg RM, Wolfenson D and Bloch2003. Effects of GnRH administered to cause at the onset of estrus on timing of ovulation and conception. Journal Dairy Science. 86: 2012-2021.
[66] Ryan D P, S Snijders, T Condon, M Grealy, J Sreenan, and K J O Farrell 1994.ndocrine and ovarian responses and pregnancy rates in dairy cows following the administration of gonadotrophin releasing hormone analog at the time of artificial insemination or at mid-cycle post insemination. Animal Reproduction Science. 34:179–191.
[67] Saacke R G, J C Dalton, S Nadir, R L Nebel, and J H Bame 2000. Relationship of seminal traits and insemination time to fertilization rate and embryo quality. Animal Reproduction Science. 60– 61:663–677.
[68] Sali G 1996. Teriogenologia bovine.164-173.
[69] Samad MA 2001. PoshuPalon O Chiskistsavidya. LEP Publications. 2nd edition, BAU, Mymensingh. pp:747-750.
[70] Sarder MJU 2008. Occurrence of reproductive disorders in crossbred cows of north Bangladesh. Published in proceeding paper of 15th International Congress on Biotechnology and animal Reproduction, Bangladesh Agricultural University, Mymensingh, Bangladesh. P:74-75.
[71] Schams D, Shellenberger E, Hoffman B and Karg H 1977. The oestrous cycle of the cow: hormonal parameters and time relationships concerning oestrus, ovulation, and electrical resistance of the vaginal mucus. ActaEndocrinol. 86: 180-192.
[72] Shahneh AZ, Mohammadi Z, Fazeli H, Babak MMS and Dirandeh E 2008. The effect of gonadotrophin releasing hormone injection on plasma progesterone concentration, conception rate and ovulation rate in Iranian Holstein cows. Journal of Animal Veterinary Advance. 7: 1137-1140.
[73] Shamsuddin M, Alam MGS and Ahmed JU 1988. Reproductive disorder of cross bred cows. Bangladesh Veterinary Journal. 22:21-28.
[74] Shamsuddin M, Bhuyan MMU, Sikder TK, Sugulle AH, Chandra PK and Alam MGS 2001. Constraints limiting the efficiency of artificial insemination of cattle in Bangladesh. International Atomic Energy Agency. 1220:9-27.
[75] Short R., Bellows R A, Staigmiller R B, Berardinelli J G and Custer E E 1990. Physiological mechanism controlling anestrus and fertility in postpartum beef cattle. Journal of Animal Science. 68: 799-816.
[76] Sporndly R1995. Feed Tables for Ruminants. Rapport 235.epartment of Animal Nutrition and Management.wedish University of Agricultural Sciences, (SLU), Uppsala, Sweden.
[77] Szenci O, Takacs E, Sulon J, Melode SN and Beckers JF 2006. Evaluation of GnRH treatment 12 days after AI in the reproductive performance of dairy cows Theriogenology. 66: 1811-1815.
[78] Tenhagen B A, Birkelbach E and Heuwieser W 2000.erum progesterone-levels in postpartum dairy cows after repeated application of the prostaglandin F2, analogue D (+) cloprostenol.merican Journal of Veterinary Medicine.47: 213-220.
[79] Ullah G, J W Fuquay, T Keawkhong, B L Clark, D E Pogue, and E J Murphey 1996. Effect of gonadotrophin-releasing hormone at estrus on subsequent luteal function and fertility in lactating Holsteins during heat stress. Journal of Dairy Science. 79:1950–1953
[80] Vacek M, Stadnik L, Stipkova M 2007. Relationships between the incidence of health disorders and the re-production traits of Holstein cows in the Czech Republic. Czech Journal of Animal Science. 52: 227–235.
[81] Wildman E E, Jons P, WagnerR L, Bornan H F and Leseli T N 1992. Dairy cow body condition scoring system and its relationship to selected produced characteristics. Journalof dairy Science. 65: 455–459.
[82] Willard S, Gandy S, Bowers S, Graves K, Elias A and Whisnant C 2003. The effects of GnRH administration post insemination on serum concentrations of progesterone and pregnancy rates in dairy cattle exposed to mild summer heat stress. Theriogenology. 59: 1799-1810.
[83] Yaniz, JL, Murugavel K and Lopez-Gatius F 2004. Recent developments in oestrous synchronization of postpartum dairy cows with and without ovarian disorders. Reproduction of Domestic Animal. 39: 86-93.
[84] Youngquist RS 1998. Anoestrus and infertility in cow. In: Fertility and infertility in Veterinary Practice. 4th edition, Edited by Laing JA Morgan WJB, Wagner WC, Bailliere and Tindall, London pp: 100-105.
[85] Youngquist S R 1887. Current Therapy in Large Animal Theriogenology.
Author Information
  • School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Japan; Department of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh

Cite This Article
  • APA Style

    Sm. Ahasanul Hamid, S. M. Kamruzzaman. (2018). Effects of GnRH on Conception Rate at the Time of Artificial Insemination in Crossbred Dairy Cows. International Journal of Animal Science and Technology, 1(1), 19-34. https://doi.org/10.11648/j.ijast.20170101.14

    Copy | Download

    ACS Style

    Sm. Ahasanul Hamid; S. M. Kamruzzaman. Effects of GnRH on Conception Rate at the Time of Artificial Insemination in Crossbred Dairy Cows. Int. J. Anim. Sci. Technol. 2018, 1(1), 19-34. doi: 10.11648/j.ijast.20170101.14

    Copy | Download

    AMA Style

    Sm. Ahasanul Hamid, S. M. Kamruzzaman. Effects of GnRH on Conception Rate at the Time of Artificial Insemination in Crossbred Dairy Cows. Int J Anim Sci Technol. 2018;1(1):19-34. doi: 10.11648/j.ijast.20170101.14

    Copy | Download

  • @article{10.11648/j.ijast.20170101.14,
      author = {Sm. Ahasanul Hamid and S. M. Kamruzzaman},
      title = {Effects of GnRH on Conception Rate at the Time of Artificial Insemination in Crossbred Dairy Cows},
      journal = {International Journal of Animal Science and Technology},
      volume = {1},
      number = {1},
      pages = {19-34},
      doi = {10.11648/j.ijast.20170101.14},
      url = {https://doi.org/10.11648/j.ijast.20170101.14},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijast.20170101.14},
      abstract = {The present study was conducted to investigate the influence of GnRH (Gonadotropin Releasing Hormone) administration on conception rate at the time of artificial insemination (AI) in healthy crossbred dairy cows. A total 90 cows were recruited and a preset questionnaire was answered by the owners. The cows were divided randomly in to two equal groups. GnRH treated group was injected intramuscularly (i/m) in the neck muscle with GnRH (Gonadorelin Acetate) at the time of AI. Again the treated group was divided randomly into three equal groups (15 cows each) for three different doses of GnRH (200, 250, 300 µg). The necessary information was taken from the owner with pretested questionnaire. The overall 71.11% conception rate was found in GnRH treated group on the other hands 55.56% conception rate was in control groups and having significant effect (P<0.05). There were significant effect (P<0.05) of age and milk yield and there were no significant effect of doses, timing, body condition score and parity within GnRH treated group. The study revealed that use of GnRH improved the conception rate in crossbred dairy cows at the time of AI.},
     year = {2018}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Effects of GnRH on Conception Rate at the Time of Artificial Insemination in Crossbred Dairy Cows
    AU  - Sm. Ahasanul Hamid
    AU  - S. M. Kamruzzaman
    Y1  - 2018/01/03
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ijast.20170101.14
    DO  - 10.11648/j.ijast.20170101.14
    T2  - International Journal of Animal Science and Technology
    JF  - International Journal of Animal Science and Technology
    JO  - International Journal of Animal Science and Technology
    SP  - 19
    EP  - 34
    PB  - Science Publishing Group
    SN  - 2640-1312
    UR  - https://doi.org/10.11648/j.ijast.20170101.14
    AB  - The present study was conducted to investigate the influence of GnRH (Gonadotropin Releasing Hormone) administration on conception rate at the time of artificial insemination (AI) in healthy crossbred dairy cows. A total 90 cows were recruited and a preset questionnaire was answered by the owners. The cows were divided randomly in to two equal groups. GnRH treated group was injected intramuscularly (i/m) in the neck muscle with GnRH (Gonadorelin Acetate) at the time of AI. Again the treated group was divided randomly into three equal groups (15 cows each) for three different doses of GnRH (200, 250, 300 µg). The necessary information was taken from the owner with pretested questionnaire. The overall 71.11% conception rate was found in GnRH treated group on the other hands 55.56% conception rate was in control groups and having significant effect (P<0.05). There were significant effect (P<0.05) of age and milk yield and there were no significant effect of doses, timing, body condition score and parity within GnRH treated group. The study revealed that use of GnRH improved the conception rate in crossbred dairy cows at the time of AI.
    VL  - 1
    IS  - 1
    ER  - 

    Copy | Download

  • Sections