<i>BRAF</i><sup>V600E</sup> Mutation in Gliomas in Iraqi Patients Immunohistochemical Study

Zahraa Marwan Shaban; Salim Rashid Al-Aubaidy

doi:doi:10.11648/j.ijcocr.20170206.11

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BRAF^V600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study

Zahraa Marwan Shaban, Salim Rashid Al-Aubaidy

Received: 11 October 2017 Accepted: 27 October 2017 Published: 3 December 2017

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Abstract

Gliomas considered the most common primary malignant brain tumors in adults. BRAF^V600E mutation occurs more in pediatric gliomas but less frequent in adult gliomas. This study aims to assess the frequency of BRAF mutation in Iraqi patients with gliomas by immunohistochemical study and to correlate its immunoreactivity with some clinicopathological parameters This work did on formalin fixed, paraffin embedded tumor tissue took from 66 patients with different grades of intracranial gliomas of both gender and all age groups in the Baghdad city. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, that was manual tissue microarray, in which twenty-four small cores (each measure 2mm) of represented tissue make, and then cut by microtome. Immunohistochemical detection of BRAF^V600E antibody did by Dako autostainer link 48. BRAF^V600E expressed as cytoplasmic staining in seven (10.60%) cases of glioma. It detected in pleomorphic xanthastrocytoma, oligodendroglioma, anaplastic astrocytoma, anaplastic oligodendroglioma, anaplastic oligoastrocytoma and primary glioblastoma (100%, 9.1% 8.6%, 20.0%, 25% and 6.7%). It had a strong association with pediatric gliomas. From the work concluded that BRAF^V600E mutation occurred in low percentage in gliomas especially adult types and significantly express in pediatric gliomas and some rare glial tumors.

DOI	10.11648/j.ijcocr.20170206.11
Published in	International Journal of Clinical Oncology and Cancer Research (Volume 2, Issue 6, December 2017)
Page(s)	123-128
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

BRAF, Adults, Pediatric, Manual Tissue Microarray, Glioma, Immunohistochemical Study

References

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APA Style

Zahraa Marwan Shaban, Salim Rashid Al-Aubaidy. (2017). BRAFV600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study. International Journal of Clinical Oncology and Cancer Research, 2(6), 123-128. https://doi.org/10.11648/j.ijcocr.20170206.11

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ACS Style

Zahraa Marwan Shaban; Salim Rashid Al-Aubaidy. BRAFV600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study. Int. J. Clin. Oncol. Cancer Res. 2017, 2(6), 123-128. doi: 10.11648/j.ijcocr.20170206.11

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AMA Style

Zahraa Marwan Shaban, Salim Rashid Al-Aubaidy. BRAFV600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study. Int J Clin Oncol Cancer Res. 2017;2(6):123-128. doi: 10.11648/j.ijcocr.20170206.11

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@article{10.11648/j.ijcocr.20170206.11,
  author = {Zahraa Marwan Shaban and Salim Rashid Al-Aubaidy},
  title = {BRAFV600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study},
  journal = {International Journal of Clinical Oncology and Cancer Research},
  volume = {2},
  number = {6},
  pages = {123-128},
  doi = {10.11648/j.ijcocr.20170206.11},
  url = {https://doi.org/10.11648/j.ijcocr.20170206.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcocr.20170206.11},
  abstract = {Gliomas considered the most common primary malignant brain tumors in adults. BRAFV600E mutation occurs more in pediatric gliomas but less frequent in adult gliomas. This study aims to assess the frequency of BRAF mutation in Iraqi patients with gliomas by immunohistochemical study and to correlate its immunoreactivity with some clinicopathological parameters This work did on formalin fixed, paraffin embedded tumor tissue took from 66 patients with different grades of intracranial gliomas of both gender and all age groups in the Baghdad city. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, that was manual tissue microarray, in which twenty-four small cores (each measure 2mm) of represented tissue make, and then cut by microtome. Immunohistochemical detection of BRAFV600E antibody did by Dako autostainer link 48. BRAFV600E expressed as cytoplasmic staining in seven (10.60%) cases of glioma. It detected in pleomorphic xanthastrocytoma, oligodendroglioma, anaplastic astrocytoma, anaplastic oligodendroglioma, anaplastic oligoastrocytoma and primary glioblastoma (100%, 9.1% 8.6%, 20.0%, 25% and 6.7%). It had a strong association with pediatric gliomas. From the work concluded that BRAFV600E mutation occurred in low percentage in gliomas especially adult types and significantly express in pediatric gliomas and some rare glial tumors.},
 year = {2017}
}

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TY  - JOUR
T1  - BRAFV600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study
AU  - Zahraa Marwan Shaban
AU  - Salim Rashid Al-Aubaidy
Y1  - 2017/12/03
PY  - 2017
N1  - https://doi.org/10.11648/j.ijcocr.20170206.11
DO  - 10.11648/j.ijcocr.20170206.11
T2  - International Journal of Clinical Oncology and Cancer Research
JF  - International Journal of Clinical Oncology and Cancer Research
JO  - International Journal of Clinical Oncology and Cancer Research
SP  - 123
EP  - 128
PB  - Science Publishing Group
SN  - 2578-9511
UR  - https://doi.org/10.11648/j.ijcocr.20170206.11
AB  - Gliomas considered the most common primary malignant brain tumors in adults. BRAFV600E mutation occurs more in pediatric gliomas but less frequent in adult gliomas. This study aims to assess the frequency of BRAF mutation in Iraqi patients with gliomas by immunohistochemical study and to correlate its immunoreactivity with some clinicopathological parameters This work did on formalin fixed, paraffin embedded tumor tissue took from 66 patients with different grades of intracranial gliomas of both gender and all age groups in the Baghdad city. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, that was manual tissue microarray, in which twenty-four small cores (each measure 2mm) of represented tissue make, and then cut by microtome. Immunohistochemical detection of BRAFV600E antibody did by Dako autostainer link 48. BRAFV600E expressed as cytoplasmic staining in seven (10.60%) cases of glioma. It detected in pleomorphic xanthastrocytoma, oligodendroglioma, anaplastic astrocytoma, anaplastic oligodendroglioma, anaplastic oligoastrocytoma and primary glioblastoma (100%, 9.1% 8.6%, 20.0%, 25% and 6.7%). It had a strong association with pediatric gliomas. From the work concluded that BRAFV600E mutation occurred in low percentage in gliomas especially adult types and significantly express in pediatric gliomas and some rare glial tumors.
VL  - 2
IS  - 6
ER  -

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Author Information

Zahraa Marwan Shaban

Pathology Department and Forensic Medicine, College of Medicine, University of Mosul, Mosul, Iraq
Salim Rashid Al-Aubaidy

Pathology Department and Forensic Medicine, College of Medicine, University of Baghdad, Baghdad, Iraq

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Plain Text BibTeX RIS

APA Style

Zahraa Marwan Shaban, Salim Rashid Al-Aubaidy. (2017). BRAFV600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study. International Journal of Clinical Oncology and Cancer Research, 2(6), 123-128. https://doi.org/10.11648/j.ijcocr.20170206.11

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ACS Style

Zahraa Marwan Shaban; Salim Rashid Al-Aubaidy. BRAFV600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study. Int. J. Clin. Oncol. Cancer Res. 2017, 2(6), 123-128. doi: 10.11648/j.ijcocr.20170206.11

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AMA Style

Zahraa Marwan Shaban, Salim Rashid Al-Aubaidy. BRAFV600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study. Int J Clin Oncol Cancer Res. 2017;2(6):123-128. doi: 10.11648/j.ijcocr.20170206.11

Copy | Download

@article{10.11648/j.ijcocr.20170206.11,
  author = {Zahraa Marwan Shaban and Salim Rashid Al-Aubaidy},
  title = {BRAFV600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study},
  journal = {International Journal of Clinical Oncology and Cancer Research},
  volume = {2},
  number = {6},
  pages = {123-128},
  doi = {10.11648/j.ijcocr.20170206.11},
  url = {https://doi.org/10.11648/j.ijcocr.20170206.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcocr.20170206.11},
  abstract = {Gliomas considered the most common primary malignant brain tumors in adults. BRAFV600E mutation occurs more in pediatric gliomas but less frequent in adult gliomas. This study aims to assess the frequency of BRAF mutation in Iraqi patients with gliomas by immunohistochemical study and to correlate its immunoreactivity with some clinicopathological parameters This work did on formalin fixed, paraffin embedded tumor tissue took from 66 patients with different grades of intracranial gliomas of both gender and all age groups in the Baghdad city. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, that was manual tissue microarray, in which twenty-four small cores (each measure 2mm) of represented tissue make, and then cut by microtome. Immunohistochemical detection of BRAFV600E antibody did by Dako autostainer link 48. BRAFV600E expressed as cytoplasmic staining in seven (10.60%) cases of glioma. It detected in pleomorphic xanthastrocytoma, oligodendroglioma, anaplastic astrocytoma, anaplastic oligodendroglioma, anaplastic oligoastrocytoma and primary glioblastoma (100%, 9.1% 8.6%, 20.0%, 25% and 6.7%). It had a strong association with pediatric gliomas. From the work concluded that BRAFV600E mutation occurred in low percentage in gliomas especially adult types and significantly express in pediatric gliomas and some rare glial tumors.},
 year = {2017}
}

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TY  - JOUR
T1  - BRAFV600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study
AU  - Zahraa Marwan Shaban
AU  - Salim Rashid Al-Aubaidy
Y1  - 2017/12/03
PY  - 2017
N1  - https://doi.org/10.11648/j.ijcocr.20170206.11
DO  - 10.11648/j.ijcocr.20170206.11
T2  - International Journal of Clinical Oncology and Cancer Research
JF  - International Journal of Clinical Oncology and Cancer Research
JO  - International Journal of Clinical Oncology and Cancer Research
SP  - 123
EP  - 128
PB  - Science Publishing Group
SN  - 2578-9511
UR  - https://doi.org/10.11648/j.ijcocr.20170206.11
AB  - Gliomas considered the most common primary malignant brain tumors in adults. BRAFV600E mutation occurs more in pediatric gliomas but less frequent in adult gliomas. This study aims to assess the frequency of BRAF mutation in Iraqi patients with gliomas by immunohistochemical study and to correlate its immunoreactivity with some clinicopathological parameters This work did on formalin fixed, paraffin embedded tumor tissue took from 66 patients with different grades of intracranial gliomas of both gender and all age groups in the Baghdad city. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, that was manual tissue microarray, in which twenty-four small cores (each measure 2mm) of represented tissue make, and then cut by microtome. Immunohistochemical detection of BRAFV600E antibody did by Dako autostainer link 48. BRAFV600E expressed as cytoplasmic staining in seven (10.60%) cases of glioma. It detected in pleomorphic xanthastrocytoma, oligodendroglioma, anaplastic astrocytoma, anaplastic oligodendroglioma, anaplastic oligoastrocytoma and primary glioblastoma (100%, 9.1% 8.6%, 20.0%, 25% and 6.7%). It had a strong association with pediatric gliomas. From the work concluded that BRAFV600E mutation occurred in low percentage in gliomas especially adult types and significantly express in pediatric gliomas and some rare glial tumors.
VL  - 2
IS  - 6
ER  -

Copy | Download