The Effect of Leaf Extract of Centella Asiatica on Neurogenesis and Bdnf Level in Hippocampus Cell Culture in Young Mice
Clinical Neurology and Neuroscience
Volume 1, Issue 1, February 2017, Pages: 14-19
Received: Jan. 5, 2017; Accepted: Jan. 21, 2017; Published: Mar. 15, 2017
Views 1386      Downloads 83
Emma Kamelia, Departement of Health, Polytechnic of Health Tasikmalaya, Tasikmalaya, West Java, Indonesia
Hadiyat Miko, Departement of Health, Polytechnic of Health Tasikmalaya, Tasikmalaya, West Java, Indonesia
Marni Br Karo, Midwifery Program of Medistra Health Higher School, Jakarta, Indonesia
Andi Asadul Islam, Molecular Biology and Immunology Laboratory for Infection Diseases, Faculty of Medicine, Hasanuddin University Makassar, Indonesia
Mochammad Hatta, Molecular Biology and Immunology Laboratory for Infection Diseases, Faculty of Medicine, Hasanuddin University Makassar, Indonesia
Article Tools
Follow on us
In many world, research of centella asiatica (CA) leaf extracts are used to treat neuronal disease with stem cell or cell line, but in the present study we try to make from hippocampus tissue culture. Methods: Young femalemice dowleyspraque types age of 2 weeks, as many as eighteen (18) tails each taken from its hippocampal tissue then cultured using a medium Roswell Park Memorial Institute (RPMI). Eighteen (18) culture of nerve cells exposed to the extract of CA at a concentration of 0.25 ug / ml (CA A) and eighteen (18) culture of nerve cells exposed to the extract of CA at a concentration of 0.50 ug / ml (CA B), where as eighteen (18) other nerve cell cultures not exposed (control). Then calculated the number of cells per field view and BDNF levels measured in the culture of the three populations. Results: (1) Anumber of larger nerve cells in cell cultures of hippocampal tissue of young rats were fed by CA extracts dose of 0.50 ug / ml compared to 0.25 ug / ml and the control group (p <0.05); that new neurons plays an important role in sinaptogenesis circuit in order to improve the integration function of neurons and synapses that still exist between new and mature neurons in the hippocampus [1]. Conclusion: (2) Levels of BDNF CA dose of 0.50 ug / ml higher than 0.25 mg/ml CA doses but lower than in the controls (p <0.05), that the effect of the extract CA neurogenesis does occur in particular BDNF through the expression of BDNF, the possibility of glutamate in CA extracts is not able to increase the level of GABA in the brain due to activation of GABA synapses on a young neuron is depolarization where the concentration of intracellular Cl ion is high because channel expression Cl exporter KCC2 which slowed (delayed) [2].
Centella Asiatica (CA), Brain Derived Neurotrophic Factor (BDNF), Neurogenesis
To cite this article
Emma Kamelia, Hadiyat Miko, Marni Br Karo, Andi Asadul Islam, Mochammad Hatta, The Effect of Leaf Extract of Centella Asiatica on Neurogenesis and Bdnf Level in Hippocampus Cell Culture in Young Mice, Clinical Neurology and Neuroscience. Vol. 1, No. 1, 2017, pp. 14-19. doi: 10.11648/j.cnn.20170101.14
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Schinder AF, Gage FH. 2004. A hypothesis about the role of adult neurogenesis in hippocampal function. Int Union physiolSci/Am physiolSoc 2004; 19: 253-261.
Ben Ary Y. 2002. Excitatory actions of GABA during development: the nature of the nurture. Nature review neurosci.
Duman RS. 2002. Mood and anxiety disorder. J Am Acad child Adolesc Psychiatry. 41 (6): 745-748.
Snyder JS, Kee N, and Wojtowics. 2001. Effects of adult neurogenesis on synaptic plasticity in the rat dentate gyrus. J Neurophysiology; 85 (6): 2420-2431.
Morris, R. G. M., E. I. Moser, G. Riedel, S. J. Martin, J. Sandhin, M. Day, and C. O’Carroll. 2003. Elements of a neurobiological theory of the hippocampus the role of activity dependent synaptic plasticity in memory. Philosophical transactions of the royal society of London. Series B, Biological sciences, vol. 358 (1432): 773-86.
Yamada K, M. mizuno, and T. Nabeshima. 2002. Role for brain derived neurotrophic factor in learning and memory. Life sciences 70 (7) (January 4): 735-44.
Leibrock J, F. lottspeich, A. Hohn, M. Hofer, B. Hengerer, P. Masia-kowski, H. Thoenen, and Y-A. Barde. 1989. Molecular cloning and expression of brain derived neurotrophic factor. Nature, vol. 341: 149-52.
Matson MP, Chan SL, and Duan W. 2002. Modification of brain aging and neurodegenerative disorder by genes, diet and behavior. Physiol rev; 82: 637-672.
Kashmira J, Gohil JAP, Anuradha K, Gajjar. 2010. Pharmacological review on centellaasiatica: A potential herbal cure-all, Indian J Pharm Sci. Vol. 72: pp 546-556.
Zheng C, and L. Qin. 2007. Chemicalcomponents of centellaasiatica and their bioactivities. Journal of Chinese integrative medicine, Vol. 5: 348-351.
Pitella F, R. C. Dutra, D. D. Junior, M. T. P. Lopes, and N. R. Barbosa. 2009. Antioxidant and Cytotoxic activities of centellaasiatica (L) Urb. International journal of molecular sciences 10 (9) (September): 3713-21.
Krishnamurthy, G. Rajanikant, M. C. Senut, D. Zemke, J. Min, B. Frenkel, E. J. greenberg, S. W. Yu. 2010. Asiatic acid, a pentacyclictriterpene from centellaasiatica is neuroprotective in a mouse model of focal cerebral ischemia. Journal of neuroscience research 87 (11): 2541-2550.
Winarto WP and Surbakti M. 2003. Kasiat-dan manfaat tanaman pegagan. Cetakanke 1. Agromediapustaka. Jakarta 2003.
Inamdar PK, et al. Determination of biologically active constituents in centellaasiatica. Journal of chromatography 1996; 742: 127-130.
Nalini K, Arroor AR, KaranthKS, and Rao A. 1992. Effect of centellaasiatica fresh leaf aqueous extract on learning and memory and biogenic amine turnover in albino rats. Fitoterapia LXIII: 232-7.
Centella Herba. 1998. WHO monographs on selected medicinal plants.
Obrietan K, Gao XB, and Anthony N. 2002. Excitatory actions of GABA increase BDNF expression via a MAPK-CREB dependent mechanism a positive feedback circuit in developing neurons; 88: 1005-1015.
Gibbs RB. 1998. Level of trk-b and BDNF mRNA, but not NGF mRNA, fluctuate across the estrous cycle and increase in response to acute hormone replacement. Brain Res; 787: 259-268.
Yamada MK. 2002. Brain Derived Neurotrophic Factor promotes the maturation of GABAergic mechanism in cultured hippocampal neuron. J. Neurosc; 22: 7580-7585.
Beckinschtein P, Cammarota M, Katche C, Clipczuk L, Rossato JI, Goldin A, Izquierdo I, Medina JH. 2008. BDNF is essential to promote persistence of long-term memory storage, ProcNatclAcadSci USA, Vol 105; pp 2711-2716.
The European agency for the evaluation of medicinal products. 1998. Committee for veterinary medicinal products centellaasiaticaeextractum. Summary report veterinary medicines evaluation unit EMEA/MRL/494/98-final.
Freshney RI. 2000. Culture of animal cell: a manual ofbasic technique. 4th ed. New York: Wiley-Liss.
Morgan SL, Darling DC. 1993. Animal cell culture. Oxford: BIOS Scientific Publisher.
Subakir SB. 2003. Cell culture, option program international class. Faculty of Medicine University of Indonesia: 1-10.
Elfving B, P. H Plougman and G Wegener. 2010. Detection of Brain derived neurotrophic factor (BDNF) in rat blood and brain preparation using Elisa: Pitfalls and solutions. Journal of neuroscience methods 187 (1) (March 15): 73-7.
Haeun M, Jin Tae Hong, MiiHee Park. 2015. Centellaasiatica enhances neurogenesis and protects neuronal cells against H2O2 induced oxidative injury. Journal of biomedical research vol. 16. Pp 121-128. DOI:
GilvaloisL, Marmigere F, F Rage, G. Ixart, S Arancibia and L. Tapia-Arancibia. 2001. Immobilization stress rapidly and differentially modulates BDNF and TrkB mRNA expression in the Pituitary gland of adult malerats. Neuroendocrinology. Sep; 74 (3): 148-59.
Nibuya M, M. Takahashi, D. S. Russell, and R. S. Duman. 1999. Repeated stress increases catalytic TrkB mRNA in rat hippocampus. Neurosci Lett. May 267 (2): 81-4.
Mizuno M, K. Yamada, N. Takei, M. H. Tran, J. He, A. Nakajima, H. Nawa, and T. Nabeshima. 2003. Phosphatidylinositol 3-kinase: a Molecule mediating BDNF-dependent spatial memory formation. Molecular Psychiatry 8 (2) (March): 217-24.
Ning Y, Huang J, Kalionis B, Bian Q, Dong J, Wu J, Tai X, Xia S, Shen Z. 2015. Oleanolic acid induces differentiation of neural stem cells to neurons: an involvement of transcription factor Nkx-2,5. Stem cells int. Vol. 2015. pp 672312-672323.
Kumar A, A. Prakash, and S. Dogra. 2011. Centellaasiatica attenuates D-galaktose induced cognitive impairment, oxidative strss and mitochondrial dysfunction in mice. International kournal of alzheimer’s disease, vol. 2011, p. 347569.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186