The Effect of Leaf Extract of Centella Asiatica on Neurogenesis and Bdnf Level in Hippocampus Cell Culture in Young Mice
Clinical Neurology and Neuroscience
Volume 1, Issue 1, February 2017, Pages: 14-19
Received: Jan. 5, 2017; Accepted: Jan. 21, 2017; Published: Mar. 15, 2017
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Emma Kamelia, Departement of Health, Polytechnic of Health Tasikmalaya, Tasikmalaya, West Java, Indonesia
Hadiyat Miko, Departement of Health, Polytechnic of Health Tasikmalaya, Tasikmalaya, West Java, Indonesia
Marni Br Karo, Midwifery Program of Medistra Health Higher School, Jakarta, Indonesia
Andi Asadul Islam, Molecular Biology and Immunology Laboratory for Infection Diseases, Faculty of Medicine, Hasanuddin University Makassar, Indonesia
Mochammad Hatta, Molecular Biology and Immunology Laboratory for Infection Diseases, Faculty of Medicine, Hasanuddin University Makassar, Indonesia
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In many world, research of centella asiatica (CA) leaf extracts are used to treat neuronal disease with stem cell or cell line, but in the present study we try to make from hippocampus tissue culture. Methods: Young femalemice dowleyspraque types age of 2 weeks, as many as eighteen (18) tails each taken from its hippocampal tissue then cultured using a medium Roswell Park Memorial Institute (RPMI). Eighteen (18) culture of nerve cells exposed to the extract of CA at a concentration of 0.25 ug / ml (CA A) and eighteen (18) culture of nerve cells exposed to the extract of CA at a concentration of 0.50 ug / ml (CA B), where as eighteen (18) other nerve cell cultures not exposed (control). Then calculated the number of cells per field view and BDNF levels measured in the culture of the three populations. Results: (1) Anumber of larger nerve cells in cell cultures of hippocampal tissue of young rats were fed by CA extracts dose of 0.50 ug / ml compared to 0.25 ug / ml and the control group (p <0.05); that new neurons plays an important role in sinaptogenesis circuit in order to improve the integration function of neurons and synapses that still exist between new and mature neurons in the hippocampus [1]. Conclusion: (2) Levels of BDNF CA dose of 0.50 ug / ml higher than 0.25 mg/ml CA doses but lower than in the controls (p <0.05), that the effect of the extract CA neurogenesis does occur in particular BDNF through the expression of BDNF, the possibility of glutamate in CA extracts is not able to increase the level of GABA in the brain due to activation of GABA synapses on a young neuron is depolarization where the concentration of intracellular Cl ion is high because channel expression Cl exporter KCC2 which slowed (delayed) [2].
Centella Asiatica (CA), Brain Derived Neurotrophic Factor (BDNF), Neurogenesis
To cite this article
Emma Kamelia, Hadiyat Miko, Marni Br Karo, Andi Asadul Islam, Mochammad Hatta, The Effect of Leaf Extract of Centella Asiatica on Neurogenesis and Bdnf Level in Hippocampus Cell Culture in Young Mice, Clinical Neurology and Neuroscience. Vol. 1, No. 1, 2017, pp. 14-19. doi: 10.11648/j.cnn.20170101.14
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