Immune-Related Gene Spatzle4 and Its Differential Immune Responses against Microbes in the Silkworm, Bombyx Mori

Ying Xu; Ji Liu; Fengpeng Li; Xuefang Wang; Xihai Li; Zhongyuan Shen; Jinmei Wu

doi:doi:10.11648/j.ajcem.20150306.14

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Immune-Related Gene Spatzle4 and Its Differential Immune Responses against Microbes in the Silkworm, Bombyx Mori

Ying Xu, Ji Liu, Fengpeng Li, Xuefang Wang, Xihai Li, Zhongyuan Shen, Jinmei Wu

Received: 11 December 2015 Accepted: Published: 11 December 2015

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Abstract

Spatzle is a key factor in Toll signaling pathway against microbe invasion. The spatzle4 gene from silkworm (Bmspz4) was successfully cloned in this study, it is the second cloned and studied gene in the Spz family besides Bmspz1 in the silkworm and it was cloned for the first time in silkworm integument. The spatzle4 gene expression was analyzed in different tissues of the third day ﬁfth instar larvae and the highest expression was detected in the head, and secondly in integument. Microbe infection showed that BmSpz4 participated in immune response. The transcriptional expression of BmSpz4 was induced differentially between Gram-negative bacteria and Gram-positive bacteria or fungi. This study showed that Bmspz4 gene plays an important role in the innate immunity of integument of silkworm, Bombyx mori.

DOI	10.11648/j.ajcem.20150306.14
Published in	American Journal of Clinical and Experimental Medicine (Volume 3, Issue 6, November 2015)
Page(s)	344-349
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Spatzle, Immune Response, Silkworm, Immune-Related Gene, Microbe

References

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APA Style

Ying Xu, Ji Liu, Fengpeng Li, Xuefang Wang, Xihai Li, et al. (2015). Immune-Related Gene Spatzle4 and Its Differential Immune Responses against Microbes in the Silkworm, Bombyx Mori. American Journal of Clinical and Experimental Medicine, 3(6), 344-349. https://doi.org/10.11648/j.ajcem.20150306.14

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ACS Style

Ying Xu; Ji Liu; Fengpeng Li; Xuefang Wang; Xihai Li, et al. Immune-Related Gene Spatzle4 and Its Differential Immune Responses against Microbes in the Silkworm, Bombyx Mori. Am. J. Clin. Exp. Med. 2015, 3(6), 344-349. doi: 10.11648/j.ajcem.20150306.14

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AMA Style

Ying Xu, Ji Liu, Fengpeng Li, Xuefang Wang, Xihai Li, et al. Immune-Related Gene Spatzle4 and Its Differential Immune Responses against Microbes in the Silkworm, Bombyx Mori. Am J Clin Exp Med. 2015;3(6):344-349. doi: 10.11648/j.ajcem.20150306.14

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@article{10.11648/j.ajcem.20150306.14,
  author = {Ying Xu and Ji Liu and Fengpeng Li and Xuefang Wang and Xihai Li and Zhongyuan Shen and Jinmei Wu},
  title = {Immune-Related Gene Spatzle4 and Its Differential Immune Responses against Microbes in the Silkworm, Bombyx Mori},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {3},
  number = {6},
  pages = {344-349},
  doi = {10.11648/j.ajcem.20150306.14},
  url = {https://doi.org/10.11648/j.ajcem.20150306.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150306.14},
  abstract = {Spatzle is a key factor in Toll signaling pathway against microbe invasion. The spatzle4 gene from silkworm (Bmspz4) was successfully cloned in this study, it is the second cloned and studied gene in the Spz family besides Bmspz1 in the silkworm and it was cloned for the first time in silkworm integument. The spatzle4 gene expression was analyzed in different tissues of the third day ﬁfth instar larvae and the highest expression was detected in the head, and secondly in integument. Microbe infection showed that BmSpz4 participated in immune response. The transcriptional expression of BmSpz4 was induced differentially between Gram-negative bacteria and Gram-positive bacteria or fungi. This study showed that Bmspz4 gene plays an important role in the innate immunity of integument of silkworm, Bombyx mori.},
 year = {2015}
}

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TY  - JOUR
T1  - Immune-Related Gene Spatzle4 and Its Differential Immune Responses against Microbes in the Silkworm, Bombyx Mori
AU  - Ying Xu
AU  - Ji Liu
AU  - Fengpeng Li
AU  - Xuefang Wang
AU  - Xihai Li
AU  - Zhongyuan Shen
AU  - Jinmei Wu
Y1  - 2015/12/11
PY  - 2015
N1  - https://doi.org/10.11648/j.ajcem.20150306.14
DO  - 10.11648/j.ajcem.20150306.14
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 344
EP  - 349
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20150306.14
AB  - Spatzle is a key factor in Toll signaling pathway against microbe invasion. The spatzle4 gene from silkworm (Bmspz4) was successfully cloned in this study, it is the second cloned and studied gene in the Spz family besides Bmspz1 in the silkworm and it was cloned for the first time in silkworm integument. The spatzle4 gene expression was analyzed in different tissues of the third day ﬁfth instar larvae and the highest expression was detected in the head, and secondly in integument. Microbe infection showed that BmSpz4 participated in immune response. The transcriptional expression of BmSpz4 was induced differentially between Gram-negative bacteria and Gram-positive bacteria or fungi. This study showed that Bmspz4 gene plays an important role in the innate immunity of integument of silkworm, Bombyx mori.
VL  - 3
IS  - 6
ER  -

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Author Information

Ying Xu

College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang City, P. R. China
Ji Liu

College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang City, P. R. China
Fengpeng Li

College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang City, P. R. China
Xuefang Wang

College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang City, P. R. China
Xihai Li

College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang City, P. R. China
Zhongyuan Shen

The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang City, P. R. China
Jinmei Wu

College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang City, P. R. China; The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang City, P. R. China

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APA Style

Ying Xu, Ji Liu, Fengpeng Li, Xuefang Wang, Xihai Li, et al. (2015). Immune-Related Gene Spatzle4 and Its Differential Immune Responses against Microbes in the Silkworm, Bombyx Mori. American Journal of Clinical and Experimental Medicine, 3(6), 344-349. https://doi.org/10.11648/j.ajcem.20150306.14

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ACS Style

Ying Xu; Ji Liu; Fengpeng Li; Xuefang Wang; Xihai Li, et al. Immune-Related Gene Spatzle4 and Its Differential Immune Responses against Microbes in the Silkworm, Bombyx Mori. Am. J. Clin. Exp. Med. 2015, 3(6), 344-349. doi: 10.11648/j.ajcem.20150306.14

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AMA Style

Ying Xu, Ji Liu, Fengpeng Li, Xuefang Wang, Xihai Li, et al. Immune-Related Gene Spatzle4 and Its Differential Immune Responses against Microbes in the Silkworm, Bombyx Mori. Am J Clin Exp Med. 2015;3(6):344-349. doi: 10.11648/j.ajcem.20150306.14

Copy | Download

@article{10.11648/j.ajcem.20150306.14,
  author = {Ying Xu and Ji Liu and Fengpeng Li and Xuefang Wang and Xihai Li and Zhongyuan Shen and Jinmei Wu},
  title = {Immune-Related Gene Spatzle4 and Its Differential Immune Responses against Microbes in the Silkworm, Bombyx Mori},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {3},
  number = {6},
  pages = {344-349},
  doi = {10.11648/j.ajcem.20150306.14},
  url = {https://doi.org/10.11648/j.ajcem.20150306.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150306.14},
  abstract = {Spatzle is a key factor in Toll signaling pathway against microbe invasion. The spatzle4 gene from silkworm (Bmspz4) was successfully cloned in this study, it is the second cloned and studied gene in the Spz family besides Bmspz1 in the silkworm and it was cloned for the first time in silkworm integument. The spatzle4 gene expression was analyzed in different tissues of the third day ﬁfth instar larvae and the highest expression was detected in the head, and secondly in integument. Microbe infection showed that BmSpz4 participated in immune response. The transcriptional expression of BmSpz4 was induced differentially between Gram-negative bacteria and Gram-positive bacteria or fungi. This study showed that Bmspz4 gene plays an important role in the innate immunity of integument of silkworm, Bombyx mori.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Immune-Related Gene Spatzle4 and Its Differential Immune Responses against Microbes in the Silkworm, Bombyx Mori
AU  - Ying Xu
AU  - Ji Liu
AU  - Fengpeng Li
AU  - Xuefang Wang
AU  - Xihai Li
AU  - Zhongyuan Shen
AU  - Jinmei Wu
Y1  - 2015/12/11
PY  - 2015
N1  - https://doi.org/10.11648/j.ajcem.20150306.14
DO  - 10.11648/j.ajcem.20150306.14
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 344
EP  - 349
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20150306.14
AB  - Spatzle is a key factor in Toll signaling pathway against microbe invasion. The spatzle4 gene from silkworm (Bmspz4) was successfully cloned in this study, it is the second cloned and studied gene in the Spz family besides Bmspz1 in the silkworm and it was cloned for the first time in silkworm integument. The spatzle4 gene expression was analyzed in different tissues of the third day ﬁfth instar larvae and the highest expression was detected in the head, and secondly in integument. Microbe infection showed that BmSpz4 participated in immune response. The transcriptional expression of BmSpz4 was induced differentially between Gram-negative bacteria and Gram-positive bacteria or fungi. This study showed that Bmspz4 gene plays an important role in the innate immunity of integument of silkworm, Bombyx mori.
VL  - 3
IS  - 6
ER  -

Copy | Download