Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs

Kolawole Victor Olorunshola; Cheh Augustine Awasum; Nenadi Claire Hedima

doi:doi:10.11648/j.ajcem.20140206.14

| Peer-Reviewed

Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs

Kolawole Victor Olorunshola, Cheh Augustine Awasum, Nenadi Claire Hedima

Published in American Journal of Clinical and Experimental Medicine (Volume 2, Issue 6)

Received: 29 September 2014 Accepted: 7 November 2014 Published: 17 November 2014

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

This study was aimed at evaluating the effect of caffeine on biliary secretions in indigenous Nigerian dogs. A total of 6 dogs weighing 12 – 15 kg divided into 2 groups were used. The control group was fed their normal diet and water ad libitum and the treated group received 16 mg/kg of white crystalline caffeine dissolved in 10 mls of water and administered orally 8 hours prior to each surgery. Under sodium thiopentone and ketamine anaesthesia, common bile duct cannulation was done by the modified method of Rath and Hutchison. Bile was collected immediately post cannulation over a period of 48 to 72 hours. The bile samples from all dogs were analysed for bile volume, pH and electrolyte concentrations. The results showed significant increase in the bile volume in the caffeine treated group: 3.41 ± 0.85 ml compared to the control group: 1.24 ± 0.17 ml (p<0.05). The bile pH in the caffeine treated group: 7.40 ± 0.24 was significantly higher than the control group: 6.68 ± 0.18 (p< 0.05). The potassium concentration of 6.08 ± 0.49mmol/L in control group was significantly higher than the potassium concentration of 4.81 ± 0.21mmol/L in the treated group (p< 0.05). However, there was no significant change in the concentration of bicarbonate, chloride and sodium ions in the caffeine treated animals. We conclude that orally administered caffeine significantly increased bile volume and bile PH and significantly decreased bile potassium concentration in indigenous Nigerian dogs and these findings may have implication for digestion and absorption of fat soluble vitamins and a measure of liver functions.

Published in	American Journal of Clinical and Experimental Medicine (Volume 2, Issue 6)
DOI	10.11648/j.ajcem.20140206.14
Page(s)	132-136
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Caffeine, Bile Volume, Bile Secretion, Liver Function, Fat Digestion and Absorption, Indigenous Nigerian Dogs

References

[1]	Barabote, R. D., Tamang, D. G. and Abeywardena, S.N. (2006). Extra domains in secondary transport carriers and channel proteins. Biochim. Biophys. Acta 1758 (10): 1557–79.
[2]	Bergstrom, K. and Thulin, L. (1989).Effects of somatostatin on hepatic bile formation. Gastroenterology, 96: 206-212.
[3]	Bowen, R. (2001). Secretion of Bile and the Role of Bile Acids n Digestion. Colorado State Hyper textbook article on Bile. 15: 1-19. (Retrieved 2007).
[4]	Burwen, S.J., Schmucker, D.L. and Jones, A. (1992). Subcellular and molecular mechanisms of bile secretion. Int. Rev. Cytol. 135 :269-313.
[5]	Dhingra, R., Sullivan, L., Jacques, P.F., Wang, T.J., Fox, C.S., Meigs, J.B., D'Agostino, R.B., Gaziano, J.M. and Vasan R.S. (2007). Soft drink consumption and risk of developing cardiometabolic risk factors and the metabolic syndrome in middle-aged adults in the community. Circulation. 116(5):480-8.
[6]	Duane, W.C., Gilberstadt, M.L. and Wiegand, D.M. (1979). Diurnal rhythms of bile acid production in the rat. Am. J. Physiol. 236:R175-R179.
[7]	Duncan and Prasse (2011). Reference Values in Clinical Pathology, 4th Ed. Pp 331 – 338. Latimer, Mahaffey and Prasse.
[8]	Farouk, M., Geoghegan, J.G. and Pruthi, R.S.,Thomson, H.J., Pappas, T.N. and Meyers, W.C. (1992). Intracerebroventricular neuropeptide Y stimulates bile secretion via vagal mechanism. Gut, 33:1562-1565.
[9]	Fisone, G., Borgkvist, A. And Usiello, A. (2004). Caffeine as a psychomotor stimulant: Mechanisms of action. Cellular and Molecular Life Sciences 61:857-872.
[10]	Ganong, W.F. (2005). Review of Medical Physiology, 22nd Ed. Pp 473-504. The McGraw Hill Companies Inc. New York.
[11]	Gebhard, R.L. and Prigge, W.F. (1992). Thyroid hormone differentially auguments biliary sterol secretion in the rat. II. The chronic bile fistula model. Journal of Lipid Research 33:1467-1473.
[12]	Juliano, L.M. and Griffiths, R.R. (2005). Caffeine. In Lowinson, J.H., Ruiz, P., Millman, R.B., Langrod, J.G. (Eds.). Substance Abuse: A Comprehensive Textbook, Fourth Edition. (pp 403-421). Baltimore: Lippincott, Williams, & Wilkins.
[13]	Lautt, W. W. (1997). Hepatic vasculature: a conceptual review. Gastroenterology, 73:1163-1169.
[14]	Leonard, R.J., Fayez, K.G., Juanita, L.M., Hamid, M.S. and Jackie, D.W.(1981). Physiology of the Gastrointestinal tract, Vol.2, Raven Press, ISBN 0890044406/9780890044407
[15]	Maldonado-Valderrama, J., Wilde, P., Macierzanka, A. and Mackie A.(2011) The role of bile salts in digestion. Advanced Colloid Interface of Scencei. 9, 165(1):36-46.
[16]	Maton, A., Hopkins, J., McLaughlin, C.W., Johnson, S., Warner, M. Q., LaHart, D. and Wright, D. (1993). Human Biology and Health. Englewood Cliffs, New Jersey, USA: Prentice Hall. ISBN 0-13-981176-1.
[17]	McGowan, C. C., Cover, T. L. and Blaser, M. J. (1996). Helicobacter pylori and gastric acid: biological and therapeutic implications. Gastroenterology 110, 926–938.
[18]	Nakano, A., Tietz, P.S. and LaRusso, N.F (1990). Circadian rhythms of biliary protein and lipid excretion in rats. Am. J. Physiol. 258:G653-G659.
[19]	Nyberg, B. (1990). Bile secretion in man. The effects of somatostatin, vasoactive intestinal peptide and secretin. Acta Chir Scand Suppl, 557: 1-40.
[20]	Omar, M.E.A.S, Ayman, R.B. and Siham, M.E (2005). Effect of cysteamine on bile secretion in the rat. Pharmacological Reports 57:345-351.
[21]	Pocock, G. And Richards, C.D. (2006).The regulation of bile secretion, In Human Physiology: The Basis of Medicine, 3rd Edition, Oxford University Press, pp 405-407.
[22]	Rath, L. and Hutchison, M. (1989). A new method of bile duct cannulation allowing bile collection and re-infusion in the conscious rat. Laboratory Animals 23, 163-168.
[23]	Sai, J. K., Suyama, M., Kubokawa, Y., Matsumura,Y., Inami, K., Watanabe, S. and Kirino, E. (2010). Identification of cerebral response to balloon distention of the bile duct. World J Gastroenterol. 16(14):1772-5.
[24]	Steve, J. M. and Roger, A. B. (2006). Canine Extrahepatic Biliary Tract Disease and Surgery, Compendium. (4) 303.
[25]	Tennant, S. M., Hartland, E. L., Phumoonna, T., Lyras, D., Rood, J. I., Robins-Browne, R. M. and Van-Driel, I. R. (2008). Influence of gastric acid on susceptibility to infection with ingested bacterial pathogens. Infect Immun 76, 639–645.
[26]	The Vaults of Erowid. (2006). Caffeine Content of Beverages, Foods and Medications. http://www.erowid.org/chemicals/caffeine/caffeine_info1.shtml. Retrieved 2009-08-03. (Accessed 19/09/2012, 10.23pm)
[27]	Van - Wijk, H., Donachie, P., Mann, D.L., McMahon, H, and Robb, D. (2001). A novel bile duct cannulation method with tail cuff exteriorization allowing continuous intravenous infusion and enterohepatic recirculation in the unrestrained rat. Laboratory Animals journal. Volume 35, Number 4 :325-333.
[28]	World Health Organization. (2003). Media centre news release, (Retrieved 2011).
[29]	Zelber-Sagi, S., Nitzan-Kaluski, D., Goldsmith, R., Webb, M., Blendis, L., Halpern, Z. and Oren, R. (2007). Long term nutritional intake and the risk for non-alcoholic fatty liver disease (NAFLD): a population based study. Journal of Hepatology; 47(5):711-7.

Cite This Article

Plain Text BibTeX RIS

APA Style

Kolawole Victor Olorunshola, Cheh Augustine Awasum, Nenadi Claire Hedima. (2014). Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs. American Journal of Clinical and Experimental Medicine, 2(6), 132-136. https://doi.org/10.11648/j.ajcem.20140206.14

Copy | Download

ACS Style

Kolawole Victor Olorunshola; Cheh Augustine Awasum; Nenadi Claire Hedima. Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs. Am. J. Clin. Exp. Med. 2014, 2(6), 132-136. doi: 10.11648/j.ajcem.20140206.14

Copy | Download

AMA Style

Kolawole Victor Olorunshola, Cheh Augustine Awasum, Nenadi Claire Hedima. Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs. Am J Clin Exp Med. 2014;2(6):132-136. doi: 10.11648/j.ajcem.20140206.14

Copy | Download

@article{10.11648/j.ajcem.20140206.14,
  author = {Kolawole Victor Olorunshola and Cheh Augustine Awasum and Nenadi Claire Hedima},
  title = {Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {2},
  number = {6},
  pages = {132-136},
  doi = {10.11648/j.ajcem.20140206.14},
  url = {https://doi.org/10.11648/j.ajcem.20140206.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20140206.14},
  abstract = {This study was aimed at evaluating the effect of caffeine on biliary secretions in indigenous Nigerian dogs. A total of 6 dogs weighing 12 – 15 kg divided into 2 groups were used. The control group was fed their normal diet and water ad libitum and the treated group received 16 mg/kg of white crystalline caffeine dissolved in 10 mls of water and administered orally 8 hours prior to each surgery. Under sodium thiopentone and ketamine anaesthesia, common bile duct cannulation was done by the modified method of Rath and Hutchison. Bile was collected immediately post cannulation over a period of 48 to 72 hours. The bile samples from all dogs were analysed for bile volume, pH and electrolyte concentrations. The results showed significant increase in the bile volume in the caffeine treated group: 3.41 ± 0.85 ml compared to the control group: 1.24 ± 0.17 ml (p<0.05). The bile pH in the caffeine treated group: 7.40 ± 0.24 was significantly higher than the control group: 6.68 ± 0.18 (p< 0.05). The potassium concentration of 6.08 ± 0.49mmol/L in control group was significantly higher than the potassium concentration of 4.81 ± 0.21mmol/L in the treated group (p< 0.05). However, there was no significant change in the concentration of bicarbonate, chloride and sodium ions in the caffeine treated animals. We conclude that orally administered caffeine significantly increased bile volume and bile PH and significantly decreased bile potassium concentration in indigenous Nigerian dogs and these findings may have implication for digestion and absorption of fat soluble vitamins and a measure of liver functions.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs
AU  - Kolawole Victor Olorunshola
AU  - Cheh Augustine Awasum
AU  - Nenadi Claire Hedima
Y1  - 2014/11/17
PY  - 2014
N1  - https://doi.org/10.11648/j.ajcem.20140206.14
DO  - 10.11648/j.ajcem.20140206.14
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 132
EP  - 136
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20140206.14
AB  - This study was aimed at evaluating the effect of caffeine on biliary secretions in indigenous Nigerian dogs. A total of 6 dogs weighing 12 – 15 kg divided into 2 groups were used. The control group was fed their normal diet and water ad libitum and the treated group received 16 mg/kg of white crystalline caffeine dissolved in 10 mls of water and administered orally 8 hours prior to each surgery. Under sodium thiopentone and ketamine anaesthesia, common bile duct cannulation was done by the modified method of Rath and Hutchison. Bile was collected immediately post cannulation over a period of 48 to 72 hours. The bile samples from all dogs were analysed for bile volume, pH and electrolyte concentrations. The results showed significant increase in the bile volume in the caffeine treated group: 3.41 ± 0.85 ml compared to the control group: 1.24 ± 0.17 ml (p<0.05). The bile pH in the caffeine treated group: 7.40 ± 0.24 was significantly higher than the control group: 6.68 ± 0.18 (p< 0.05). The potassium concentration of 6.08 ± 0.49mmol/L in control group was significantly higher than the potassium concentration of 4.81 ± 0.21mmol/L in the treated group (p< 0.05). However, there was no significant change in the concentration of bicarbonate, chloride and sodium ions in the caffeine treated animals. We conclude that orally administered caffeine significantly increased bile volume and bile PH and significantly decreased bile potassium concentration in indigenous Nigerian dogs and these findings may have implication for digestion and absorption of fat soluble vitamins and a measure of liver functions.
VL  - 2
IS  - 6
ER  -

Copy | Download

Author Information

Kolawole Victor Olorunshola

Department of Human Physiology, College of Health Sciences, University of Abuja, Abuja, Nigeria
Cheh Augustine Awasum

Department of Veterinary Surgery and Medicine / Veterinary Teaching Hospital, Ahmadu Bello University, Zaria, Nigeria
Nenadi Claire Hedima

Department of Veterinary Surgery and Medicine / Veterinary Teaching Hospital, Ahmadu Bello University, Zaria, Nigeria

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Kolawole Victor Olorunshola, Cheh Augustine Awasum, Nenadi Claire Hedima. (2014). Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs. American Journal of Clinical and Experimental Medicine, 2(6), 132-136. https://doi.org/10.11648/j.ajcem.20140206.14

Copy | Download

ACS Style

Kolawole Victor Olorunshola; Cheh Augustine Awasum; Nenadi Claire Hedima. Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs. Am. J. Clin. Exp. Med. 2014, 2(6), 132-136. doi: 10.11648/j.ajcem.20140206.14

Copy | Download

AMA Style

Kolawole Victor Olorunshola, Cheh Augustine Awasum, Nenadi Claire Hedima. Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs. Am J Clin Exp Med. 2014;2(6):132-136. doi: 10.11648/j.ajcem.20140206.14

Copy | Download

@article{10.11648/j.ajcem.20140206.14,
  author = {Kolawole Victor Olorunshola and Cheh Augustine Awasum and Nenadi Claire Hedima},
  title = {Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {2},
  number = {6},
  pages = {132-136},
  doi = {10.11648/j.ajcem.20140206.14},
  url = {https://doi.org/10.11648/j.ajcem.20140206.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20140206.14},
  abstract = {This study was aimed at evaluating the effect of caffeine on biliary secretions in indigenous Nigerian dogs. A total of 6 dogs weighing 12 – 15 kg divided into 2 groups were used. The control group was fed their normal diet and water ad libitum and the treated group received 16 mg/kg of white crystalline caffeine dissolved in 10 mls of water and administered orally 8 hours prior to each surgery. Under sodium thiopentone and ketamine anaesthesia, common bile duct cannulation was done by the modified method of Rath and Hutchison. Bile was collected immediately post cannulation over a period of 48 to 72 hours. The bile samples from all dogs were analysed for bile volume, pH and electrolyte concentrations. The results showed significant increase in the bile volume in the caffeine treated group: 3.41 ± 0.85 ml compared to the control group: 1.24 ± 0.17 ml (p<0.05). The bile pH in the caffeine treated group: 7.40 ± 0.24 was significantly higher than the control group: 6.68 ± 0.18 (p< 0.05). The potassium concentration of 6.08 ± 0.49mmol/L in control group was significantly higher than the potassium concentration of 4.81 ± 0.21mmol/L in the treated group (p< 0.05). However, there was no significant change in the concentration of bicarbonate, chloride and sodium ions in the caffeine treated animals. We conclude that orally administered caffeine significantly increased bile volume and bile PH and significantly decreased bile potassium concentration in indigenous Nigerian dogs and these findings may have implication for digestion and absorption of fat soluble vitamins and a measure of liver functions.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs
AU  - Kolawole Victor Olorunshola
AU  - Cheh Augustine Awasum
AU  - Nenadi Claire Hedima
Y1  - 2014/11/17
PY  - 2014
N1  - https://doi.org/10.11648/j.ajcem.20140206.14
DO  - 10.11648/j.ajcem.20140206.14
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 132
EP  - 136
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20140206.14
AB  - This study was aimed at evaluating the effect of caffeine on biliary secretions in indigenous Nigerian dogs. A total of 6 dogs weighing 12 – 15 kg divided into 2 groups were used. The control group was fed their normal diet and water ad libitum and the treated group received 16 mg/kg of white crystalline caffeine dissolved in 10 mls of water and administered orally 8 hours prior to each surgery. Under sodium thiopentone and ketamine anaesthesia, common bile duct cannulation was done by the modified method of Rath and Hutchison. Bile was collected immediately post cannulation over a period of 48 to 72 hours. The bile samples from all dogs were analysed for bile volume, pH and electrolyte concentrations. The results showed significant increase in the bile volume in the caffeine treated group: 3.41 ± 0.85 ml compared to the control group: 1.24 ± 0.17 ml (p<0.05). The bile pH in the caffeine treated group: 7.40 ± 0.24 was significantly higher than the control group: 6.68 ± 0.18 (p< 0.05). The potassium concentration of 6.08 ± 0.49mmol/L in control group was significantly higher than the potassium concentration of 4.81 ± 0.21mmol/L in the treated group (p< 0.05). However, there was no significant change in the concentration of bicarbonate, chloride and sodium ions in the caffeine treated animals. We conclude that orally administered caffeine significantly increased bile volume and bile PH and significantly decreased bile potassium concentration in indigenous Nigerian dogs and these findings may have implication for digestion and absorption of fat soluble vitamins and a measure of liver functions.
VL  - 2
IS  - 6
ER  -

Copy | Download