Inhibitory Effect of Ethyl Pyruvate on Orthotopic Transplantation of Gastric Cancer in Severe Combined Immunodeficiency Mice

Tingting Chen; Xiaoxiao Dong; Xiaoyan Zhang

doi:doi:10.11648/j.ajcem.20190702.14

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Inhibitory Effect of Ethyl Pyruvate on Orthotopic Transplantation of Gastric Cancer in Severe Combined Immunodeficiency Mice

Tingting Chen, Xiaoxiao Dong, Xiaoyan Zhang

Published in American Journal of Clinical and Experimental Medicine (Volume 7, Issue 2)

Received: 29 May 2019 Published: 29 July 2019

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Abstract

Objective: To investigate the inhibitory effect and mechanism of ethyl pyruvate (EP) on the growth and liver metastasis of orthotopically transplanted gastric cancer in severe combined immunodeficiency (SCID) mice. Methods: SCID mice were orthotopically transplanted with SGC-7901 human gastric cancer tissue to establish a liver metastasis model of gastric cancer. Animals were injected intraperitoneally with different concentrations of EP. After 30 days, gastric cancer and metastatic liver tissues were taken out to detect the volume and weight of gastric cancer tissues and the number of metastatic liver nodules. Real-time quantitative PCR and immunohistochemistry were used to detect high mobility group protein B in different groups. Expression levels of 1 (HMGB1), receptor glycation end product receptor (RAGE), NF-κB, vascular endothelial growth factor (VEGF), and membrane type 1 matrix metalloproteinase (MT1-MMP). Results: Compared with the control group, the weight and size of gastric cancer tissue and the number of metastatic liver nodules in the EP treatment group were significantly reduced (P<0.01). EP inhibited the expression of HMGB1, RAGE, VEGF and MT1-MMP in gastric cancer and metastatic liver tissue, but had no significant effect on NF-κB expression. Conclusion: EP may inhibit the growth of gastric cancer and liver metastasis in SCID mice by down-regulating HMGB1-RAGE pathway, which may have therapeutic effects on cancer.

Published in	American Journal of Clinical and Experimental Medicine (Volume 7, Issue 2)
DOI	10.11648/j.ajcem.20190702.14
Page(s)	61-65
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Severe Combined Immunodeficiency, Orthotopic Transplantation, Gastric Neoplasms, Tumor Metastasis

References

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APA Style

Tingting Chen, Xiaoxiao Dong, Xiaoyan Zhang. (2019). Inhibitory Effect of Ethyl Pyruvate on Orthotopic Transplantation of Gastric Cancer in Severe Combined Immunodeficiency Mice. American Journal of Clinical and Experimental Medicine, 7(2), 61-65. https://doi.org/10.11648/j.ajcem.20190702.14

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ACS Style

Tingting Chen; Xiaoxiao Dong; Xiaoyan Zhang. Inhibitory Effect of Ethyl Pyruvate on Orthotopic Transplantation of Gastric Cancer in Severe Combined Immunodeficiency Mice. Am. J. Clin. Exp. Med. 2019, 7(2), 61-65. doi: 10.11648/j.ajcem.20190702.14

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AMA Style

Tingting Chen, Xiaoxiao Dong, Xiaoyan Zhang. Inhibitory Effect of Ethyl Pyruvate on Orthotopic Transplantation of Gastric Cancer in Severe Combined Immunodeficiency Mice. Am J Clin Exp Med. 2019;7(2):61-65. doi: 10.11648/j.ajcem.20190702.14

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@article{10.11648/j.ajcem.20190702.14,
  author = {Tingting Chen and Xiaoxiao Dong and Xiaoyan Zhang},
  title = {Inhibitory Effect of Ethyl Pyruvate on Orthotopic Transplantation of Gastric Cancer in Severe Combined Immunodeficiency Mice},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {7},
  number = {2},
  pages = {61-65},
  doi = {10.11648/j.ajcem.20190702.14},
  url = {https://doi.org/10.11648/j.ajcem.20190702.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20190702.14},
  abstract = {Objective: To investigate the inhibitory effect and mechanism of ethyl pyruvate (EP) on the growth and liver metastasis of orthotopically transplanted gastric cancer in severe combined immunodeficiency (SCID) mice. Methods: SCID mice were orthotopically transplanted with SGC-7901 human gastric cancer tissue to establish a liver metastasis model of gastric cancer. Animals were injected intraperitoneally with different concentrations of EP. After 30 days, gastric cancer and metastatic liver tissues were taken out to detect the volume and weight of gastric cancer tissues and the number of metastatic liver nodules. Real-time quantitative PCR and immunohistochemistry were used to detect high mobility group protein B in different groups. Expression levels of 1 (HMGB1), receptor glycation end product receptor (RAGE), NF-κB, vascular endothelial growth factor (VEGF), and membrane type 1 matrix metalloproteinase (MT1-MMP). Results: Compared with the control group, the weight and size of gastric cancer tissue and the number of metastatic liver nodules in the EP treatment group were significantly reduced (PConclusion: EP may inhibit the growth of gastric cancer and liver metastasis in SCID mice by down-regulating HMGB1-RAGE pathway, which may have therapeutic effects on cancer.},
 year = {2019}
}

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TY  - JOUR
T1  - Inhibitory Effect of Ethyl Pyruvate on Orthotopic Transplantation of Gastric Cancer in Severe Combined Immunodeficiency Mice
AU  - Tingting Chen
AU  - Xiaoxiao Dong
AU  - Xiaoyan Zhang
Y1  - 2019/07/29
PY  - 2019
N1  - https://doi.org/10.11648/j.ajcem.20190702.14
DO  - 10.11648/j.ajcem.20190702.14
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 61
EP  - 65
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20190702.14
AB  - Objective: To investigate the inhibitory effect and mechanism of ethyl pyruvate (EP) on the growth and liver metastasis of orthotopically transplanted gastric cancer in severe combined immunodeficiency (SCID) mice. Methods: SCID mice were orthotopically transplanted with SGC-7901 human gastric cancer tissue to establish a liver metastasis model of gastric cancer. Animals were injected intraperitoneally with different concentrations of EP. After 30 days, gastric cancer and metastatic liver tissues were taken out to detect the volume and weight of gastric cancer tissues and the number of metastatic liver nodules. Real-time quantitative PCR and immunohistochemistry were used to detect high mobility group protein B in different groups. Expression levels of 1 (HMGB1), receptor glycation end product receptor (RAGE), NF-κB, vascular endothelial growth factor (VEGF), and membrane type 1 matrix metalloproteinase (MT1-MMP). Results: Compared with the control group, the weight and size of gastric cancer tissue and the number of metastatic liver nodules in the EP treatment group were significantly reduced (PConclusion: EP may inhibit the growth of gastric cancer and liver metastasis in SCID mice by down-regulating HMGB1-RAGE pathway, which may have therapeutic effects on cancer.
VL  - 7
IS  - 2
ER  -

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Author Information

Tingting Chen

Department of Gastroenterology, Binzhou People's Hospital, Binzhou City, P. R. China
Xiaoxiao Dong

Department of Gastroenterology, Binzhou People's Hospital, Binzhou City, P. R. China
Xiaoyan Zhang

Department of Interventional Operating, Binzhou People's Hospital, Binzhou City, P. R. China

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APA Style

Tingting Chen, Xiaoxiao Dong, Xiaoyan Zhang. (2019). Inhibitory Effect of Ethyl Pyruvate on Orthotopic Transplantation of Gastric Cancer in Severe Combined Immunodeficiency Mice. American Journal of Clinical and Experimental Medicine, 7(2), 61-65. https://doi.org/10.11648/j.ajcem.20190702.14

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ACS Style

Tingting Chen; Xiaoxiao Dong; Xiaoyan Zhang. Inhibitory Effect of Ethyl Pyruvate on Orthotopic Transplantation of Gastric Cancer in Severe Combined Immunodeficiency Mice. Am. J. Clin. Exp. Med. 2019, 7(2), 61-65. doi: 10.11648/j.ajcem.20190702.14

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AMA Style

Tingting Chen, Xiaoxiao Dong, Xiaoyan Zhang. Inhibitory Effect of Ethyl Pyruvate on Orthotopic Transplantation of Gastric Cancer in Severe Combined Immunodeficiency Mice. Am J Clin Exp Med. 2019;7(2):61-65. doi: 10.11648/j.ajcem.20190702.14

Copy | Download

@article{10.11648/j.ajcem.20190702.14,
  author = {Tingting Chen and Xiaoxiao Dong and Xiaoyan Zhang},
  title = {Inhibitory Effect of Ethyl Pyruvate on Orthotopic Transplantation of Gastric Cancer in Severe Combined Immunodeficiency Mice},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {7},
  number = {2},
  pages = {61-65},
  doi = {10.11648/j.ajcem.20190702.14},
  url = {https://doi.org/10.11648/j.ajcem.20190702.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20190702.14},
  abstract = {Objective: To investigate the inhibitory effect and mechanism of ethyl pyruvate (EP) on the growth and liver metastasis of orthotopically transplanted gastric cancer in severe combined immunodeficiency (SCID) mice. Methods: SCID mice were orthotopically transplanted with SGC-7901 human gastric cancer tissue to establish a liver metastasis model of gastric cancer. Animals were injected intraperitoneally with different concentrations of EP. After 30 days, gastric cancer and metastatic liver tissues were taken out to detect the volume and weight of gastric cancer tissues and the number of metastatic liver nodules. Real-time quantitative PCR and immunohistochemistry were used to detect high mobility group protein B in different groups. Expression levels of 1 (HMGB1), receptor glycation end product receptor (RAGE), NF-κB, vascular endothelial growth factor (VEGF), and membrane type 1 matrix metalloproteinase (MT1-MMP). Results: Compared with the control group, the weight and size of gastric cancer tissue and the number of metastatic liver nodules in the EP treatment group were significantly reduced (PConclusion: EP may inhibit the growth of gastric cancer and liver metastasis in SCID mice by down-regulating HMGB1-RAGE pathway, which may have therapeutic effects on cancer.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Inhibitory Effect of Ethyl Pyruvate on Orthotopic Transplantation of Gastric Cancer in Severe Combined Immunodeficiency Mice
AU  - Tingting Chen
AU  - Xiaoxiao Dong
AU  - Xiaoyan Zhang
Y1  - 2019/07/29
PY  - 2019
N1  - https://doi.org/10.11648/j.ajcem.20190702.14
DO  - 10.11648/j.ajcem.20190702.14
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 61
EP  - 65
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20190702.14
AB  - Objective: To investigate the inhibitory effect and mechanism of ethyl pyruvate (EP) on the growth and liver metastasis of orthotopically transplanted gastric cancer in severe combined immunodeficiency (SCID) mice. Methods: SCID mice were orthotopically transplanted with SGC-7901 human gastric cancer tissue to establish a liver metastasis model of gastric cancer. Animals were injected intraperitoneally with different concentrations of EP. After 30 days, gastric cancer and metastatic liver tissues were taken out to detect the volume and weight of gastric cancer tissues and the number of metastatic liver nodules. Real-time quantitative PCR and immunohistochemistry were used to detect high mobility group protein B in different groups. Expression levels of 1 (HMGB1), receptor glycation end product receptor (RAGE), NF-κB, vascular endothelial growth factor (VEGF), and membrane type 1 matrix metalloproteinase (MT1-MMP). Results: Compared with the control group, the weight and size of gastric cancer tissue and the number of metastatic liver nodules in the EP treatment group were significantly reduced (PConclusion: EP may inhibit the growth of gastric cancer and liver metastasis in SCID mice by down-regulating HMGB1-RAGE pathway, which may have therapeutic effects on cancer.
VL  - 7
IS  - 2
ER  -

Copy | Download