Chemopreventive Effects of Cocoa Extract Application Towards Expression of Bcl-2 and Mda on Albino Mice Skin Induced by Dmba-Tpa
American Journal of Clinical and Experimental Medicine
Volume 5, Issue 3, May 2017, Pages: 97-101
Received: Mar. 21, 2017; Accepted: Mar. 31, 2017; Published: May 18, 2017
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Authors
Khairuddin Djawad, Department of Dermatology and Venereology, Medical Faculty, Hasanuddin University, Makassar, Indonesia
Munawar Ambar, Department of Dermatology and Venereology, Medical Faculty, Hasanuddin University, Makassar, Indonesia
Siswanto Wahab, Department of Dermatology and Venereology, Medical Faculty, Hasanuddin University, Makassar, Indonesia
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Abstract
This research aims to determine the effect of topical cocoa extract application through ekspression of Bcl-2 and MDA on albino mice skin that induced by DMBA-TPA. The research was conducted at Animal Laboratory for application of DMBA, MDA-TPA and cocoa extract and Biomolecular Laboratory of Medical Faculty of Hasanuddin University Makassar as the place of ELISA examination. The method used was animal experimental with pure experimental design to know the role of topical cocoa extract towards the skin of mice which has been given DMBA-TPA three times a week in 12 weeks. The research unit was the skin of the back side of albino mice which have been excised and divided into 5 groups of treatment. The results indicate that extract of concentration cocoa 400 ppm (p<0.05) which is applied every day has the highest effects decreased of Bcl-2 and MDA expression after being inducted by DMBA-TPA three times a week.
Keywords
Bcl-2, Cacao Extract, DMBA, Malondialdehyde (MDA), TPA
To cite this article
Khairuddin Djawad, Munawar Ambar, Siswanto Wahab, Chemopreventive Effects of Cocoa Extract Application Towards Expression of Bcl-2 and Mda on Albino Mice Skin Induced by Dmba-Tpa, American Journal of Clinical and Experimental Medicine. Vol. 5, No. 3, 2017, pp. 97-101. doi: 10.11648/j.ajcem.20170503.16
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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