Chemopreventive Effects of Cocoa Extract Application Towards Expression of Bcl-2 and Mda on Albino Mice Skin Induced by Dmba-Tpa
American Journal of Clinical and Experimental Medicine
Volume 5, Issue 3, May 2017, Pages: 97-101
Received: Mar. 21, 2017;
Accepted: Mar. 31, 2017;
Published: May 18, 2017
Views 2149 Downloads 95
Khairuddin Djawad, Department of Dermatology and Venereology, Medical Faculty, Hasanuddin University, Makassar, Indonesia
Munawar Ambar, Department of Dermatology and Venereology, Medical Faculty, Hasanuddin University, Makassar, Indonesia
Siswanto Wahab, Department of Dermatology and Venereology, Medical Faculty, Hasanuddin University, Makassar, Indonesia
This research aims to determine the effect of topical cocoa extract application through ekspression of Bcl-2 and MDA on albino mice skin that induced by DMBA-TPA. The research was conducted at Animal Laboratory for application of DMBA, MDA-TPA and cocoa extract and Biomolecular Laboratory of Medical Faculty of Hasanuddin University Makassar as the place of ELISA examination. The method used was animal experimental with pure experimental design to know the role of topical cocoa extract towards the skin of mice which has been given DMBA-TPA three times a week in 12 weeks. The research unit was the skin of the back side of albino mice which have been excised and divided into 5 groups of treatment. The results indicate that extract of concentration cocoa 400 ppm (p<0.05) which is applied every day has the highest effects decreased of Bcl-2 and MDA expression after being inducted by DMBA-TPA three times a week.
Chemopreventive Effects of Cocoa Extract Application Towards Expression of Bcl-2 and Mda on Albino Mice Skin Induced by Dmba-Tpa, American Journal of Clinical and Experimental Medicine.
Vol. 5, No. 3,
2017, pp. 97-101.
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Niedernhofer LJ, Daniels Js, Rouzer CA et. al. Malondialdehyde, a Product of Lipid Peroxidation, is Mutagenic in Human Cells. JBC 278 (33): p. 31426-33.
Muqbil I, Azmi AS, Banu N. Prior exposure to restraint stress enhances 7, 12-dimethylbenz (a) anthracene (DMBA) induced DNA damage in rats, 2006. FEBS Letters 580 (2): p. 3995-3999.
Lukitaningsih E, Noegrohati S.. Studi pemisahan senyawa hidrokarbon poliaromatik secara kromatografi gas kolom kapiler. MFI. 2000. 11 (1): p. 31-38.
Gasser P, Lati E, Peno-Mazzarino L, Bouzoud D, Allegaert L, Bernaert H., Cocoa polyphenolls and their influence on parameters involved in ex vivo skin restructuring. Int J Cosmet Sci. 2008. 30 (5): p. 339-45.
Othman A, Ismail A, Ghani NA, Adenan I, et al. Antioxidant capacity and phenolic content of cocoa beans. Food Chemistry.100: p. 1523–30.
Cooke MS et al., Oxidative DNA damage: mechanism, mutation, and disease. FASEB J. 2003. 17: p. 1195-1214.
Nigam and Shukla,. Preventive Effects of Diallyl Sulfida on 7, 12 dimetilbenz (a) antrasen (DMBA) in Tumor Skin, Mol Nuir Food Res, 2007. 51: p. 1324-26.
Romiro IR, Ramos S, Oliva EL, et al., Cocoa-rich diet prevents azoxymethane-induced colonic preneoplastic lesions in rats by restraining oxidative stress and cell proliferation and inducing apoptosis. Mol Nutr; 2011. 55 (12): p. 1895-9.
Marthin MA, Serrano ABG, Ramos S et al. Protection of Human HepG2 Cells against Oxidative Stress by Cocoa Phenolic Extract. J. Agric. Food Chem, 2008. 56: p. 7765-72.
Zhu QY, Schramm DD, Gross HB, Holt RR, Kim SH, et al., Influence of cocoa flavanols and procyanidins on free radical-induced human erythrocyte hemolysis. Clinical & Developmental Immunology, 2005; 12 (1): p. 27–34.
Grigorof B., Reactive Oxygen Species and Their Relation to Carcinogenesis. Trakia Journal of Sciences. 2012. 10 (3): p. 83-92.
Andujar I, Recio MC, Giner RM, Rios JL. Cocoa polyphenols and their potential benefits for human health. Oxidative medicine and cellular longevity, 2012, p. 1-24.
Ponten F, Berg C, Ahmadian A. Molecular pathology in basal cell cancer with p53 as genetic marker. Oncogen. 1997; 15: p. 1059-67.
Butel JS., Viral Carcinogenesis Revelation of Molecular Mechanism and Etiology of Human Disease. Carcinogenesis. 2000. 21: p. 405.
Bickers DR and Athar M., Oxidative Stress in the Pathogenesis of Skin Diseases. JID. 2006. 126: p. 2565-75.
Afaq F, Katiyar SK, Polyphenols: Skin Photoprotection and Inhibition of Photocarcinogenesis. Mini Rev Med Chem. 2011 11 (14): p. 1200–15.