| Peer-Reviewed

Chemopreventive Effects of Cocoa Extract Application Towards Expression of Bcl-2 and Mda on Albino Mice Skin Induced by Dmba-Tpa

Received: 21 March 2017     Accepted: 31 March 2017     Published: 18 May 2017
Views:       Downloads:
Abstract

This research aims to determine the effect of topical cocoa extract application through ekspression of Bcl-2 and MDA on albino mice skin that induced by DMBA-TPA. The research was conducted at Animal Laboratory for application of DMBA, MDA-TPA and cocoa extract and Biomolecular Laboratory of Medical Faculty of Hasanuddin University Makassar as the place of ELISA examination. The method used was animal experimental with pure experimental design to know the role of topical cocoa extract towards the skin of mice which has been given DMBA-TPA three times a week in 12 weeks. The research unit was the skin of the back side of albino mice which have been excised and divided into 5 groups of treatment. The results indicate that extract of concentration cocoa 400 ppm (p<0.05) which is applied every day has the highest effects decreased of Bcl-2 and MDA expression after being inducted by DMBA-TPA three times a week.

Published in American Journal of Clinical and Experimental Medicine (Volume 5, Issue 3)
DOI 10.11648/j.ajcem.20170503.16
Page(s) 97-101
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Bcl-2, Cacao Extract, DMBA, Malondialdehyde (MDA), TPA

References
[1] Niedernhofer LJ, Daniels Js, Rouzer CA et. al. Malondialdehyde, a Product of Lipid Peroxidation, is Mutagenic in Human Cells. JBC 278 (33): p. 31426-33.
[2] Muqbil I, Azmi AS, Banu N. Prior exposure to restraint stress enhances 7, 12-dimethylbenz (a) anthracene (DMBA) induced DNA damage in rats, 2006. FEBS Letters 580 (2): p. 3995-3999.
[3] Lukitaningsih E, Noegrohati S.. Studi pemisahan senyawa hidrokarbon poliaromatik secara kromatografi gas kolom kapiler. MFI. 2000. 11 (1): p. 31-38.
[4] Gasser P, Lati E, Peno-Mazzarino L, Bouzoud D, Allegaert L, Bernaert H., Cocoa polyphenolls and their influence on parameters involved in ex vivo skin restructuring. Int J Cosmet Sci. 2008. 30 (5): p. 339-45.
[5] Othman A, Ismail A, Ghani NA, Adenan I, et al. Antioxidant capacity and phenolic content of cocoa beans. Food Chemistry.100: p. 1523–30.
[6] Cooke MS et al., Oxidative DNA damage: mechanism, mutation, and disease. FASEB J. 2003. 17: p. 1195-1214.
[7] Nigam and Shukla,. Preventive Effects of Diallyl Sulfida on 7, 12 dimetilbenz (a) antrasen (DMBA) in Tumor Skin, Mol Nuir Food Res, 2007. 51: p. 1324-26.
[8] Romiro IR, Ramos S, Oliva EL, et al., Cocoa-rich diet prevents azoxymethane-induced colonic preneoplastic lesions in rats by restraining oxidative stress and cell proliferation and inducing apoptosis. Mol Nutr; 2011. 55 (12): p. 1895-9.
[9] Marthin MA, Serrano ABG, Ramos S et al. Protection of Human HepG2 Cells against Oxidative Stress by Cocoa Phenolic Extract. J. Agric. Food Chem, 2008. 56: p. 7765-72.
[10] Zhu QY, Schramm DD, Gross HB, Holt RR, Kim SH, et al., Influence of cocoa flavanols and procyanidins on free radical-induced human erythrocyte hemolysis. Clinical & Developmental Immunology, 2005; 12 (1): p. 27–34.
[11] Grigorof B., Reactive Oxygen Species and Their Relation to Carcinogenesis. Trakia Journal of Sciences. 2012. 10 (3): p. 83-92.
[12] Andujar I, Recio MC, Giner RM, Rios JL. Cocoa polyphenols and their potential benefits for human health. Oxidative medicine and cellular longevity, 2012, p. 1-24.
[13] Ponten F, Berg C, Ahmadian A. Molecular pathology in basal cell cancer with p53 as genetic marker. Oncogen. 1997; 15: p. 1059-67.
[14] Butel JS., Viral Carcinogenesis Revelation of Molecular Mechanism and Etiology of Human Disease. Carcinogenesis. 2000. 21: p. 405.
[15] Bickers DR and Athar M., Oxidative Stress in the Pathogenesis of Skin Diseases. JID. 2006. 126: p. 2565-75.
[16] Afaq F, Katiyar SK, Polyphenols: Skin Photoprotection and Inhibition of Photocarcinogenesis. Mini Rev Med Chem. 2011 11 (14): p. 1200–15.
Cite This Article
  • APA Style

    Khairuddin Djawad, Munawar Ambar, Siswanto Wahab. (2017). Chemopreventive Effects of Cocoa Extract Application Towards Expression of Bcl-2 and Mda on Albino Mice Skin Induced by Dmba-Tpa. American Journal of Clinical and Experimental Medicine, 5(3), 97-101. https://doi.org/10.11648/j.ajcem.20170503.16

    Copy | Download

    ACS Style

    Khairuddin Djawad; Munawar Ambar; Siswanto Wahab. Chemopreventive Effects of Cocoa Extract Application Towards Expression of Bcl-2 and Mda on Albino Mice Skin Induced by Dmba-Tpa. Am. J. Clin. Exp. Med. 2017, 5(3), 97-101. doi: 10.11648/j.ajcem.20170503.16

    Copy | Download

    AMA Style

    Khairuddin Djawad, Munawar Ambar, Siswanto Wahab. Chemopreventive Effects of Cocoa Extract Application Towards Expression of Bcl-2 and Mda on Albino Mice Skin Induced by Dmba-Tpa. Am J Clin Exp Med. 2017;5(3):97-101. doi: 10.11648/j.ajcem.20170503.16

    Copy | Download

  • @article{10.11648/j.ajcem.20170503.16,
      author = {Khairuddin Djawad and Munawar Ambar and Siswanto Wahab},
      title = {Chemopreventive Effects of Cocoa Extract Application Towards Expression of Bcl-2 and Mda on Albino Mice Skin Induced by Dmba-Tpa},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {5},
      number = {3},
      pages = {97-101},
      doi = {10.11648/j.ajcem.20170503.16},
      url = {https://doi.org/10.11648/j.ajcem.20170503.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20170503.16},
      abstract = {This research aims to determine the effect of topical cocoa extract application through ekspression of Bcl-2 and MDA on albino mice skin that induced by DMBA-TPA. The research was conducted at Animal Laboratory for application of DMBA, MDA-TPA and cocoa extract and Biomolecular Laboratory of Medical Faculty of Hasanuddin University Makassar as the place of ELISA examination. The method used was animal experimental with pure experimental design to know the role of topical cocoa extract towards the skin of mice which has been given DMBA-TPA three times a week in 12 weeks. The research unit was the skin of the back side of albino mice which have been excised and divided into 5 groups of treatment. The results indicate that extract of concentration cocoa 400 ppm (p<0.05) which is applied every day has the highest effects decreased of Bcl-2 and MDA expression after being inducted by DMBA-TPA three times a week.},
     year = {2017}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Chemopreventive Effects of Cocoa Extract Application Towards Expression of Bcl-2 and Mda on Albino Mice Skin Induced by Dmba-Tpa
    AU  - Khairuddin Djawad
    AU  - Munawar Ambar
    AU  - Siswanto Wahab
    Y1  - 2017/05/18
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajcem.20170503.16
    DO  - 10.11648/j.ajcem.20170503.16
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 97
    EP  - 101
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20170503.16
    AB  - This research aims to determine the effect of topical cocoa extract application through ekspression of Bcl-2 and MDA on albino mice skin that induced by DMBA-TPA. The research was conducted at Animal Laboratory for application of DMBA, MDA-TPA and cocoa extract and Biomolecular Laboratory of Medical Faculty of Hasanuddin University Makassar as the place of ELISA examination. The method used was animal experimental with pure experimental design to know the role of topical cocoa extract towards the skin of mice which has been given DMBA-TPA three times a week in 12 weeks. The research unit was the skin of the back side of albino mice which have been excised and divided into 5 groups of treatment. The results indicate that extract of concentration cocoa 400 ppm (p<0.05) which is applied every day has the highest effects decreased of Bcl-2 and MDA expression after being inducted by DMBA-TPA three times a week.
    VL  - 5
    IS  - 3
    ER  - 

    Copy | Download

Author Information
  • Department of Dermatology and Venereology, Medical Faculty, Hasanuddin University, Makassar, Indonesia

  • Department of Dermatology and Venereology, Medical Faculty, Hasanuddin University, Makassar, Indonesia

  • Department of Dermatology and Venereology, Medical Faculty, Hasanuddin University, Makassar, Indonesia

  • Sections