The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke
American Journal of Clinical and Experimental Medicine
Volume 5, Issue 3, May 2017, Pages: 93-96
Received: Feb. 27, 2017; Accepted: Apr. 5, 2017; Published: May 9, 2017
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Authors
Chitanava Tamara, Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
Egiev Ivan, Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
Chechelian Valerii, Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
Dzhopua Maksim, Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
Trofimenko Artem, Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
Zanin Sergey, Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
Turovaya Alla, Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
Kade Azamat, Department of Common and Clinical Pathophysiology, Federal State Budget Educational Institution of Higher Education “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar, Russia
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Abstract
The effect of D-asparagine administered exogenously on the glial cicatrix formation was studied in experimental spinal stroke model in rats, which was conducted by photothrombosis of vessels in thoracic spinal cord. The experiments were carried out on three groups of animals: group №1 (n = 20) which was a control group; №2 group (n = 20) which included rats with spinal stroke model; group №3 (n = 20) included rats with spinal stroke model that D-Asparagine was administered from 3rd to 6th day of the experiment, with the dosage 21.7 mg/kg for the course of treatment. According to the analysis results of spinal cord micropreparations it is obvious that the density of cell distribution in the spinal cord slices in rats of group №3 (D-asparagine with the dosage 21,7 mg/kg for the course of treatment) is three times lower in comparison with the same index of the rats from group №2 (without D-asparagine), (p≤0,01). Thus, the density of cell distribution in photothrombosis lesions of spinal cord vessels on micropreparations from group №3 (D-asparagine with the dosage 21, 7 mg/kg for the course of treatment) is close to the value from group №1 (control), which means that statistically significant differences between the amount of cells in group №1 (control) and group №3 (administered by D-asparagine) have not been identified (p≥0, 01).
Keywords
D-Asparagine, Glial Cicatrix, Spinal Cord Injury, Photothrombosis, Micropreparations, Rats
To cite this article
Chitanava Tamara, Egiev Ivan, Chechelian Valerii, Dzhopua Maksim, Trofimenko Artem, Zanin Sergey, Turovaya Alla, Kade Azamat, The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke, American Journal of Clinical and Experimental Medicine. Vol. 5, No. 3, 2017, pp. 93-96. doi: 10.11648/j.ajcem.20170503.15
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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