The Protective Effects of Topical Cocoa Extract Lotio on Albino Mice to the Level of 8-OHdG After Ultraviolet B Exposure
American Journal of Clinical and Experimental Medicine
Volume 5, Issue 2, March 2017, Pages: 46-49
Received: Jan. 25, 2017; Accepted: Feb. 10, 2017; Published: Mar. 2, 2017
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Authors
Ramona Utami, Departement of Dermatology and Venereology, Medical Faculty, Hasanudin University, Makassar, Indonesia
Anis Irawan Anwar, Departement of Dermatology and Venereology, Medical Faculty, Hasanudin University, Makassar, Indonesia
Khairuddin Djawad, Departement of Dermatology and Venereology, Medical Faculty, Hasanudin University, Makassar, Indonesia
Anni Adriani, Departement of Dermatology and Venereology, Medical Faculty, Hasanudin University, Makassar, Indonesia
Siswanto Wahab, Departement of Dermatology and Venereology, Medical Faculty, Hasanudin University, Makassar, Indonesia
Burhanuddin Bahar, Departement of Biostatistic, Medical Faculty, Hasanuddin University, Makassar, Indonesia
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Abstract
Ultraviolet B radiation (UVB) often cause oxidative stress, through the reactive oxygen species (ROS) formation and produce DNA damage. This study aims to assess the protective effects of topically cocoa extract lotio against DNA damage by measure of 8-OHdG level in albino mice after UVB exposure. This study was conducted in Makassar, South Sulawesi, using 20 Swiss albino mice, aged 6-9 weeks, weight 20-30 grams. Mice were divided into 3 groups: The first group is a control group or negative control (without cocoa extract and without UVB exposure, consist 4 mice), the second is UVB control group exposure with UVB 450mJ / cm2 three times a week for 12 weeks without topical cocoa extract (4 mice) and the third group is mice with extract cocoa 200, 400 and 800 ppm every day, 20 minutes before exposure with UVB 450mJ / cm2 three times a week for 12 weeks (each concentration of cocoa extracts consist of 4 mice). At 24 hours after last exposure all mice were terminated and excisional biopsy on the dorsal skin of the size of 1x1 cm and performed 8-OHdG ELISA. The results show there was significant difference (p <0.05) between the control group UVB with negative control and cocoa 800 ppm. The 800 ppm cocoa extract is the most effective to the reduction of 8-OhdG level.
Keywords
8-OHdG, Cocoa Extract Lotio, UVB
To cite this article
Ramona Utami, Anis Irawan Anwar, Khairuddin Djawad, Anni Adriani, Siswanto Wahab, Burhanuddin Bahar, The Protective Effects of Topical Cocoa Extract Lotio on Albino Mice to the Level of 8-OHdG After Ultraviolet B Exposure, American Journal of Clinical and Experimental Medicine. Vol. 5, No. 2, 2017, pp. 46-49. doi: 10.11648/j.ajcem.20170502.14
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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