American Journal of Clinical and Experimental Medicine

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The Association Between Interleukin-10 Gene Promoter Polymorphism and Insulin Resistance in Type 2 Diabetes Mellitus

Received: 27 April 2016    Accepted: 16 May 2016    Published: 28 May 2016
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Abstract

Insulin resistance is a major characteristic of type 2 diabetes (T2DM). Inflammation plays an important role in increased insulin resistance, but the underlying mechanism remains unclear. Interleukin-10 (IL-10) is an anti-inflammatory cytokine with lower circulating levels in T2DM. We aimed to examine the association between IL-10 and insulin resistance, and to evaluate IL-10 gene promoter single nucleotide polymorphism (SNP) at position -592 C/A and serum IL-10 level as risk factors for insulin resistance and T2DM. This study was carried out on 200 subjects divided into 2 groups: 110 patients with T2DM (group I), and 90 healthy subjects served as controls (group II). All participants were investigated for; fasting and 2 hour post prandial blood glucose, serum lipids, glycated hemoglobin (HbA1c), serum IL-10 and fasting serum insulin. HOMA-IR was used for assessment of insulin resistance and β cell activity. Genotyping of -592 C/A (rs1800872) SNP of IL-10 gene promoter and genotype frequencies were analyzed using the polymerase chain reaction–restriction fragment length polymorphism technique (PCR-RFLP). The results of the present study showed significant statistical decrease in serum IL-10 levels in group I compared to group II. A significant negative correlation was found between serum IL-10 and HOMA-IR. Significant differences were observed for -592 C/A genotype distributions between both groups with increased frequency of the AA genotype in diabetic patients and increased frequency of CC genotype in controls. AA genotypes of -592 C/A was found to be a genetic risk factor for T2DM. Our results show that IL-10 has a positive association with insulin sensitivity, and SNP-592 C/A of IL-10 gene promoter and its serum level can contribute to susceptibility to insulin resistance and T2DM.

DOI 10.11648/j.ajcem.20160403.18
Published in American Journal of Clinical and Experimental Medicine (Volume 4, Issue 3, May 2016)
Page(s) 81-87
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Type 2 Diabetes Mellitus, IL-10, Gene Polymorphism

References
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Author Information
  • Medical Phsiyology Department, Faculty of Medicine - Menoufia University, Sheben AL-Kom, Egypt

  • Medical Biochemistry Department, Faculty of medicine - Menoufia University, Sheben AL-Kom, Egypt

  • Medical Biochemistry Department, Faculty of medicine - Menoufia University, Sheben AL-Kom, Egypt

  • Internal Medicine Department, Faculty of Medicine-Menoufia University, Sheben AL-Kom, Egypt

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    Sally Said Donia, Eman Masoud Abd El Gayed, Sally Mohamed El-Hefnawy, Ahmed Ragheb. (2016). The Association Between Interleukin-10 Gene Promoter Polymorphism and Insulin Resistance in Type 2 Diabetes Mellitus. American Journal of Clinical and Experimental Medicine, 4(3), 81-87. https://doi.org/10.11648/j.ajcem.20160403.18

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    Sally Said Donia; Eman Masoud Abd El Gayed; Sally Mohamed El-Hefnawy; Ahmed Ragheb. The Association Between Interleukin-10 Gene Promoter Polymorphism and Insulin Resistance in Type 2 Diabetes Mellitus. Am. J. Clin. Exp. Med. 2016, 4(3), 81-87. doi: 10.11648/j.ajcem.20160403.18

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    AMA Style

    Sally Said Donia, Eman Masoud Abd El Gayed, Sally Mohamed El-Hefnawy, Ahmed Ragheb. The Association Between Interleukin-10 Gene Promoter Polymorphism and Insulin Resistance in Type 2 Diabetes Mellitus. Am J Clin Exp Med. 2016;4(3):81-87. doi: 10.11648/j.ajcem.20160403.18

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  • @article{10.11648/j.ajcem.20160403.18,
      author = {Sally Said Donia and Eman Masoud Abd El Gayed and Sally Mohamed El-Hefnawy and Ahmed Ragheb},
      title = {The Association Between Interleukin-10 Gene Promoter Polymorphism and Insulin Resistance in Type 2 Diabetes Mellitus},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {4},
      number = {3},
      pages = {81-87},
      doi = {10.11648/j.ajcem.20160403.18},
      url = {https://doi.org/10.11648/j.ajcem.20160403.18},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajcem.20160403.18},
      abstract = {Insulin resistance is a major characteristic of type 2 diabetes (T2DM). Inflammation plays an important role in increased insulin resistance, but the underlying mechanism remains unclear. Interleukin-10 (IL-10) is an anti-inflammatory cytokine with lower circulating levels in T2DM. We aimed to examine the association between IL-10 and insulin resistance, and to evaluate IL-10 gene promoter single nucleotide polymorphism (SNP) at position -592 C/A and serum IL-10 level as risk factors for insulin resistance and T2DM. This study was carried out on 200 subjects divided into 2 groups: 110 patients with T2DM (group I), and 90 healthy subjects served as controls (group II). All participants were investigated for; fasting and 2 hour post prandial blood glucose, serum lipids, glycated hemoglobin (HbA1c), serum IL-10 and fasting serum insulin. HOMA-IR was used for assessment of insulin resistance and β cell activity. Genotyping of -592 C/A (rs1800872) SNP of IL-10 gene promoter and genotype frequencies were analyzed using the polymerase chain reaction–restriction fragment length polymorphism technique (PCR-RFLP). The results of the present study showed significant statistical decrease in serum IL-10 levels in group I compared to group II. A significant negative correlation was found between serum IL-10 and HOMA-IR. Significant differences were observed for -592 C/A genotype distributions between both groups with increased frequency of the AA genotype in diabetic patients and increased frequency of CC genotype in controls. AA genotypes of -592 C/A was found to be a genetic risk factor for T2DM. Our results show that IL-10 has a positive association with insulin sensitivity, and SNP-592 C/A of IL-10 gene promoter and its serum level can contribute to susceptibility to insulin resistance and T2DM.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - The Association Between Interleukin-10 Gene Promoter Polymorphism and Insulin Resistance in Type 2 Diabetes Mellitus
    AU  - Sally Said Donia
    AU  - Eman Masoud Abd El Gayed
    AU  - Sally Mohamed El-Hefnawy
    AU  - Ahmed Ragheb
    Y1  - 2016/05/28
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ajcem.20160403.18
    DO  - 10.11648/j.ajcem.20160403.18
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 81
    EP  - 87
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20160403.18
    AB  - Insulin resistance is a major characteristic of type 2 diabetes (T2DM). Inflammation plays an important role in increased insulin resistance, but the underlying mechanism remains unclear. Interleukin-10 (IL-10) is an anti-inflammatory cytokine with lower circulating levels in T2DM. We aimed to examine the association between IL-10 and insulin resistance, and to evaluate IL-10 gene promoter single nucleotide polymorphism (SNP) at position -592 C/A and serum IL-10 level as risk factors for insulin resistance and T2DM. This study was carried out on 200 subjects divided into 2 groups: 110 patients with T2DM (group I), and 90 healthy subjects served as controls (group II). All participants were investigated for; fasting and 2 hour post prandial blood glucose, serum lipids, glycated hemoglobin (HbA1c), serum IL-10 and fasting serum insulin. HOMA-IR was used for assessment of insulin resistance and β cell activity. Genotyping of -592 C/A (rs1800872) SNP of IL-10 gene promoter and genotype frequencies were analyzed using the polymerase chain reaction–restriction fragment length polymorphism technique (PCR-RFLP). The results of the present study showed significant statistical decrease in serum IL-10 levels in group I compared to group II. A significant negative correlation was found between serum IL-10 and HOMA-IR. Significant differences were observed for -592 C/A genotype distributions between both groups with increased frequency of the AA genotype in diabetic patients and increased frequency of CC genotype in controls. AA genotypes of -592 C/A was found to be a genetic risk factor for T2DM. Our results show that IL-10 has a positive association with insulin sensitivity, and SNP-592 C/A of IL-10 gene promoter and its serum level can contribute to susceptibility to insulin resistance and T2DM.
    VL  - 4
    IS  - 3
    ER  - 

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