Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF)
American Journal of Clinical and Experimental Medicine
Volume 4, Issue 3, May 2016, Pages: 56-62
Received: Mar. 24, 2016; Accepted: Apr. 16, 2016; Published: May 11, 2016
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Authors
Lenni Indriani, Department of Dental Material, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia
Mochammad Dharmautama, Department of Prosthodontic, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia
Edy Machmud, Department of Prosthodontic, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia
Muhammad Natsir Djide, Department of Microbiology and biotechnology, Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia
Mochammad Hatta, Molecular Biology and Immunology Laboratory, Departement of Microbiology. Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
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Abstract
Background: This study is performed to determine the effect of roselle extract gel 10% toward the changing of MMP - 8 mRNA expression in Gingival Creviculer Fluid (GCF) in patient with gingivitis experience post acrylic crown insertion. Methods: This study is a pre and post test experimental research. Research subjects were 9 patients who experience gingivitis post acrylic crown insertion and divided in three groups (treatment with roselle gel, negative control, and positive control). GCF samples were taken with paper strips before and seven days after application of roselle extract gel 10%. The change of MMP-8 mRNA expression is tested using Real Time-Polymerase Chain Reaction (RT-PCR) method. The results then statistically tested using paired T test to see the effect of rosella extract toward MMP-8 activity. Result: The MMP - 8 mRNA expression in gingivitis post acrylic crown insertion crown in the experimental group is significantly decreased after application of roselle extract gel 10%, which also seen in the positive control group, but no changes observed in the negative control group. Conclusions: Roselle extract gel 10% is proven to be effective in reducing the activity of MMP - 8 in GCF.
Keywords
Roselle Extract Gel, MMP - 8, Gingivitis, Acrylic Crown
To cite this article
Lenni Indriani, Mochammad Dharmautama, Edy Machmud, Muhammad Natsir Djide, Mochammad Hatta, Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF), American Journal of Clinical and Experimental Medicine. Vol. 4, No. 3, 2016, pp. 56-62. doi: 10.11648/j.ajcem.20160403.14
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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