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Detection of Helicobacter pylori and Human Papillomavirus in Peroperative Tissue Biopsies Collected from Malignancies in Oropharyngeal Area
American Journal of Clinical and Experimental Medicine
Volume 3, Issue 6, November 2015, Pages: 364-367
Received: Dec. 24, 2015; Published: Dec. 30, 2015
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Authors
Emil Pavlik, Department of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic; Department of Medical Biochemistry and Laboratory Medicine, First Faculty of Medicine, Charles University in Prague and General Faculty Hospital, Prague, Czech Republic; Department of Medical Disciplines and Population Protection, Faculty of Biomedical Engineering, Czech Technical University in Prague, Prague, Czech Republic
Eva Nartova, Department of Otorhinolaryngology, Third Faculty of Medicine, Charles University in Prague and Faculty Hospital Kralovske Vinohrady, Prague, Czech Republic
Jaromir Astl, Department of Otorhinolaryngology, Third Faculty of Medicine, Charles University in Prague and Military Faculty Hospital, Prague, Czech Republic
Barbora Drnkova, Department of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic; Department of Medical Disciplines and Population Protection, Faculty of Biomedical Engineering, Czech Technical University in Prague, Prague, Czech Republic
Petr Lukes, Department of Otorhinolaryngology, Head and Neck Surgery, First Faculty of Medicine, Charles University in Prague and Motol Faculty Hospital, Prague, Czech Republic
Bela Potuznikova, Department of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
Rami Katra, Department of Otolaryngology, Second Faculty of Medicine, Charles University in Prague and Motol Faculty Hospital, Prague, Czech Republic
Jaroslav Kraus, Ear, Nose and Throat Ward, Rudolph and Stephanie Hospital Benesov, Benesov, Czech Republic
Ivan Sterzl, Institute for Endocrinology, Prague, Czech Republic
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Abstract
Helicobacter pylori has been reported as pathogen of human GIT. It is associated with type B gastritis and peptic ulcers. Bacterium´s relationship to cancer has also been declared and H. pylori considered cancer-inductor. A number of studies documented H. pylori residence in oropharynx, generating hypotheses on participation in development of cancer in oropharyngeal area. Human papillomaviruses are DNA viruses colonizing skin and mucoid membranes of the host. Their oncogenic potential, especially in genitourinary system, has been confirmed. High-risk type HPV16 (group A9) is frequently reported as cancer-inductor in oropharyngeal area. The aim of this study is to contribute to discussions on induction of malignancies in oropharyngeal area, providing comparison of incidence of one bacterial and one viral pathogen in the cells and tissues of oropharyngeal neoplasia. Using real-time PCR-based tests, we investigated 70 tissue specimens collected during cancer surgery for detection of bacterial DNA of Helicobacter pylori and viral DNA of High risk HPV (groups A9, A7 and A5/6). Results: Helicobacter pylori DNA was detected in 60 samples (85.7%), while DNA of HPV only in 42 (60%). If focused on HPV-16 as proposed cancer inductor, it was detected in 34 samples (48.5%) only. No DNA of respective agents was detected in 7 samples (10%). There were 21 Helicobacter sole pathogen detections compared with only 3 of HPV. Conclusions: There is no doubt, Helicobacter pylori is a long-term resident in oropharynx and tonsils. This residence most likely influences functions of immune system, so that a newly entering contributor could switch-on the process resulting in cancer development. This could support high incidence of common detection of HPV and Helicobacter pylori in 39 samples (55.7%).
Keywords
Helicobacter pylori, Human Papillomavirus, Real-Time PCR, Cancer, Oropharynx, Genotype
To cite this article
Emil Pavlik, Eva Nartova, Jaromir Astl, Barbora Drnkova, Petr Lukes, Bela Potuznikova, Rami Katra, Jaroslav Kraus, Ivan Sterzl, Detection of Helicobacter pylori and Human Papillomavirus in Peroperative Tissue Biopsies Collected from Malignancies in Oropharyngeal Area, American Journal of Clinical and Experimental Medicine. Vol. 3, No. 6, 2015, pp. 364-367. doi: 10.11648/j.ajcem.20150306.17
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