Prevalence of obesity is increasing worldwide. One of the major risk factors for obesity is consumption of high fat diet (HFD). Gastrointestinal tract (GIT) is the first organ where HFD comes in contact with the body. But, the effect of HFD on the GIT especially the GIT barrier is not investigated properly. M cells are present in the Follicle associated epithelium (FAE) of Peyer’s patches (PP) of GIT and are important component of intestinal barrier. Healthy and adequate number of M cells is important for an effective intestinal barrier. Intestinal tight junction protein Claudin 4, situated in between the enterocytes of Peyer’s patches (PP), regulates the permeability through the intestinal mucosa. Reduced Claudin 4 is responsible for increased paracellular transport of antigenic materials. Calprotectin is an inflammatory marker secreted by neutrophils during inflammation. Its level is considered specific for intestinal inflammation. The objectives of this study were to investigate the expressions of M cells and Claudin 4 in the PP and to determine the fecal calprotectin level (FCP) of male Wistar rats fed HFD. Four weeks old, twenty male Wistar rats were divided into chow (n=10) and HFD (n=10) groups. After 6 weeks of consuming the respective diets, stool and GIT segments containing PP were collected. After tissue processing, tissues were sectioned into 3 micrometer thickness and were taken on poly-L-lysine coated glass slides. Immunohistochemical staining was done by rat M cell specific CK-8 antibody and anti-Claudin 4 antibody. Scoring was done to calculate the average number of M cells and Claudin 4 in the PP of both groups under light microscope. FCP were measured using a commercial enzyme linked immunoassay (ELISA) kit. Statistical analysis was done by chi-square test and independent T-test. Data are presented as mean ± SD. A p value <0.05 was considered as significant. The number of M cells in the PP was significantly higher in HFD group as compared to the control (p = 0.004). The expression of Claudin 4 in the PP was significantly decreased in HFD group as compared to the control (p = 0.018). The fecal calprotectin level in HFD group was significantly higher compared to the control (p = 0.016). HFD consumption for 6 weeks leads to a higher number of M cell and reduced the expression of Claudin 4 in the intestinal Peyer’s patches of male Wistar rats which might be due to GI inflammation.
| Published in | American Journal of Clinical and Experimental Medicine (Volume 3, Issue 5) |
| DOI | 10.11648/j.ajcem.20150305.25 |
| Page(s) | 283-287 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
High Fat Diet, M Cells, Claudin 4, Intestinal Barrier, Peyer’s Patches, Fecal Calprotectin
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APA Style
Auni Aqilah Zainal Abidin, Effat Omar, Mohammed Nasimul Islam, Jesmine Khan. (2015). High Fat Diet Alters the Expression of M Cells and Claudin 4 in the Peyer’s Patches of Rats. American Journal of Clinical and Experimental Medicine, 3(5), 283-287. https://doi.org/10.11648/j.ajcem.20150305.25
ACS Style
Auni Aqilah Zainal Abidin; Effat Omar; Mohammed Nasimul Islam; Jesmine Khan. High Fat Diet Alters the Expression of M Cells and Claudin 4 in the Peyer’s Patches of Rats. Am. J. Clin. Exp. Med. 2015, 3(5), 283-287. doi: 10.11648/j.ajcem.20150305.25
AMA Style
Auni Aqilah Zainal Abidin, Effat Omar, Mohammed Nasimul Islam, Jesmine Khan. High Fat Diet Alters the Expression of M Cells and Claudin 4 in the Peyer’s Patches of Rats. Am J Clin Exp Med. 2015;3(5):283-287. doi: 10.11648/j.ajcem.20150305.25
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Download@article{10.11648/j.ajcem.20150305.25,
author = {Auni Aqilah Zainal Abidin and Effat Omar and Mohammed Nasimul Islam and Jesmine Khan},
title = {High Fat Diet Alters the Expression of M Cells and Claudin 4 in the Peyer’s Patches of Rats},
journal = {American Journal of Clinical and Experimental Medicine},
volume = {3},
number = {5},
pages = {283-287},
doi = {10.11648/j.ajcem.20150305.25},
url = {https://doi.org/10.11648/j.ajcem.20150305.25},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150305.25},
abstract = {Prevalence of obesity is increasing worldwide. One of the major risk factors for obesity is consumption of high fat diet (HFD). Gastrointestinal tract (GIT) is the first organ where HFD comes in contact with the body. But, the effect of HFD on the GIT especially the GIT barrier is not investigated properly. M cells are present in the Follicle associated epithelium (FAE) of Peyer’s patches (PP) of GIT and are important component of intestinal barrier. Healthy and adequate number of M cells is important for an effective intestinal barrier. Intestinal tight junction protein Claudin 4, situated in between the enterocytes of Peyer’s patches (PP), regulates the permeability through the intestinal mucosa. Reduced Claudin 4 is responsible for increased paracellular transport of antigenic materials. Calprotectin is an inflammatory marker secreted by neutrophils during inflammation. Its level is considered specific for intestinal inflammation. The objectives of this study were to investigate the expressions of M cells and Claudin 4 in the PP and to determine the fecal calprotectin level (FCP) of male Wistar rats fed HFD. Four weeks old, twenty male Wistar rats were divided into chow (n=10) and HFD (n=10) groups. After 6 weeks of consuming the respective diets, stool and GIT segments containing PP were collected. After tissue processing, tissues were sectioned into 3 micrometer thickness and were taken on poly-L-lysine coated glass slides. Immunohistochemical staining was done by rat M cell specific CK-8 antibody and anti-Claudin 4 antibody. Scoring was done to calculate the average number of M cells and Claudin 4 in the PP of both groups under light microscope. FCP were measured using a commercial enzyme linked immunoassay (ELISA) kit. Statistical analysis was done by chi-square test and independent T-test. Data are presented as mean ± SD. A p value <0.05 was considered as significant. The number of M cells in the PP was significantly higher in HFD group as compared to the control (p = 0.004). The expression of Claudin 4 in the PP was significantly decreased in HFD group as compared to the control (p = 0.018). The fecal calprotectin level in HFD group was significantly higher compared to the control (p = 0.016). HFD consumption for 6 weeks leads to a higher number of M cell and reduced the expression of Claudin 4 in the intestinal Peyer’s patches of male Wistar rats which might be due to GI inflammation.},
year = {2015}
}
TY - JOUR T1 - High Fat Diet Alters the Expression of M Cells and Claudin 4 in the Peyer’s Patches of Rats AU - Auni Aqilah Zainal Abidin AU - Effat Omar AU - Mohammed Nasimul Islam AU - Jesmine Khan Y1 - 2015/11/26 PY - 2015 N1 - https://doi.org/10.11648/j.ajcem.20150305.25 DO - 10.11648/j.ajcem.20150305.25 T2 - American Journal of Clinical and Experimental Medicine JF - American Journal of Clinical and Experimental Medicine JO - American Journal of Clinical and Experimental Medicine SP - 283 EP - 287 PB - Science Publishing Group SN - 2330-8133 UR - https://doi.org/10.11648/j.ajcem.20150305.25 AB - Prevalence of obesity is increasing worldwide. One of the major risk factors for obesity is consumption of high fat diet (HFD). Gastrointestinal tract (GIT) is the first organ where HFD comes in contact with the body. But, the effect of HFD on the GIT especially the GIT barrier is not investigated properly. M cells are present in the Follicle associated epithelium (FAE) of Peyer’s patches (PP) of GIT and are important component of intestinal barrier. Healthy and adequate number of M cells is important for an effective intestinal barrier. Intestinal tight junction protein Claudin 4, situated in between the enterocytes of Peyer’s patches (PP), regulates the permeability through the intestinal mucosa. Reduced Claudin 4 is responsible for increased paracellular transport of antigenic materials. Calprotectin is an inflammatory marker secreted by neutrophils during inflammation. Its level is considered specific for intestinal inflammation. The objectives of this study were to investigate the expressions of M cells and Claudin 4 in the PP and to determine the fecal calprotectin level (FCP) of male Wistar rats fed HFD. Four weeks old, twenty male Wistar rats were divided into chow (n=10) and HFD (n=10) groups. After 6 weeks of consuming the respective diets, stool and GIT segments containing PP were collected. After tissue processing, tissues were sectioned into 3 micrometer thickness and were taken on poly-L-lysine coated glass slides. Immunohistochemical staining was done by rat M cell specific CK-8 antibody and anti-Claudin 4 antibody. Scoring was done to calculate the average number of M cells and Claudin 4 in the PP of both groups under light microscope. FCP were measured using a commercial enzyme linked immunoassay (ELISA) kit. Statistical analysis was done by chi-square test and independent T-test. Data are presented as mean ± SD. A p value <0.05 was considered as significant. The number of M cells in the PP was significantly higher in HFD group as compared to the control (p = 0.004). The expression of Claudin 4 in the PP was significantly decreased in HFD group as compared to the control (p = 0.018). The fecal calprotectin level in HFD group was significantly higher compared to the control (p = 0.016). HFD consumption for 6 weeks leads to a higher number of M cell and reduced the expression of Claudin 4 in the intestinal Peyer’s patches of male Wistar rats which might be due to GI inflammation. VL - 3 IS - 5 ER -
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