Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease

Redento Mora; Diana Binanti; Nicoletta Mora; Eleonora Fantinato; Valentina Ferrante; Luisella Pedrotti; Pietro Riccaboni

doi:doi:10.11648/j.ajcem.20150304.18

Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease

Redento Mora, Diana Binanti, Nicoletta Mora, Eleonora Fantinato, Valentina Ferrante, Luisella Pedrotti, Pietro Riccaboni

Published in American Journal of Clinical and Experimental Medicine (Volume 3, Issue 4)

Received: 25 May 2015 Accepted: 9 June 2015 Published: 25 June 2015

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Abstract

Background: MMP-2 and MMP-9 are proteolytic enzymes involved in the remodeling of extracellular matrix and regulated in their function by a family of proteins called TIMPs. MMP-2 and MMP-9 and TIMPs are secreted in joints by both synovial cells and chondrocytes, and are implicated in osteoarthritis onset and progression. The aim of this study was to investigate the localization of MMP-2 and TIMPs expression and their relationship in the synovial membrane of equine fetlock (metacarpo-phalangeal joint), which is the joint most frequently affected by osteoarthritis due to his high weight-bearing impact and shearing forces. Method: This study analyzed the immunohistochemical expression of MMP-2 and tissue inhibitors of MMP (TIMPs) in synovial membrane sections from 40 equine metacarpo-phalangeal joints affected by osteoarthritis in order to better assess their role in the pathogenesis of joint-structure alterations. Immunohistochemical study was performed using the Avidin-Biotin-Peroxidase complex method, and the percentage of labeled cells was assessed semi-quantitatively. Data obtained were statistically revised through the Spearman's rank correlation coefficient. Results: MMP-2 and TIMPs were expressed by type-B synoviocytes and endothelial cells. MMP-2 expression was not significantly associated to the macroscopic findings while a linear correlation between MMP-2 and TIMPs expression was observed. Conclusion: These data suggest that in degenerative joint disease the synovial expression of TIMPs may contribute to the regulation of MMP-2 expression in order to reach a new dynamic balance and that MMP-2 plays an important pro-homeostatic role in physiological and pathological equine synovial membrane.

Published in	American Journal of Clinical and Experimental Medicine (Volume 3, Issue 4)
DOI	10.11648/j.ajcem.20150304.18
Page(s)	172-177
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

MMPs, TIMPs, Fetlock, Horse, Immunohistochemistry

References

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APA Style

Redento Mora, Diana Binanti, Nicoletta Mora, Eleonora Fantinato, Valentina Ferrante, et al. (2015). Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease. American Journal of Clinical and Experimental Medicine, 3(4), 172-177. https://doi.org/10.11648/j.ajcem.20150304.18

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ACS Style

Redento Mora; Diana Binanti; Nicoletta Mora; Eleonora Fantinato; Valentina Ferrante, et al. Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease. Am. J. Clin. Exp. Med. 2015, 3(4), 172-177. doi: 10.11648/j.ajcem.20150304.18

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AMA Style

Redento Mora, Diana Binanti, Nicoletta Mora, Eleonora Fantinato, Valentina Ferrante, et al. Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease. Am J Clin Exp Med. 2015;3(4):172-177. doi: 10.11648/j.ajcem.20150304.18

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@article{10.11648/j.ajcem.20150304.18,
  author = {Redento Mora and Diana Binanti and Nicoletta Mora and Eleonora Fantinato and Valentina Ferrante and Luisella Pedrotti and Pietro Riccaboni},
  title = {Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {3},
  number = {4},
  pages = {172-177},
  doi = {10.11648/j.ajcem.20150304.18},
  url = {https://doi.org/10.11648/j.ajcem.20150304.18},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150304.18},
  abstract = {Background: MMP-2 and MMP-9 are proteolytic enzymes involved in the remodeling of extracellular matrix and regulated in their function by a family of proteins called TIMPs. MMP-2 and MMP-9 and TIMPs are secreted in joints by both synovial cells and chondrocytes, and are implicated in osteoarthritis onset and progression. The aim of this study was to investigate the localization of MMP-2 and TIMPs expression and their relationship in the synovial membrane of equine fetlock (metacarpo-phalangeal joint), which is the joint most frequently affected by osteoarthritis due to his high weight-bearing impact and shearing forces. Method: This study analyzed the immunohistochemical expression of MMP-2 and tissue inhibitors of MMP (TIMPs) in synovial membrane sections from 40 equine metacarpo-phalangeal joints affected by osteoarthritis in order to better assess their role in the pathogenesis of joint-structure alterations. Immunohistochemical study was performed using the Avidin-Biotin-Peroxidase complex method, and the percentage of labeled cells was assessed semi-quantitatively. Data obtained were statistically revised through the Spearman's rank correlation coefficient. Results: MMP-2 and TIMPs were expressed by type-B synoviocytes and endothelial cells. MMP-2 expression was not significantly associated to the macroscopic findings while a linear correlation between MMP-2 and TIMPs expression was observed. Conclusion: These data suggest that in degenerative joint disease the synovial expression of TIMPs may contribute to the regulation of MMP-2 expression in order to reach a new dynamic balance and that MMP-2 plays an important pro-homeostatic role in physiological and pathological equine synovial membrane.},
 year = {2015}
}

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TY  - JOUR
T1  - Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease
AU  - Redento Mora
AU  - Diana Binanti
AU  - Nicoletta Mora
AU  - Eleonora Fantinato
AU  - Valentina Ferrante
AU  - Luisella Pedrotti
AU  - Pietro Riccaboni
Y1  - 2015/06/25
PY  - 2015
N1  - https://doi.org/10.11648/j.ajcem.20150304.18
DO  - 10.11648/j.ajcem.20150304.18
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 172
EP  - 177
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20150304.18
AB  - Background: MMP-2 and MMP-9 are proteolytic enzymes involved in the remodeling of extracellular matrix and regulated in their function by a family of proteins called TIMPs. MMP-2 and MMP-9 and TIMPs are secreted in joints by both synovial cells and chondrocytes, and are implicated in osteoarthritis onset and progression. The aim of this study was to investigate the localization of MMP-2 and TIMPs expression and their relationship in the synovial membrane of equine fetlock (metacarpo-phalangeal joint), which is the joint most frequently affected by osteoarthritis due to his high weight-bearing impact and shearing forces. Method: This study analyzed the immunohistochemical expression of MMP-2 and tissue inhibitors of MMP (TIMPs) in synovial membrane sections from 40 equine metacarpo-phalangeal joints affected by osteoarthritis in order to better assess their role in the pathogenesis of joint-structure alterations. Immunohistochemical study was performed using the Avidin-Biotin-Peroxidase complex method, and the percentage of labeled cells was assessed semi-quantitatively. Data obtained were statistically revised through the Spearman's rank correlation coefficient. Results: MMP-2 and TIMPs were expressed by type-B synoviocytes and endothelial cells. MMP-2 expression was not significantly associated to the macroscopic findings while a linear correlation between MMP-2 and TIMPs expression was observed. Conclusion: These data suggest that in degenerative joint disease the synovial expression of TIMPs may contribute to the regulation of MMP-2 expression in order to reach a new dynamic balance and that MMP-2 plays an important pro-homeostatic role in physiological and pathological equine synovial membrane.
VL  - 3
IS  - 4
ER  -

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Author Information

Redento Mora

Orthopaedic Section
Diana Binanti

Department of Veterinary Science and Public Health, University of Milan, Veterinary University Hospital, School of Veterinary Medicine, Lodi, Italy
Nicoletta Mora

Department of Veterinary Science and Public Health, University of Milan, Veterinary University Hospital, School of Veterinary Medicine, Lodi, Italy
Eleonora Fantinato

Department of Veterinary Science and Public Health, University of Milan, Veterinary University Hospital, School of Veterinary Medicine, Lodi, Italy
Valentina Ferrante

Department of Veterinary Science and Public Health, University of Milan, Veterinary University Hospital, School of Veterinary Medicine, Lodi, Italy
Luisella Pedrotti

Orthopaedic Section
Pietro Riccaboni

Department of Veterinary Science and Public Health, University of Milan, Veterinary University Hospital, School of Veterinary Medicine, Lodi, Italy

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Plain Text BibTeX RIS

APA Style

Redento Mora, Diana Binanti, Nicoletta Mora, Eleonora Fantinato, Valentina Ferrante, et al. (2015). Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease. American Journal of Clinical and Experimental Medicine, 3(4), 172-177. https://doi.org/10.11648/j.ajcem.20150304.18

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ACS Style

Redento Mora; Diana Binanti; Nicoletta Mora; Eleonora Fantinato; Valentina Ferrante, et al. Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease. Am. J. Clin. Exp. Med. 2015, 3(4), 172-177. doi: 10.11648/j.ajcem.20150304.18

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AMA Style

Redento Mora, Diana Binanti, Nicoletta Mora, Eleonora Fantinato, Valentina Ferrante, et al. Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease. Am J Clin Exp Med. 2015;3(4):172-177. doi: 10.11648/j.ajcem.20150304.18

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@article{10.11648/j.ajcem.20150304.18,
  author = {Redento Mora and Diana Binanti and Nicoletta Mora and Eleonora Fantinato and Valentina Ferrante and Luisella Pedrotti and Pietro Riccaboni},
  title = {Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {3},
  number = {4},
  pages = {172-177},
  doi = {10.11648/j.ajcem.20150304.18},
  url = {https://doi.org/10.11648/j.ajcem.20150304.18},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150304.18},
  abstract = {Background: MMP-2 and MMP-9 are proteolytic enzymes involved in the remodeling of extracellular matrix and regulated in their function by a family of proteins called TIMPs. MMP-2 and MMP-9 and TIMPs are secreted in joints by both synovial cells and chondrocytes, and are implicated in osteoarthritis onset and progression. The aim of this study was to investigate the localization of MMP-2 and TIMPs expression and their relationship in the synovial membrane of equine fetlock (metacarpo-phalangeal joint), which is the joint most frequently affected by osteoarthritis due to his high weight-bearing impact and shearing forces. Method: This study analyzed the immunohistochemical expression of MMP-2 and tissue inhibitors of MMP (TIMPs) in synovial membrane sections from 40 equine metacarpo-phalangeal joints affected by osteoarthritis in order to better assess their role in the pathogenesis of joint-structure alterations. Immunohistochemical study was performed using the Avidin-Biotin-Peroxidase complex method, and the percentage of labeled cells was assessed semi-quantitatively. Data obtained were statistically revised through the Spearman's rank correlation coefficient. Results: MMP-2 and TIMPs were expressed by type-B synoviocytes and endothelial cells. MMP-2 expression was not significantly associated to the macroscopic findings while a linear correlation between MMP-2 and TIMPs expression was observed. Conclusion: These data suggest that in degenerative joint disease the synovial expression of TIMPs may contribute to the regulation of MMP-2 expression in order to reach a new dynamic balance and that MMP-2 plays an important pro-homeostatic role in physiological and pathological equine synovial membrane.},
 year = {2015}
}

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TY  - JOUR
T1  - Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease
AU  - Redento Mora
AU  - Diana Binanti
AU  - Nicoletta Mora
AU  - Eleonora Fantinato
AU  - Valentina Ferrante
AU  - Luisella Pedrotti
AU  - Pietro Riccaboni
Y1  - 2015/06/25
PY  - 2015
N1  - https://doi.org/10.11648/j.ajcem.20150304.18
DO  - 10.11648/j.ajcem.20150304.18
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 172
EP  - 177
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20150304.18
AB  - Background: MMP-2 and MMP-9 are proteolytic enzymes involved in the remodeling of extracellular matrix and regulated in their function by a family of proteins called TIMPs. MMP-2 and MMP-9 and TIMPs are secreted in joints by both synovial cells and chondrocytes, and are implicated in osteoarthritis onset and progression. The aim of this study was to investigate the localization of MMP-2 and TIMPs expression and their relationship in the synovial membrane of equine fetlock (metacarpo-phalangeal joint), which is the joint most frequently affected by osteoarthritis due to his high weight-bearing impact and shearing forces. Method: This study analyzed the immunohistochemical expression of MMP-2 and tissue inhibitors of MMP (TIMPs) in synovial membrane sections from 40 equine metacarpo-phalangeal joints affected by osteoarthritis in order to better assess their role in the pathogenesis of joint-structure alterations. Immunohistochemical study was performed using the Avidin-Biotin-Peroxidase complex method, and the percentage of labeled cells was assessed semi-quantitatively. Data obtained were statistically revised through the Spearman's rank correlation coefficient. Results: MMP-2 and TIMPs were expressed by type-B synoviocytes and endothelial cells. MMP-2 expression was not significantly associated to the macroscopic findings while a linear correlation between MMP-2 and TIMPs expression was observed. Conclusion: These data suggest that in degenerative joint disease the synovial expression of TIMPs may contribute to the regulation of MMP-2 expression in order to reach a new dynamic balance and that MMP-2 plays an important pro-homeostatic role in physiological and pathological equine synovial membrane.
VL  - 3
IS  - 4
ER  -

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