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Monitoring Microbiological Response to Antituberculosis Therapy by Real-Time PCR
American Journal of Clinical and Experimental Medicine
Volume 3, Issue 1, January 2015, Pages: 1-5
Received: Oct. 19, 2014; Accepted: Nov. 6, 2014; Published: Jan. 19, 2015
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Authors
Yadi Yasir, Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar,Indonesia; Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Ressy Dwiyanti, Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar,Indonesia; Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Muhammad Sabir, Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar,Indonesia; Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Andini Febrianty, Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar,Indonesia; Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Ahmad Adhyka, Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar,Indonesia; Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Nur Indah Purnamasari, Department of Microbiology, Faculty of Medicine, Haluoleo University, Kendari, Indonesia
Muhammad Reza Primaguna, Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar,Indonesia; Department of Microbiology, Faculty of Medicine, Haluoleo University, Kendari, Indonesia
Nataniel Tandirogang, Department Microbiology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia
Masyhudi Amir, Department Microbiology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia
Syamsu Rijal, Department of Histology, Faculty of Medicine, Muslim University of Indonesia, Makassar, Indonesia
Rosdiana Natzir, Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
SutjiPratiwi Rahardjo, Department of Otorhinolaryngology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Mochammad Hatta, Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar,Indonesia
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Abstract
Background: M. tuberculosis grows slowly and requires several weeks to detect in clinical specimens using standard culture techniques. Messenger RNA is more rapidly destroyed in cells than rRNA or genomic DNA, assays that target mycobacterial mRNA better reflect mycobacterial viability. Therefore we evaluated performance of mRNA for monitoring response to antituberculosis therapy using Real-Time PCR. Methods: Sputum specimens from 29 tuberculosis patients confirmed by positive culture were included in this study. Sputum specimens were collected before therapy, at 1st week, 4th week, 8th week and 16th week after initiating antituberculosis therapy to evaluate acid-fast bacilli (AFB), culture and mRNA level. Results: All 29 specimens were positive for culture and mRNA before initiating tuberculosis chemotherapy. Within 29 samples confirmed by positive culture, only 22 (75.9%) patients were positive by AFB. After 8 and 16 weeks of therapy, 27 (93.1%) and 28 (96.5%) were negative by culture, 26 (89.6%) and 28 (96.5%) were negative results for mRNA, and 15 (51.7%) and 29 (100%) were negative results for AFB. Conclusion: Rapidly decline of mRNA level correlated with rapid culture clearance after anti-tuberculosis therapy.
Keywords
M. tuberculosis, mRNA, Real-Time PCR
To cite this article
Yadi Yasir, Ressy Dwiyanti, Muhammad Sabir, Andini Febrianty, Ahmad Adhyka, Nur Indah Purnamasari, Muhammad Reza Primaguna, Nataniel Tandirogang, Masyhudi Amir, Syamsu Rijal, Rosdiana Natzir, SutjiPratiwi Rahardjo, Mochammad Hatta, Monitoring Microbiological Response to Antituberculosis Therapy by Real-Time PCR, American Journal of Clinical and Experimental Medicine. Vol. 3, No. 1, 2015, pp. 1-5. doi: 10.11648/j.ajcem.20150301.11
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