Monitoring Microbiological Response to Antituberculosis Therapy by Real-Time PCR

Yadi Yasir; Ressy Dwiyanti; Muhammad Sabir; Andini Febrianty; Ahmad Adhyka; Nur Indah Purnamasari; Muhammad Reza Primaguna; Nataniel Tandirogang; Masyhudi Amir; Syamsu Rijal; Rosdiana Natzir; SutjiPratiwi Rahardjo; Mochammad Hatta

doi:doi:10.11648/j.ajcem.20150301.11

Monitoring Microbiological Response to Antituberculosis Therapy by Real-Time PCR

Yadi Yasir, Ressy Dwiyanti, Muhammad Sabir, Andini Febrianty, Ahmad Adhyka, Nur Indah Purnamasari, Muhammad Reza Primaguna, Nataniel Tandirogang, Masyhudi Amir, Syamsu Rijal, Rosdiana Natzir, SutjiPratiwi Rahardjo, Mochammad Hatta

Published in American Journal of Clinical and Experimental Medicine (Volume 3, Issue 1)

Received: 19 October 2014 Accepted: 6 November 2014 Published: 19 January 2015

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Abstract

Background: M. tuberculosis grows slowly and requires several weeks to detect in clinical specimens using standard culture techniques. Messenger RNA is more rapidly destroyed in cells than rRNA or genomic DNA, assays that target mycobacterial mRNA better reflect mycobacterial viability. Therefore we evaluated performance of mRNA for monitoring response to antituberculosis therapy using Real-Time PCR. Methods: Sputum specimens from 29 tuberculosis patients confirmed by positive culture were included in this study. Sputum specimens were collected before therapy, at 1st week, 4th week, 8th week and 16th week after initiating antituberculosis therapy to evaluate acid-fast bacilli (AFB), culture and mRNA level. Results: All 29 specimens were positive for culture and mRNA before initiating tuberculosis chemotherapy. Within 29 samples confirmed by positive culture, only 22 (75.9%) patients were positive by AFB. After 8 and 16 weeks of therapy, 27 (93.1%) and 28 (96.5%) were negative by culture, 26 (89.6%) and 28 (96.5%) were negative results for mRNA, and 15 (51.7%) and 29 (100%) were negative results for AFB. Conclusion: Rapidly decline of mRNA level correlated with rapid culture clearance after anti-tuberculosis therapy.

Published in	American Journal of Clinical and Experimental Medicine (Volume 3, Issue 1)
DOI	10.11648/j.ajcem.20150301.11
Page(s)	1-5
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

M. tuberculosis, mRNA, Real-Time PCR

References

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APA Style

Yadi Yasir, Ressy Dwiyanti, Muhammad Sabir, Andini Febrianty, Ahmad Adhyka, et al. (2015). Monitoring Microbiological Response to Antituberculosis Therapy by Real-Time PCR. American Journal of Clinical and Experimental Medicine, 3(1), 1-5. https://doi.org/10.11648/j.ajcem.20150301.11

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ACS Style

Yadi Yasir; Ressy Dwiyanti; Muhammad Sabir; Andini Febrianty; Ahmad Adhyka, et al. Monitoring Microbiological Response to Antituberculosis Therapy by Real-Time PCR. Am. J. Clin. Exp. Med. 2015, 3(1), 1-5. doi: 10.11648/j.ajcem.20150301.11

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AMA Style

Yadi Yasir, Ressy Dwiyanti, Muhammad Sabir, Andini Febrianty, Ahmad Adhyka, et al. Monitoring Microbiological Response to Antituberculosis Therapy by Real-Time PCR. Am J Clin Exp Med. 2015;3(1):1-5. doi: 10.11648/j.ajcem.20150301.11

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@article{10.11648/j.ajcem.20150301.11,
  author = {Yadi Yasir and Ressy Dwiyanti and Muhammad Sabir and Andini Febrianty and Ahmad Adhyka and Nur Indah Purnamasari and Muhammad Reza Primaguna and Nataniel Tandirogang and Masyhudi Amir and Syamsu Rijal and Rosdiana Natzir and SutjiPratiwi Rahardjo and Mochammad Hatta},
  title = {Monitoring Microbiological Response to Antituberculosis Therapy by Real-Time PCR},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {3},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.ajcem.20150301.11},
  url = {https://doi.org/10.11648/j.ajcem.20150301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150301.11},
  abstract = {Background: M. tuberculosis grows slowly and requires several weeks to detect in clinical specimens using standard culture techniques. Messenger RNA is more rapidly destroyed in cells than rRNA or genomic DNA, assays that target mycobacterial mRNA better reflect mycobacterial viability. Therefore we evaluated performance of mRNA for monitoring response to antituberculosis therapy using Real-Time PCR. Methods: Sputum specimens from 29 tuberculosis patients confirmed by positive culture were included in this study. Sputum specimens were collected before therapy, at 1st week, 4th week, 8th week and 16th week after initiating antituberculosis therapy to evaluate acid-fast bacilli (AFB), culture and mRNA level. Results: All 29 specimens were positive for culture and mRNA before initiating tuberculosis chemotherapy. Within 29 samples confirmed by positive culture, only 22 (75.9%) patients were positive by AFB. After 8 and 16 weeks of therapy, 27 (93.1%) and 28 (96.5%) were negative by culture, 26 (89.6%) and 28 (96.5%) were negative results for mRNA, and 15 (51.7%) and 29 (100%) were negative results for AFB. Conclusion: Rapidly decline of mRNA level correlated with rapid culture clearance after anti-tuberculosis therapy.},
 year = {2015}
}

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TY  - JOUR
T1  - Monitoring Microbiological Response to Antituberculosis Therapy by Real-Time PCR
AU  - Yadi Yasir
AU  - Ressy Dwiyanti
AU  - Muhammad Sabir
AU  - Andini Febrianty
AU  - Ahmad Adhyka
AU  - Nur Indah Purnamasari
AU  - Muhammad Reza Primaguna
AU  - Nataniel Tandirogang
AU  - Masyhudi Amir
AU  - Syamsu Rijal
AU  - Rosdiana Natzir
AU  - SutjiPratiwi Rahardjo
AU  - Mochammad Hatta
Y1  - 2015/01/19
PY  - 2015
N1  - https://doi.org/10.11648/j.ajcem.20150301.11
DO  - 10.11648/j.ajcem.20150301.11
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 1
EP  - 5
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20150301.11
AB  - Background: M. tuberculosis grows slowly and requires several weeks to detect in clinical specimens using standard culture techniques. Messenger RNA is more rapidly destroyed in cells than rRNA or genomic DNA, assays that target mycobacterial mRNA better reflect mycobacterial viability. Therefore we evaluated performance of mRNA for monitoring response to antituberculosis therapy using Real-Time PCR. Methods: Sputum specimens from 29 tuberculosis patients confirmed by positive culture were included in this study. Sputum specimens were collected before therapy, at 1st week, 4th week, 8th week and 16th week after initiating antituberculosis therapy to evaluate acid-fast bacilli (AFB), culture and mRNA level. Results: All 29 specimens were positive for culture and mRNA before initiating tuberculosis chemotherapy. Within 29 samples confirmed by positive culture, only 22 (75.9%) patients were positive by AFB. After 8 and 16 weeks of therapy, 27 (93.1%) and 28 (96.5%) were negative by culture, 26 (89.6%) and 28 (96.5%) were negative results for mRNA, and 15 (51.7%) and 29 (100%) were negative results for AFB. Conclusion: Rapidly decline of mRNA level correlated with rapid culture clearance after anti-tuberculosis therapy.
VL  - 3
IS  - 1
ER  -

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Author Information

Yadi Yasir

Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar,Indonesia; Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Ressy Dwiyanti

Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar,Indonesia; Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Muhammad Sabir

Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar,Indonesia; Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Andini Febrianty

Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar,Indonesia; Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Ahmad Adhyka

Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar,Indonesia; Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Nur Indah Purnamasari

Department of Microbiology, Faculty of Medicine, Haluoleo University, Kendari, Indonesia
Muhammad Reza Primaguna

Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar,Indonesia; Department of Microbiology, Faculty of Medicine, Haluoleo University, Kendari, Indonesia
Nataniel Tandirogang

Department Microbiology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia
Masyhudi Amir

Department Microbiology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia
Syamsu Rijal

Department of Histology, Faculty of Medicine, Muslim University of Indonesia, Makassar, Indonesia
Rosdiana Natzir

Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
SutjiPratiwi Rahardjo

Department of Otorhinolaryngology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Mochammad Hatta

Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar,Indonesia

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Plain Text BibTeX RIS

APA Style

Yadi Yasir, Ressy Dwiyanti, Muhammad Sabir, Andini Febrianty, Ahmad Adhyka, et al. (2015). Monitoring Microbiological Response to Antituberculosis Therapy by Real-Time PCR. American Journal of Clinical and Experimental Medicine, 3(1), 1-5. https://doi.org/10.11648/j.ajcem.20150301.11

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ACS Style

Yadi Yasir; Ressy Dwiyanti; Muhammad Sabir; Andini Febrianty; Ahmad Adhyka, et al. Monitoring Microbiological Response to Antituberculosis Therapy by Real-Time PCR. Am. J. Clin. Exp. Med. 2015, 3(1), 1-5. doi: 10.11648/j.ajcem.20150301.11

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AMA Style

Yadi Yasir, Ressy Dwiyanti, Muhammad Sabir, Andini Febrianty, Ahmad Adhyka, et al. Monitoring Microbiological Response to Antituberculosis Therapy by Real-Time PCR. Am J Clin Exp Med. 2015;3(1):1-5. doi: 10.11648/j.ajcem.20150301.11

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@article{10.11648/j.ajcem.20150301.11,
  author = {Yadi Yasir and Ressy Dwiyanti and Muhammad Sabir and Andini Febrianty and Ahmad Adhyka and Nur Indah Purnamasari and Muhammad Reza Primaguna and Nataniel Tandirogang and Masyhudi Amir and Syamsu Rijal and Rosdiana Natzir and SutjiPratiwi Rahardjo and Mochammad Hatta},
  title = {Monitoring Microbiological Response to Antituberculosis Therapy by Real-Time PCR},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {3},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.ajcem.20150301.11},
  url = {https://doi.org/10.11648/j.ajcem.20150301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150301.11},
  abstract = {Background: M. tuberculosis grows slowly and requires several weeks to detect in clinical specimens using standard culture techniques. Messenger RNA is more rapidly destroyed in cells than rRNA or genomic DNA, assays that target mycobacterial mRNA better reflect mycobacterial viability. Therefore we evaluated performance of mRNA for monitoring response to antituberculosis therapy using Real-Time PCR. Methods: Sputum specimens from 29 tuberculosis patients confirmed by positive culture were included in this study. Sputum specimens were collected before therapy, at 1st week, 4th week, 8th week and 16th week after initiating antituberculosis therapy to evaluate acid-fast bacilli (AFB), culture and mRNA level. Results: All 29 specimens were positive for culture and mRNA before initiating tuberculosis chemotherapy. Within 29 samples confirmed by positive culture, only 22 (75.9%) patients were positive by AFB. After 8 and 16 weeks of therapy, 27 (93.1%) and 28 (96.5%) were negative by culture, 26 (89.6%) and 28 (96.5%) were negative results for mRNA, and 15 (51.7%) and 29 (100%) were negative results for AFB. Conclusion: Rapidly decline of mRNA level correlated with rapid culture clearance after anti-tuberculosis therapy.},
 year = {2015}
}

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TY  - JOUR
T1  - Monitoring Microbiological Response to Antituberculosis Therapy by Real-Time PCR
AU  - Yadi Yasir
AU  - Ressy Dwiyanti
AU  - Muhammad Sabir
AU  - Andini Febrianty
AU  - Ahmad Adhyka
AU  - Nur Indah Purnamasari
AU  - Muhammad Reza Primaguna
AU  - Nataniel Tandirogang
AU  - Masyhudi Amir
AU  - Syamsu Rijal
AU  - Rosdiana Natzir
AU  - SutjiPratiwi Rahardjo
AU  - Mochammad Hatta
Y1  - 2015/01/19
PY  - 2015
N1  - https://doi.org/10.11648/j.ajcem.20150301.11
DO  - 10.11648/j.ajcem.20150301.11
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 1
EP  - 5
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20150301.11
AB  - Background: M. tuberculosis grows slowly and requires several weeks to detect in clinical specimens using standard culture techniques. Messenger RNA is more rapidly destroyed in cells than rRNA or genomic DNA, assays that target mycobacterial mRNA better reflect mycobacterial viability. Therefore we evaluated performance of mRNA for monitoring response to antituberculosis therapy using Real-Time PCR. Methods: Sputum specimens from 29 tuberculosis patients confirmed by positive culture were included in this study. Sputum specimens were collected before therapy, at 1st week, 4th week, 8th week and 16th week after initiating antituberculosis therapy to evaluate acid-fast bacilli (AFB), culture and mRNA level. Results: All 29 specimens were positive for culture and mRNA before initiating tuberculosis chemotherapy. Within 29 samples confirmed by positive culture, only 22 (75.9%) patients were positive by AFB. After 8 and 16 weeks of therapy, 27 (93.1%) and 28 (96.5%) were negative by culture, 26 (89.6%) and 28 (96.5%) were negative results for mRNA, and 15 (51.7%) and 29 (100%) were negative results for AFB. Conclusion: Rapidly decline of mRNA level correlated with rapid culture clearance after anti-tuberculosis therapy.
VL  - 3
IS  - 1
ER  -

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