American Journal of Clinical and Experimental Medicine

| Peer-Reviewed |

Nephro-Protective Effects of Curcumin, Rosemary and Propolis against Gentamicin Induced Toxicity in Guinea Pigs: Morphological and Biochemical Study

Received: 25 March 2014    Accepted: 10 April 2014    Published: 20 April 2014
Views:       Downloads:

Share This Article

Abstract

The kidney is a common target for toxic xenobiotics due to its capacity to extract and concentrate toxic substances by highly specialized cells and also, due to its large blood flow. Objective: The present work aimed to evaluate the effectiveness of different natural materials (curcumin, rosemary and propolis) against the histological and also biochemical alterations of gentamicin induced nephrotoxicity in guinea pigs. Materials and methods: 48 guinea pigs were used for this study and divided into 8 groups. The first 4 groups were control groups, the 5th group was the experimental and administered gentamicin at a dose of 100 mg/kg body wt for 10 days, and in the 6th , 7th , and 8th groups, gentamicin was co-administered with curcumin, rosemary, and propolis at the doses of 200 mg, 220 mg, and 100 mg/kg body wt respectively. The animals were sacrificed and the kidneys were dissected and specimens were obtained. The specimens were processed for light microscopic examinations. Blood samples were obtained for assessment of urea, creatinine and uric acid levels. Results: In gentamicin treated animals, there were structural changes. The proximal convoluted tubules showed degenerated epithelial lining with disruption of their brush borders and presence of epithelial debris inside their lumens. The renal corpuscle appeared with degeneration of the glomerulus and disrupted Bowman's capsule. The afferent arteriole showed thickening in its wall and degeneration of endothelial lining with extensive perivascular infiltration of inflammatory cells. Massive interstitial hemorrhage was seen. Also, the serum urea, creatinine, and uric acid were elevated. Co-administration of curcumin, rosemary, and propolis significantly improved the structural changes in the kidney and the blood urea, creatinine and uric acid were significantly declined. Conclusion: It can be concluded that, the gentamicin has adverse effects on the kidney. Different natural materials as curcumin, rosemary, and propolis were able to protect the kidney against these effects. So, the patients should be advised to take one of these materials while they are treated by gentamicin.

DOI 10.11648/j.ajcem.20140202.14
Published in American Journal of Clinical and Experimental Medicine (Volume 2, Issue 2, March 2014)
Page(s) 28-35
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Nephrotoxicity, Histology, Biochemical, Gentamicin, Curcumin, Rosemary, Propolis

References
[1] Salgado CM, Hernades FL and Novoa JM: Glandular nephrotoxicity of aminonucleosides. Toxicol App Pharmacol 2007;223: 86-98
[2] Choi JJ, Moffett BS, McDade and Palazzo DL: Altered gentamicin serum concentration in obese pediatric patients. Pediat Infect Dis J 2011;30: 347-349
[3] Kaloyanides GJ: Metabolic interactions between drugs and renal tubulo-interstitial cells: role in nephrotoxicity. Kidney Int 1991; 39: 531-540
[4] Balakumar P, Chakkarwar VA, Kumar V, Jain A, Reddy J and Singh M: Experimental models for nephropathy. J Renin Angiotensin Aldosterone System 2008;9: 189-95
[5] Mattew TH: Drug-induced renal disease. Med J Aust 1992;156: 724-728
[6] Ali BH: Agents ameliorating or augmenting experimental gentamicin nephrotoxicity. Some recent research. Food Chem Toxicol 2003;41: 1447-1452
[7] Rincon J, Romero M, Viera N, Pedreanea A and Mosquera J: Increased oxidative stress and apoptosis in acute puromycin aminonucleoside nephrosis. Int J Exp Pathol 2004;85(1): 25-33
[8] DeBroe ME, Giuliano RA and Verpooten GA: Aminoglycoside nephrotoxicity: mechanism and prevention. Adv Exp Med Biol 1989;252: 233-245
[9] Cuzzocrea S, Mazzon E, Dugo L Serraino I, Di Paola R, Britti D, De Sarro A, Pierpaoli S, Caputi A, Masini E, Salvemini D: A role for superoxide in gentamicin mediated nephropathy in rats. Eur J Pharmacol 2002; 450(1): 67-76
[10] Karahan I, Atessahin A, Yilmaz S, Ceribassi AO, Sakin F: Protective effect of lycopene on gentamicin-induced oxidative stress and nephrotoxicity in rats. Toxicol 2005;215: 192-204
[11] Heeba GH, and Abd-Elghany MI: Effect of combined administration of ginger (Zingiber Officinale Roscoe) and atorvastatin on the liver of rats. Phytomedicine 2010;17: 1076-81
[12] Ho C, Ferrara T, Chen Q, Rosen R and Huang M: Phytochemicals in teas and rosemary and their cancer preventive properties in: food phytochemicals for cancer prevention. American Chemical Society, Washington, DC, 1994, pp.2-19
[13] Joe B, Vijaykumar M, Lokesh BR: Biological properties of curcumin-cellular and molecular mechanisms of action. Crit Rev Food Sci Nutr 2004;44: 97-111
[14] Venkatesan N, Punithavathi D, Arumugan V: Curcumin prevents adriamycin nephrotoxicity in rats. Br J Pharmacol 2000;12: 231-234
[15] Tirkey N, Kaur G, Vij G and Chopra K: Curcumin a diferuloylmethane, attenuates cyclosporine induced renal dysfunction and oxidative stress in rat kidneys. J Biosc 2005; 22(2): 233-46
[16] Ruby AJ, Kuttan G, Babu KD and Kuttan R: Anti-tumor and anti-oxidant activity of natural curcuminoids. Cancer Lett 2005;94: 79-83
[17] Aruoma O, Halliwell B, Aeschbach R, Loligers J: Antioxidant and pro-oxidant properties of active rosemary constituents: carnosol and carnosic acid. Xenobiotica 1992;22: 257-268
[18] Altinier G, Sosa S, Aquino RP, Mencherini T, Della Loggia R, Tubaro A: Characterization of topical anti-inflammatory compounds in Rosmarinus Officinalis L. J Agric Food Chem 2007;55: 1718-1723
[19] Marquele FD, Di Mambro VM, Georgetti SR, Casagrande R, Valim YML and Fonseca MJV: Assessment of the antioxidant activities of Brazilian extracts of propolis alone and in topical pharmaceutical formulation. J Pharmacol Biomed Anal 2005;39: 455-462
[20] Li YJ, Lin JL, Yang CW and Yu CC: Acute renal failure induced by a Brazilian variety of propolis. Am J Kid Dis 2005;46(6): e125-9
[21] Gunduz C, Biray C, Kosova B, Yilmaz B, Eroglu Z, Sahin F, Omay SB and Cogulu O: Evaluation of Manisa propolis effect on leukemia cell line by telomerase activity. Leuk Res 2005;29(11): 1343-6
[22] Ozguner F, Bardak Y and Comlekci S: Protective effects of melatonin and caffeic acid phenethyl ester against retinal oxidative stress in long-term use of mobile phone. a comparative study. Mol Cell Biochem 2006;282(1-2): 83-88
[23] Amin A, and Hamza AA: Hepatoprotective effects of hibiscus, rosemarinus and salvia on azathioprine-induced toxicity in rats. Life Sciences 2005;77: 266-278
[24] El-Khayat Z, Ezzat AR, Arbid MS, Rasheed WI, Elias TR: Potential effects of bee honey and propolis against the toxicity of ochratoxin a in rats. Macedonian J Med Scie 2009;2(4): 1-8
[25] Elfarra AA, Duescher RJ, Sausen PJ, OʼHara TM and Cooley AJ: Methimazole protection of rats against gentamicin-induced nephrotoxicity. Can J Physiol Pharmacol 1994;72: 1238-1244
[26] Kumar KV, Naidu MUR, Shifow AA and Ratnakar KS: Probucol Protects against gentamicin-induced nephrotoxicity in rats. J Pharmacol 2000;32: 108-113
[27] Ali BH, Al-Qarawi AA, Mahmoud OM and Hashad M: Influence of spironolactone treatment on gentamicin -induced nephrotoxicity in rats. Basic Clin Pharmacol Toxicol 2004;95: 20-23
[28] Chuang SE, Chen AL, Lin JK and Kuo ML: Inhibition by curcumin of diethylnitrosamine-induced hepatic hyperplasia, inflammation, cellular gene protection and cell-cycle related protein in rats . Food Chem Toxicol 2000;38: 991-995
[29] Ali BH, Al-Wabel N, Mahmoud O, Mousa HM, Hashad M: Curcumin has a palliative action on gentamicin-induced nephrotoxicity in rats. Fundamental and clin pharmacol 2005;19: 473-477
[30] Dorman HJ, Peltoketo A, Hiltunen R and Tikkanen MJ: Characterization of the antioxidants properties of de-odourised aqueous extracts from selected lamiaceae herbs. Food Chemistry 2003;83: 255-262
[31] Sakr SA and Lamfon HA: Protective effect of rosemary (Rosmarinus Officinalis) leaves extracts on carbon tetrachloride-induced nephrotoxicity in albino rats. Life science journal 2012;9(1): 779-785
[32] Ross MH, Reith EJ and Romrell LJ: Histology: A Text Atlas (2nded.). Baltimore. Williams &Wilkins,1989, pp.51-84
[33] Spencer K: Analytical reviews in clinical biochemistry: the estimation of creatinine. Ann Clin Biochem 1986;23: 1-25
[34] Banday AA, Farooq N, Priyamvada S, Yusufi ANK and Khan F: Time dependent effect of carbohydrate metabolism, brush border membrane and oxidative stress in rat kidney tissue. Life Science 2008;82: 450-459
[35] Padmini MP and Kumar JV: A histological study on gentamicin indued nephrotoxicity in experimental albino rats. J Dent Med Sci 2012; 1(1): 14-17
[36] Manikanadan R, Beulaja M, Thiagarajan R, Priyadarsini A, Saravanan R and Arumugam M: Ameliorative effects of curcumin against renal injuries mediated by inducible nitric oxide synthase and nuclear factor kappa B during gentamicin-induced toxicity in wister rats. Eur J Pharm 2011;670: 578-585
[37] Lakshmi BVS and Sudhakar M: Protective effect of zingiber officinalis on gentamicin induced nephrotoxicity in rats. Int J Pharmacol 2010;6: 58-62
[38] Souza VB, Oliveira RFL, Ferreira AAA and Araujo-junior RF: Renal alteration by aminoglycosides. Arq Med 2008:22(45): 131-135
[39] Ali BH and Basher AA: Effect of fish oil treatment on gentamicin nephrotoxicity in rats. Ann Nutr Metab 1994;38: 336-339
[40] Nale LP, More PR, More BK, Ghumare BC, Shendre SD and Mote CS: Protective effect of carica papaya l. seed extract in gentamicin induced hepatotoxicity and nephrotoxicity in rats. Int J Pharm Bio Sci 2012;3(3): 508-515
[41] Kang C, Lee H, Hah D, Heo JH, Kim CH, Kim E, and Kim JS: Protective effects of houttuynia cordata thunb on gentamicin induced oxidative stress and nephrotoxicity in rats. Toxicol Res 2013;29(1): 61-67
[42] Lakshmi BVS, Neelima N, Kasthuri N, Umarani V and Sudhakar M: Protective effect of bauhinia purpurea on gentamicin induced nephrotoxicity in rats. Indian J Pharma Sci 2009;71(5): 551-554
[43] Ullah N, Khan MA, Khan T and Ahmad W: Protective effect of gentamicin induced nephrotic damage in rabbit. Trop J Pharmaceut Res 2013;12(2): 215-219
[44] Alarifi S, Al-Doaiss A, Alkahtani S, Al-Farraj SA, Al-Eissa MS, Al-Dahmash B, Al-Yahya H And Mubarak M: Blood chemical changes and renal histological alternations induced by gentamicin in rats. Saudi J Biol Sci 2012;19: 103-110
[45] Nagai J, And Takano M: Molecular aspect of renal handling of aminoglycosides and strategies for preventing the nephrotoxicity. Drug metab pharmacokinet 2004;19(3): 159-70
[46] Whiting PH, and Brown PAS: The relationship between enzymuria and kidney enzyme activities in experimental gentamicin nephrotoxicity. Renal Failure 1996;18(6): 899-909
[47] Noorani AA, Gupta KA, Bhadada K and Kale MK: Protective effect of methanolic leaf extract of caesalpinia bonduc on gentamicin-induced hepatotoxicity and nephrotoxicity in rats. IJPT 2011;10: 21-25
[48] Parlakpinar H, Tasdemir S, Polat A, Bay-Karabulut A, Vardi N, Ucar M And Acet A: Protective role of caffeic acid phenethyl ester (cape) on gentamicin-induced acute renal toxicity in rats. Toxicol 2005;207: 169-177
[49] Tavafi M: Protection of renal tubular against gentamicin induced nephrotoxicity. J Ren Inj Prev 2013;2(1):5-6 doi:10.12861/jrip.2013.03
[50] Balakumar P, Rohilla A and Thangathirupathi A: Gentamicin-induced nephrotoxicity: Do we have promising therapeutic approach to blunt it?. Pharmacol Res 2010; 62:179-186
[51] Erdem A, Gundogan NU, Usubutun A, Kilinc K, Erdem SR, Kara A and Bozkurt A: The protective effect of taurine against gentamicin-induced acute tubular necrosis in rats. Nephrol Dial Transplant 2000;15: 1175-1182
[52] Servais H, Van Dersmissen P, Thirion G, Vander Essen G, Van Bambeke F, Tulkens PM and Mingeot-leclercq MP: Gentamicin-induced apoptosis in LLC-PKI cells: involvements of lysosomes and mitochondria. Toxicol Appl Pharmcol 2005;206(3): 321-333
[53] Biswas SK, McClure D, Jimenez LA, Megson IL, Rahman I: Curcumin induces glutathione biosynthesis and inhibit NF-kappa B activation and interleukin-8 release in alveolar epithelial cells. Mechanism of free radical scavenging activity. Anti Red Sign 2005;7: 32-41
[54] Tavafi M and Ahmadvand H: Effect of rosmarinic acid on inhibition of gentamicin induced nephrotoxicity in rats. Tissue and Cell 2011;43(6): 392-397
[55] Moreno S, Scheyer T, Romano CS, Vojnov AA: Antioxidant and antimicrobial activities of rosemary extract linked to their polyphenol composition. Free Radical Research 2006;40(2): 223-231
[56] Abdelkader MA, El-Sammad NM and Taha H: The protective role of rosemary (Rosmarinus Officinalis) in lead acetate induced toxicity in rats. J Appl Sci Res 2012;8(6): 3071-3082
[57] Osman IH and Tantaway AA: Antioxidant activity and protective effects of commercial propolis on gentamicin induced nephrotoxicity in rabbits- in vitro study. Turk J Biochem 2013; 38(4): 409-415
[58] Atta AH, Nasr SM, Mouneir SM, Abdel-Aziem SH and Nassar SA: Egyptian propolis alleviates gentamicin-induced nephrotoxicity in rats. J Advan Chem 2014;6(3): 1109-1119
[59] Park EH and Kahng JH: Suppressive effects of propolis in rats adjuvant arthritis. Arch Pharm Res 1999; 22: 554-8
[60] Moghaddam AH, Javaheiri M, Nabavi SF, Mohdavi MR, Nabavi SM and Ebrahimzadeh MA: Protective role of pleurotus porrigens (Angelʼs wings) against gentamicin-induced nephrotoxicity in mice. Eur Rev For Med Pharmacol Sci 2010;14: 1011-1014
[61] Soliman KM, Abdul-hamid M and Othman AI: Effect of carnosine on gentamicin induced nephrotoxicity. Med Sci Monit 2007;13: 73-83
[62] Sivachandran M and Hariharan P: Renoprotective effect of terminalia chebula on gentamicin induced toxicity in rats. Int J Vet Sci 2012;1(2): 76-79
[63] Salem TA, Bassiouny K, Kabel M, Elsayed IH and El-Kenaway AE: The protective role of propolis on gentamicin induced nephrotoxicity in rats. Sci J Fac Sci 2010; XXIV: 1-13
[64] Sun F, Hayami S, Haruna S, Ogiri Y, Tanaka K and Yamada Y: In vivo antioxidative activity of propolis evaluated by the interaction with vitamin Cand E and the level of lipid hydroperoxides in rats. J Agricult Food Chem 2000;48(5): 1462-1465
Author Information
  • Department of Zoology, Alegelat Faculty of Science, Zawia University, Libya

  • Department of Anatomy, Faculty of Medicine, Zagazig University, Egypt

  • Department of Zoology, Alegelat Faculty of Science, Zawia University, Libya

Cite This Article
  • APA Style

    Azab El Saied Azab, Fathy Ahmed Fetouh, Mohamed Omer Albasha. (2014). Nephro-Protective Effects of Curcumin, Rosemary and Propolis against Gentamicin Induced Toxicity in Guinea Pigs: Morphological and Biochemical Study. American Journal of Clinical and Experimental Medicine, 2(2), 28-35. https://doi.org/10.11648/j.ajcem.20140202.14

    Copy | Download

    ACS Style

    Azab El Saied Azab; Fathy Ahmed Fetouh; Mohamed Omer Albasha. Nephro-Protective Effects of Curcumin, Rosemary and Propolis against Gentamicin Induced Toxicity in Guinea Pigs: Morphological and Biochemical Study. Am. J. Clin. Exp. Med. 2014, 2(2), 28-35. doi: 10.11648/j.ajcem.20140202.14

    Copy | Download

    AMA Style

    Azab El Saied Azab, Fathy Ahmed Fetouh, Mohamed Omer Albasha. Nephro-Protective Effects of Curcumin, Rosemary and Propolis against Gentamicin Induced Toxicity in Guinea Pigs: Morphological and Biochemical Study. Am J Clin Exp Med. 2014;2(2):28-35. doi: 10.11648/j.ajcem.20140202.14

    Copy | Download

  • @article{10.11648/j.ajcem.20140202.14,
      author = {Azab El Saied Azab and Fathy Ahmed Fetouh and Mohamed Omer Albasha},
      title = {Nephro-Protective Effects of Curcumin, Rosemary and Propolis against Gentamicin Induced Toxicity in Guinea Pigs: Morphological and Biochemical Study},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {2},
      number = {2},
      pages = {28-35},
      doi = {10.11648/j.ajcem.20140202.14},
      url = {https://doi.org/10.11648/j.ajcem.20140202.14},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajcem.20140202.14},
      abstract = {The kidney is a common target for toxic xenobiotics due to its capacity  to extract and concentrate toxic substances by highly specialized cells and also, due to its large blood flow. Objective: The present work aimed to evaluate the effectiveness of different natural materials (curcumin, rosemary and propolis) against the histological and also biochemical alterations of gentamicin induced nephrotoxicity in guinea pigs. Materials and methods: 48 guinea pigs were used for this study and divided into 8 groups. The first 4 groups were control groups, the 5th group was the experimental and administered gentamicin at a dose of 100 mg/kg body wt for 10 days, and in the 6th , 7th , and 8th groups, gentamicin was co-administered with curcumin, rosemary, and propolis at the doses of 200 mg, 220 mg, and 100 mg/kg body wt respectively. The animals were sacrificed and the kidneys were dissected and specimens were obtained. The specimens were processed for light microscopic examinations. Blood samples were obtained for assessment of urea, creatinine and uric acid levels. Results: In gentamicin treated animals, there were structural changes. The proximal convoluted tubules showed degenerated epithelial lining with disruption of their brush borders and presence of epithelial debris inside their lumens. The renal corpuscle appeared with degeneration of the glomerulus and disrupted Bowman's capsule. The afferent arteriole showed thickening in its wall and degeneration of endothelial lining with extensive perivascular infiltration of inflammatory cells. Massive interstitial hemorrhage was seen. Also, the serum urea, creatinine, and uric acid were elevated. Co-administration of curcumin, rosemary, and propolis significantly improved the structural changes in the kidney and the blood urea, creatinine and uric acid were significantly declined. Conclusion: It can be concluded that, the gentamicin has adverse effects on the kidney. Different natural materials as curcumin, rosemary, and propolis were able to protect the kidney against these effects. So, the patients should be advised to take one of these materials while they are treated by gentamicin.},
     year = {2014}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Nephro-Protective Effects of Curcumin, Rosemary and Propolis against Gentamicin Induced Toxicity in Guinea Pigs: Morphological and Biochemical Study
    AU  - Azab El Saied Azab
    AU  - Fathy Ahmed Fetouh
    AU  - Mohamed Omer Albasha
    Y1  - 2014/04/20
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajcem.20140202.14
    DO  - 10.11648/j.ajcem.20140202.14
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 28
    EP  - 35
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20140202.14
    AB  - The kidney is a common target for toxic xenobiotics due to its capacity  to extract and concentrate toxic substances by highly specialized cells and also, due to its large blood flow. Objective: The present work aimed to evaluate the effectiveness of different natural materials (curcumin, rosemary and propolis) against the histological and also biochemical alterations of gentamicin induced nephrotoxicity in guinea pigs. Materials and methods: 48 guinea pigs were used for this study and divided into 8 groups. The first 4 groups were control groups, the 5th group was the experimental and administered gentamicin at a dose of 100 mg/kg body wt for 10 days, and in the 6th , 7th , and 8th groups, gentamicin was co-administered with curcumin, rosemary, and propolis at the doses of 200 mg, 220 mg, and 100 mg/kg body wt respectively. The animals were sacrificed and the kidneys were dissected and specimens were obtained. The specimens were processed for light microscopic examinations. Blood samples were obtained for assessment of urea, creatinine and uric acid levels. Results: In gentamicin treated animals, there were structural changes. The proximal convoluted tubules showed degenerated epithelial lining with disruption of their brush borders and presence of epithelial debris inside their lumens. The renal corpuscle appeared with degeneration of the glomerulus and disrupted Bowman's capsule. The afferent arteriole showed thickening in its wall and degeneration of endothelial lining with extensive perivascular infiltration of inflammatory cells. Massive interstitial hemorrhage was seen. Also, the serum urea, creatinine, and uric acid were elevated. Co-administration of curcumin, rosemary, and propolis significantly improved the structural changes in the kidney and the blood urea, creatinine and uric acid were significantly declined. Conclusion: It can be concluded that, the gentamicin has adverse effects on the kidney. Different natural materials as curcumin, rosemary, and propolis were able to protect the kidney against these effects. So, the patients should be advised to take one of these materials while they are treated by gentamicin.
    VL  - 2
    IS  - 2
    ER  - 

    Copy | Download

  • Sections