Nephro-Protective Effects of Curcumin, Rosemary and Propolis against Gentamicin Induced Toxicity in Guinea Pigs: Morphological and Biochemical Study
American Journal of Clinical and Experimental Medicine
Volume 2, Issue 2, March 2014, Pages: 28-35
Received: Mar. 25, 2014; Accepted: Apr. 10, 2014; Published: Apr. 20, 2014
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Azab El Saied Azab, Department of Zoology, Alegelat Faculty of Science, Zawia University, Libya
Fathy Ahmed Fetouh, Department of Anatomy, Faculty of Medicine, Zagazig University, Egypt
Mohamed Omer Albasha, Department of Zoology, Alegelat Faculty of Science, Zawia University, Libya
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The kidney is a common target for toxic xenobiotics due to its capacity to extract and concentrate toxic substances by highly specialized cells and also, due to its large blood flow. Objective: The present work aimed to evaluate the effectiveness of different natural materials (curcumin, rosemary and propolis) against the histological and also biochemical alterations of gentamicin induced nephrotoxicity in guinea pigs. Materials and methods: 48 guinea pigs were used for this study and divided into 8 groups. The first 4 groups were control groups, the 5th group was the experimental and administered gentamicin at a dose of 100 mg/kg body wt for 10 days, and in the 6th , 7th , and 8th groups, gentamicin was co-administered with curcumin, rosemary, and propolis at the doses of 200 mg, 220 mg, and 100 mg/kg body wt respectively. The animals were sacrificed and the kidneys were dissected and specimens were obtained. The specimens were processed for light microscopic examinations. Blood samples were obtained for assessment of urea, creatinine and uric acid levels. Results: In gentamicin treated animals, there were structural changes. The proximal convoluted tubules showed degenerated epithelial lining with disruption of their brush borders and presence of epithelial debris inside their lumens. The renal corpuscle appeared with degeneration of the glomerulus and disrupted Bowman's capsule. The afferent arteriole showed thickening in its wall and degeneration of endothelial lining with extensive perivascular infiltration of inflammatory cells. Massive interstitial hemorrhage was seen. Also, the serum urea, creatinine, and uric acid were elevated. Co-administration of curcumin, rosemary, and propolis significantly improved the structural changes in the kidney and the blood urea, creatinine and uric acid were significantly declined. Conclusion: It can be concluded that, the gentamicin has adverse effects on the kidney. Different natural materials as curcumin, rosemary, and propolis were able to protect the kidney against these effects. So, the patients should be advised to take one of these materials while they are treated by gentamicin.
Nephrotoxicity, Histology, Biochemical, Gentamicin, Curcumin, Rosemary, Propolis
To cite this article
Azab El Saied Azab, Fathy Ahmed Fetouh, Mohamed Omer Albasha, Nephro-Protective Effects of Curcumin, Rosemary and Propolis against Gentamicin Induced Toxicity in Guinea Pigs: Morphological and Biochemical Study, American Journal of Clinical and Experimental Medicine. Vol. 2, No. 2, 2014, pp. 28-35. doi: 10.11648/j.ajcem.20140202.14
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