Detection of Infectious Bovine Rhinotracheitis in Natural Cases of Bovine Abortion by PCR and Histopathology Assays

Farhang Sasani; Aida Vazirian; Javad Javanbakht; Mehdi Aghamohammd Hassan

doi:doi:10.11648/j.ajcem.20130102.11

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Detection of Infectious Bovine Rhinotracheitis in Natural Cases of Bovine Abortion by PCR and Histopathology Assays

Farhang Sasani, Aida Vazirian, Javad Javanbakht, Mehdi Aghamohammd Hassan

Published in American Journal of Clinical and Experimental Medicine (Volume 1, Issue 2)

Received: 29 July 2013 Accepted: Published: 30 August 2013

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Abstract

Bovine herpesvirus 1 (BHV-1), the causative agent of infectious bovine rhinotracheitis (IBR), is considered to be the most common viral pathogen found in bovine. Bovine Herpesvirus 1(BHV-1) belongs to the genus of Varicellovirus and the family of Herpesviridae which contains three main gB, gC and gD genes. The objective of this study was to determine the relation between type of macroscopic lesions and bovine herpesvirus-1(BHV-1) infection; hence the aborted fetus specimens were initially prepared for PCR test. In this study, 23 of fetus samples, which were transferred to Veterinary Laboratories during 2009-2012, were assessed for BHV-1 with PCR in lung and spleen tissues and all of positive samples undergone histopathological study. The results indicated myocarditis and necrosis of cardiac tissue in 8 cases (42.1%), and 6 cases encompassed interstitial lymphocytic pneumonia (28.57%), whereas 5 cases revealed bronchopneumonia (23.8%) and in 8 cases (38.09%) severe interstitial edema was observed. In addition, the lesions may be associated with BHV-1, so that in brain tissue 9 cases (36 %) possessed encephalitis and necrosis, 8 cases (38.09%) indicated interstitial nephritis, 2 cases (9.52%) developed acute tubular necrosis, 2 cases (9.52%) revealed vacuolar degeneration inrenal tubular epithelium, 1 case had (4.76%) renal hemorrhage and in 3 cases (14.28%) renal autolysis was observable. In liver, 10 cases (47.61%) were affected to lymphocytic hepatitis, 5 cases (23.8%) possessed necrotic hepatitis, 6 cases (28.57%) of multifocal coagulative necrosis without inflammatory reaction and 3 cases (14.28%) with lymphocytic portal hepatitis were observed. In this assessment, 10 specimens (52.17%) of spleen tissues revealed necrosis and lymphatic tissues emptying and in 10 cases (100%) severe enteritis, which may be associated with IBR, were recorded. This study suggests based on lesions in several organs induced by IBR virus and real time PCR confirmation there is direct correlation between virus and lesions, and in order to obtain more reliable results of IBR infection the histopathological examination may be efficient.

Published in	American Journal of Clinical and Experimental Medicine (Volume 1, Issue 2)
DOI	10.11648/j.ajcem.20130102.11
Page(s)	35-39
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bovine Herpesvirus-1, Histopathological, Real Time PCR, Lung, Aborted Foetuses

References

[1]	Gibbs EPJ, ReveyemamuMM.Bovine herpesviruses. part 1. Bovine herpesvirus-1. Vet Bull1977;47:317-343.
[2]	Kendrick JW, Schneider L, Straub OC. Placental reac -tion to the infectious bovine rhinotracheitis-infectious pustularvulvovaginitis virus. Am J Vet Res1971;32:1045–1051.
[3]	Molello JA, Chow TL, Owen N, Jensen R. Placental pathology. V. Placental lesions of cattle experimentally infected with infectious bovine rhinotracheitis virus. Am J Vet Res 1966; 27: 907–915.
[4]	Owen NV, Chow TL, MolelloJA. Bovine fetal lesions experimentally produced by infectious bovine rhinotracheitis virus. Am J Vet Res 1964;25:1617–1626
[5]	Rodger SM, Murray J, Underwood C, Buxton D.Microscopical lesions and antigen distribution in bovine fetal tissues and placentae following experimental infection with bovine herpesvirus-1 during pregnancy. J Comp Pathol2007;137: 94–101.
[6]	Smith KC.Herpesviral abortion in domestic animals. Vet J 1997;153:253–268.
[7]	Smith KC, Whitwell KE, Blunden AS. Equine herpesvirus-1 abortion: atypical cases with lesions largely or wholly restricted to the placenta. Equine Vet J 2004;36:79–82.
[8]	Mahajan V, Banga HS, Deka D, Filia G, Gupta A.Comparison of DiagnosticTests for Diagnosis of InfectiousBovineRhinotracheitis in NaturalCases of BovineAbortion. J Comp Pathol.2013 Jul 22.pii: S0021-9975(13)00083-2. doi: 10.1016/j.jcpa.2013.05.002. [Epub ahead of print]
[9]	Wang J, O’Keefe J, Orr D. An international inter-laboratory ring trial to evaluate a real-time PCR assay for the detection of bovine herpesvirus 1 in extended bovine semen. Vet Microbiol2008;126:11–19.
[10]	Sambrook J, Russell DW. Mole-cular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York,2001, pp. 544–553.
[11]	Abril C, Eengles M, Liman A, Hileb M, Albini S. Both viral and host factors contribute to neurovirulence of bovine herpesviruses 1 and 5 in interferon receptor-deficient mice. Virology Journal 2004;78, 3644–3653.
[12]	Lovato L, Inman M, Henderson G, Doster A, Jones C. Infection of cattle with a bovine herpesvirus 1 strain that contains a mutation in the latency related gene leads to increased apoptosis in trigeminal ganglia during the transition from acute infection to latency. Virology Journal 2003;77, 4848–4857.
[13]	Jubb k ,palmer’s.Pathology of domestic animals,fifth edition,2007,volume 1,pp:429-430
[14]	Yamini B, Mullaney TP, Patterson JS, Fitzgerald SD, SteficekBA.Causes of bovine abortion in the north-central United States:Survey of 1618 cases(1983-2001),The bovine practioner2002;38:59-64
[15]	Anderson ML.Infectious causes of bovine abortion during mid- to late-gestation.Theriogenology2007;68(3):474-86.
[16]	Safarpoordehkordi F, Haghighi N, Momtazm H,rafsanjaniS, Momeni M.Conventional vs real-time pcr for detection of bovine herpes virus type 1 in aborted bovine, buffalo and camel fetuses. Bulgarian Journal of Veterinary Medicine 2013;16( 2):102 – 111

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Farhang Sasani, Aida Vazirian, Javad Javanbakht, Mehdi Aghamohammd Hassan. (2013). Detection of Infectious Bovine Rhinotracheitis in Natural Cases of Bovine Abortion by PCR and Histopathology Assays. American Journal of Clinical and Experimental Medicine, 1(2), 35-39. https://doi.org/10.11648/j.ajcem.20130102.11

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ACS Style

Farhang Sasani; Aida Vazirian; Javad Javanbakht; Mehdi Aghamohammd Hassan. Detection of Infectious Bovine Rhinotracheitis in Natural Cases of Bovine Abortion by PCR and Histopathology Assays. Am. J. Clin. Exp. Med. 2013, 1(2), 35-39. doi: 10.11648/j.ajcem.20130102.11

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AMA Style

Farhang Sasani, Aida Vazirian, Javad Javanbakht, Mehdi Aghamohammd Hassan. Detection of Infectious Bovine Rhinotracheitis in Natural Cases of Bovine Abortion by PCR and Histopathology Assays. Am J Clin Exp Med. 2013;1(2):35-39. doi: 10.11648/j.ajcem.20130102.11

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@article{10.11648/j.ajcem.20130102.11,
  author = {Farhang Sasani and Aida Vazirian and Javad Javanbakht and Mehdi Aghamohammd Hassan},
  title = {Detection of Infectious Bovine Rhinotracheitis in Natural Cases of Bovine Abortion by PCR and Histopathology Assays},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {1},
  number = {2},
  pages = {35-39},
  doi = {10.11648/j.ajcem.20130102.11},
  url = {https://doi.org/10.11648/j.ajcem.20130102.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20130102.11},
  abstract = {Bovine  herpesvirus  1 (BHV-1),  the  causative  agent  of  infectious  bovine rhinotracheitis  (IBR),  is  considered  to  be  the  most  common  viral  pathogen  found  in  bovine. Bovine Herpesvirus 1(BHV-1) belongs to the genus of Varicellovirus and the family of Herpesviridae which contains three main gB, gC and gD genes. The objective of this study was to determine the relation between type of macroscopic lesions and bovine herpesvirus-1(BHV-1) infection; hence the aborted fetus specimens were initially prepared for PCR test. In this study, 23 of fetus samples, which were transferred to Veterinary Laboratories during 2009-2012, were assessed for BHV-1 with PCR in lung and spleen tissues and all of positive samples undergone histopathological study. The results indicated myocarditis and necrosis of cardiac tissue in 8 cases (42.1%), and 6 cases encompassed interstitial lymphocytic pneumonia (28.57%), whereas 5 cases revealed bronchopneumonia (23.8%) and in 8 cases (38.09%) severe interstitial edema was observed. In addition, the lesions may be associated with BHV-1, so that in brain tissue 9 cases (36 %) possessed encephalitis and necrosis, 8 cases (38.09%) indicated interstitial nephritis, 2 cases (9.52%) developed acute tubular necrosis, 2 cases (9.52%) revealed vacuolar degeneration inrenal tubular epithelium, 1 case had (4.76%) renal hemorrhage and in 3 cases (14.28%) renal autolysis was observable. In liver, 10 cases (47.61%) were affected to lymphocytic hepatitis, 5 cases (23.8%) possessed necrotic hepatitis, 6 cases (28.57%) of multifocal coagulative necrosis without inflammatory reaction and 3 cases (14.28%) with lymphocytic portal hepatitis were observed. In this assessment, 10 specimens (52.17%) of spleen tissues revealed necrosis and lymphatic tissues emptying and in 10 cases (100%) severe enteritis, which may be associated with IBR, were recorded. This study suggests based on lesions in several organs induced by IBR virus and real time PCR confirmation there is direct correlation between virus and lesions, and in order to obtain more reliable results of IBR infection the histopathological examination may be efficient.},
 year = {2013}
}

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TY  - JOUR
T1  - Detection of Infectious Bovine Rhinotracheitis in Natural Cases of Bovine Abortion by PCR and Histopathology Assays
AU  - Farhang Sasani
AU  - Aida Vazirian
AU  - Javad Javanbakht
AU  - Mehdi Aghamohammd Hassan
Y1  - 2013/08/30
PY  - 2013
N1  - https://doi.org/10.11648/j.ajcem.20130102.11
DO  - 10.11648/j.ajcem.20130102.11
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 35
EP  - 39
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20130102.11
AB  - Bovine  herpesvirus  1 (BHV-1),  the  causative  agent  of  infectious  bovine rhinotracheitis  (IBR),  is  considered  to  be  the  most  common  viral  pathogen  found  in  bovine. Bovine Herpesvirus 1(BHV-1) belongs to the genus of Varicellovirus and the family of Herpesviridae which contains three main gB, gC and gD genes. The objective of this study was to determine the relation between type of macroscopic lesions and bovine herpesvirus-1(BHV-1) infection; hence the aborted fetus specimens were initially prepared for PCR test. In this study, 23 of fetus samples, which were transferred to Veterinary Laboratories during 2009-2012, were assessed for BHV-1 with PCR in lung and spleen tissues and all of positive samples undergone histopathological study. The results indicated myocarditis and necrosis of cardiac tissue in 8 cases (42.1%), and 6 cases encompassed interstitial lymphocytic pneumonia (28.57%), whereas 5 cases revealed bronchopneumonia (23.8%) and in 8 cases (38.09%) severe interstitial edema was observed. In addition, the lesions may be associated with BHV-1, so that in brain tissue 9 cases (36 %) possessed encephalitis and necrosis, 8 cases (38.09%) indicated interstitial nephritis, 2 cases (9.52%) developed acute tubular necrosis, 2 cases (9.52%) revealed vacuolar degeneration inrenal tubular epithelium, 1 case had (4.76%) renal hemorrhage and in 3 cases (14.28%) renal autolysis was observable. In liver, 10 cases (47.61%) were affected to lymphocytic hepatitis, 5 cases (23.8%) possessed necrotic hepatitis, 6 cases (28.57%) of multifocal coagulative necrosis without inflammatory reaction and 3 cases (14.28%) with lymphocytic portal hepatitis were observed. In this assessment, 10 specimens (52.17%) of spleen tissues revealed necrosis and lymphatic tissues emptying and in 10 cases (100%) severe enteritis, which may be associated with IBR, were recorded. This study suggests based on lesions in several organs induced by IBR virus and real time PCR confirmation there is direct correlation between virus and lesions, and in order to obtain more reliable results of IBR infection the histopathological examination may be efficient.
VL  - 1
IS  - 2
ER  -

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Author Information

Farhang Sasani

Department of Pathology, Faculty of Veterinary Medicine, University of Tehran,Tehran, Iran
Aida Vazirian

Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Javad Javanbakht

Department of Pathology, Faculty of Veterinary Medicine, University of Tehran,Tehran, Iran
Mehdi Aghamohammd Hassan

Department of Clinical Science, Faculty of Veterinary Medicine, University of Tehran,Tehran, Iran

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APA Style

Farhang Sasani, Aida Vazirian, Javad Javanbakht, Mehdi Aghamohammd Hassan. (2013). Detection of Infectious Bovine Rhinotracheitis in Natural Cases of Bovine Abortion by PCR and Histopathology Assays. American Journal of Clinical and Experimental Medicine, 1(2), 35-39. https://doi.org/10.11648/j.ajcem.20130102.11

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ACS Style

Farhang Sasani; Aida Vazirian; Javad Javanbakht; Mehdi Aghamohammd Hassan. Detection of Infectious Bovine Rhinotracheitis in Natural Cases of Bovine Abortion by PCR and Histopathology Assays. Am. J. Clin. Exp. Med. 2013, 1(2), 35-39. doi: 10.11648/j.ajcem.20130102.11

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AMA Style

Farhang Sasani, Aida Vazirian, Javad Javanbakht, Mehdi Aghamohammd Hassan. Detection of Infectious Bovine Rhinotracheitis in Natural Cases of Bovine Abortion by PCR and Histopathology Assays. Am J Clin Exp Med. 2013;1(2):35-39. doi: 10.11648/j.ajcem.20130102.11

Copy | Download

@article{10.11648/j.ajcem.20130102.11,
  author = {Farhang Sasani and Aida Vazirian and Javad Javanbakht and Mehdi Aghamohammd Hassan},
  title = {Detection of Infectious Bovine Rhinotracheitis in Natural Cases of Bovine Abortion by PCR and Histopathology Assays},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {1},
  number = {2},
  pages = {35-39},
  doi = {10.11648/j.ajcem.20130102.11},
  url = {https://doi.org/10.11648/j.ajcem.20130102.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20130102.11},
  abstract = {Bovine  herpesvirus  1 (BHV-1),  the  causative  agent  of  infectious  bovine rhinotracheitis  (IBR),  is  considered  to  be  the  most  common  viral  pathogen  found  in  bovine. Bovine Herpesvirus 1(BHV-1) belongs to the genus of Varicellovirus and the family of Herpesviridae which contains three main gB, gC and gD genes. The objective of this study was to determine the relation between type of macroscopic lesions and bovine herpesvirus-1(BHV-1) infection; hence the aborted fetus specimens were initially prepared for PCR test. In this study, 23 of fetus samples, which were transferred to Veterinary Laboratories during 2009-2012, were assessed for BHV-1 with PCR in lung and spleen tissues and all of positive samples undergone histopathological study. The results indicated myocarditis and necrosis of cardiac tissue in 8 cases (42.1%), and 6 cases encompassed interstitial lymphocytic pneumonia (28.57%), whereas 5 cases revealed bronchopneumonia (23.8%) and in 8 cases (38.09%) severe interstitial edema was observed. In addition, the lesions may be associated with BHV-1, so that in brain tissue 9 cases (36 %) possessed encephalitis and necrosis, 8 cases (38.09%) indicated interstitial nephritis, 2 cases (9.52%) developed acute tubular necrosis, 2 cases (9.52%) revealed vacuolar degeneration inrenal tubular epithelium, 1 case had (4.76%) renal hemorrhage and in 3 cases (14.28%) renal autolysis was observable. In liver, 10 cases (47.61%) were affected to lymphocytic hepatitis, 5 cases (23.8%) possessed necrotic hepatitis, 6 cases (28.57%) of multifocal coagulative necrosis without inflammatory reaction and 3 cases (14.28%) with lymphocytic portal hepatitis were observed. In this assessment, 10 specimens (52.17%) of spleen tissues revealed necrosis and lymphatic tissues emptying and in 10 cases (100%) severe enteritis, which may be associated with IBR, were recorded. This study suggests based on lesions in several organs induced by IBR virus and real time PCR confirmation there is direct correlation between virus and lesions, and in order to obtain more reliable results of IBR infection the histopathological examination may be efficient.},
 year = {2013}
}

Copy | Download

TY  - JOUR
T1  - Detection of Infectious Bovine Rhinotracheitis in Natural Cases of Bovine Abortion by PCR and Histopathology Assays
AU  - Farhang Sasani
AU  - Aida Vazirian
AU  - Javad Javanbakht
AU  - Mehdi Aghamohammd Hassan
Y1  - 2013/08/30
PY  - 2013
N1  - https://doi.org/10.11648/j.ajcem.20130102.11
DO  - 10.11648/j.ajcem.20130102.11
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 35
EP  - 39
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20130102.11
AB  - Bovine  herpesvirus  1 (BHV-1),  the  causative  agent  of  infectious  bovine rhinotracheitis  (IBR),  is  considered  to  be  the  most  common  viral  pathogen  found  in  bovine. Bovine Herpesvirus 1(BHV-1) belongs to the genus of Varicellovirus and the family of Herpesviridae which contains three main gB, gC and gD genes. The objective of this study was to determine the relation between type of macroscopic lesions and bovine herpesvirus-1(BHV-1) infection; hence the aborted fetus specimens were initially prepared for PCR test. In this study, 23 of fetus samples, which were transferred to Veterinary Laboratories during 2009-2012, were assessed for BHV-1 with PCR in lung and spleen tissues and all of positive samples undergone histopathological study. The results indicated myocarditis and necrosis of cardiac tissue in 8 cases (42.1%), and 6 cases encompassed interstitial lymphocytic pneumonia (28.57%), whereas 5 cases revealed bronchopneumonia (23.8%) and in 8 cases (38.09%) severe interstitial edema was observed. In addition, the lesions may be associated with BHV-1, so that in brain tissue 9 cases (36 %) possessed encephalitis and necrosis, 8 cases (38.09%) indicated interstitial nephritis, 2 cases (9.52%) developed acute tubular necrosis, 2 cases (9.52%) revealed vacuolar degeneration inrenal tubular epithelium, 1 case had (4.76%) renal hemorrhage and in 3 cases (14.28%) renal autolysis was observable. In liver, 10 cases (47.61%) were affected to lymphocytic hepatitis, 5 cases (23.8%) possessed necrotic hepatitis, 6 cases (28.57%) of multifocal coagulative necrosis without inflammatory reaction and 3 cases (14.28%) with lymphocytic portal hepatitis were observed. In this assessment, 10 specimens (52.17%) of spleen tissues revealed necrosis and lymphatic tissues emptying and in 10 cases (100%) severe enteritis, which may be associated with IBR, were recorded. This study suggests based on lesions in several organs induced by IBR virus and real time PCR confirmation there is direct correlation between virus and lesions, and in order to obtain more reliable results of IBR infection the histopathological examination may be efficient.
VL  - 1
IS  - 2
ER  -

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