Comparison of Light-Emitting Diode Fluorescent Microscopy Against Bright-Field Microscopy and  JD-TB-Antigen Test for the Diagnosis of Pulmonary Tuberculosis

Boja Dufera Taddese; Abraham Tesfaye Bika; Abay Sisay Misganaw; Silvia Blanco Palencia; Chala Chaburte Galata; Abiy Aklilu Teferra

doi:doi:10.11648/j.ajim.20170505.18

Comparison of Light-Emitting Diode Fluorescent Microscopy Against Bright-Field Microscopy and JD-TB-Antigen Test for the Diagnosis of Pulmonary Tuberculosis

Boja Dufera Taddese, Abraham Tesfaye Bika, Abay Sisay Misganaw, Silvia Blanco Palencia, Chala Chaburte Galata, Abiy Aklilu Teferra

Published in American Journal of Internal Medicine (Volume 5, Issue 5)

Received: 30 November 2016 Accepted: 12 December 2016 Published: 26 October 2017

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Abstract

Bright field microscopy using Ziehl-Neelsen (ZN) stained smear has been the major diagnostic technique for the diagnosis of pulmonary tuberculosis and treatment initiation in resource limited settings. However, it has its own limitation in terms of its sensitivity. Various reports have shown superior sensitivity of light emitting-diode fluorescent microscopy (LED-FM) and comparable specificity to bright field microscopy. The aim of the study was to compare the results of Auramine O-stained sputum smears by LED-FM against Ziehl-Neelsen stained sputum smears and JD-TB-Antigen test using TB culture as a reference test. A cross-sectional study was conducted in Southern Nations and Nationality People Regions (SNNPR) of Ethiopia from September 2013 to November 2014. A total of 248 sputum samples were collected from different Health Centers and Hospitals of the study area, analyzed by LED-FM and ZN stained bright field microscopy. The sensitivity, specificity, positive and negative predictive value were 66.12%, 95.28%, 93.02% and 74.69% for ZN stained microscopy; 81.82%, 93.70%, 92.50% and 98.35% for LED–FM, and 58.33%, 63.27%, 53.85% and 67.39% for JD-TB-Antigen test, respectively. The correlation between LED-FM, ZN and culture have shown statistically significant (p<0.001). The sensitivity of LED-FM is better than ZN-stained bright field microscopy. It is also easy to perform, save time and better choice for sputum microscopic examination. The performance of JD-TB-Antigen test was very low for the diagnosis of tuberculosis in sputum specimens. Therefore, further study should be done to use JD-TB-Ag test for diagnosis of pulmonary tuberculosis

Published in	American Journal of Internal Medicine (Volume 5, Issue 5)
DOI	10.11648/j.ajim.20170505.18
Page(s)	105-111
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Ziehl-Neelsen, Auramine-O Stain, LED-FM, TB Culture

References

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[3]	FMoH. National HIV/AIDS Prevention Control Office Technical Document for the AIDS in Ethiopia 6th Report. 2009.
[4]	WHO. Global tuberculosis report. Geneva, Switzerland. 2013.
[5]	Mathur, M. L., Gaur J., Sharma R. and Solanki, A. Rapid culture of Mycobacterium tuberculosis on blood agar in resource limited setting. Dan Med Bull. 2009: 56: 208-210.
[6]	Marais, B. J., Brittle, W. and Painczyk, K. Use of light-emitting diode fluorescence microscopy to detect acid-fast bacilli in sputum. Clin Infect Dis. 2008: 47: 203–207.
[7]	Steingart, K. R., and Henry, M. N. V. Fluorescence versus conventional sputum smear-microscopy for tuberculosis: a systematic review. Lancet Infect Dis. 2006: 6: 570–581.
[8]	Van Deun, A., Chonde, T. M., Gumusboga, M., Rienthong, S. Performance and acceptability of the Fluo LED Easy module for tuberculosis fluorescence microscopy. Int J Tuberc Lung Dis. 2008: 12: 1009–1014.6.
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[10]	Albert, H., Manabe, Y., Lukyamuzi, G., Ademun, H,. Mukkada, S. Nyesiga, B., Joloba, M., Paramasivan, C. N. and Perkins, M. D. Performance of Three LED-Based Fluorescence Microscopy Systems for Detection of Tuberculosis in Uganda. PLoS ONE 2010: 5: 1-7.
[11]	Bonnet, M., Gagnidze, L., Githui, W., Guerin,. P. J., Bonte, L., Varaine.F.e tal. A. Performance of LED-Based Fluorescence Microscopy to Diagnose Tuberculosis in a Peripheral Health Centre in Nairobi. PLoS ONE. 2011: 6: 1-6.
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[14]	Rahman, F., Munshi, S. K., Mostofa Kamal S. M., MatiurRahman, S. M., Rahman, M. M. and Noor, R. Comparison of Different Microscopic Methods with Conventional TB Culture. S. J. Microbiol. 2011: 1: 2074-5356.
[15]	Cattamachi, A., Davis, J. L., Worodria, W., Boon, S., Yoo, S., Matovu, J., e tal. Sensitivity and Specificity of Fluorescence Microscopy for Diagnosing Pulmonary Tuberculosis in a High HIV Prevalence Setting. Int J Tuberc Lung Dis. 2009: 13: 1130–1136.
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[17]	Alifano, M., Pascalis, R. D., Sofia, M., Faraone, S., Pezzo, M., and Covelli, I. Detection of IgG and IgA against the mycobacterial antigen A-60 in patients with extrapulmonary tuberculosis. Thorax. 1998: 53: 377–380.
[18]	Nanta, S., Kantipong, P., Pathipvanich, P., Ruengorn, C., Tawichasri, C.andPatumanond, J. Diagnostic value of an immunochromatographic test over clinical predictors for tuberculosis in HIV patients. Clinical Epidemiology. 2011: 3: 237–244.
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APA Style

Boja Dufera Taddese, Abraham Tesfaye Bika, Abay Sisay Misganaw, Silvia Blanco Palencia, Chala Chaburte Galata, et al. (2017). Comparison of Light-Emitting Diode Fluorescent Microscopy Against Bright-Field Microscopy and JD-TB-Antigen Test for the Diagnosis of Pulmonary Tuberculosis. American Journal of Internal Medicine, 5(5), 105-111. https://doi.org/10.11648/j.ajim.20170505.18

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ACS Style

Boja Dufera Taddese; Abraham Tesfaye Bika; Abay Sisay Misganaw; Silvia Blanco Palencia; Chala Chaburte Galata, et al. Comparison of Light-Emitting Diode Fluorescent Microscopy Against Bright-Field Microscopy and JD-TB-Antigen Test for the Diagnosis of Pulmonary Tuberculosis. Am. J. Intern. Med. 2017, 5(5), 105-111. doi: 10.11648/j.ajim.20170505.18

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AMA Style

Boja Dufera Taddese, Abraham Tesfaye Bika, Abay Sisay Misganaw, Silvia Blanco Palencia, Chala Chaburte Galata, et al. Comparison of Light-Emitting Diode Fluorescent Microscopy Against Bright-Field Microscopy and JD-TB-Antigen Test for the Diagnosis of Pulmonary Tuberculosis. Am J Intern Med. 2017;5(5):105-111. doi: 10.11648/j.ajim.20170505.18

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@article{10.11648/j.ajim.20170505.18,
  author = {Boja Dufera Taddese and Abraham Tesfaye Bika and Abay Sisay Misganaw and Silvia Blanco Palencia and Chala Chaburte Galata and Abiy Aklilu Teferra},
  title = {Comparison of Light-Emitting Diode Fluorescent Microscopy Against Bright-Field Microscopy and  JD-TB-Antigen Test for the Diagnosis of Pulmonary Tuberculosis},
  journal = {American Journal of Internal Medicine},
  volume = {5},
  number = {5},
  pages = {105-111},
  doi = {10.11648/j.ajim.20170505.18},
  url = {https://doi.org/10.11648/j.ajim.20170505.18},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20170505.18},
  abstract = {Bright field microscopy using Ziehl-Neelsen (ZN) stained smear has been the major diagnostic technique for the diagnosis of pulmonary tuberculosis and treatment initiation in resource limited settings. However, it has its own limitation in terms of its sensitivity. Various reports have shown superior sensitivity of light emitting-diode fluorescent microscopy (LED-FM) and comparable specificity to bright field microscopy. The aim of the study was to compare the results of Auramine O-stained sputum smears by LED-FM against Ziehl-Neelsen stained sputum smears and JD-TB-Antigen test using TB culture as a reference test. A cross-sectional study was conducted in Southern Nations and Nationality People Regions (SNNPR) of Ethiopia from September 2013 to November 2014. A total of 248 sputum samples were collected from different Health Centers and Hospitals of the study area, analyzed by LED-FM and ZN stained bright field microscopy. The sensitivity, specificity, positive and negative predictive value were 66.12%, 95.28%, 93.02% and 74.69% for ZN stained microscopy; 81.82%, 93.70%, 92.50% and 98.35% for LED–FM, and 58.33%, 63.27%, 53.85% and 67.39% for JD-TB-Antigen test, respectively. The correlation between LED-FM, ZN and culture have shown statistically significant (p<0.001). The sensitivity of LED-FM is better than ZN-stained bright field microscopy. It is also easy to perform, save time and better choice for sputum microscopic examination. The performance of JD-TB-Antigen test was very low for the diagnosis of tuberculosis in sputum specimens. Therefore, further study should be done to use JD-TB-Ag test for diagnosis of pulmonary tuberculosis},
 year = {2017}
}

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TY  - JOUR
T1  - Comparison of Light-Emitting Diode Fluorescent Microscopy Against Bright-Field Microscopy and  JD-TB-Antigen Test for the Diagnosis of Pulmonary Tuberculosis
AU  - Boja Dufera Taddese
AU  - Abraham Tesfaye Bika
AU  - Abay Sisay Misganaw
AU  - Silvia Blanco Palencia
AU  - Chala Chaburte Galata
AU  - Abiy Aklilu Teferra
Y1  - 2017/10/26
PY  - 2017
N1  - https://doi.org/10.11648/j.ajim.20170505.18
DO  - 10.11648/j.ajim.20170505.18
T2  - American Journal of Internal Medicine
JF  - American Journal of Internal Medicine
JO  - American Journal of Internal Medicine
SP  - 105
EP  - 111
PB  - Science Publishing Group
SN  - 2330-4324
UR  - https://doi.org/10.11648/j.ajim.20170505.18
AB  - Bright field microscopy using Ziehl-Neelsen (ZN) stained smear has been the major diagnostic technique for the diagnosis of pulmonary tuberculosis and treatment initiation in resource limited settings. However, it has its own limitation in terms of its sensitivity. Various reports have shown superior sensitivity of light emitting-diode fluorescent microscopy (LED-FM) and comparable specificity to bright field microscopy. The aim of the study was to compare the results of Auramine O-stained sputum smears by LED-FM against Ziehl-Neelsen stained sputum smears and JD-TB-Antigen test using TB culture as a reference test. A cross-sectional study was conducted in Southern Nations and Nationality People Regions (SNNPR) of Ethiopia from September 2013 to November 2014. A total of 248 sputum samples were collected from different Health Centers and Hospitals of the study area, analyzed by LED-FM and ZN stained bright field microscopy. The sensitivity, specificity, positive and negative predictive value were 66.12%, 95.28%, 93.02% and 74.69% for ZN stained microscopy; 81.82%, 93.70%, 92.50% and 98.35% for LED–FM, and 58.33%, 63.27%, 53.85% and 67.39% for JD-TB-Antigen test, respectively. The correlation between LED-FM, ZN and culture have shown statistically significant (p<0.001). The sensitivity of LED-FM is better than ZN-stained bright field microscopy. It is also easy to perform, save time and better choice for sputum microscopic examination. The performance of JD-TB-Antigen test was very low for the diagnosis of tuberculosis in sputum specimens. Therefore, further study should be done to use JD-TB-Ag test for diagnosis of pulmonary tuberculosis
VL  - 5
IS  - 5
ER  -

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Author Information

Boja Dufera Taddese

Addis Ababa Health Research and Laboratory, Addis Ababa, Ethiopia
Abraham Tesfaye Bika

Addis Ababa Health Research and Laboratory, Addis Ababa, Ethiopia
Abay Sisay Misganaw

Columbia University's Mailman School of Public Health, International Center for AIDS Care and Treatment Program(ICAP), Addis Ababa, Ethiopia
Silvia Blanco Palencia

College of Natural Sciences, Addis Ababa University, Addis Ababa, Ethiopia
Chala Chaburte Galata

Faculty of Veterinary Medicine, Addis Ababa University, Bishoftu, Ethiopia
Abiy Aklilu Teferra

Ethiopian Conformity Assessment Enterprise, Addis Ababa, Ethiopia

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Plain Text BibTeX RIS

APA Style

Boja Dufera Taddese, Abraham Tesfaye Bika, Abay Sisay Misganaw, Silvia Blanco Palencia, Chala Chaburte Galata, et al. (2017). Comparison of Light-Emitting Diode Fluorescent Microscopy Against Bright-Field Microscopy and JD-TB-Antigen Test for the Diagnosis of Pulmonary Tuberculosis. American Journal of Internal Medicine, 5(5), 105-111. https://doi.org/10.11648/j.ajim.20170505.18

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ACS Style

Boja Dufera Taddese; Abraham Tesfaye Bika; Abay Sisay Misganaw; Silvia Blanco Palencia; Chala Chaburte Galata, et al. Comparison of Light-Emitting Diode Fluorescent Microscopy Against Bright-Field Microscopy and JD-TB-Antigen Test for the Diagnosis of Pulmonary Tuberculosis. Am. J. Intern. Med. 2017, 5(5), 105-111. doi: 10.11648/j.ajim.20170505.18

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AMA Style

Boja Dufera Taddese, Abraham Tesfaye Bika, Abay Sisay Misganaw, Silvia Blanco Palencia, Chala Chaburte Galata, et al. Comparison of Light-Emitting Diode Fluorescent Microscopy Against Bright-Field Microscopy and JD-TB-Antigen Test for the Diagnosis of Pulmonary Tuberculosis. Am J Intern Med. 2017;5(5):105-111. doi: 10.11648/j.ajim.20170505.18

Copy | Download

@article{10.11648/j.ajim.20170505.18,
  author = {Boja Dufera Taddese and Abraham Tesfaye Bika and Abay Sisay Misganaw and Silvia Blanco Palencia and Chala Chaburte Galata and Abiy Aklilu Teferra},
  title = {Comparison of Light-Emitting Diode Fluorescent Microscopy Against Bright-Field Microscopy and  JD-TB-Antigen Test for the Diagnosis of Pulmonary Tuberculosis},
  journal = {American Journal of Internal Medicine},
  volume = {5},
  number = {5},
  pages = {105-111},
  doi = {10.11648/j.ajim.20170505.18},
  url = {https://doi.org/10.11648/j.ajim.20170505.18},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20170505.18},
  abstract = {Bright field microscopy using Ziehl-Neelsen (ZN) stained smear has been the major diagnostic technique for the diagnosis of pulmonary tuberculosis and treatment initiation in resource limited settings. However, it has its own limitation in terms of its sensitivity. Various reports have shown superior sensitivity of light emitting-diode fluorescent microscopy (LED-FM) and comparable specificity to bright field microscopy. The aim of the study was to compare the results of Auramine O-stained sputum smears by LED-FM against Ziehl-Neelsen stained sputum smears and JD-TB-Antigen test using TB culture as a reference test. A cross-sectional study was conducted in Southern Nations and Nationality People Regions (SNNPR) of Ethiopia from September 2013 to November 2014. A total of 248 sputum samples were collected from different Health Centers and Hospitals of the study area, analyzed by LED-FM and ZN stained bright field microscopy. The sensitivity, specificity, positive and negative predictive value were 66.12%, 95.28%, 93.02% and 74.69% for ZN stained microscopy; 81.82%, 93.70%, 92.50% and 98.35% for LED–FM, and 58.33%, 63.27%, 53.85% and 67.39% for JD-TB-Antigen test, respectively. The correlation between LED-FM, ZN and culture have shown statistically significant (p<0.001). The sensitivity of LED-FM is better than ZN-stained bright field microscopy. It is also easy to perform, save time and better choice for sputum microscopic examination. The performance of JD-TB-Antigen test was very low for the diagnosis of tuberculosis in sputum specimens. Therefore, further study should be done to use JD-TB-Ag test for diagnosis of pulmonary tuberculosis},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Comparison of Light-Emitting Diode Fluorescent Microscopy Against Bright-Field Microscopy and  JD-TB-Antigen Test for the Diagnosis of Pulmonary Tuberculosis
AU  - Boja Dufera Taddese
AU  - Abraham Tesfaye Bika
AU  - Abay Sisay Misganaw
AU  - Silvia Blanco Palencia
AU  - Chala Chaburte Galata
AU  - Abiy Aklilu Teferra
Y1  - 2017/10/26
PY  - 2017
N1  - https://doi.org/10.11648/j.ajim.20170505.18
DO  - 10.11648/j.ajim.20170505.18
T2  - American Journal of Internal Medicine
JF  - American Journal of Internal Medicine
JO  - American Journal of Internal Medicine
SP  - 105
EP  - 111
PB  - Science Publishing Group
SN  - 2330-4324
UR  - https://doi.org/10.11648/j.ajim.20170505.18
AB  - Bright field microscopy using Ziehl-Neelsen (ZN) stained smear has been the major diagnostic technique for the diagnosis of pulmonary tuberculosis and treatment initiation in resource limited settings. However, it has its own limitation in terms of its sensitivity. Various reports have shown superior sensitivity of light emitting-diode fluorescent microscopy (LED-FM) and comparable specificity to bright field microscopy. The aim of the study was to compare the results of Auramine O-stained sputum smears by LED-FM against Ziehl-Neelsen stained sputum smears and JD-TB-Antigen test using TB culture as a reference test. A cross-sectional study was conducted in Southern Nations and Nationality People Regions (SNNPR) of Ethiopia from September 2013 to November 2014. A total of 248 sputum samples were collected from different Health Centers and Hospitals of the study area, analyzed by LED-FM and ZN stained bright field microscopy. The sensitivity, specificity, positive and negative predictive value were 66.12%, 95.28%, 93.02% and 74.69% for ZN stained microscopy; 81.82%, 93.70%, 92.50% and 98.35% for LED–FM, and 58.33%, 63.27%, 53.85% and 67.39% for JD-TB-Antigen test, respectively. The correlation between LED-FM, ZN and culture have shown statistically significant (p<0.001). The sensitivity of LED-FM is better than ZN-stained bright field microscopy. It is also easy to perform, save time and better choice for sputum microscopic examination. The performance of JD-TB-Antigen test was very low for the diagnosis of tuberculosis in sputum specimens. Therefore, further study should be done to use JD-TB-Ag test for diagnosis of pulmonary tuberculosis
VL  - 5
IS  - 5
ER  -

Copy | Download