Vancomycin continues to be an important antimicrobial agent for treating infections caused by Staphylococcus aureus strains that are resistant to oxacillin (MRSA) and other antimicrobial agents. Vancomycin-resistant Staphylococcus aureus (VRSA) isolates were obtained from HIV-positive patients already on HAART treatment but were not admitted in the hospital. Species identification was confirmed by standard biochemical tests and PCR amplification of the 16S rRNA gene. Vancomycin resistance was determined using the Kirby-Bauer diffusion method and confirmed by Brain Heart Infusion (BHI) vancomycin screen agar plate containing 6µg/ml vancomycin. A total of 8 VRSA were identified from the 59 isolates obtained from the patients. Five out of the eight VRSA isolates were resistant to all the antibiotics tested. However, one unusual strain which was resistant to all the antimicrobial agents tested contained no plasmid, Mec A gene and PVL toxin gene. One VRSA isolate contained a large plasmid (~21.2 kb) and four small plasmids of ~5, 2.5, 1.2 and 0.8 kb respectively. The minimum inhibitory concentration (MIC) for vancomycin susceptibility was >15 µg/ml at disk potency of 30µg. The reduced susceptibility of S. aureus strains to vancomycin leaves clinicians with relatively few therapeutic options for treating these infections and therefore emphasizes the importance of prudent use of antibiotics and the use of infection-control precautions to prevent their transmissions.
| DOI | 10.11648/j.sjph.s.2015030501.11 |
| Published in |
Science Journal of Public Health (Volume 3, Issue 5-1, September 2015)
This article belongs to the Special Issue Who Is Afraid of the Microbes |
| Page(s) | 1-7 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Antibiotic Susceptibility, VRSA, MRSA, HIV Positive Patients, Staphylococcus aureus, Plasmid Profile, MecA Gene, Haemolytic Activities
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APA Style
Emeka-Nwabunnia, Ijeoma, Chiegboka, Nneamaka Alice, Udensi, et al. (2015). Vancomycin-Resistant Staphylococcus aureus Isolates from HIV Positive Patients in Imo State, Nigeria. Science Journal of Public Health, 3(5-1), 1-7. https://doi.org/10.11648/j.sjph.s.2015030501.11
ACS Style
Emeka-Nwabunnia; Ijeoma; Chiegboka; Nneamaka Alice; Udensi, et al. Vancomycin-Resistant Staphylococcus aureus Isolates from HIV Positive Patients in Imo State, Nigeria. Sci. J. Public Health 2015, 3(5-1), 1-7. doi: 10.11648/j.sjph.s.2015030501.11
AMA Style
Emeka-Nwabunnia, Ijeoma, Chiegboka, Nneamaka Alice, Udensi, et al. Vancomycin-Resistant Staphylococcus aureus Isolates from HIV Positive Patients in Imo State, Nigeria. Sci J Public Health. 2015;3(5-1):1-7. doi: 10.11648/j.sjph.s.2015030501.11
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Download@article{10.11648/j.sjph.s.2015030501.11,
author = {Emeka-Nwabunnia and Ijeoma and Chiegboka and Nneamaka Alice and Udensi and Ugochi Justina and Nwaokorie and Francisca Obigaeri},
title = {Vancomycin-Resistant Staphylococcus aureus Isolates from HIV Positive Patients in Imo State, Nigeria},
journal = {Science Journal of Public Health},
volume = {3},
number = {5-1},
pages = {1-7},
doi = {10.11648/j.sjph.s.2015030501.11},
url = {https://doi.org/10.11648/j.sjph.s.2015030501.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.s.2015030501.11},
abstract = {Vancomycin continues to be an important antimicrobial agent for treating infections caused by Staphylococcus aureus strains that are resistant to oxacillin (MRSA) and other antimicrobial agents. Vancomycin-resistant Staphylococcus aureus (VRSA) isolates were obtained from HIV-positive patients already on HAART treatment but were not admitted in the hospital. Species identification was confirmed by standard biochemical tests and PCR amplification of the 16S rRNA gene. Vancomycin resistance was determined using the Kirby-Bauer diffusion method and confirmed by Brain Heart Infusion (BHI) vancomycin screen agar plate containing 6µg/ml vancomycin. A total of 8 VRSA were identified from the 59 isolates obtained from the patients. Five out of the eight VRSA isolates were resistant to all the antibiotics tested. However, one unusual strain which was resistant to all the antimicrobial agents tested contained no plasmid, Mec A gene and PVL toxin gene. One VRSA isolate contained a large plasmid (~21.2 kb) and four small plasmids of ~5, 2.5, 1.2 and 0.8 kb respectively. The minimum inhibitory concentration (MIC) for vancomycin susceptibility was >15 µg/ml at disk potency of 30µg. The reduced susceptibility of S. aureus strains to vancomycin leaves clinicians with relatively few therapeutic options for treating these infections and therefore emphasizes the importance of prudent use of antibiotics and the use of infection-control precautions to prevent their transmissions.},
year = {2015}
}
TY - JOUR T1 - Vancomycin-Resistant Staphylococcus aureus Isolates from HIV Positive Patients in Imo State, Nigeria AU - Emeka-Nwabunnia AU - Ijeoma AU - Chiegboka AU - Nneamaka Alice AU - Udensi AU - Ugochi Justina AU - Nwaokorie AU - Francisca Obigaeri Y1 - 2015/09/02 PY - 2015 N1 - https://doi.org/10.11648/j.sjph.s.2015030501.11 DO - 10.11648/j.sjph.s.2015030501.11 T2 - Science Journal of Public Health JF - Science Journal of Public Health JO - Science Journal of Public Health SP - 1 EP - 7 PB - Science Publishing Group SN - 2328-7950 UR - https://doi.org/10.11648/j.sjph.s.2015030501.11 AB - Vancomycin continues to be an important antimicrobial agent for treating infections caused by Staphylococcus aureus strains that are resistant to oxacillin (MRSA) and other antimicrobial agents. Vancomycin-resistant Staphylococcus aureus (VRSA) isolates were obtained from HIV-positive patients already on HAART treatment but were not admitted in the hospital. Species identification was confirmed by standard biochemical tests and PCR amplification of the 16S rRNA gene. Vancomycin resistance was determined using the Kirby-Bauer diffusion method and confirmed by Brain Heart Infusion (BHI) vancomycin screen agar plate containing 6µg/ml vancomycin. A total of 8 VRSA were identified from the 59 isolates obtained from the patients. Five out of the eight VRSA isolates were resistant to all the antibiotics tested. However, one unusual strain which was resistant to all the antimicrobial agents tested contained no plasmid, Mec A gene and PVL toxin gene. One VRSA isolate contained a large plasmid (~21.2 kb) and four small plasmids of ~5, 2.5, 1.2 and 0.8 kb respectively. The minimum inhibitory concentration (MIC) for vancomycin susceptibility was >15 µg/ml at disk potency of 30µg. The reduced susceptibility of S. aureus strains to vancomycin leaves clinicians with relatively few therapeutic options for treating these infections and therefore emphasizes the importance of prudent use of antibiotics and the use of infection-control precautions to prevent their transmissions. VL - 3 IS - 5-1 ER -
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