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Antibiogram of Bacteria Isolated From Pre-School Children with Asymptomatic Bacteriuria
Science Journal of Public Health
Volume 3, Issue 5-1, September 2015, Pages: 34-39
Received: Aug. 29, 2015; Accepted: Sep. 18, 2015; Published: Oct. 27, 2015
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Authors
Jacinta C. Elo-Ilo, Department of Paediatrics, Nnamdi Azikiwe University Awka / Nnamdi Azikiwe UniversityTeaching Hospital Nnewi, Anambra State, Nigeria
Elochukwu Cajetan Ilo, Department of Pharmacology and Therapeutics, Nnamdi Azikiwe University Awka, Anambra State, Nigeria
Sylvia O. Anyadoh-Nwadike, Department of Biotechnology, Federal University of Technology, Owerri, Imo State, Nigeria
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Abstract
Under five mortality which implies the death of a child before his or her fifth birthday is a major problem in developing countries like Nigeria. Aside other factors; microbial infection is grossly implicated in under five mortality. More problems however are caused by antimicrobial resistant microorganisms. Antimicrobial resistance by bacteria has become a global problem leading to much treatment failure. This study was therefore carried out to ascertain the antimicrobial profile of clinical isolates from pre-nursery school children between the ages of 3 and 5 years with a view to proposing the antibiotic of choice against the common isolates. Known isolates from pre-school children in Nnewi were used for the study. These isolates were subjected to standard microbiological and biochemical protocols to confirm their identities. The disc diffusion method was used to ascertain the susceptibility of the test organisms to commonly used broad spectrum antibiotics. Multidiscs containing ten broad spectrum antibiotics were used. The data obtained from this study were analyzed using the statistical package for social science (SPSS) version 9.5. The mean, range and standard deviation of the variables were determined where applicable. Frequency distribution, percentages and cross tabulation to examine relation between variables was done. The difference between frequencies was tested using Yate’s corrected chi square (χ2) test or Fisher’s exact test. Statistical significance was set at p < 0.05. The overall invitro sensitivity of the isolates was 100% to ciprofloxacin, 90.6% to clindamycin, 68.8% to gentamicin and 59.4% to cefuroxime. Fifty percent of the isolate were sensitive to cephalexim. The isolate were 34.4% sensitive to nitrofuraintoin and nalidixic acid, 25% sensitive to cotrimoxaole and 9.4% sensitive to erythromycin. All isolates were resistant to ampicillin.
Keywords
Antibiogram, Bacteria, Bacteriuria, Resistance
To cite this article
Jacinta C. Elo-Ilo, Elochukwu Cajetan Ilo, Sylvia O. Anyadoh-Nwadike, Antibiogram of Bacteria Isolated From Pre-School Children with Asymptomatic Bacteriuria, Science Journal of Public Health. Special Issue: Who Is Afraid of the Microbes. Vol. 3, No. 5-1, 2015, pp. 34-39. doi: 10.11648/j.sjph.s.2015030501.17
References
[1]
M. Madigan and J. Martinko, Brock Biology of Microorganisms [11th Ed.]. Prentice hall. ISBN. 0131443291.
[2]
M.L. Prescott, P.H. John, A.K. Donald, Textbook of Microbiology, 6th edition, 2005. McGraw Hill, New York.
[3]
K. Todar, Online Textbook of Bacteriology. http://www.textbookofbacteriology.net/e.coli.html, 2009,Retrieved 30/06/2015.
[4]
J. Jawetz, J.L. Melnick, E.A. Adelberg, et al., Medical Microbiology. 21st ed. Appleton and lange, connecticut, U.S.A, 1998.
[5]
H.C. Davision, J.C. Low and M.E. J. Woolhouse, What is antibiotic resistance and how can we measure it? Trends Microbiol. 8: 554 -559, 2000.
[6]
X.T. Cao, R. Kneen, T.A. Nguyen and D.L. Truong, “A comparative study of urinary tract infection, African Journal of Health Science, vol. 18 (3), PP 245 – 248, 1999.
[7]
I.N. Okeke, A. Lamikanra and R. Edeman, “Socioeconomic and behavioural factors leading to acquired to acquired bacterial resistance to antibiotics in developing countries. Emerging Infectious Diseases, 1999, vol 5: 18 – 27.
[8]
I.N. Okeke, O.A. Aboderin, D.K. Byarugaba, K.K. Ojo and J.A. Opintan, “Growing problem of multi-drug resistant enteric pathogens in Africa. http://www.cdc.gov/EID/adherence and compliance to drug prescription and content/13/11/1640.htm. 2007Retrieved 10/09/2014.
[9]
H.C. Neu, “The Crisis in Antibiotic Resistance. Science,” 257: 1064 -1072, 1992.
[10]
S.O. Anyadoh, J. Akerele, U. Udum, “Prevalence of multidrug resistant Escherichia coli among pregnant Women in Owerri,” International Journal of Medical Sciences and Technology, India, 2010, vol. 3 (3):17-20
[11]
B. Blomberg, B.E. Olsen, S.G. Hinderaker, N. Langeland, P. Gasheka,“Antimicrobial resistance in urinary bacterial isolates from pregnancy women in rural Tanzania: Implications for public health,” Scand J Infect Dis 2005; 37:262- 268.
[12]
S.L.M. Dairiki, “How serious is asymptomatic bacteriuria in children?” Contemp. Urol. 1998, vol. 10, PP 65 -69.
[13]
L.E. Nicolle, S. Bradley, R. Colgan, J.C. Rice, A. Schaeffer, T.M. Hooton, “Infectious Diseases Society of America guidelines for the diagnosis and treatment of asymptomatic bacteriuria in adults. Clinical Infectious Diseases,” 2005, 40(5):643-54.
[14]
J. Akerele, and F. Okonofua, “Prevalence of asymptomatic genital infection among pregnant women in Benin-city, Nigeria,” African Journal of Reproductive Health, 2002, vol. 6(3): 93-97.
[15]
L.B. Travis and L.B. Brouhard, The genitourinary system. In: A.M. Rudolph, J.I.E. Hoffman and C.D. Rudolph eds. Rudolph Paediatrics. USA: Appleton and Lange. 1996, PP. 1388-92.
[16]
J. C. Elo-Ilo, M.O. Iroezindu, I. Egbuonu, C.C. Ezechukwu and J.O. Chukwuka, “Prevalence of asymptomatic bacteriuria among pre-school children in Nnewi, South East Nigeria,” Niger. J. Paed. Vol. 30, Issue 3; PP. 278 – 283, 8th Jan. 2013.
[17]
M. Cheesbrough, Antimicrobial sensitivity tests In: Cheesbrough M ED. Medical laboratory manual for tropical countries vol. 11: Microbiology. London: Butterwort-Hienemann 1986 :196-205.
[18]
Clinical and Laboratory Standards Institute (CLSI), Performance Standards for Antimicrobial susceptibility testing; 19th informational supplement M100-S19, 2009, CLSI, Wayne.
[19]
M. O. Ibadin, “The prevalence of urinary tract infection in childhood nephritic syndrome,” Nig J Paediatr 1997; 24:40-44.
[20]
A.A. Adeyemo, R.A. Gbadegesin, T.N. Onyemenam, C.C. Ekweozor, “Urinary tract pathogens and microbial sensitivity pattern in childhood in Ibadan,” Ann Trop paeditr. 1994: 14:271-274.
[21]
A.L. Rabasa, D. Shattima, “Urinary tract infection in severely malnourished children at the University of Maiduguri Teaching Hospital,” J Trop Pediatr 2002 ; 48:359-16.
[22]
J. Akerele, F. Abhullimen, J. Okonofua, “Prevalence of asymptomatic bacteriuria among pregnant women in Benin City, Nigeria,” J Obstet Gynaaecol 2001, vol. 21: 141-4.
[23]
B. J. Brown, A.O. Asinobi, O.J. Fatunde, K. Osinusi, N. A. Fasina,“Antimicrobial sensitivity pattern of organisms causing urinary tract infection in children with sickle cell anaemia in Ibadan, Nigeria,” West African Journal of Medicine, 2003.
[24]
M.O. Ibadin, “Childhood urinary tract infections in Benin city, pathogens and antimicrobial sensititvity pattern,” Jnl Med and Biomed Res 2002, vol. 1:22-8.
[25]
Sylvia. O. Anyadoh-Nwadike , Sylvester. I. Okorondu, Ifeanyi .O.C. Obiajuru, Peter O. Nwadike, F.O. Nwaokorie and John .O. Akerele, “Comparative Study of the Prevalence and Antibiogram of Bacterial Isolates from the Urinary and Genital Tracts of Antenatal Patients,” IOSR Journal of Pharmacy and Biological Sciences Volume 10, Issue 1 Ver. III (Jan -Feb. 2015), PP 15-19.
[26]
A.P. Jr. William, Antimicrobial agents In: Hardman JG, Limbird LE, eds Goodman and Gilmans pharmacological basis of therapeutics, USA: McGraw-Hill , 2001, 1179-85.
[27]
R. Doyle, K. Golberg, C.E. Harlods, J. Poeggd eds.,Handbook of Pediatric Drug therapy Pennsylvanian Spring house corporation, 2000, 167-9.
[28]
M.O. Ibadin, P.O. Abiodun, Urinary tract infection in children with Acute Nephritic syndrome. Ann. Biomed Sci. 2000, vol. 1:23-9.
[29]
A. Onanuga, A.R. Oyi, J.A. Onaolapo,“Prevalence and susceptibility pattern of methicillin resistant Staphylococcus aureus isolates among healthy women in Zaria, Nigeria,” African Journal of Biotechnology, 2005, 4: 1321-1324.
[30]
N.R. Gupta, T. Mattoo, “Urinary tract infection in children, synopsis: A current survey of world literature in Paediatrics,” 2002, vol 4.
[31]
T.A. Olarenwaju, “Prevention of urinary tract infection in children in the tropics,” Post Grad Doct Afr. 2002, vol. 24: 29-31.
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