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Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients
Science Journal of Public Health
Volume 8, Issue 3, May 2020, Pages: 77-82
Received: May 6, 2020; Accepted: May 22, 2020; Published: Jun. 4, 2020
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Authors
Liu Guihua, Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Huang Xin, Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Zhang Weiyu, Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Wang Yanqiu, Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
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Abstract
Objective: To detect the drug resistance of Salmonella isolated from the faeces samples of patients with food-borne diseases, and to know the drug resistance status and development trend of food-borne pathogens in our province. Methods: Enrichment, color medium separation, API20E biochemistry and Thai serum Identification. The minimal inhibitory concentration (MIC) of Salmonella to 15 kinds of antibiotics were quantitatively determined by micro broth dilution method. Results: 123 food-borne Salmonella strains were resistant to ampicillin (AMP), NALIDIXIC acid (NAL), tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ampicillin / sulbactam (AMS), CEFAZOLIN (CFZ), chloramphenicol (CHL), CEFOTAXIME (CTX), ciprofloxacin (CIP), azithromycin (AZM), gentamicin (GEN), cefoxitin (CFX), ceftazidime (CAZ). The drug resistance rates were 87.80%, 84.6%, 69.1%, 50.4%, 39.0%, 34.1%, 26.0%, 26.0%, 26.0%, 24.3%, 19.5%, 2.4% and 0.8% respectively. IMP and ERY were both sensitive. Among them, 90 strains were resistant to more than 3 kinds of antibiotics, and the multidrug resistance rate was 73.2%. MIC results ranged from 1μg / mL to > 64 μg / mL. The total strains were divided into 15 serotypings, including Salmonella Enteritidis (58.5%), Salmonella Typhimurium (16.3%), and Salmonella Stanley (4.9%). The most common distribution of the 57 resistant strains were AMP-TET-NAL-and AMP-NAL-11 strains, followed by NAL-10 strains, AMP-AMS-TET-NAL-7 strains, and multi-resistant strains ranged from 3 to 11 antibiotics. Conclusion: The serotypings of Salmonella isolated from faeces samples of patients from food borne disease surveillance sites in hospitals in Jilin province were mainly S. Enteritis and S. Enterica, and the strains were resistant to multiple antibiotics. The surveillance of drug resistance of Salmonella in food-borne diseases should be strengthened to ensure food safety and human health.
Keywords
Foodborne Diseases, Broth Dilution, MIC, Salmonella, Results Analysis
To cite this article
Liu Guihua, Huang Xin, Zhang Weiyu, Wang Yanqiu, Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients, Science Journal of Public Health. Vol. 8, No. 3, 2020, pp. 77-82. doi: 10.11648/j.sjph.20200803.13
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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