Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients
Science Journal of Public Health
Volume 8, Issue 3, May 2020, Pages: 77-82
Received: May 6, 2020;
Accepted: May 22, 2020;
Published: Jun. 4, 2020
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Liu Guihua, Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Huang Xin, Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Zhang Weiyu, Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Wang Yanqiu, Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
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Objective: To detect the drug resistance of Salmonella isolated from the faeces samples of patients with food-borne diseases, and to know the drug resistance status and development trend of food-borne pathogens in our province. Methods: Enrichment, color medium separation, API20E biochemistry and Thai serum Identification. The minimal inhibitory concentration (MIC) of Salmonella to 15 kinds of antibiotics were quantitatively determined by micro broth dilution method. Results: 123 food-borne Salmonella strains were resistant to ampicillin (AMP), NALIDIXIC acid (NAL), tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ampicillin / sulbactam (AMS), CEFAZOLIN (CFZ), chloramphenicol (CHL), CEFOTAXIME (CTX), ciprofloxacin (CIP), azithromycin (AZM), gentamicin (GEN), cefoxitin (CFX), ceftazidime (CAZ). The drug resistance rates were 87.80%, 84.6%, 69.1%, 50.4%, 39.0%, 34.1%, 26.0%, 26.0%, 26.0%, 24.3%, 19.5%, 2.4% and 0.8% respectively. IMP and ERY were both sensitive. Among them, 90 strains were resistant to more than 3 kinds of antibiotics, and the multidrug resistance rate was 73.2%. MIC results ranged from 1μg / mL to > 64 μg / mL. The total strains were divided into 15 serotypings, including Salmonella Enteritidis (58.5%), Salmonella Typhimurium (16.3%), and Salmonella Stanley (4.9%). The most common distribution of the 57 resistant strains were AMP-TET-NAL-and AMP-NAL-11 strains, followed by NAL-10 strains, AMP-AMS-TET-NAL-7 strains, and multi-resistant strains ranged from 3 to 11 antibiotics. Conclusion: The serotypings of Salmonella isolated from faeces samples of patients from food borne disease surveillance sites in hospitals in Jilin province were mainly S. Enteritis and S. Enterica, and the strains were resistant to multiple antibiotics. The surveillance of drug resistance of Salmonella in food-borne diseases should be strengthened to ensure food safety and human health.
Foodborne Diseases, Broth Dilution, MIC, Salmonella, Results Analysis
To cite this article
Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients, Science Journal of Public Health.
Vol. 8, No. 3,
2020, pp. 77-82.
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
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Zhang Ling, Liu Guihua. Quantitative detection and Risk Assessment of Salmonella in chicken carcasses [J]. Chinese Journal of microecology, 2015, v 27 (8): 907-909.
Du Xiongwei, Jiang Jie, Li Yan, etc. Detection and analysis of drug resistance of Salmonella Suis [J]. Journal of Dalian Nationalities University, 2017, v 19 (1): 33-35.
Liu Guihua, Guo Changhong, Zhou Fengyan, etc. Analysis of enterococcus contamination and detection results in animal food chain in Jilin Province [J]. China health engineering, 2019, v 18 (6): 815-818.
Liu Guihua, Huang Xin, Zhang Weiyu, Gong Yunwei. Detection and analysis of drug resistance of Salmonella in stool of patients with food-borne diseases [J]. Chinese journal of public health engingeering 2018, v. 17 (01): 25-28.
Li Yueting, Gong Yunwei, Liu Guihua. Analysis of drug resistance of food-borne Salmonella and laxative ESCHERICHIA coli [J]. Chinese Journal of Health Inspection, 2016, v. 26 (22): 3335-3337.
Ministry of Health of the People's Republic of China. GB 4789.4-2016 National Standard for Food Safety Food Microbiology, Salmonella [S]. Beijing: China Standard Publishing, 2017.
Chaudun, Liu Guihua, Qu Li. Analysis of detection results of Salmonella by MIC drug sensitive kit of broth dilution method [J]. Chinese journal of public health engingeering, 2014, v. 13 (4): 314-319.
Li Shunji, Liu Guihua. Detection and analysis of drug resistance of Listeria monocytogene in foods with monocyte proliferation [J]. Chinese Journal of Health Inspection, 2015, v. 25 (20): 3772 -3774.
Li Yueting, Gong Yunwei, Liu Guihua. Analysis of drug resistance of Staphylococcus aureus in Jilin Province in 2014 [J]. Chinese Journal of Health Inspection, 2015, v. 25 (21): 3586-3587.
Zhao Wei, Liu Guihua, Wang Yanqiu, etc. Investigation on contamination and drug resistance of Liszt in food [J]. Chinese Journal of Health Inspection, 2012, v. 22 (6): 1394-1395.
Li Shunji, Wang Shudong, Cao Tiehong, Yin Dexiu, Chen Shengchen, Liu Guihua. Drug sensitivity test and result analysis of 41 strains of diarrhea-causing ESCHERICHIA coli [J]. Chinese journal of public health engingeering, 2016, v. 15 (2): 127-129.
Ren Huijing, Liu Yun. Current status of research on drug resistance of foodborne pathogens [J]. Henan Agriculture, 2013, No. 324 (16): 57+59.
Yang Yang, Fu Ping, Guo Yunchang, etc. Trend of Listeria monocytogene resistance in food-borne mononucleosis in China in 2005 [J]. Health Research, 2008, v. 37 (2): 183-186.
Cui Huixia, Li Wenjie, Zhang Xiuli, etc. SEROTYPING and drug resistance analysis of food-borne Liszt in Henan Province in 2012 [J]. Chinese Journal of Health Inspection, 2014, v. 24 (12): 1800-1803.
Xu Yunmin, Du Yan, Dan Bin, etc.. Surveillance of drug resistance of Salmonella Spp. in CHINET from 2005 to 2014 [J]. Chinese Journal of Infection and chemotherapy, 2016, v. 16 (3): 294 -301.
Wang Fang. Research progress of multi-drug Resistance Gene Island 1 of Salmonella [J]. Chinese Journal of Antibiotics, 2010, v. 35 (06): 414-420.
Zhang Rui, Zhao Xueqin, Shang Xiaochun, etc.. Composition and drug resistance of pathogenic bacteria of infantile diarrhea in Hangzhou [J]. Chinese Journal of Health Inspection, 2015, v. 25 (06): 901-903+905.