A Comparative Study on Electrochemical Determination of Vitamin C in Liver and Tomato Using Platinum and Glassy Carbon Electrodes (Original Article)

Tsegaye Tadesse; Assefa Sirgawie

doi:doi:10.11648/j.bmb.20170203.11

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A Comparative Study on Electrochemical Determination of Vitamin C in Liver and Tomato Using Platinum and Glassy Carbon Electrodes (Original Article)

Tsegaye Tadesse, Assefa Sirgawie

Published in Biochemistry and Molecular Biology (Volume 2, Issue 3)

Received: 12 December 2016 Accepted: 29 December 2016 Published: 27 May 2017

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Abstract

Ascorbic acid, a water-soluble vitamin, is the most common electroactive biological compound found in most biological species. The electrochemical oxidation of vitamin C was investigated at GCE and Pt electrodes in various aqueous solutions in the pH range of 1 to 5 (0.1 M KCl as a supporting electrolyte) by CV and DPV. Experimental conditions, for CV: Scan rate of 50 mV/s, Initial potential -100 mV and Final Potential 1000 mV, for DPV: Scan Rate 50mV/s, Pulse amplitude 50mV,Pulse period 125 ms, Initial potential -100mV and Final Potential 1000 mV. For cyclic voltammetry, Regression equation of y=23.4611X + 13.2489 for GCE and y=5.19714X + 13.7071 Pt; LOD of 0.0035294 mM for GCE and 0.0176 mM for Pt; LOQ of 0.025519 mM for GCE and 0.085066 mMPt; R.S.D of % 2.76% for GCE and 4.42% for Pt. And for DPV Regression equation, y = 1.201X + 0.530393 for GCE andy = 0.0521393X + 0.506857 for Pt, R.S.D % 0.391% for GCE and 4.969% for Pt, LOD 0.12412 mM for GCE and 0.22497 mM for Pt and LOQ 0.4137 mM for GCEand 0.7499 mM for Pt. The oxidation peak potential of ascorbic acid were 270 mV and 370 mV for GCE in CV and DPV respectively but for Pt electrode 490 mV for CV and 370 mV for DPV (versus Ag/AgCl reference electrode). The influence of the operational parameters like scan rate, pulse amplitude, pulse period, concentration and pH on the analytical signal was investigated. The method developed by standard was applied to ascorbic acid assessment in liver and tomato samples. The results of ascorbic acid assessment by DPV were compared to those obtained by CV on both GCE and Pt electrodes.

Published in	Biochemistry and Molecular Biology (Volume 2, Issue 3)
DOI	10.11648/j.bmb.20170203.11
Page(s)	25-36
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Vitamin C, Cyclic Voltammetry, Differential Pulse Voltammetry, Glassy Carbon Electrode, Platinum Electrode, Liver, Tomato

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Tsegaye Tadesse, Assefa Sirgawie. (2017). A Comparative Study on Electrochemical Determination of Vitamin C in Liver and Tomato Using Platinum and Glassy Carbon Electrodes (Original Article). Biochemistry and Molecular Biology, 2(3), 25-36. https://doi.org/10.11648/j.bmb.20170203.11

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ACS Style

Tsegaye Tadesse; Assefa Sirgawie. A Comparative Study on Electrochemical Determination of Vitamin C in Liver and Tomato Using Platinum and Glassy Carbon Electrodes (Original Article). Biochem. Mol. Biol. 2017, 2(3), 25-36. doi: 10.11648/j.bmb.20170203.11

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AMA Style

Tsegaye Tadesse, Assefa Sirgawie. A Comparative Study on Electrochemical Determination of Vitamin C in Liver and Tomato Using Platinum and Glassy Carbon Electrodes (Original Article). Biochem Mol Biol. 2017;2(3):25-36. doi: 10.11648/j.bmb.20170203.11

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@article{10.11648/j.bmb.20170203.11,
  author = {Tsegaye Tadesse and Assefa Sirgawie},
  title = {A Comparative Study on Electrochemical Determination of Vitamin C in Liver and Tomato Using Platinum and Glassy Carbon Electrodes (Original Article)},
  journal = {Biochemistry and Molecular Biology},
  volume = {2},
  number = {3},
  pages = {25-36},
  doi = {10.11648/j.bmb.20170203.11},
  url = {https://doi.org/10.11648/j.bmb.20170203.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20170203.11},
  abstract = {Ascorbic acid, a water-soluble vitamin, is the most common electroactive biological compound found in most biological species. The electrochemical oxidation of vitamin C was investigated at GCE and Pt electrodes in various aqueous solutions in the pH range of 1 to 5 (0.1 M KCl as a supporting electrolyte) by CV and DPV. Experimental conditions, for CV: Scan rate of 50 mV/s, Initial potential -100 mV and Final Potential 1000 mV, for DPV: Scan Rate 50mV/s, Pulse amplitude 50mV,Pulse period 125 ms, Initial potential -100mV and Final Potential 1000 mV. For cyclic voltammetry, Regression equation of y=23.4611X + 13.2489 for GCE and y=5.19714X + 13.7071 Pt; LOD of 0.0035294 mM for GCE and 0.0176 mM for Pt; LOQ of 0.025519 mM for GCE and 0.085066 mMPt; R.S.D of % 2.76% for GCE and 4.42% for Pt. And for DPV Regression equation, y = 1.201X + 0.530393 for GCE andy = 0.0521393X + 0.506857 for Pt, R.S.D % 0.391% for GCE and 4.969% for Pt, LOD 0.12412 mM for GCE and 0.22497 mM for Pt and LOQ 0.4137 mM for GCEand 0.7499 mM for Pt. The oxidation peak potential of ascorbic acid were 270 mV and 370 mV for GCE in CV and DPV respectively but for Pt electrode 490 mV for CV and 370 mV for DPV (versus Ag/AgCl reference electrode). The influence of the operational parameters like scan rate, pulse amplitude, pulse period, concentration and pH on the analytical signal was investigated. The method developed by standard was applied to ascorbic acid assessment in liver and tomato samples. The results of ascorbic acid assessment by DPV were compared to those obtained by CV on both GCE and Pt electrodes.},
 year = {2017}
}

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TY  - JOUR
T1  - A Comparative Study on Electrochemical Determination of Vitamin C in Liver and Tomato Using Platinum and Glassy Carbon Electrodes (Original Article)
AU  - Tsegaye Tadesse
AU  - Assefa Sirgawie
Y1  - 2017/05/27
PY  - 2017
N1  - https://doi.org/10.11648/j.bmb.20170203.11
DO  - 10.11648/j.bmb.20170203.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 25
EP  - 36
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20170203.11
AB  - Ascorbic acid, a water-soluble vitamin, is the most common electroactive biological compound found in most biological species. The electrochemical oxidation of vitamin C was investigated at GCE and Pt electrodes in various aqueous solutions in the pH range of 1 to 5 (0.1 M KCl as a supporting electrolyte) by CV and DPV. Experimental conditions, for CV: Scan rate of 50 mV/s, Initial potential -100 mV and Final Potential 1000 mV, for DPV: Scan Rate 50mV/s, Pulse amplitude 50mV,Pulse period 125 ms, Initial potential -100mV and Final Potential 1000 mV. For cyclic voltammetry, Regression equation of y=23.4611X + 13.2489 for GCE and y=5.19714X + 13.7071 Pt; LOD of 0.0035294 mM for GCE and 0.0176 mM for Pt; LOQ of 0.025519 mM for GCE and 0.085066 mMPt; R.S.D of % 2.76% for GCE and 4.42% for Pt. And for DPV Regression equation, y = 1.201X + 0.530393 for GCE andy = 0.0521393X + 0.506857 for Pt, R.S.D % 0.391% for GCE and 4.969% for Pt, LOD 0.12412 mM for GCE and 0.22497 mM for Pt and LOQ 0.4137 mM for GCEand 0.7499 mM for Pt. The oxidation peak potential of ascorbic acid were 270 mV and 370 mV for GCE in CV and DPV respectively but for Pt electrode 490 mV for CV and 370 mV for DPV (versus Ag/AgCl reference electrode). The influence of the operational parameters like scan rate, pulse amplitude, pulse period, concentration and pH on the analytical signal was investigated. The method developed by standard was applied to ascorbic acid assessment in liver and tomato samples. The results of ascorbic acid assessment by DPV were compared to those obtained by CV on both GCE and Pt electrodes.
VL  - 2
IS  - 3
ER  -

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Tsegaye Tadesse

Department of Chemistry, Mizan Tepi University, Tepi, Ethiopia
Assefa Sirgawie

Department of Chemistry, Addis Ababa Science and Technology University, Addis Ababa, Ethiopia

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Tsegaye Tadesse, Assefa Sirgawie. (2017). A Comparative Study on Electrochemical Determination of Vitamin C in Liver and Tomato Using Platinum and Glassy Carbon Electrodes (Original Article). Biochemistry and Molecular Biology, 2(3), 25-36. https://doi.org/10.11648/j.bmb.20170203.11

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ACS Style

Tsegaye Tadesse; Assefa Sirgawie. A Comparative Study on Electrochemical Determination of Vitamin C in Liver and Tomato Using Platinum and Glassy Carbon Electrodes (Original Article). Biochem. Mol. Biol. 2017, 2(3), 25-36. doi: 10.11648/j.bmb.20170203.11

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AMA Style

Tsegaye Tadesse, Assefa Sirgawie. A Comparative Study on Electrochemical Determination of Vitamin C in Liver and Tomato Using Platinum and Glassy Carbon Electrodes (Original Article). Biochem Mol Biol. 2017;2(3):25-36. doi: 10.11648/j.bmb.20170203.11

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@article{10.11648/j.bmb.20170203.11,
  author = {Tsegaye Tadesse and Assefa Sirgawie},
  title = {A Comparative Study on Electrochemical Determination of Vitamin C in Liver and Tomato Using Platinum and Glassy Carbon Electrodes (Original Article)},
  journal = {Biochemistry and Molecular Biology},
  volume = {2},
  number = {3},
  pages = {25-36},
  doi = {10.11648/j.bmb.20170203.11},
  url = {https://doi.org/10.11648/j.bmb.20170203.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20170203.11},
  abstract = {Ascorbic acid, a water-soluble vitamin, is the most common electroactive biological compound found in most biological species. The electrochemical oxidation of vitamin C was investigated at GCE and Pt electrodes in various aqueous solutions in the pH range of 1 to 5 (0.1 M KCl as a supporting electrolyte) by CV and DPV. Experimental conditions, for CV: Scan rate of 50 mV/s, Initial potential -100 mV and Final Potential 1000 mV, for DPV: Scan Rate 50mV/s, Pulse amplitude 50mV,Pulse period 125 ms, Initial potential -100mV and Final Potential 1000 mV. For cyclic voltammetry, Regression equation of y=23.4611X + 13.2489 for GCE and y=5.19714X + 13.7071 Pt; LOD of 0.0035294 mM for GCE and 0.0176 mM for Pt; LOQ of 0.025519 mM for GCE and 0.085066 mMPt; R.S.D of % 2.76% for GCE and 4.42% for Pt. And for DPV Regression equation, y = 1.201X + 0.530393 for GCE andy = 0.0521393X + 0.506857 for Pt, R.S.D % 0.391% for GCE and 4.969% for Pt, LOD 0.12412 mM for GCE and 0.22497 mM for Pt and LOQ 0.4137 mM for GCEand 0.7499 mM for Pt. The oxidation peak potential of ascorbic acid were 270 mV and 370 mV for GCE in CV and DPV respectively but for Pt electrode 490 mV for CV and 370 mV for DPV (versus Ag/AgCl reference electrode). The influence of the operational parameters like scan rate, pulse amplitude, pulse period, concentration and pH on the analytical signal was investigated. The method developed by standard was applied to ascorbic acid assessment in liver and tomato samples. The results of ascorbic acid assessment by DPV were compared to those obtained by CV on both GCE and Pt electrodes.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - A Comparative Study on Electrochemical Determination of Vitamin C in Liver and Tomato Using Platinum and Glassy Carbon Electrodes (Original Article)
AU  - Tsegaye Tadesse
AU  - Assefa Sirgawie
Y1  - 2017/05/27
PY  - 2017
N1  - https://doi.org/10.11648/j.bmb.20170203.11
DO  - 10.11648/j.bmb.20170203.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 25
EP  - 36
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20170203.11
AB  - Ascorbic acid, a water-soluble vitamin, is the most common electroactive biological compound found in most biological species. The electrochemical oxidation of vitamin C was investigated at GCE and Pt electrodes in various aqueous solutions in the pH range of 1 to 5 (0.1 M KCl as a supporting electrolyte) by CV and DPV. Experimental conditions, for CV: Scan rate of 50 mV/s, Initial potential -100 mV and Final Potential 1000 mV, for DPV: Scan Rate 50mV/s, Pulse amplitude 50mV,Pulse period 125 ms, Initial potential -100mV and Final Potential 1000 mV. For cyclic voltammetry, Regression equation of y=23.4611X + 13.2489 for GCE and y=5.19714X + 13.7071 Pt; LOD of 0.0035294 mM for GCE and 0.0176 mM for Pt; LOQ of 0.025519 mM for GCE and 0.085066 mMPt; R.S.D of % 2.76% for GCE and 4.42% for Pt. And for DPV Regression equation, y = 1.201X + 0.530393 for GCE andy = 0.0521393X + 0.506857 for Pt, R.S.D % 0.391% for GCE and 4.969% for Pt, LOD 0.12412 mM for GCE and 0.22497 mM for Pt and LOQ 0.4137 mM for GCEand 0.7499 mM for Pt. The oxidation peak potential of ascorbic acid were 270 mV and 370 mV for GCE in CV and DPV respectively but for Pt electrode 490 mV for CV and 370 mV for DPV (versus Ag/AgCl reference electrode). The influence of the operational parameters like scan rate, pulse amplitude, pulse period, concentration and pH on the analytical signal was investigated. The method developed by standard was applied to ascorbic acid assessment in liver and tomato samples. The results of ascorbic acid assessment by DPV were compared to those obtained by CV on both GCE and Pt electrodes.
VL  - 2
IS  - 3
ER  -

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