The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis
Biochemistry and Molecular Biology
Volume 3, Issue 3, May 2018, Pages: 49-55
Received: Sep. 18, 2018;
Accepted: Oct. 5, 2018;
Published: Nov. 1, 2018
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Larisa Mikhailovna Somova, Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation
Irina Nikolaevna Lyapun, Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation
Elena Igorevna Drobot, Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation
Felix Nikolayevich Shubin, Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation
Natalya Gennadyevna Plekhova, Ministry of Health of Russia, Pacific Medical University, Vladivostok, Russian Federation
In a comparative aspect, the functional state of inflammation effector cells in animals infected with various plasmid types of Yersinia pseudotuberculosis was studied. The metabolic activity of peritoneal exudate cells has been investigated in an experimental infection caused by four plasmid types of Y. pseudotuberculosis: type 82+: 48+, containing two plasmids pVM 82 and pYV; type 82+: 48- containing single pVM 82 plasmid; type 48+: 82- containing single pYV plasmid; plasmid-free type 48-: 82-. The parameters of enzyme activity (ATP-ase, 5'-nucleotidase, lactate dehydrogenase, myeloperoxidase) and the level of nitric oxide metabolites were determined. The variability of the metabolic activity of the cells in the inflammatory focus (peritoneal exudate containing neutrophils and macrophages) in infected animals has been established. In response to infection with Y. pseudotuberculosis strain containing two plasmids pYV and pVM82, the production of the nitric oxide metabolites, rather than the active forms of oxygen, had the primary importance in providing the bactericidal potential of phagocytes, compared to animals infected with a strain containing a single pVM82 plasmid. It was concluded that a special biological effect associated with the pVM 82 plasmid available in the Far Eastern strains of the causative agent of epidemic pseudotuberculosis (Far Eastern scarlet-like fever) was involved in the provision of predominantly nitroxide-dependent bactericidal mechanisms of innate immunity cells in this infection.
Larisa Mikhailovna Somova,
Irina Nikolaevna Lyapun,
Elena Igorevna Drobot,
Felix Nikolayevich Shubin,
Natalya Gennadyevna Plekhova,
The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis, Biochemistry and Molecular Biology.
Vol. 3, No. 3,
2018, pp. 49-55.
Skurnik M., Peippo A., Ervelia E. (2000) Characterization of the O-antigen gene clusters of Y. pseudotuberculosis and the criptic O-gene cluster of Y. pestis shows that the plague bacillis is the most closely related to and has evolved from Y. pseudotuberculosis serotype O: 1b. Mol Microbiol 37: 316-330.
Somova L. M., Andryukov B. G., Plekhova N. G. (2015) The problem of yersiniosis in the modern world. Intern J. Appl Basic Res 12: 661-667.
Philip N. H., Brodsky I. E. (2012) Cell death programs in Yersinia immunity and pathogenesis. Front Cell Infect Microbiol 2 (149): 1-7.
Somov G. P., Pokrovsky V. I., Besednova N. N., Antonenko F. F. (2001) Pseudotuberculosis. M.: Medicine.
Shurygina I. A., Chesnokova M. V., Klimov V. T., Malov I. V., Maramovich A. S. (2003) Pseudotuberculosis. Novosibirsk: Science.
Somova L. M., Shubin F. N., Drobot E. I., Plekhova N. G., Lyapun I. N. (2016) Plasmid-associated virulence of Yersinia pseudotuberculosis and infectious process. J Microbiol Epidemiol Immunobiol 6: 74-84.
Brodsky I. E., Palm N. W., Sadanand S., Ryndak M. B., Sutterwala F. S. et al. (2010) Effector promotes virulence by preventing inflammasome recognition of the type III secretion system. Cell Host Microbe. 7 ( 5): 376-387.
Timchenko N. F., Adgamov R. R., Popov A. F., Psareva E. K., Sobyanin K. A., Gintsburg A. L., Ermplaeva S. A. (2016) East Scarlet-like fever caused by a Few related genotypes of Yersinia pseudotuberculosis. Emerging Infest Dis 22 (3): 503-506.
Cornelis G. R. (2010) The type III seсretion injectisome, a complex nanomachine for intracellular toxin delivery. Biol Chem 391 (7): 745-751.
Groves E. 1., Rittinger K., Amstutz M., Berry S., Holden D. W., Cornelis G. R., Caron E. (2010) Sequestering of Rac by the Yersinia effector YopO blocks Fc gamma receptor-mediated phagocytosis. J Biol Chem 285 (6): 4087-4098.
Naberezhnykh G. A., Sidorin E. V., Lapshina L. A., Reunov A. V., Solovyova T. F. (2006) Influence of cultivation conditions and virulence plasmids on the expression of immunoglobulin-binding proteins Yersinia pseudotuberculosis. Biochemistry 71 (11): 1577-1582.
Sever I. S. (1996) Effect of pVM82 Yersinia pseudotuberculosis on the activity of complement components and phagocytosis by human blood neutrophils. Mol Gen Microbiol Virol 1: 23-26.
Shurygina I. A., Malov A. V., Maramovich A. S., Klimov V. T. (2001) The effect of a plasmid with 82 MD molecular weight on the clinical and morphological manifestations of pseudotuberculosis. Sib Med J 26 (1): 48-53.
Dubrovina V. I., Golubinsky E. P., Borsuk G. I., Balakhonov S. V., Konovalova Zh. A. (1999) Features of Yersinia pseudotuberculosis phagocytosis with a different set of plasmids. Med Parasitol 4: 50-53.
Plekhova N. G., Somova L. M., Okhotina S. V., Drobot E. I., Goncharuk Yu. N. (2006) Metabolic activity of neutrophils in pseudotuberculous infection. J Microbiol Epidemiol Immunobiol 3: 43-47.
Navarini A. A., Lang K. S., Verschoor A., Recher M., Zinkernagel A. S. et al. (2009) Innate immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections. PNAS 106 (17): 7107-7112.
Loyda Z., Gossrau R., Shibler T. (1982) Histochemistry of enzymes: laboratory methods. Moscow: The World.
Schulz К., Kerber S., Kelm M. (1999) Reevalution of the Griess method for determining NO/NO2- in aqueous and protein-containing samples. Nitric Oxide 3 (3): 225-234.
Totolyan A. A., Freidlin I. S. (2000). Cells of the immune system. Saint-Petersburg: Science. 2000.
Kohlman J., Rem K. G. (2000) Visual Biochemistry. Moscow: The World.
Evans T. J., Buttery L. D. K., Carpenter A., Springall D. R., Polak J. M., Cohen J. (1996) Cytokine-treated human neutrophils contain inducible nitric oxide synthase that produces nitration of ingested bacteria. Cell Biol 93: 9553-9558.
Fang F. C., Vazquez-Torres A. (2002) Nitric oxide production by human macrophages: there’s NO doubt about it. Amer J Physiol 282 (5): 941-943.