Biochemistry and Molecular Biology

| Peer-Reviewed |

Assessment of Consciousness Energy Healing Treatment on Immune Biomarkers After Oral Administration of Herbomineral Formulation in Sprague Dawley Male Rats

Received: 22 October 2017    Accepted: 03 November 2017    Published: 11 December 2017
Views:       Downloads:

Share This Article

Abstract

Herbomineral formulations have increased in recognition and popularity due to their high safety and better therapeutic benefits than other conventional methods. The aim of the present study was to evaluate the immunomodulatory effect of the Trivedi Effect®- Energy of Consciousness Healing Treatment on a new proprietary herbomineral formulation in male Sprague Dawley (SD) rats. The test formulation contained essential ingredients like the herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The immunomodulatory effect of the Biofield Energy Treated and untreated test formulations was studied in SD rats to determine any alteration in humoral immune response (IgG and IgM), cellular immune response, hematology, lipid profile, hepatic enzymes, and the testosterone. The Biofield Energy Treated test formulation group (G3) showed significantly increased levels of IgM and IgG by 46.15% and 14.92%, respectively compared with the disease control group (G2). The cellular immune response (i.e. the ratio of CD4+ and CD8+) was decreased by 76.47% in the Biofield Energy Treated test formulation compared with the G2 group. The hematology parameters such as TLC, neutrophils, lymphocytes, and monocytes were significantly increased by 18.01%, 1.34%, 12.34%, and 0.67%, respectively in the Biofield Energy Treated test formulation group compared with the G2 group. The lipid profile parameters viz. total cholesterol, triglycerides, HDL, LDL, and VLDL were significantly decreased in the Biofield Energy Treated test formulation group (G3) by 8.73%, 10.96%, 6.26%, 8.60%, and 11.01%, respectively compared with the G2 group. Among the tested hepatic parameters, results showed decreased levels of SGOT (5.27%), SGPT (17.70%), ALP (0.26%), total protein (5.8%), albumin (5.47%), globulin (9.27%), and albumin/globulin ratio (1.34%) in the Biofield Energy Treated test formulation group compared with the G2 group. However, the Biofield Energy Treated test formulation group animals exhibited an increased level of testosterone by 39.8% compared with the G2 group. The overall results demonstrated that the Biofield Energy Treated test formulation showed a better immune response compared with the untreated test formulation. These experimental data suggest that the Trivedi Effect®- Energy of Consciousness Healing Treatment based herbomineral formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.

DOI 10.11648/j.bmb.20170206.17
Published in Biochemistry and Molecular Biology (Volume 2, Issue 6, November 2017)
Page(s) 110-119
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Biofield Energy Healing Treatment, Biofield Energy Healers, Herbomineral Formulation, Immune-Modulation, Cellular and Humoral Immune Response, Hepatic Biomarkers, Anti-Aging, Inflammatory Disease and Stress Management

References
[1] Thomson GE (2007) The Health Benefits of Traditional Chinese Plant Medicines: Weighing the Scientific Evidence: A Report for the Rural Industries Research and Development Corporation, RIRDC, Barton, Australia.
[2] Rishton GM (2008) Natural products as a robust source of new drugs and drug leads: Past successes and present day issues. Am J Cardiol 101: 43D-49D.
[3] Mukhtar M, Arshad M, Ahmad M, Pomerantz R, Wigdahl B, Parveen Z (2008) Antiviral potentials of medicinal plants. Virus Res 131: 111-120.
[4] Wang JZ, Mao XJ, Ito H, Shimura K (1991) Immunomodulatory activity of polysaccharide from Acanthopanex obovatus roots. Planta Med 57: 335-336.
[5] Lukác N, Massányi P (2007) Effects of trace elements on the immune system. Epidemiol Mikrobiol Imunol 56: 3-9.
[6] Galland L (1988) Magnesium and immune function: an overview. Magnesium 7: 290‐299.
[7] Wintergerst ES, Maggini S, Hornig DH (2007) Contribution of selected vitamins and trace elements to immune function. Ann Nutr Metab 51: 301‐323.
[8] Ziauddin M, Phansalkar N, Patki P, Diwanay S, Patwardhan B (1996) Studies on the immunomodulatory effects of ashwagandha. J Ethnopharmacol 50: 69-76.
[9] Singh N, Bhalla M, de Jager P, Gilca M (2011) An Overview on Ashwagandha: A Rasayana (Rejuvenator) of Ayurveda. Afr J Tradit Complement Altern Med 8: 208-213.
[10] Mager J, Moore D, Bendl D, Wong B, Rachlin K, Yount G (2007) Evaluating biofield treatments in a cell culture model of oxidative stress. Explore (NY) 3: 386-390.
[11] Jain S, Hammerschlag R, Mills P, Cohen L, Krieger R, Vieten C, Lutgendorf S (2015) Clinical studies of biofield therapies: Summary, methodological challenges, and recommendations. Glob Adv Health Med 4: 58-66.
[12] Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med 8: 703-717.
[13] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4: 141.
[14] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In vitro evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther 7: 253-257.
[15] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) In vitro evaluation of biofield treatment on Enterobacter cloacae: Impact on antimicrobial susceptibility and biotype. J Bacteriol Parasitol 6: 241.
[16] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) Evaluation of biofield modality on viral load of hepatitis B and C Viruses. J Antivir Antiretrovir 7: 83-88.
[17] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An impact of biofield treatment: Antimycobacterial susceptibility potential using BACTEC 460/MGIT-TB System. Mycobact Dis 5: 189.
[18] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Antimicrobial sensitivity, biochemical characteristics and biotyping of Staphylococcus saprophyticus: An impact of biofield energy treatment. J Women’s Health Care 4: 271.
[19] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of antibiogram, genotype and phylogenetic analysis of biofield treated Nocardia otitidis. Biol Syst Open Access 4: 143.
[20] Trivedi MK, Branton A, Trivedi D, Nayak G, Charan S, Jana S (2015) Phenotyping and 16S rDNA analysis after biofield treatment on Citrobacter braakii: A urinary pathogen. J Clin Med Genom 3: 129.
[21] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Spectroscopic characterization of chloramphenicol and tetracycline: An impact of biofield. Pharm Anal Acta 6: 395.
[22] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Spectroscopic characterization of biofield treated metronidazole and tinidazole. Med Chem 5: 340-344.
[23] Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, Jana S (2016) Determination of isotopic abundance of 2H, 13C, 18O, and 37Cl in biofield energy treated dichlorophenol isomers. Science Journal of Analytical Chemistry 4: 1-6.
[24] Trivedi MK, Branton A, Trivedi D, Shettigar H, Bairwa K, Jana S (2015) Fourier transform infrared and ultraviolet-visible spectroscopic characterization of biofield treated salicylic acid and sparfloxacin. Nat Prod Chem Res 3: 186.
[25] Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2016) Molecular analysis of biofield treated eggplant and watermelon crops. Adv Crop Sci Tech 4: 208.
[26] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Morphological characterization, quality, yield and DNA fingerprinting of biofield energy treated alphonso mango (Mangifera indica L.). Journal of Food and Nutrition Sciences 3: 245-250.
[27] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of plant growth, yield and yield attributes of biofield energy treated mustard (Brassica juncea) and chick pea (Cicer arietinum) seeds. Agriculture, Forestry and Fisheries 4: 291-295.
[28] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of plant growth regulator, immunity and DNA fingerprinting of biofield energy treated mustard seeds (Brassica juncea). Agriculture, Forestry and Fisheries 4: 269-274.
[29] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Jana S (2015) Characterization of physical and structural properties of aluminum carbide powder: Impact of biofield treatment. J Aeronaut Aerospace Eng 4: 142.
[30] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) Impact of biofield treatment on atomic and structural characteristics of barium titanate powder. Ind Eng Manage 4: 166.
[31] Trivedi MK, Patil S, Nayak G, Jana S, Latiyal O (2015) Influence of biofield treatment on physical, structural and spectral properties of boron nitride. J Material Sci Eng 4: 181.
[32] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) Characterization of physical and structural properties of brass powder after biofield treatment. J Powder Metall Min 4: 134.
[33] Ladics GS (2007) Primary immune response to sheep red blood cells (SRBC) as the conventional T-cell dependent antibody response (TDAR) test. J Immunotoxicol 4: 149-152.
[34] Madhuri S, Pandey G, Khanna A, Shrivastav AB (2012) Effect of some herbal drugs on haematological profiles of rats. IRJP 3: 158-160.
[35] Gautam M, Diwanay SS, Gairolac S, Shinde YS, Jadhav SS, Patwardhan BK (2004) Immune response modulation to DPT vaccine by aqueous extract of Withania somnifera in experimental system. Int Immunopharmacol 4: 841-849.
[36] Ziauddin M, N Phansalkar, P Patki, S Diwanay, B Patwardhan (1996) Studies on the immunomodulatory effects of Ashwagandha. J Ethnopharmacol 50: 69-76.
[37] Davis L, Kuttan G (2000) Effect of Withania somnifera on DMBA induced carcinogenesis. J Ethnopharmacol 75: 165-168.
[38] Andallu B, Radhika B (2000) Hypoglycemic, diuretic and hypocholesterolemic effect of winter cherry (Withania somnifera, Dunal) root. Indian J Exp Biol 38: 607-609.
[39] Bunglavan SJ, Garg AK, Dass RS, Shrivastava S (2014) Effect of supplementation of different levels of selenium as nanoparticles/sodium selenite on blood biochemical profile and humoral immunity in male wistar rats. Vet World 7: 1075-1081.
[40] Fox C, Ramsoomair D, Carter C (2001) Magnesium: Its proven and potential clinical significance. South Med J 94: 1195-1201.
[41] Payahoo L, Ostadrahimi A, Mobasseri M, Khaje Bishak Y, Farrin N, Asghari Jafarabadi M, Mahluji S (2013) Effects of zinc supplementation on the anthropometric measurements, lipid profiles and fasting blood glucose in the healthy obese adults. Adv Pharm Bull 3: 161-165.
[42] Torres-Duran PV, Ferreira-Hermosillo A, Juarez-Oropeza MA, Elias-Viñas D, Verdugo-Diaz L (2007) Effects of whole body exposure to extremely low frequency electromagnetic fields (ELF-EMF) on serum and liver lipid levels, in the rat. Lipids in Health and Disease 6: 31.
[43] Giannini EG, Testa R, Savarino V (2005) Liver enzyme alteration: A guide for clinicians. CMAJ 172: 367-379.
[44] Sabiba EP, Rasool M, Vedi M, Navaneethan D, Ravichander M, Parthasarathy P, Thella SR (2013) Hepatoprotective and antioxidant potential of Withania somnifera against paracetamol-induced liver damage in rats. Int J Pharm Pharm Sci 5: 648-651.
[45] Nangliya V, Sharma A, Yadav D, Sunder S, Nijhawan S, Mishra S (2015) Study of trace elements in liver cirrhosis patients and their role in prognosis of disease. Biol Trace Elem Res 165: 35-40.
[46] Ambiye VR, Langade D, Dongre S, Aptikar P, Kulkarni M, Dongre A (2013) Clinical evaluation of the spermatogenic activity of the root extract of Ashwagandha (Withania somnifera) in oligospermic males: A Pilot Study. Evid Based Complement Alternat Med 2013: 571420.
[47] Shafiei Neek, Gaeini AA, Choobineh S (2011) Effect of zinc and selenium supplementation on serum testosterone and plasma lactate in cyclist after an exhaustive exercise bout. Biol Trace Elem Res 144: 454-462.
[48] Cinar V, Polat Y, Baltaci AK, Mogulkoc R (2011) Effects of magnesium supplementation on testosterone levels of athletes and sedentary subjects at rest and after exhaustion. Biol Trace Elem Res 140: 18-23.
[49] Spallholz JE, Stewart JR (1989) Advances in the role of minerals in immunobiology. Biol Trace Elem Res 19: 129-151.
Author Information
  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India

Cite This Article
  • APA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, et al. (2017). Assessment of Consciousness Energy Healing Treatment on Immune Biomarkers After Oral Administration of Herbomineral Formulation in Sprague Dawley Male Rats. Biochemistry and Molecular Biology, 2(6), 110-119. https://doi.org/10.11648/j.bmb.20170206.17

    Copy | Download

    ACS Style

    Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Aileen Carol Lee, et al. Assessment of Consciousness Energy Healing Treatment on Immune Biomarkers After Oral Administration of Herbomineral Formulation in Sprague Dawley Male Rats. Biochem. Mol. Biol. 2017, 2(6), 110-119. doi: 10.11648/j.bmb.20170206.17

    Copy | Download

    AMA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, et al. Assessment of Consciousness Energy Healing Treatment on Immune Biomarkers After Oral Administration of Herbomineral Formulation in Sprague Dawley Male Rats. Biochem Mol Biol. 2017;2(6):110-119. doi: 10.11648/j.bmb.20170206.17

    Copy | Download

  • @article{10.11648/j.bmb.20170206.17,
      author = {Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Aileen Carol Lee and Aksana Hancharuk and Carola Marina Sand and Debra Jane Schnitzer and Rudina Thanasi and Eileen Mary Meagher and Faith Ann Pyka and Gary Richard Gerber and Johanna Catharina Stromsnas and Judith Marian Shapiro and Laura Nelson Streicher and Lorraine Marie Hachfeld and Matthew Charles Hornung and Patricia M. Rowe and Sally Jean Henderson and Sheila Maureen Benson and Shirley Theresa Holmlund and Stephen P. Salters and Mayank Gangwar and Snehasis Jana},
      title = {Assessment of Consciousness Energy Healing Treatment on Immune Biomarkers After Oral Administration of Herbomineral Formulation in Sprague Dawley Male Rats},
      journal = {Biochemistry and Molecular Biology},
      volume = {2},
      number = {6},
      pages = {110-119},
      doi = {10.11648/j.bmb.20170206.17},
      url = {https://doi.org/10.11648/j.bmb.20170206.17},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.bmb.20170206.17},
      abstract = {Herbomineral formulations have increased in recognition and popularity due to their high safety and better therapeutic benefits than other conventional methods. The aim of the present study was to evaluate the immunomodulatory effect of the Trivedi Effect®- Energy of Consciousness Healing Treatment on a new proprietary herbomineral formulation in male Sprague Dawley (SD) rats. The test formulation contained essential ingredients like the herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The immunomodulatory effect of the Biofield Energy Treated and untreated test formulations was studied in SD rats to determine any alteration in humoral immune response (IgG and IgM), cellular immune response, hematology, lipid profile, hepatic enzymes, and the testosterone. The Biofield Energy Treated test formulation group (G3) showed significantly increased levels of IgM and IgG by 46.15% and 14.92%, respectively compared with the disease control group (G2). The cellular immune response (i.e. the ratio of CD4+ and CD8+) was decreased by 76.47% in the Biofield Energy Treated test formulation compared with the G2 group. The hematology parameters such as TLC, neutrophils, lymphocytes, and monocytes were significantly increased by 18.01%, 1.34%, 12.34%, and 0.67%, respectively in the Biofield Energy Treated test formulation group compared with the G2 group. The lipid profile parameters viz. total cholesterol, triglycerides, HDL, LDL, and VLDL were significantly decreased in the Biofield Energy Treated test formulation group (G3) by 8.73%, 10.96%, 6.26%, 8.60%, and 11.01%, respectively compared with the G2 group. Among the tested hepatic parameters, results showed decreased levels of SGOT (5.27%), SGPT (17.70%), ALP (0.26%), total protein (5.8%), albumin (5.47%), globulin (9.27%), and albumin/globulin ratio (1.34%) in the Biofield Energy Treated test formulation group compared with the G2 group. However, the Biofield Energy Treated test formulation group animals exhibited an increased level of testosterone by 39.8% compared with the G2 group. The overall results demonstrated that the Biofield Energy Treated test formulation showed a better immune response compared with the untreated test formulation. These experimental data suggest that the Trivedi Effect®- Energy of Consciousness Healing Treatment based herbomineral formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.},
     year = {2017}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Assessment of Consciousness Energy Healing Treatment on Immune Biomarkers After Oral Administration of Herbomineral Formulation in Sprague Dawley Male Rats
    AU  - Mahendra Kumar Trivedi
    AU  - Alice Branton
    AU  - Dahryn Trivedi
    AU  - Gopal Nayak
    AU  - Aileen Carol Lee
    AU  - Aksana Hancharuk
    AU  - Carola Marina Sand
    AU  - Debra Jane Schnitzer
    AU  - Rudina Thanasi
    AU  - Eileen Mary Meagher
    AU  - Faith Ann Pyka
    AU  - Gary Richard Gerber
    AU  - Johanna Catharina Stromsnas
    AU  - Judith Marian Shapiro
    AU  - Laura Nelson Streicher
    AU  - Lorraine Marie Hachfeld
    AU  - Matthew Charles Hornung
    AU  - Patricia M. Rowe
    AU  - Sally Jean Henderson
    AU  - Sheila Maureen Benson
    AU  - Shirley Theresa Holmlund
    AU  - Stephen P. Salters
    AU  - Mayank Gangwar
    AU  - Snehasis Jana
    Y1  - 2017/12/11
    PY  - 2017
    N1  - https://doi.org/10.11648/j.bmb.20170206.17
    DO  - 10.11648/j.bmb.20170206.17
    T2  - Biochemistry and Molecular Biology
    JF  - Biochemistry and Molecular Biology
    JO  - Biochemistry and Molecular Biology
    SP  - 110
    EP  - 119
    PB  - Science Publishing Group
    SN  - 2575-5048
    UR  - https://doi.org/10.11648/j.bmb.20170206.17
    AB  - Herbomineral formulations have increased in recognition and popularity due to their high safety and better therapeutic benefits than other conventional methods. The aim of the present study was to evaluate the immunomodulatory effect of the Trivedi Effect®- Energy of Consciousness Healing Treatment on a new proprietary herbomineral formulation in male Sprague Dawley (SD) rats. The test formulation contained essential ingredients like the herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The immunomodulatory effect of the Biofield Energy Treated and untreated test formulations was studied in SD rats to determine any alteration in humoral immune response (IgG and IgM), cellular immune response, hematology, lipid profile, hepatic enzymes, and the testosterone. The Biofield Energy Treated test formulation group (G3) showed significantly increased levels of IgM and IgG by 46.15% and 14.92%, respectively compared with the disease control group (G2). The cellular immune response (i.e. the ratio of CD4+ and CD8+) was decreased by 76.47% in the Biofield Energy Treated test formulation compared with the G2 group. The hematology parameters such as TLC, neutrophils, lymphocytes, and monocytes were significantly increased by 18.01%, 1.34%, 12.34%, and 0.67%, respectively in the Biofield Energy Treated test formulation group compared with the G2 group. The lipid profile parameters viz. total cholesterol, triglycerides, HDL, LDL, and VLDL were significantly decreased in the Biofield Energy Treated test formulation group (G3) by 8.73%, 10.96%, 6.26%, 8.60%, and 11.01%, respectively compared with the G2 group. Among the tested hepatic parameters, results showed decreased levels of SGOT (5.27%), SGPT (17.70%), ALP (0.26%), total protein (5.8%), albumin (5.47%), globulin (9.27%), and albumin/globulin ratio (1.34%) in the Biofield Energy Treated test formulation group compared with the G2 group. However, the Biofield Energy Treated test formulation group animals exhibited an increased level of testosterone by 39.8% compared with the G2 group. The overall results demonstrated that the Biofield Energy Treated test formulation showed a better immune response compared with the untreated test formulation. These experimental data suggest that the Trivedi Effect®- Energy of Consciousness Healing Treatment based herbomineral formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.
    VL  - 2
    IS  - 6
    ER  - 

    Copy | Download

  • Sections