Distinct Role of Transforming Growth Factor-Beta 1 and Fibroblast Growth Factors in Human Amelobastoma Epithelial Cell Proliferation

Yuko Matsuda; Naoko Kamogashira; Yuji Hatakeyama; Toshinari Mikami; Kazuki Nakashima; Junko Hatakeyama; Sachio Tamaoki; Yoshihiko Sawa; Hiroyuki Ishikawa

doi:doi:10.11648/j.bmb.20170201.11

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Distinct Role of Transforming Growth Factor-Beta 1 and Fibroblast Growth Factors in Human Amelobastoma Epithelial Cell Proliferation

Yuko Matsuda, Naoko Kamogashira, Yuji Hatakeyama, Toshinari Mikami, Kazuki Nakashima, Junko Hatakeyama, Sachio Tamaoki, Yoshihiko Sawa, Hiroyuki Ishikawa

Received: 30 January 2017 Accepted: 22 February 2017 Published: 9 March 2017

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Abstract

Ameloblastoma is a locally invasive benign epithelial odontogenic tumor and its histopathological structures are similar to the enamel organ. Although various studies have investigated cell proliferation in ameloblastoma to elucidate the biological behavior and clinicopathological mechanisms, it remains poorly understood. The studies on the development of the enamel organ reports that FGF-9, -10, and TGF-β1 are strongly involved in dental epithelial cell differentiation and cell proliferation. In this study, we attempt to evaluate the effect of these growth factors on ameloblastoma cells. Both collagen-coated and normal plastic cell culture plate cell growth curves were steeper in the presence of growth supplement than in the absence of growth supplement. The presence of TGF-β1 at each dose (1 to 10 ng/ml), however, suppressed the number of cells cultured on the collagen-coated plate but made no significant difference on the normal plastic plate. The number of cells was increased in the presence of FGF-10 at 100 ng/ml, but not in the presence of FGF-9 after 48 h culture. These results suggest that FGF-10 and TGF-β1 play distinct roles in the cell proliferation of human ameloblastoma cells.

DOI	10.11648/j.bmb.20170201.11
Published in	Biochemistry and Molecular Biology (Volume 2, Issue 1, January 2017)
Page(s)	1-5
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Fibroblast Growth Factor (FGF), Transforming Growth Factor-Beta 1 (TGF-β1), Dental Epithelial Cell, Cell Proliferation

References

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APA Style

Yuko Matsuda, Naoko Kamogashira, Yuji Hatakeyama, Toshinari Mikami, Kazuki Nakashima, et al. (2017). Distinct Role of Transforming Growth Factor-Beta 1 and Fibroblast Growth Factors in Human Amelobastoma Epithelial Cell Proliferation. Biochemistry and Molecular Biology, 2(1), 1-5. https://doi.org/10.11648/j.bmb.20170201.11

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ACS Style

Yuko Matsuda; Naoko Kamogashira; Yuji Hatakeyama; Toshinari Mikami; Kazuki Nakashima, et al. Distinct Role of Transforming Growth Factor-Beta 1 and Fibroblast Growth Factors in Human Amelobastoma Epithelial Cell Proliferation. Biochem. Mol. Biol. 2017, 2(1), 1-5. doi: 10.11648/j.bmb.20170201.11

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AMA Style

Yuko Matsuda, Naoko Kamogashira, Yuji Hatakeyama, Toshinari Mikami, Kazuki Nakashima, et al. Distinct Role of Transforming Growth Factor-Beta 1 and Fibroblast Growth Factors in Human Amelobastoma Epithelial Cell Proliferation. Biochem Mol Biol. 2017;2(1):1-5. doi: 10.11648/j.bmb.20170201.11

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@article{10.11648/j.bmb.20170201.11,
  author = {Yuko Matsuda and Naoko Kamogashira and Yuji Hatakeyama and Toshinari Mikami and Kazuki Nakashima and Junko Hatakeyama and Sachio Tamaoki and Yoshihiko Sawa and Hiroyuki Ishikawa},
  title = {Distinct Role of Transforming Growth Factor-Beta 1 and Fibroblast Growth Factors in Human Amelobastoma Epithelial Cell Proliferation},
  journal = {Biochemistry and Molecular Biology},
  volume = {2},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.bmb.20170201.11},
  url = {https://doi.org/10.11648/j.bmb.20170201.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20170201.11},
  abstract = {Ameloblastoma is a locally invasive benign epithelial odontogenic tumor and its histopathological structures are similar to the enamel organ. Although various studies have investigated cell proliferation in ameloblastoma to elucidate the biological behavior and clinicopathological mechanisms, it remains poorly understood. The studies on the development of the enamel organ reports that FGF-9, -10, and TGF-β1 are strongly involved in dental epithelial cell differentiation and cell proliferation. In this study, we attempt to evaluate the effect of these growth factors on ameloblastoma cells. Both collagen-coated and normal plastic cell culture plate cell growth curves were steeper in the presence of growth supplement than in the absence of growth supplement. The presence of TGF-β1 at each dose (1 to 10 ng/ml), however, suppressed the number of cells cultured on the collagen-coated plate but made no significant difference on the normal plastic plate. The number of cells was increased in the presence of FGF-10 at 100 ng/ml, but not in the presence of FGF-9 after 48 h culture. These results suggest that FGF-10 and TGF-β1 play distinct roles in the cell proliferation of human ameloblastoma cells.},
 year = {2017}
}

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TY  - JOUR
T1  - Distinct Role of Transforming Growth Factor-Beta 1 and Fibroblast Growth Factors in Human Amelobastoma Epithelial Cell Proliferation
AU  - Yuko Matsuda
AU  - Naoko Kamogashira
AU  - Yuji Hatakeyama
AU  - Toshinari Mikami
AU  - Kazuki Nakashima
AU  - Junko Hatakeyama
AU  - Sachio Tamaoki
AU  - Yoshihiko Sawa
AU  - Hiroyuki Ishikawa
Y1  - 2017/03/09
PY  - 2017
N1  - https://doi.org/10.11648/j.bmb.20170201.11
DO  - 10.11648/j.bmb.20170201.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 1
EP  - 5
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20170201.11
AB  - Ameloblastoma is a locally invasive benign epithelial odontogenic tumor and its histopathological structures are similar to the enamel organ. Although various studies have investigated cell proliferation in ameloblastoma to elucidate the biological behavior and clinicopathological mechanisms, it remains poorly understood. The studies on the development of the enamel organ reports that FGF-9, -10, and TGF-β1 are strongly involved in dental epithelial cell differentiation and cell proliferation. In this study, we attempt to evaluate the effect of these growth factors on ameloblastoma cells. Both collagen-coated and normal plastic cell culture plate cell growth curves were steeper in the presence of growth supplement than in the absence of growth supplement. The presence of TGF-β1 at each dose (1 to 10 ng/ml), however, suppressed the number of cells cultured on the collagen-coated plate but made no significant difference on the normal plastic plate. The number of cells was increased in the presence of FGF-10 at 100 ng/ml, but not in the presence of FGF-9 after 48 h culture. These results suggest that FGF-10 and TGF-β1 play distinct roles in the cell proliferation of human ameloblastoma cells.
VL  - 2
IS  - 1
ER  -

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Author Information

Yuko Matsuda

Section of Orthodontics, Department of Oral Growth and Development, Fukuoka Dental College, Fukuoka, Japan
Naoko Kamogashira

Section of Orthodontics, Department of Oral Growth and Development, Fukuoka Dental College, Fukuoka, Japan
Yuji Hatakeyama

Section of Functional Structure, Department of Morphological Biology, Fukuoka Dental College, Fukuoka, Japan
Toshinari Mikami

Division of Anatomical and Cellular Pathology, Department of Pathology, Iwate Medical University, Iwate, Japan
Kazuki Nakashima

Section of Orthodontics, Department of Oral Growth and Development, Fukuoka Dental College, Fukuoka, Japan
Junko Hatakeyama

Section of Operative Dentistry and Endodontology, Department of Odontology, Fukuoka Dental College, Fukuoka, Japan
Sachio Tamaoki

Section of Orthodontics, Department of Oral Growth and Development, Fukuoka Dental College, Fukuoka, Japan
Yoshihiko Sawa

Section of Functional Structure, Department of Morphological Biology, Fukuoka Dental College, Fukuoka, Japan
Hiroyuki Ishikawa

Section of Orthodontics, Department of Oral Growth and Development, Fukuoka Dental College, Fukuoka, Japan

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APA Style

Yuko Matsuda, Naoko Kamogashira, Yuji Hatakeyama, Toshinari Mikami, Kazuki Nakashima, et al. (2017). Distinct Role of Transforming Growth Factor-Beta 1 and Fibroblast Growth Factors in Human Amelobastoma Epithelial Cell Proliferation. Biochemistry and Molecular Biology, 2(1), 1-5. https://doi.org/10.11648/j.bmb.20170201.11

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ACS Style

Yuko Matsuda; Naoko Kamogashira; Yuji Hatakeyama; Toshinari Mikami; Kazuki Nakashima, et al. Distinct Role of Transforming Growth Factor-Beta 1 and Fibroblast Growth Factors in Human Amelobastoma Epithelial Cell Proliferation. Biochem. Mol. Biol. 2017, 2(1), 1-5. doi: 10.11648/j.bmb.20170201.11

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AMA Style

Yuko Matsuda, Naoko Kamogashira, Yuji Hatakeyama, Toshinari Mikami, Kazuki Nakashima, et al. Distinct Role of Transforming Growth Factor-Beta 1 and Fibroblast Growth Factors in Human Amelobastoma Epithelial Cell Proliferation. Biochem Mol Biol. 2017;2(1):1-5. doi: 10.11648/j.bmb.20170201.11

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@article{10.11648/j.bmb.20170201.11,
  author = {Yuko Matsuda and Naoko Kamogashira and Yuji Hatakeyama and Toshinari Mikami and Kazuki Nakashima and Junko Hatakeyama and Sachio Tamaoki and Yoshihiko Sawa and Hiroyuki Ishikawa},
  title = {Distinct Role of Transforming Growth Factor-Beta 1 and Fibroblast Growth Factors in Human Amelobastoma Epithelial Cell Proliferation},
  journal = {Biochemistry and Molecular Biology},
  volume = {2},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.bmb.20170201.11},
  url = {https://doi.org/10.11648/j.bmb.20170201.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20170201.11},
  abstract = {Ameloblastoma is a locally invasive benign epithelial odontogenic tumor and its histopathological structures are similar to the enamel organ. Although various studies have investigated cell proliferation in ameloblastoma to elucidate the biological behavior and clinicopathological mechanisms, it remains poorly understood. The studies on the development of the enamel organ reports that FGF-9, -10, and TGF-β1 are strongly involved in dental epithelial cell differentiation and cell proliferation. In this study, we attempt to evaluate the effect of these growth factors on ameloblastoma cells. Both collagen-coated and normal plastic cell culture plate cell growth curves were steeper in the presence of growth supplement than in the absence of growth supplement. The presence of TGF-β1 at each dose (1 to 10 ng/ml), however, suppressed the number of cells cultured on the collagen-coated plate but made no significant difference on the normal plastic plate. The number of cells was increased in the presence of FGF-10 at 100 ng/ml, but not in the presence of FGF-9 after 48 h culture. These results suggest that FGF-10 and TGF-β1 play distinct roles in the cell proliferation of human ameloblastoma cells.},
 year = {2017}
}

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TY  - JOUR
T1  - Distinct Role of Transforming Growth Factor-Beta 1 and Fibroblast Growth Factors in Human Amelobastoma Epithelial Cell Proliferation
AU  - Yuko Matsuda
AU  - Naoko Kamogashira
AU  - Yuji Hatakeyama
AU  - Toshinari Mikami
AU  - Kazuki Nakashima
AU  - Junko Hatakeyama
AU  - Sachio Tamaoki
AU  - Yoshihiko Sawa
AU  - Hiroyuki Ishikawa
Y1  - 2017/03/09
PY  - 2017
N1  - https://doi.org/10.11648/j.bmb.20170201.11
DO  - 10.11648/j.bmb.20170201.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 1
EP  - 5
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20170201.11
AB  - Ameloblastoma is a locally invasive benign epithelial odontogenic tumor and its histopathological structures are similar to the enamel organ. Although various studies have investigated cell proliferation in ameloblastoma to elucidate the biological behavior and clinicopathological mechanisms, it remains poorly understood. The studies on the development of the enamel organ reports that FGF-9, -10, and TGF-β1 are strongly involved in dental epithelial cell differentiation and cell proliferation. In this study, we attempt to evaluate the effect of these growth factors on ameloblastoma cells. Both collagen-coated and normal plastic cell culture plate cell growth curves were steeper in the presence of growth supplement than in the absence of growth supplement. The presence of TGF-β1 at each dose (1 to 10 ng/ml), however, suppressed the number of cells cultured on the collagen-coated plate but made no significant difference on the normal plastic plate. The number of cells was increased in the presence of FGF-10 at 100 ng/ml, but not in the presence of FGF-9 after 48 h culture. These results suggest that FGF-10 and TGF-β1 play distinct roles in the cell proliferation of human ameloblastoma cells.
VL  - 2
IS  - 1
ER  -

Copy | Download