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Pre-analytical Phase in Hemostasis: The Main Anomalies and Means to Correct Them

Received: 21 August 2019    Accepted: 6 November 2019    Published: 21 November 2019
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Abstract

Pre-analytical errors still represent nearly 70% of all errors occurring in the laboratory, constituting a danger, a waste of time and an additional cost to the patient. The control of the different components of the pre-analytical step is important for the validity of the hemostasis exploration tests. The purpose of our work is to identify the main anomalies of the pre-analytical phase in hemostasis and to propose the means to correct them. We conducted a prospective and descriptive study on the pre-analytical phase of hemostasis. It was in the form of a survey, identifying the main errors related to this phase. It was performed at the Hematology laboratory of the Avicenna Military Hospital of Marrakech and spread over a period of 4 weeks. Our investigation took place at the hemostasis room, which received the collection tubes from the various hospital departments and the blood drawing room (for non-hospitalized patients). The hemostasis room received 400 prescription cards and their corresponding tubes. The parameters related to the prescription file: full name and gender of the patients, were mentioned on all the cards received and they were in conformity with those marked on the corresponding tube. The age of the patients and their clinical and therapeutic informations were mentioned in 73% and 13% of the exam requests, respectively. For the pre-analytical hemostasis parameters related to the blood collection: 63% of samples were taken at the laboratory's blood drawing room, while 37% came from the various hospital departments. Time of the realization of the samples was not mentioned on the cards or on the labels of the tubes. The anticoagulant used for all samples was sodium citrate at a concentration of 3.8%. The filling of the tubes was noncompliant in 22.25%. Registration and triage of the tubes systems were manual. Centrifugation was carried out at a rotation speed of 5000 G for 5 minutes and at a temperature set at 22°C. Hemolyzed samples accounted for 3% of the tubes.

Published in American Journal of Laboratory Medicine (Volume 4, Issue 6)
DOI 10.11648/j.ajlm.20190406.14
Page(s) 105-110
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Hemostasis, Pre-analytical Phase, Sample

References
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    Khayati Siham, Mouayche Ikhlas, Bahri Raihane, Ait Si Ali Zineb, Yahyaoui Hicham, et al. (2019). Pre-analytical Phase in Hemostasis: The Main Anomalies and Means to Correct Them. American Journal of Laboratory Medicine, 4(6), 105-110. https://doi.org/10.11648/j.ajlm.20190406.14

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    Khayati Siham; Mouayche Ikhlas; Bahri Raihane; Ait Si Ali Zineb; Yahyaoui Hicham, et al. Pre-analytical Phase in Hemostasis: The Main Anomalies and Means to Correct Them. Am. J. Lab. Med. 2019, 4(6), 105-110. doi: 10.11648/j.ajlm.20190406.14

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    AMA Style

    Khayati Siham, Mouayche Ikhlas, Bahri Raihane, Ait Si Ali Zineb, Yahyaoui Hicham, et al. Pre-analytical Phase in Hemostasis: The Main Anomalies and Means to Correct Them. Am J Lab Med. 2019;4(6):105-110. doi: 10.11648/j.ajlm.20190406.14

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  • @article{10.11648/j.ajlm.20190406.14,
      author = {Khayati Siham and Mouayche Ikhlas and Bahri Raihane and Ait Si Ali Zineb and Yahyaoui Hicham and Ait Ameur Mustapha and Chakour Mohammed},
      title = {Pre-analytical Phase in Hemostasis: The Main Anomalies and Means to Correct Them},
      journal = {American Journal of Laboratory Medicine},
      volume = {4},
      number = {6},
      pages = {105-110},
      doi = {10.11648/j.ajlm.20190406.14},
      url = {https://doi.org/10.11648/j.ajlm.20190406.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20190406.14},
      abstract = {Pre-analytical errors still represent nearly 70% of all errors occurring in the laboratory, constituting a danger, a waste of time and an additional cost to the patient. The control of the different components of the pre-analytical step is important for the validity of the hemostasis exploration tests. The purpose of our work is to identify the main anomalies of the pre-analytical phase in hemostasis and to propose the means to correct them. We conducted a prospective and descriptive study on the pre-analytical phase of hemostasis. It was in the form of a survey, identifying the main errors related to this phase. It was performed at the Hematology laboratory of the Avicenna Military Hospital of Marrakech and spread over a period of 4 weeks. Our investigation took place at the hemostasis room, which received the collection tubes from the various hospital departments and the blood drawing room (for non-hospitalized patients). The hemostasis room received 400 prescription cards and their corresponding tubes. The parameters related to the prescription file: full name and gender of the patients, were mentioned on all the cards received and they were in conformity with those marked on the corresponding tube. The age of the patients and their clinical and therapeutic informations were mentioned in 73% and 13% of the exam requests, respectively. For the pre-analytical hemostasis parameters related to the blood collection: 63% of samples were taken at the laboratory's blood drawing room, while 37% came from the various hospital departments. Time of the realization of the samples was not mentioned on the cards or on the labels of the tubes. The anticoagulant used for all samples was sodium citrate at a concentration of 3.8%. The filling of the tubes was noncompliant in 22.25%. Registration and triage of the tubes systems were manual. Centrifugation was carried out at a rotation speed of 5000 G for 5 minutes and at a temperature set at 22°C. Hemolyzed samples accounted for 3% of the tubes.},
     year = {2019}
    }
    

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    T1  - Pre-analytical Phase in Hemostasis: The Main Anomalies and Means to Correct Them
    AU  - Khayati Siham
    AU  - Mouayche Ikhlas
    AU  - Bahri Raihane
    AU  - Ait Si Ali Zineb
    AU  - Yahyaoui Hicham
    AU  - Ait Ameur Mustapha
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    DO  - 10.11648/j.ajlm.20190406.14
    T2  - American Journal of Laboratory Medicine
    JF  - American Journal of Laboratory Medicine
    JO  - American Journal of Laboratory Medicine
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    EP  - 110
    PB  - Science Publishing Group
    SN  - 2575-386X
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    AB  - Pre-analytical errors still represent nearly 70% of all errors occurring in the laboratory, constituting a danger, a waste of time and an additional cost to the patient. The control of the different components of the pre-analytical step is important for the validity of the hemostasis exploration tests. The purpose of our work is to identify the main anomalies of the pre-analytical phase in hemostasis and to propose the means to correct them. We conducted a prospective and descriptive study on the pre-analytical phase of hemostasis. It was in the form of a survey, identifying the main errors related to this phase. It was performed at the Hematology laboratory of the Avicenna Military Hospital of Marrakech and spread over a period of 4 weeks. Our investigation took place at the hemostasis room, which received the collection tubes from the various hospital departments and the blood drawing room (for non-hospitalized patients). The hemostasis room received 400 prescription cards and their corresponding tubes. The parameters related to the prescription file: full name and gender of the patients, were mentioned on all the cards received and they were in conformity with those marked on the corresponding tube. The age of the patients and their clinical and therapeutic informations were mentioned in 73% and 13% of the exam requests, respectively. For the pre-analytical hemostasis parameters related to the blood collection: 63% of samples were taken at the laboratory's blood drawing room, while 37% came from the various hospital departments. Time of the realization of the samples was not mentioned on the cards or on the labels of the tubes. The anticoagulant used for all samples was sodium citrate at a concentration of 3.8%. The filling of the tubes was noncompliant in 22.25%. Registration and triage of the tubes systems were manual. Centrifugation was carried out at a rotation speed of 5000 G for 5 minutes and at a temperature set at 22°C. Hemolyzed samples accounted for 3% of the tubes.
    VL  - 4
    IS  - 6
    ER  - 

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Author Information
  • Laboratory of Hematology, Avicenna Military Hospital, Mohammed VI UHC, Marrakech, Morocco

  • Laboratory of Hematology, Avicenna Military Hospital, Mohammed VI UHC, Marrakech, Morocco

  • Laboratory of Hematology, Avicenna Military Hospital, Mohammed VI UHC, Marrakech, Morocco

  • Laboratory of Hematology, Avicenna Military Hospital, Mohammed VI UHC, Marrakech, Morocco

  • Laboratory of Hematology, Avicenna Military Hospital, Mohammed VI UHC, Marrakech, Morocco

  • Laboratory of Hematology, Avicenna Military Hospital, Mohammed VI UHC, Marrakech, Morocco

  • Laboratory of Hematology, Avicenna Military Hospital, Mohammed VI UHC, Marrakech, Morocco

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