| Peer-Reviewed

An in Vitro Study of Biofield Energy Healing Based Herbomineral Formulation for Skin Protection

Received: 31 March 2017    Accepted: 10 April 2017    Published: 2 May 2017
Views:       Downloads:
Abstract

The current research work deals with the impact of the Consciousness Energy Healing Treatment (The Trivedi Effect®) based herbomineral test formulation and cell medium (DMEM) against various skin health parameters. The test formulation is the combination of minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts, i.e. one part received the Biofield Energy Treatment by Eileen Mary Meagher and was labeled as the Biofield Treated (BT) samples, while other represented was defined as the untreated test (UT) samples. The MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability. Cell proliferation assay using BrdU method showed an improved proliferation rate by 343.23%, 316.13%, and 512.75% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 µg/mL, compared with the untreated group. The collagen level was significantly increased by 15.70% and 13.52% at 2.5 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The level of elastin was increased by 47.0% and 64.1% at 2.5 and 1.25 µg/mL, respectively in the BT-DMEM + UT-Test formulation, group compared with the untreated group. Hyaluronic acid synthesis was increased by 4.37%, 41.47%, and 19.74% at 2.5, 1.25, and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation group with respect to the untreated group. Melanin synthesis was significantly inhibited by 8.83%, 5.78%, and 12.68% at 0.0125, 0.0625, and 0.125 µg/mL, respectively in the UT-DMEM + BT-Test formulation group compared with the untreated group. Anti-wrinkling effect showed an improved cell viability by 4.32% and 2.47% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT- Test formulation groups, respectively in HFF-1 cells compared with the untreated group. Wound healing activity using scratch assay exhibited improved a healing rate up to 6% in the HFF-1 and HaCaT cells lines in the Biofield Energy Treated Test formulation. In conclusion, results suggest that The Trivedi Effect®- Consciousness Energy Healing Treatment based test formulation and medium have the ability to improve the skin health and can be used as skincare products cosmetics for anti-ageing, anti-wrinkling action and for the treatment of many skin diseases such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, etc.

Published in American Journal of Laboratory Medicine (Volume 2, Issue 2)
DOI 10.11648/j.ajlm.20170202.11
Page(s) 13-23
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Consciousness Energy Healing Treatment, Scratch Assay, Extracellular Matrix, Hyaluronic Acid, Tetrahydrocurcumin

References
[1] Bahmani M, Sarrafchi A, Shirzad H, Rafieian-Kopaei M (2016) Autism: Pathophysiology and promising herbal remedies. Curr Pharm Des 22(3): 277-85.
[2] Nasri H, Shirzad H (2013) Toxicity and safety of medicinal plants. J Herb Med Plarmacol 2(2): 21-22.
[3] Sewell RDE, Rafieian-Kopaei M (2014) The history and ups and downs of herbal medicine usage. J Herb Med Pharmacol 3(1): 1-3.
[4] Davis SC, Perez R (2009) Cosmeceuticals and natural products: Wound healing. Clin Dermatol 27: 502-6.
[5] Goyal RK (2005) Investigation of cellular and molecular mechanisms for anti-diabetic drugs with special reference to Unani and Ayurvedic herbal medicines. In: traditional system of medicine, Abdin, M. Z. and Y. P. Abrol (Eds.). Narosa Publishing House, New Delhi.
[6] WHO (1993) Research Guideline for Evaluating the Safety and Efficacy of Herbal Medicines. World Health Organization, Manila, Philippines.
[7] Gao XH, Zhang L, Wei H, Chen HD (2008) Efficacy and safety of innovative cosmeceuticals. Clin Dermatol 26: 367-74.
[8] Fabricant DS, Farnsworth NR (2001) The value of plants used in traditional medicine for drug discovery. Environ Health Perspect 109: 69-75.
[9] Tabassum N, Hamdani M (2014) Plants used to treat skin diseases. Pharmacogn Rev 8: 52-60.
[10] Langton AK, Sherratt MJ, Griffiths CEM and Watson REB (2010) A new wrinkle on old skin: The role of elastic fibres in skin ageing. Int J Cosmet Sci 32: 330-339.
[11] Warren R, Gartstein V, Kligman AM, Montagna W, Allendorf RA and Ridder GM (1991) Age, sunlight, and facial skin: A histologic and quantitative study. J Am Acad Dermatol 25: 751-760.
[12] Park K (2015) Role of micronutrients in skin health and function. Biomol Ther (Seoul) 23: 207-217.
[13] Hashim P (2011) Centella asiatica in food and beverage applications and its potential antioxidant and neuroprotective effect. Int Food Res J 18: 1215-1222.
[14] Boyera N, Galey I, Bernard BA (1998) Effect of vitamin C and its derivatives on collagen synthesis and cross-linking by normal human fibroblasts. Int J Cosmet Sci 20: 151-158.
[15] Gupta SC, Patchva S, Koh W, Aggarwal BB (2012) Discovery of curcumin, a component of golden spice, and its miraculous biological activities. Clin Exp Pharmacol Physiol 39: 283-299.
[16] Majeed M, Badmaev V, Uma S, Rajenderan JR (1995) Curcuminoids: Antioxidant Phytonutrients, Nutreiscience publishers New Jersey 1-24.
[17] Sugiyama Y, Kawakishi S, Osawa T (1996) Involvement of the β-diketone moiety in the antioxidant mechanism of tetrahydrocurcuminoids. Biochem Pharmacol 52: 519-525.
[18] Bylka W, Znajdek-Awiżeń P, Studzińska-Sroka E, Brzezińska M (2013) Centella asiatica in cosmetology. Postepy Dermatol Alergol 30: 46-49.
[19] Rubik B, Muehsam D, Hammerschlag R, Jain S (2015) Biofield science and healing: history, terminology, and concepts. Glob Adv Health Med 4: 8-14.
[20] Barnes PM, Bloom B, Nahin RL (2008) Complementary and alternative medicine use among adults and children: United States, 2007. Natl Health Stat Report 12: 1-23.
[21] Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, Mondal SC, Jana S (2015) Antibiogram pattern of Shigella flexneri: Effect of biofield treatment. Air Water Borne Diseases 3: 122.
[22] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) Antimicrobial susceptibility pattern and biochemical characteristics of Staphylococcus aureus: Impact of bio field treatment. J Microb Biochem Technol 7: 238-241.
[23] Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, Mondal SC, Jana S (2015) Effect of biofield energy treatment on Streptococcus group B: A postpartum pathogen. J Microb Biochem Technol 7: 269-273.
[24] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4: 141.
[25] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In Vitro evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther 7: 253-257.
[26] Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Morphological and molecular analysis using RAPD in biofield treated sponge and bitter gourd. American Journal of Agriculture and Forestry 3: 264-270.
[27] Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Effect of biofield energy treatment on chlorophyll content, pathological study, and molecular analysis of cashew plant (Anacardium occidentale L.). Journal of Plant Sciences 3: 372-382.
[28] Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2016) Molecular analysis of biofield treated eggplant and watermelon crops. Adv Crop Sci Tech 4: 208.
[29] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Spectroscopic characterization of chloramphenicol and tetracycline: An impact of biofield. Pharm Anal Acta 6:395.
[30] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Spectroscopic characterization of biofield treated metronidazole and tinidazole. Med Chem 5: 340-344.
[31] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Effect of biofield treatment on spectral properties of paracetamol and piroxicam. Chem Sci J 6: 98.
[32] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Jana S, Mishra R (2015) Bio-field treatment: An effective strategy to improve the quality of beef extract and meat infusion powder. J Nutr Food Sci 5: 389.
[33] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) An evaluation of biofield treatment on thermal, physical and structural properties of cadmium powder. J Thermodyn Catal 6: 147.
[34] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) Effect of Biofield energy treatment on physical and structural properties of calcium carbide and praseodymium oxide. International Journal of Materials Science and Applications 4: 390-395.
[35] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Characterization of physical, thermal and structural properties of chromium (VI) oxide powder: impact of biofield treatment. J Powder Metall Min 4: 128.
[36] Plumb JA (2004) Cell sensitivity assays: The MTT assay. Methods Mol Med 88: 165-169.
[37] Hahn MS, Kobler JB, Starcher BC, Zeitels SM, Langer R (2006) Quantitative and comparative studies of the vocal fold extracellular matrix. I: Elastic fibers and hyaluronic acid. Ann Otol Rhinol Laryngol 115: 156-164.
[38] Zhang L, Yoshida T, Kuroiwa Y (1992) Stimulation of melanin synthesis of B16-F10 mouse melanoma cells by bufalin. Life Sci 51: 17-24.
[39] Shoulders MD, Raines RT (2009) Collagen structure and stability. Annual review of biochemistry 78: 929-958.
[40] Fronza M, Heinzmann B, Hamburger M, Laufer S, Merfort I (2009) Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol 126: 463-467.
[41] Kadler KE, Holmes DF, Trotter JA, Chapman JA (1996) Collagen fibril formation. Biochem J 316: 1-11.
[42] Weindl G, Schaller M, Schäfer-Korting M, Korting HC (2004) Hyaluronic acid in the treatment and prevention of skin diseases: molecular biological, pharmaceutical and clinical aspects. Skin Pharmacol Physiol 17: 207-213.
[43] Busca R, Ballotti R (2000) Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigment Cell Res 13: 60-69.
[44] Bylka W, Znajdek-Awiżeń P, Studzińska-Sroka E, Brzezińska M (2013) Centella asiatica in cosmetology. Postepy Dermatol Alergol 30: 46-49.
[45] Prasad S, Tyagi AK, Aggarwal BB (2014) Recent developments in delivery, bioavailability, absorption and metabolism of curcumin: the golden pigment from golden spice. Cancer Res Treat 46: 2-18.
[46] Alaluf S, Atkins D, Barrett K, Blount M, Carter N, Heath A (2002) The impact of epidermal melanin on objective measurements of human skin colour. Pigment Cell Res 15: 119-126.
[47] Ho JN, Lee YH, Lee YD, Jun WJ, Kim HK, Hong BS, Shin DH, Cho HY (2005) Inhibitory effect of Aucubin isolated from Eucommia ulmoides against UVB induced matrix metalloproteinase-1 production in human skin fibroblasts. Biosci Biotechnol Biochem 69: 2227-2231.
[48] Liang CC, Park AY, Guan JL (2007) In vitro scratch assay: A convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2: 329-333.
Cite This Article
  • APA Style

    Eileen Mary Meagher, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. (2017). An in Vitro Study of Biofield Energy Healing Based Herbomineral Formulation for Skin Protection. American Journal of Laboratory Medicine, 2(2), 13-23. https://doi.org/10.11648/j.ajlm.20170202.11

    Copy | Download

    ACS Style

    Eileen Mary Meagher; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak, et al. An in Vitro Study of Biofield Energy Healing Based Herbomineral Formulation for Skin Protection. Am. J. Lab. Med. 2017, 2(2), 13-23. doi: 10.11648/j.ajlm.20170202.11

    Copy | Download

    AMA Style

    Eileen Mary Meagher, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. An in Vitro Study of Biofield Energy Healing Based Herbomineral Formulation for Skin Protection. Am J Lab Med. 2017;2(2):13-23. doi: 10.11648/j.ajlm.20170202.11

    Copy | Download

  • @article{10.11648/j.ajlm.20170202.11,
      author = {Eileen Mary Meagher and Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Mayank Gangwar and Snehasis Jana},
      title = {An in Vitro Study of Biofield Energy Healing Based Herbomineral Formulation for Skin Protection},
      journal = {American Journal of Laboratory Medicine},
      volume = {2},
      number = {2},
      pages = {13-23},
      doi = {10.11648/j.ajlm.20170202.11},
      url = {https://doi.org/10.11648/j.ajlm.20170202.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20170202.11},
      abstract = {The current research work deals with the impact of the Consciousness Energy Healing Treatment (The Trivedi Effect®) based herbomineral test formulation and cell medium (DMEM) against various skin health parameters. The test formulation is the combination of minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts, i.e. one part received the Biofield Energy Treatment by Eileen Mary Meagher and was labeled as the Biofield Treated (BT) samples, while other represented was defined as the untreated test (UT) samples. The MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability. Cell proliferation assay using BrdU method showed an improved proliferation rate by 343.23%, 316.13%, and 512.75% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 µg/mL, compared with the untreated group. The collagen level was significantly increased by 15.70% and 13.52% at 2.5 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The level of elastin was increased by 47.0% and 64.1% at 2.5 and 1.25 µg/mL, respectively in the BT-DMEM + UT-Test formulation, group compared with the untreated group. Hyaluronic acid synthesis was increased by 4.37%, 41.47%, and 19.74% at 2.5, 1.25, and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation group with respect to the untreated group. Melanin synthesis was significantly inhibited by 8.83%, 5.78%, and 12.68% at 0.0125, 0.0625, and 0.125 µg/mL, respectively in the UT-DMEM + BT-Test formulation group compared with the untreated group. Anti-wrinkling effect showed an improved cell viability by 4.32% and 2.47% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT- Test formulation groups, respectively in HFF-1 cells compared with the untreated group. Wound healing activity using scratch assay exhibited improved a healing rate up to 6% in the HFF-1 and HaCaT cells lines in the Biofield Energy Treated Test formulation. In conclusion, results suggest that The Trivedi Effect®- Consciousness Energy Healing Treatment based test formulation and medium have the ability to improve the skin health and can be used as skincare products cosmetics for anti-ageing, anti-wrinkling action and for the treatment of many skin diseases such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, etc.},
     year = {2017}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - An in Vitro Study of Biofield Energy Healing Based Herbomineral Formulation for Skin Protection
    AU  - Eileen Mary Meagher
    AU  - Mahendra Kumar Trivedi
    AU  - Alice Branton
    AU  - Dahryn Trivedi
    AU  - Gopal Nayak
    AU  - Mayank Gangwar
    AU  - Snehasis Jana
    Y1  - 2017/05/02
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajlm.20170202.11
    DO  - 10.11648/j.ajlm.20170202.11
    T2  - American Journal of Laboratory Medicine
    JF  - American Journal of Laboratory Medicine
    JO  - American Journal of Laboratory Medicine
    SP  - 13
    EP  - 23
    PB  - Science Publishing Group
    SN  - 2575-386X
    UR  - https://doi.org/10.11648/j.ajlm.20170202.11
    AB  - The current research work deals with the impact of the Consciousness Energy Healing Treatment (The Trivedi Effect®) based herbomineral test formulation and cell medium (DMEM) against various skin health parameters. The test formulation is the combination of minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts, i.e. one part received the Biofield Energy Treatment by Eileen Mary Meagher and was labeled as the Biofield Treated (BT) samples, while other represented was defined as the untreated test (UT) samples. The MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability. Cell proliferation assay using BrdU method showed an improved proliferation rate by 343.23%, 316.13%, and 512.75% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 µg/mL, compared with the untreated group. The collagen level was significantly increased by 15.70% and 13.52% at 2.5 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The level of elastin was increased by 47.0% and 64.1% at 2.5 and 1.25 µg/mL, respectively in the BT-DMEM + UT-Test formulation, group compared with the untreated group. Hyaluronic acid synthesis was increased by 4.37%, 41.47%, and 19.74% at 2.5, 1.25, and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation group with respect to the untreated group. Melanin synthesis was significantly inhibited by 8.83%, 5.78%, and 12.68% at 0.0125, 0.0625, and 0.125 µg/mL, respectively in the UT-DMEM + BT-Test formulation group compared with the untreated group. Anti-wrinkling effect showed an improved cell viability by 4.32% and 2.47% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT- Test formulation groups, respectively in HFF-1 cells compared with the untreated group. Wound healing activity using scratch assay exhibited improved a healing rate up to 6% in the HFF-1 and HaCaT cells lines in the Biofield Energy Treated Test formulation. In conclusion, results suggest that The Trivedi Effect®- Consciousness Energy Healing Treatment based test formulation and medium have the ability to improve the skin health and can be used as skincare products cosmetics for anti-ageing, anti-wrinkling action and for the treatment of many skin diseases such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, etc.
    VL  - 2
    IS  - 2
    ER  - 

    Copy | Download

Author Information
  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Sections