International Journal of Ophthalmology & Visual Science

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Involvement of ER Stress in Human Primary Pterygium

Received: 13 June 2019    Accepted: 02 July 2019    Published: 11 July 2019
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Abstract

Purpose: The aim of this study was to investigate the ER stress activation in human primary pterygium. Methods and materials: Human primary pterygium or normal Tenon's capsule tissues were obtained from patients with primary pterygium following surgical excision or from normal human fresh cadaver eyes. The tissues were processed within 2 hours. The mRNA or protein specimens were extracted from those tissues for analysis, cryosections of those tissues were prepared for immunohistochemical staining. The mRNA levels of endoplasmic reticulum (ER) stress-related factors in those tissues were detected by qPCR analysis and the related proteins levels were detected by qPCR analysis and immunohistochemical staining or western blotting. Results: The ER stress-related gene transcription levels of GRP78,spliced XBP-1, ATF4 and ATF6 and the protein expression levels of GRP78, p-IRE1α, p-eIF2α and ATF6 were all increased in the human primary pterygium tissues when compared with the normal control tissues. Conclusion: The results in this study suggest that the three unfolded protein response pathways are all activated in the human primary pterygium tissues, which indicates that the ER stress is involved in the progression of pterygium, and also suggests a potential mechanism of ER stress-induced inflammation in the human primary pterygium tissues.

DOI 10.11648/j.ijovs.20190402.11
Published in International Journal of Ophthalmology & Visual Science (Volume 4, Issue 2, June 2019)
Page(s) 30-36
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Primary Pterygium, Endoplasmic Reticulum Stress, Unfolded Protein Response, Inflammation, UPR Pathway Activation

References
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Author Information
  • State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China

  • State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China

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  • APA Style

    Sheng Zhou, Jing Yang. (2019). Involvement of ER Stress in Human Primary Pterygium. International Journal of Ophthalmology & Visual Science, 4(2), 30-36. https://doi.org/10.11648/j.ijovs.20190402.11

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    ACS Style

    Sheng Zhou; Jing Yang. Involvement of ER Stress in Human Primary Pterygium. Int. J. Ophthalmol. Vis. Sci. 2019, 4(2), 30-36. doi: 10.11648/j.ijovs.20190402.11

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    AMA Style

    Sheng Zhou, Jing Yang. Involvement of ER Stress in Human Primary Pterygium. Int J Ophthalmol Vis Sci. 2019;4(2):30-36. doi: 10.11648/j.ijovs.20190402.11

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  • @article{10.11648/j.ijovs.20190402.11,
      author = {Sheng Zhou and Jing Yang},
      title = {Involvement of ER Stress in Human Primary Pterygium},
      journal = {International Journal of Ophthalmology & Visual Science},
      volume = {4},
      number = {2},
      pages = {30-36},
      doi = {10.11648/j.ijovs.20190402.11},
      url = {https://doi.org/10.11648/j.ijovs.20190402.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijovs.20190402.11},
      abstract = {Purpose: The aim of this study was to investigate the ER stress activation in human primary pterygium. Methods and materials: Human primary pterygium or normal Tenon's capsule tissues were obtained from patients with primary pterygium following surgical excision or from normal human fresh cadaver eyes. The tissues were processed within 2 hours. The mRNA or protein specimens were extracted from those tissues for analysis, cryosections of those tissues were prepared for immunohistochemical staining. The mRNA levels of endoplasmic reticulum (ER) stress-related factors in those tissues were detected by qPCR analysis and the related proteins levels were detected by qPCR analysis and immunohistochemical staining or western blotting. Results: The ER stress-related gene transcription levels of GRP78,spliced XBP-1, ATF4 and ATF6 and the protein expression levels of GRP78, p-IRE1α, p-eIF2α and ATF6 were all increased in the human primary pterygium tissues when compared with the normal control tissues. Conclusion: The results in this study suggest that the three unfolded protein response pathways are all activated in the human primary pterygium tissues, which indicates that the ER stress is involved in the progression of pterygium, and also suggests a potential mechanism of ER stress-induced inflammation in the human primary pterygium tissues.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Involvement of ER Stress in Human Primary Pterygium
    AU  - Sheng Zhou
    AU  - Jing Yang
    Y1  - 2019/07/11
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ijovs.20190402.11
    DO  - 10.11648/j.ijovs.20190402.11
    T2  - International Journal of Ophthalmology & Visual Science
    JF  - International Journal of Ophthalmology & Visual Science
    JO  - International Journal of Ophthalmology & Visual Science
    SP  - 30
    EP  - 36
    PB  - Science Publishing Group
    SN  - 2637-3858
    UR  - https://doi.org/10.11648/j.ijovs.20190402.11
    AB  - Purpose: The aim of this study was to investigate the ER stress activation in human primary pterygium. Methods and materials: Human primary pterygium or normal Tenon's capsule tissues were obtained from patients with primary pterygium following surgical excision or from normal human fresh cadaver eyes. The tissues were processed within 2 hours. The mRNA or protein specimens were extracted from those tissues for analysis, cryosections of those tissues were prepared for immunohistochemical staining. The mRNA levels of endoplasmic reticulum (ER) stress-related factors in those tissues were detected by qPCR analysis and the related proteins levels were detected by qPCR analysis and immunohistochemical staining or western blotting. Results: The ER stress-related gene transcription levels of GRP78,spliced XBP-1, ATF4 and ATF6 and the protein expression levels of GRP78, p-IRE1α, p-eIF2α and ATF6 were all increased in the human primary pterygium tissues when compared with the normal control tissues. Conclusion: The results in this study suggest that the three unfolded protein response pathways are all activated in the human primary pterygium tissues, which indicates that the ER stress is involved in the progression of pterygium, and also suggests a potential mechanism of ER stress-induced inflammation in the human primary pterygium tissues.
    VL  - 4
    IS  - 2
    ER  - 

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