Maximizing Stability in Industrial Enzymes: Rational Design Approach – A Review

Abubakar Muhammad Nazif; Ayuba Yohanna Musa; Muhammad Muawiya Alkali; Ilesanmi Esther

doi:doi:10.11648/j.ajche.20170506.13

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Maximizing Stability in Industrial Enzymes: Rational Design Approach – A Review

Abubakar Muhammad Nazif, Ayuba Yohanna Musa, Muhammad Muawiya Alkali, Ilesanmi Esther

Received: 13 September 2017 Accepted: 26 September 2017 Published: 13 November 2017

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Abstract

Indigenous enzymes found in nature have found wide application in industries ascribable to their ability to catalyze complex chemical processes under moderate experimental and environmental conditions. However, the use of indigenous enzymes is yet to achieve the needed industrial goal for, indigenous enzymes are readily unstable when subjected to harsh environmental conditions. Since the emergence of recombinant DNA technology and recent developments in protein engineering in recent years, there have been continuous reports regarding enzyme stability – most especially by the introduction of site-directed mutagenesis. With these new developments, scientists have been able to engineer enzymes using a variety of strategies in rational design such as the introduction of disulfide bridges and engineering hydrophobic residues. This review aims to highlight rational design methods and enzyme immobilization from various studies, which may be used to increase stability in industrial enzymes.

DOI	10.11648/j.ajche.20170506.13
Published in	American Journal of Chemical Engineering (Volume 5, Issue 6, November 2017)
Page(s)	135-139
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Protein Engineering, Stability, Rational Design, Immobilization

References

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APA Style

Abubakar Muhammad Nazif, Ayuba Yohanna Musa, Muhammad Muawiya Alkali, Ilesanmi Esther. (2017). Maximizing Stability in Industrial Enzymes: Rational Design Approach – A Review. American Journal of Chemical Engineering, 5(6), 135-139. https://doi.org/10.11648/j.ajche.20170506.13

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ACS Style

Abubakar Muhammad Nazif; Ayuba Yohanna Musa; Muhammad Muawiya Alkali; Ilesanmi Esther. Maximizing Stability in Industrial Enzymes: Rational Design Approach – A Review. Am. J. Chem. Eng. 2017, 5(6), 135-139. doi: 10.11648/j.ajche.20170506.13

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AMA Style

Abubakar Muhammad Nazif, Ayuba Yohanna Musa, Muhammad Muawiya Alkali, Ilesanmi Esther. Maximizing Stability in Industrial Enzymes: Rational Design Approach – A Review. Am J Chem Eng. 2017;5(6):135-139. doi: 10.11648/j.ajche.20170506.13

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@article{10.11648/j.ajche.20170506.13,
  author = {Abubakar Muhammad Nazif and Ayuba Yohanna Musa and Muhammad Muawiya Alkali and Ilesanmi Esther},
  title = {Maximizing Stability in Industrial Enzymes: Rational Design Approach – A Review},
  journal = {American Journal of Chemical Engineering},
  volume = {5},
  number = {6},
  pages = {135-139},
  doi = {10.11648/j.ajche.20170506.13},
  url = {https://doi.org/10.11648/j.ajche.20170506.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajche.20170506.13},
  abstract = {Indigenous enzymes found in nature have found wide application in industries ascribable to their ability to catalyze complex chemical processes under moderate experimental and environmental conditions. However, the use of indigenous enzymes is yet to achieve the needed industrial goal for, indigenous enzymes are readily unstable when subjected to harsh environmental conditions. Since the emergence of recombinant DNA technology and recent developments in protein engineering in recent years, there have been continuous reports regarding enzyme stability – most especially by the introduction of site-directed mutagenesis. With these new developments, scientists have been able to engineer enzymes using a variety of strategies in rational design such as the introduction of disulfide bridges and engineering hydrophobic residues. This review aims to highlight rational design methods and enzyme immobilization from various studies, which may be used to increase stability in industrial enzymes.},
 year = {2017}
}

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TY  - JOUR
T1  - Maximizing Stability in Industrial Enzymes: Rational Design Approach – A Review
AU  - Abubakar Muhammad Nazif
AU  - Ayuba Yohanna Musa
AU  - Muhammad Muawiya Alkali
AU  - Ilesanmi Esther
Y1  - 2017/11/13
PY  - 2017
N1  - https://doi.org/10.11648/j.ajche.20170506.13
DO  - 10.11648/j.ajche.20170506.13
T2  - American Journal of Chemical Engineering
JF  - American Journal of Chemical Engineering
JO  - American Journal of Chemical Engineering
SP  - 135
EP  - 139
PB  - Science Publishing Group
SN  - 2330-8613
UR  - https://doi.org/10.11648/j.ajche.20170506.13
AB  - Indigenous enzymes found in nature have found wide application in industries ascribable to their ability to catalyze complex chemical processes under moderate experimental and environmental conditions. However, the use of indigenous enzymes is yet to achieve the needed industrial goal for, indigenous enzymes are readily unstable when subjected to harsh environmental conditions. Since the emergence of recombinant DNA technology and recent developments in protein engineering in recent years, there have been continuous reports regarding enzyme stability – most especially by the introduction of site-directed mutagenesis. With these new developments, scientists have been able to engineer enzymes using a variety of strategies in rational design such as the introduction of disulfide bridges and engineering hydrophobic residues. This review aims to highlight rational design methods and enzyme immobilization from various studies, which may be used to increase stability in industrial enzymes.
VL  - 5
IS  - 6
ER  -

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Author Information

Abubakar Muhammad Nazif

Department of Biotechnology, Modibbo Adama University of Technology, Yola, Nigeria
Ayuba Yohanna Musa

Department of Chemistry, Abubakar Tafawa Balewa University, Bauchi, Nigeria
Muhammad Muawiya Alkali

Department of Biochemistry, Modibbo Adama University of Technology, Yola, Nigeria
Ilesanmi Esther

Department of Chemistry, University of Benin, Benin, Nigeria

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APA Style

Abubakar Muhammad Nazif, Ayuba Yohanna Musa, Muhammad Muawiya Alkali, Ilesanmi Esther. (2017). Maximizing Stability in Industrial Enzymes: Rational Design Approach – A Review. American Journal of Chemical Engineering, 5(6), 135-139. https://doi.org/10.11648/j.ajche.20170506.13

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ACS Style

Abubakar Muhammad Nazif; Ayuba Yohanna Musa; Muhammad Muawiya Alkali; Ilesanmi Esther. Maximizing Stability in Industrial Enzymes: Rational Design Approach – A Review. Am. J. Chem. Eng. 2017, 5(6), 135-139. doi: 10.11648/j.ajche.20170506.13

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AMA Style

Abubakar Muhammad Nazif, Ayuba Yohanna Musa, Muhammad Muawiya Alkali, Ilesanmi Esther. Maximizing Stability in Industrial Enzymes: Rational Design Approach – A Review. Am J Chem Eng. 2017;5(6):135-139. doi: 10.11648/j.ajche.20170506.13

Copy | Download

@article{10.11648/j.ajche.20170506.13,
  author = {Abubakar Muhammad Nazif and Ayuba Yohanna Musa and Muhammad Muawiya Alkali and Ilesanmi Esther},
  title = {Maximizing Stability in Industrial Enzymes: Rational Design Approach – A Review},
  journal = {American Journal of Chemical Engineering},
  volume = {5},
  number = {6},
  pages = {135-139},
  doi = {10.11648/j.ajche.20170506.13},
  url = {https://doi.org/10.11648/j.ajche.20170506.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajche.20170506.13},
  abstract = {Indigenous enzymes found in nature have found wide application in industries ascribable to their ability to catalyze complex chemical processes under moderate experimental and environmental conditions. However, the use of indigenous enzymes is yet to achieve the needed industrial goal for, indigenous enzymes are readily unstable when subjected to harsh environmental conditions. Since the emergence of recombinant DNA technology and recent developments in protein engineering in recent years, there have been continuous reports regarding enzyme stability – most especially by the introduction of site-directed mutagenesis. With these new developments, scientists have been able to engineer enzymes using a variety of strategies in rational design such as the introduction of disulfide bridges and engineering hydrophobic residues. This review aims to highlight rational design methods and enzyme immobilization from various studies, which may be used to increase stability in industrial enzymes.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Maximizing Stability in Industrial Enzymes: Rational Design Approach – A Review
AU  - Abubakar Muhammad Nazif
AU  - Ayuba Yohanna Musa
AU  - Muhammad Muawiya Alkali
AU  - Ilesanmi Esther
Y1  - 2017/11/13
PY  - 2017
N1  - https://doi.org/10.11648/j.ajche.20170506.13
DO  - 10.11648/j.ajche.20170506.13
T2  - American Journal of Chemical Engineering
JF  - American Journal of Chemical Engineering
JO  - American Journal of Chemical Engineering
SP  - 135
EP  - 139
PB  - Science Publishing Group
SN  - 2330-8613
UR  - https://doi.org/10.11648/j.ajche.20170506.13
AB  - Indigenous enzymes found in nature have found wide application in industries ascribable to their ability to catalyze complex chemical processes under moderate experimental and environmental conditions. However, the use of indigenous enzymes is yet to achieve the needed industrial goal for, indigenous enzymes are readily unstable when subjected to harsh environmental conditions. Since the emergence of recombinant DNA technology and recent developments in protein engineering in recent years, there have been continuous reports regarding enzyme stability – most especially by the introduction of site-directed mutagenesis. With these new developments, scientists have been able to engineer enzymes using a variety of strategies in rational design such as the introduction of disulfide bridges and engineering hydrophobic residues. This review aims to highlight rational design methods and enzyme immobilization from various studies, which may be used to increase stability in industrial enzymes.
VL  - 5
IS  - 6
ER  -

Copy | Download