Omeprazole is a potent proton pump inhibitor for the treatment of various acid-related gastrointestinal disorders. simple, selective and rapid reversed phase high performance liquid chromatographic (RP-HPLC) method for the analysis of Omeprazole has been developed and validated. The separation was achieved from HPLC column (ZORBAX, XDB, C-18 (150×4.6 mm, 5 micron) with a mobile phase consisting of HPLC grade acetonitrile and phosphate buffer solution (pH 7.4) in the ratio of (60:40 v/v) at a flow rate of 0.5 ml/min and runtime 10 min with UV detector at 302nm. The method was specific and it was observed that no interference with diluents. The proposed method was accurate with 100.95% recovery omeprazole and precise (% RSD of area of system precision, % RSD of assay of method precision were found to be 0.14% and 0.37% respectively). From the linearity study the correlation coefficient is found to be 0.9988, which indicated that the method was linear over 25% to 175% range. The method was found robust for possible changes. Therefore, this method can be used as a more convenient and efficient option for the analysis of Omeprazole to establish the quality of the drug substance during routine analysis with consistent and reproducible results.
| Published in | American Journal of Chemical Engineering (Volume 6, Issue 1) |
| DOI | 10.11648/j.ajche.20180601.11 |
| Page(s) | 1-6 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Omeprazole, HPLC, Validation, Mobile Phase
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APA Style
Mahmoud Mohamed Ali, Eshtiag Abdalla Ibrahim, Mohamed N. Abdalaziz. (2018). Development and Validation for RP-HPLC Method of Assay of Omeprazole Capsules Formulation. American Journal of Chemical Engineering, 6(1), 1-6. https://doi.org/10.11648/j.ajche.20180601.11
ACS Style
Mahmoud Mohamed Ali; Eshtiag Abdalla Ibrahim; Mohamed N. Abdalaziz. Development and Validation for RP-HPLC Method of Assay of Omeprazole Capsules Formulation. Am. J. Chem. Eng. 2018, 6(1), 1-6. doi: 10.11648/j.ajche.20180601.11
AMA Style
Mahmoud Mohamed Ali, Eshtiag Abdalla Ibrahim, Mohamed N. Abdalaziz. Development and Validation for RP-HPLC Method of Assay of Omeprazole Capsules Formulation. Am J Chem Eng. 2018;6(1):1-6. doi: 10.11648/j.ajche.20180601.11
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Download@article{10.11648/j.ajche.20180601.11,
author = {Mahmoud Mohamed Ali and Eshtiag Abdalla Ibrahim and Mohamed N. Abdalaziz},
title = {Development and Validation for RP-HPLC Method of Assay of Omeprazole Capsules Formulation},
journal = {American Journal of Chemical Engineering},
volume = {6},
number = {1},
pages = {1-6},
doi = {10.11648/j.ajche.20180601.11},
url = {https://doi.org/10.11648/j.ajche.20180601.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajche.20180601.11},
abstract = {Omeprazole is a potent proton pump inhibitor for the treatment of various acid-related gastrointestinal disorders. simple, selective and rapid reversed phase high performance liquid chromatographic (RP-HPLC) method for the analysis of Omeprazole has been developed and validated. The separation was achieved from HPLC column (ZORBAX, XDB, C-18 (150×4.6 mm, 5 micron) with a mobile phase consisting of HPLC grade acetonitrile and phosphate buffer solution (pH 7.4) in the ratio of (60:40 v/v) at a flow rate of 0.5 ml/min and runtime 10 min with UV detector at 302nm. The method was specific and it was observed that no interference with diluents. The proposed method was accurate with 100.95% recovery omeprazole and precise (% RSD of area of system precision, % RSD of assay of method precision were found to be 0.14% and 0.37% respectively). From the linearity study the correlation coefficient is found to be 0.9988, which indicated that the method was linear over 25% to 175% range. The method was found robust for possible changes. Therefore, this method can be used as a more convenient and efficient option for the analysis of Omeprazole to establish the quality of the drug substance during routine analysis with consistent and reproducible results.},
year = {2018}
}
TY - JOUR T1 - Development and Validation for RP-HPLC Method of Assay of Omeprazole Capsules Formulation AU - Mahmoud Mohamed Ali AU - Eshtiag Abdalla Ibrahim AU - Mohamed N. Abdalaziz Y1 - 2018/01/09 PY - 2018 N1 - https://doi.org/10.11648/j.ajche.20180601.11 DO - 10.11648/j.ajche.20180601.11 T2 - American Journal of Chemical Engineering JF - American Journal of Chemical Engineering JO - American Journal of Chemical Engineering SP - 1 EP - 6 PB - Science Publishing Group SN - 2330-8613 UR - https://doi.org/10.11648/j.ajche.20180601.11 AB - Omeprazole is a potent proton pump inhibitor for the treatment of various acid-related gastrointestinal disorders. simple, selective and rapid reversed phase high performance liquid chromatographic (RP-HPLC) method for the analysis of Omeprazole has been developed and validated. The separation was achieved from HPLC column (ZORBAX, XDB, C-18 (150×4.6 mm, 5 micron) with a mobile phase consisting of HPLC grade acetonitrile and phosphate buffer solution (pH 7.4) in the ratio of (60:40 v/v) at a flow rate of 0.5 ml/min and runtime 10 min with UV detector at 302nm. The method was specific and it was observed that no interference with diluents. The proposed method was accurate with 100.95% recovery omeprazole and precise (% RSD of area of system precision, % RSD of assay of method precision were found to be 0.14% and 0.37% respectively). From the linearity study the correlation coefficient is found to be 0.9988, which indicated that the method was linear over 25% to 175% range. The method was found robust for possible changes. Therefore, this method can be used as a more convenient and efficient option for the analysis of Omeprazole to establish the quality of the drug substance during routine analysis with consistent and reproducible results. VL - 6 IS - 1 ER -
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