The Valuation of Genetic Diversity in Tarchonanthus camphoratus Plant Using ISSR Markers in Taif, KSA
American Journal of Agriculture and Forestry
Volume 7, Issue 6, November 2019, Pages: 277-281
Received: Sep. 1, 2019; Accepted: Oct. 5, 2019; Published: Nov. 8, 2019
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Authors
Hatim Matouq Alyasi, Biological Science, Taif Unoversity, Taif, Saudi Arabia
Rahmah Nasser Alqthanin, Biological Science, King Khalid University, Abha, Saudi Arabia
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Abstract
Genetic variation among T. camphoratus individual plant samples collected from Taif region, Saudi Arabia was assessed using inter-simple sequence repeat (ISSR) markers. Eleven ISSR primers were used to evaluate T. camphoratus samples. The number of polymorphic bands per primer varied from 5 to 16, with 10.3 bands per primer on average. Nei’s genetic distance between T. camphoratus samples ranged from 0.03 to 0.94. According to genetic similarity and intraspecific differentiation, the 15 T. camphoratus were grouped into two main different clusters with about 0.67 genetic similarity. It was not possible to classify the samples according to their geographic origin, showing that there is no structure in the gene bank. Cluster analysis is indicating good resolution of genetic diversity in Tarchonanthus germplasm using ISSR markers. Our results indicate that ISSR can be useful for genetic diversity studies, to provide practical information for parental selection, biodiversity and to assist breeding and conservation strategies.
Keywords
Tarchonanthus camphoratus, Genetic Variability, Molecular Markers, Genetic Resources
To cite this article
Hatim Matouq Alyasi, Rahmah Nasser Alqthanin, The Valuation of Genetic Diversity in Tarchonanthus camphoratus Plant Using ISSR Markers in Taif, KSA, American Journal of Agriculture and Forestry. Vol. 7, No. 6, 2019, pp. 277-281. doi: 10.11648/j.ajaf.20190706.15
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Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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