American Journal of Agriculture and Forestry

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Effect of Biofertilizers on the Phenolic Content in a Hybrid Family of Cacao After Leaf Infection with Phytophthora megakarya and Exogenous Application of Salicylic Acid

Received: 31 March 2019    Accepted: 07 May 2019    Published: 29 May 2019
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Abstract

In order to protect cacao against Phytophthora megakarya, the most aggressive pathogen of this plant in Cameroon, a study was carried out on hybrid genotypes of the family F79SA of cacao (Theobroma cacao L.) to investigate the effect of inoculation of the biofertilizers Gigaspora margarita and Acaulospora tuberculata on the phenolic compound content in hybrid genotypes after leaf infection with Phytophthora megakarya and treatment of salicylic acid (SA). Thus, the phenolic compound content of hybrid genotypes of the family F79SA of T. cacao was evaluated after artificial infection of leaves with P. megakarya and treatment of salicylic acid without control and under control of biofertilizers. The artificial infection of P. megakarya and exogenous application of salicylic acid resulted in an increase in the accumulation of phenolic compounds (PC) in all genotypes. This increase was more important under the control of Gigaspora margarita and Acaulospora tuberculata and varied from one genotype to another. The PC content analysis map of these genotypes at different treatment conditions under the control of biofertilizers showed a gradual evolution of black coloration, a sign of the increase in phenolic compound content related to concentrations of salicylic acid and infected leaves in all hybrid genotypes thus expressing high tolerance. This map allowed to classify hybrid genotypes according to their level of tolerance. A negative and significant correlation (P = 0.05) was observed between the development of necrosis and the accumulation of phenolic compounds on one hand and between salicylic acid and the accumulation of phenolic compounds on the other hand. Salicylic acid can therefore be used in the cacao selection program in the absence of the pathogen for the identification of hybrid cacao genotypes as well as in other similar breeding programs.

DOI 10.11648/j.ajaf.20190703.11
Published in American Journal of Agriculture and Forestry (Volume 7, Issue 3, May 2019)
Page(s) 84-94
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Theobroma cacao, Phytophthora megakarya, Gigaspora margarita, Acaulospora tuberculata, Tolerance, Salicylic Acid, Phenolic Compound

References
[1] Fidelis C, Rajashekhar Rao B K. Enriched Cocoa pod composts and their effects on hybrid Cocoa seedlings. International Journal of Recycling of Organic Waste in Agriculture. 2017, 6 (2): 99–106.
[2] Shahin S A, Jonathan S, Lary D J, et al. Phytophthora megakarya and Phytophthora palmivora, Closely Related Causal Agents of Cacao Black Pod Rot, underwent increases in genome sizes and gene numbers by different mechanisms. Genome Biology and Evolution. 2017a, 9 (3): 536–557.
[3] Shahin S A, Jonathan S, Lary D J, et al. Phytophthora megakarya and P. palmivora, causal agents of black pod rot, induce similar plant defense responses late during infection of susceptible cacao pods. Frontiers in Plant Science. 2017b, 8: 1-18.
[4] Gidoin C, Babin R, Bagny Beilhe L, et al. Tree Spatial Structure, Host Composition and Resource Availability Influence Mirid Density or Black Pod Prevalence in Cacao Agroforests in Cameroon. PLoS ONE. 2014, 9: e109405. doi: 10.1371/journal.pone.0109405.
[5] Doungous O, Minyaka E, Longue E, et al. Potentials of Cocoa pod husk-based compost on Phytophthora pod rot disease suppression, soil fertility, and Theobroma cacao L. growth. Environmental Science and Pollution Research. 2018, 25: 25327–25335.
[6] Ndoumbe-Nkeng M, Efombagn M I B, Bidzanga N L, et al. Spatio-temporal dynamics on a plot scale of Cocoa black pod rot caused by Phytophthora megakarya in Cameroon. European Journal of Plant Pathology. 2017, 147: 579–590.
[7] Simo C, Djocgoue P F, Minyaka E, et al. Guaiacol Peroxidase heritability in tolerance of Cocoa (Theobroma cacao L.) to Phytophthora megakarya, agent of Cocoa black pod disease. International Journal of Agricultural Policy and Research. 2018, 6 (2): 7-20.
[8] Deberdt P, Mfegue C V, Tondje P R, et al. Impact of environmental factors, chemical fungicide and biological control on cacao pod production dynamics and black pod disease (Phytophthora megakarya) in Cameroon. Biological Control. 2008, 44 (2): 149-159.
[9] Opoku I, Akrofi A, Appiah A. Assessment of sanitation and fungicide application directed at Cocoa tree trunks for the control of Phytophthora black pod infections in pods growing in the canopy. European Journal of Plant Pathology. 2007, 117: 167-175.
[10] Nyadanu D, Akromah R, Adomako B, et al. Host plant resistance to Phytophthora pod rot in Cocoa (Theobroma cacao L.): The role of epicuticular wax on pod and leaf surfaces. International Journal of botany. 2012, 8 (1): 13-21.
[11] Akthar M S, Siddiqui Z A. Arbuscular Mycorrhizal Fungi as Potential Bioprotectants against Plant Pathogens. Sustainable Agriculture and Forestry. 2008, 61-97.
[12] Joner E J, Leyval C. Rhizosphere gradients of polycyclic aramatic hydrocarbon (PAH) dissispation in two industrial soils and the impact of arbuscular mycorrhiza. Environmental Science and Technology. 2003, 37 (11): 2371-2375.
[13] Fokom R, Nana W L, Tchameni S, et al. Arbuscular Mycorrhizal Fungi (AMF) colonisation and rhizobia nodulation of cowpeaas affected by flavonoid application. Research Journal of Agriculture and Biological Sciences. 2010, 6 (6): 1015-1021.
[14] Simo C, Djocgoue P F, Mbouobda H D, et al. Assessing relationship between phenolic compounds and resistance to Phytophthora megakarya using two Cocoa (Theobroma cacao L.) families. African Journal of Biotechnology. 2014, 13: 2956-2965.
[15] Hayat Q, Hayat S, Irfan M, et al. Effect of exogenous Salicylic sacid under changing environment. Environmental and Experimental Botany. 2010, 68: 14-25.
[16] Bechtold U, Karpinski S, Mullineaux P. The influence of the light environment and photosynthesis on oxidative signalling responses in plant-biotrophic pathogen interactions. Plant Cell Environment. 2005, 28: 1046-1055.
[17] Chao Y Y, Chen C Y, Huang W D, et al. Salicylic acid-mediated hydrogen peroxide accumulation and protection against Cd toxicity in rice leaves. Plant and Soil. 2010, 329: 327-337.
[18] Nana W L, Tchameni N S, Fokom R, et al. Flavonoïd compounds synthesis by Cocoa fruits (Theobroma cacao L.) in response to Phytophthora megakarya infection. Research Journal of Agriculture and Biological Sciences. 2011, 7: 335-342.
[19] Ngonkeu M E L. 2009. Tolérance de certaines variétés de maïs aux sols à toxicité aluminique et manganique du Cameroun et diversités moléculaire et fonctionnelle des mycorhizes à arbuscules. Thèse, Université de Yaoundé I, Cameroun, 224 p.
[20] Tchameni N S, Nwaga D, Nana W L, et al. Growth enhancement, amino acid synthesis and reduction in susceptibility towards Phytophthora megakarya by arbuscular mycorrhizal fungi inoculation Cocoa plants. Journal of Phytopathology. 2012. 160: 220-228.
[21] Phillips J M, Hayman D S. Improved Procedures for Clearing Roots and Staining Parasitic Vesicular- Arbuscular Mycorrhizal Fungi for Rapid Assessment of Infection. Transactions of the British Mycological Society. 1970, 55: 158-161.
[22] Marx H D, Bryan W C, Cordell C E. Survival and growth of Pine seedlings with Pisolithus ectomy-corrhizae after two years on reforestation sites in North Carolina and Florida. Forest science. 1977, 3: 363-373.
[23] Trouvelot A, Kough J, Gianinazzi-Pearson V. Evaluation of VA infection levels in root systems. Research for estimation methods having a functional significance. In: V. Gianinazzi-Pearson and S. Gianinazzi (eds.), Physiological and Genetical Aspects of Mycorrhizae. INRA Press, Paris, France. 1986, 217–221.
[24] Nyassé S, Cilas C, Herail C, et al. Leaf inoculations as an early screening test for Cocoa (Theobroma cacao L.) resistance to Phytophthora black pod disease. Crop Protection. 1995, 14: 657-663.
[25] Coulibaly K, Kebe I B, Koffi N K, et al. Caractérisation des isolats de Phytophthora sp. du verger cacaoyers de Cote d’Ivoire. Journal of Applied. Biosciences. 2013, 70: 5567- 5579.
[26] Marigo G. Sur une méthode de fractionnement et d’estimation des composés phénoliques chez les végétaux. Analysis. 1973, 2: 106-110.
[27] Effa O P, Manga N J, Ondobo L M, et al. Virulence Test of Some Phytophthora Megakarya Isolates on Cocoa (Theobroma Cacao L.) Hybrid Pods. IOSR Journal of Biotechnology and Biochemistry. 2017, 3 (1): 73-81.
[28] Boudjeko T, Djocgoue P F, Nankeu D J, et al. Luteolin derivatives and heritability of resistance in the Theobroma cacao L. (Cacao)/Phytophthora megakarya Bras and Griff interaction. Australisian Plant Pathology. 2007, 36: 56-61.
[29] Ondobo M L, Effa O P, Djocgoue P F, et al. Phenolic content and heritability of resistance in four hybrid populations of Theobroma cacao L. after leaves inoculation with Phytophthora megakarya Bras. and Grif. International Journal of Biological and Chemical Sciences. 2014, 8 (1):17-30.
[30] Minyaka E, Simo C, Kusznierewicz B, et al. Flavones in Cocoa Defence against Phytophthora megakarya. Journal of Botanical Sciences. 2017, 6 (3): 50-63.
[31] Tchameni S N, Ngonkeu M E L, Begoude B A D, et al. Effect of Trichoderma asperellum and arbuscular mycorrhizal fungi on cacao growth and resistance against black pod disease. Crop Protection. 2011, 30: 1321-1327.
[32] Nana W L, Nwaga D, Fokom R, et al. Variation des composés phénoliques chez Vigna unguiculata (L.) Walp. (Légumineuse) et influence des rhizobia et des mycorhizes sur leur biosynthèse. African Journal of Science and Technology. 2002, 3 (2): 127-135.
[33] AL-Askar A A, Rashad Y M. Arbuscular mycorrhizal fungi: a biocontrol agent against common bean Fusarium root rot disease. Plant Pathology Journal. 2010, 9: 31-38.
[34] Lu F C, Lee C Y, Wang C L. The influence of arbuscular mycorrhizal fungi inoculation on yam (Dioscorea spp.) tuber weights and secondary metabolite content. Peer Journal. 2015, DOI: 10.7717/peerj.1266.
[35] Dihazi A, J’aity F, Zouine J, et al. Effect of Salicylic acid on phenolic compounds related to date palm resistance to Fusarium oxysporum f. sp. albedinis. Phytopathologia Mediterranea. 2003, 42 (1): 9-16.
[36] Patel M, Kothari I L, Mohan J S. Plant defense induced in vitro propagated banana (Musa paradisiaca) plantlets by Fusarium derived elicitors. Indian Journal of Experimental Biology. 2004, 42: 728-731.
[37] Arfaoui A, El Hadrami A, Mabrouk Y, et al. Treatment of chickpea with Rhizobium isolates enhances the expression of phenylpropanoid defense-related genes in response to infection by Fusarium oxysporum f. sp. Ciceris. Plant Physiology and Biochemistry. 2007, 45: 470-479.
[38] Wang X, El Hadrami A, Adam L R, et al. Differential activation and suppression of potato defence responses by Phytophthora infestans isolates representing US-1 and US-8 genotypes. Plant Patholology. 2008, 57 (6): 1026-1037.
[39] Mandal S, Mallick N, Mitra A. Salicylic acid-induced resistance to Fusarium oxysporum f. sp. lycopersici in tomato. Plant Physiology and Biochemistry. 2009, 47: 642-649.
[40] Housti F, Andary C, Gargadenne A, et al. Effects of wounding and Salicylic acid on hydroxycinnomocylmalic acid in Thumbergia alata. Plant Physiology and Biochemistry. 2002, 40: 761-769.
[41] Tania F, Juan P F, Ana I C, et al. Effect of Salicylic acid treatment on tomato plant physiology and tolerance to potato virus X infection. European Journal of Plant Pathology. 2014, 138: 331-345.
[42] Don J, Wan G, Liang Z. Accumulation of salycilic acid-induced phenolic compounds and raised activities of secondary metabolic and antioxidative enzymes in Salvia miltiorrhiza cell culture. Journal of Biotechnology. 2010, 148: 99–104.
[43] Tchameni N S, Sameza L M, O’donovanb A, et al. Antagonism of Trichoderma asperellum against Phytophthora megakarya and its potential to promote cacao growth and induce biochemical defence. Mycology. 2017, 8: 84-92.
Author Information
  • Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon; Laboratory of Plant Physiology, Department of Biological Sciences, Higher Teacher’s Training College, University of Yaoundé 1, Yaoundé, Cameroon

  • Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon; Laboratory of Plant Physiology, Department of Biological Sciences, Higher Teacher’s Training College, University of Yaoundé 1, Yaoundé, Cameroon

  • Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon

  • Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon

  • Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon

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    Simo Claude, Minyaka Emile, Tassong Saah Denis, Njonzo-nzo Stephanie Alvine, Djocgoue Pierre François, et al. (2019). Effect of Biofertilizers on the Phenolic Content in a Hybrid Family of Cacao After Leaf Infection with Phytophthora megakarya and Exogenous Application of Salicylic Acid. American Journal of Agriculture and Forestry, 7(3), 84-94. https://doi.org/10.11648/j.ajaf.20190703.11

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    ACS Style

    Simo Claude; Minyaka Emile; Tassong Saah Denis; Njonzo-nzo Stephanie Alvine; Djocgoue Pierre François, et al. Effect of Biofertilizers on the Phenolic Content in a Hybrid Family of Cacao After Leaf Infection with Phytophthora megakarya and Exogenous Application of Salicylic Acid. Am. J. Agric. For. 2019, 7(3), 84-94. doi: 10.11648/j.ajaf.20190703.11

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    AMA Style

    Simo Claude, Minyaka Emile, Tassong Saah Denis, Njonzo-nzo Stephanie Alvine, Djocgoue Pierre François, et al. Effect of Biofertilizers on the Phenolic Content in a Hybrid Family of Cacao After Leaf Infection with Phytophthora megakarya and Exogenous Application of Salicylic Acid. Am J Agric For. 2019;7(3):84-94. doi: 10.11648/j.ajaf.20190703.11

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  • @article{10.11648/j.ajaf.20190703.11,
      author = {Simo Claude and Minyaka Emile and Tassong Saah Denis and Njonzo-nzo Stephanie Alvine and Djocgoue Pierre François and Taffouo Victor Desire},
      title = {Effect of Biofertilizers on the Phenolic Content in a Hybrid Family of Cacao After Leaf Infection with Phytophthora megakarya and Exogenous Application of Salicylic Acid},
      journal = {American Journal of Agriculture and Forestry},
      volume = {7},
      number = {3},
      pages = {84-94},
      doi = {10.11648/j.ajaf.20190703.11},
      url = {https://doi.org/10.11648/j.ajaf.20190703.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajaf.20190703.11},
      abstract = {In order to protect cacao against Phytophthora megakarya, the most aggressive pathogen of this plant in Cameroon, a study was carried out on hybrid genotypes of the family F79SA of cacao (Theobroma cacao L.) to investigate the effect of inoculation of the biofertilizers Gigaspora margarita and Acaulospora tuberculata on the phenolic compound content in hybrid genotypes after leaf infection with Phytophthora megakarya and treatment of salicylic acid (SA). Thus, the phenolic compound content of hybrid genotypes of the family F79SA of T. cacao was evaluated after artificial infection of leaves with P. megakarya and treatment of salicylic acid without control and under control of biofertilizers. The artificial infection of P. megakarya and exogenous application of salicylic acid resulted in an increase in the accumulation of phenolic compounds (PC) in all genotypes. This increase was more important under the control of Gigaspora margarita and Acaulospora tuberculata and varied from one genotype to another. The PC content analysis map of these genotypes at different treatment conditions under the control of biofertilizers showed a gradual evolution of black coloration, a sign of the increase in phenolic compound content related to concentrations of salicylic acid and infected leaves in all hybrid genotypes thus expressing high tolerance. This map allowed to classify hybrid genotypes according to their level of tolerance. A negative and significant correlation (P = 0.05) was observed between the development of necrosis and the accumulation of phenolic compounds on one hand and between salicylic acid and the accumulation of phenolic compounds on the other hand. Salicylic acid can therefore be used in the cacao selection program in the absence of the pathogen for the identification of hybrid cacao genotypes as well as in other similar breeding programs.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Effect of Biofertilizers on the Phenolic Content in a Hybrid Family of Cacao After Leaf Infection with Phytophthora megakarya and Exogenous Application of Salicylic Acid
    AU  - Simo Claude
    AU  - Minyaka Emile
    AU  - Tassong Saah Denis
    AU  - Njonzo-nzo Stephanie Alvine
    AU  - Djocgoue Pierre François
    AU  - Taffouo Victor Desire
    Y1  - 2019/05/29
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ajaf.20190703.11
    DO  - 10.11648/j.ajaf.20190703.11
    T2  - American Journal of Agriculture and Forestry
    JF  - American Journal of Agriculture and Forestry
    JO  - American Journal of Agriculture and Forestry
    SP  - 84
    EP  - 94
    PB  - Science Publishing Group
    SN  - 2330-8591
    UR  - https://doi.org/10.11648/j.ajaf.20190703.11
    AB  - In order to protect cacao against Phytophthora megakarya, the most aggressive pathogen of this plant in Cameroon, a study was carried out on hybrid genotypes of the family F79SA of cacao (Theobroma cacao L.) to investigate the effect of inoculation of the biofertilizers Gigaspora margarita and Acaulospora tuberculata on the phenolic compound content in hybrid genotypes after leaf infection with Phytophthora megakarya and treatment of salicylic acid (SA). Thus, the phenolic compound content of hybrid genotypes of the family F79SA of T. cacao was evaluated after artificial infection of leaves with P. megakarya and treatment of salicylic acid without control and under control of biofertilizers. The artificial infection of P. megakarya and exogenous application of salicylic acid resulted in an increase in the accumulation of phenolic compounds (PC) in all genotypes. This increase was more important under the control of Gigaspora margarita and Acaulospora tuberculata and varied from one genotype to another. The PC content analysis map of these genotypes at different treatment conditions under the control of biofertilizers showed a gradual evolution of black coloration, a sign of the increase in phenolic compound content related to concentrations of salicylic acid and infected leaves in all hybrid genotypes thus expressing high tolerance. This map allowed to classify hybrid genotypes according to their level of tolerance. A negative and significant correlation (P = 0.05) was observed between the development of necrosis and the accumulation of phenolic compounds on one hand and between salicylic acid and the accumulation of phenolic compounds on the other hand. Salicylic acid can therefore be used in the cacao selection program in the absence of the pathogen for the identification of hybrid cacao genotypes as well as in other similar breeding programs.
    VL  - 7
    IS  - 3
    ER  - 

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