American Journal of Agriculture and Forestry

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FISH Analysis of Oryza latifolia and Oryza alta Genomes with 45S rDNA Probes

Received: 04 September 2018    Accepted: 04 October 2018    Published: 26 October 2018
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Abstract

Using Fluorescence in Situ Hybridization with 45S rDNA probes, we analyzed two wild rice species, Oryza alta and Oryza latifolia, both having CCDD genomes. The results showed that the hybridization signals of 45S rDNA in O.latifolia chromosome preparations were distributed on different chromosomes with the number of 10~16. The same signals in O.alta chromosome preparations were 6 and distributed on three pairs of homologous chromosomes: with two pairs in short chromosome arms and the other one pair in long chromosome arms. The number and positions of 45S rDNA signals are stable in O.alta genome, but there are some dynamic changes of signals in O.latifolia genome. Our observations indicated that there are distinct differences between the two CCDD genomes. Comparative analysis of the karyotype of O.alta and O.latifolia chromosomes based on the Fluorescence in Situ Hybridization pictures showed vast differences. We propose that the genome of O.alta may be differentiated earlier in evolution and tend to be stable, while O.latifolia may still be in its evolutionary process. Based on the imbalance of evolution and differences of genomic structure, we propose that O.alta and O.latifolia can be divided into two wild rice species, which may be better conforming with their evolution characteristics. The mechanism and characteristics of 45s rDNA’s distribution in the chromosomes are discussed.

DOI 10.11648/j.ajaf.20180606.12
Published in American Journal of Agriculture and Forestry (Volume 6, Issue 6, November 2018)
Page(s) 169-175
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

45S rDNA, Fluorescence in Situ Hybridization (FISH), Oryza alta, Oryza latifolia

References
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Author Information
  • Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area, Wuhan, China; School of Life Sciences, South-Central University for Nationalities, Wuhan, China

  • School of Life Sciences, South-Central University for Nationalities, Wuhan, China; The Third Middle School of Wuyi County in Zhejiang Province, Jinhua, China

  • Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area, Wuhan, China; School of Life Sciences, South-Central University for Nationalities, Wuhan, China

  • Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area, Wuhan, China; School of Life Sciences, South-Central University for Nationalities, Wuhan, China

  • Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area, Wuhan, China; School of Life Sciences, South-Central University for Nationalities, Wuhan, China

Cite This Article
  • APA Style

    Zhou Hui, Lan Weizhen, Qin Rui, Liu Hong, Li Gang. (2018). FISH Analysis of Oryza latifolia and Oryza alta Genomes with 45S rDNA Probes. American Journal of Agriculture and Forestry, 6(6), 169-175. https://doi.org/10.11648/j.ajaf.20180606.12

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    ACS Style

    Zhou Hui; Lan Weizhen; Qin Rui; Liu Hong; Li Gang. FISH Analysis of Oryza latifolia and Oryza alta Genomes with 45S rDNA Probes. Am. J. Agric. For. 2018, 6(6), 169-175. doi: 10.11648/j.ajaf.20180606.12

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    AMA Style

    Zhou Hui, Lan Weizhen, Qin Rui, Liu Hong, Li Gang. FISH Analysis of Oryza latifolia and Oryza alta Genomes with 45S rDNA Probes. Am J Agric For. 2018;6(6):169-175. doi: 10.11648/j.ajaf.20180606.12

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  • @article{10.11648/j.ajaf.20180606.12,
      author = {Zhou Hui and Lan Weizhen and Qin Rui and Liu Hong and Li Gang},
      title = {FISH Analysis of Oryza latifolia and Oryza alta Genomes with 45S rDNA Probes},
      journal = {American Journal of Agriculture and Forestry},
      volume = {6},
      number = {6},
      pages = {169-175},
      doi = {10.11648/j.ajaf.20180606.12},
      url = {https://doi.org/10.11648/j.ajaf.20180606.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajaf.20180606.12},
      abstract = {Using Fluorescence in Situ Hybridization with 45S rDNA probes, we analyzed two wild rice species, Oryza alta and Oryza latifolia, both having CCDD genomes. The results showed that the hybridization signals of 45S rDNA in O.latifolia chromosome preparations were distributed on different chromosomes with the number of 10~16. The same signals in O.alta chromosome preparations were 6 and distributed on three pairs of homologous chromosomes: with two pairs in short chromosome arms and the other one pair in long chromosome arms. The number and positions of 45S rDNA signals are stable in O.alta genome, but there are some dynamic changes of signals in O.latifolia genome. Our observations indicated that there are distinct differences between the two CCDD genomes. Comparative analysis of the karyotype of O.alta and O.latifolia chromosomes based on the Fluorescence in Situ Hybridization pictures showed vast differences. We propose that the genome of O.alta may be differentiated earlier in evolution and tend to be stable, while O.latifolia may still be in its evolutionary process. Based on the imbalance of evolution and differences of genomic structure, we propose that O.alta and O.latifolia can be divided into two wild rice species, which may be better conforming with their evolution characteristics. The mechanism and characteristics of 45s rDNA’s distribution in the chromosomes are discussed.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - FISH Analysis of Oryza latifolia and Oryza alta Genomes with 45S rDNA Probes
    AU  - Zhou Hui
    AU  - Lan Weizhen
    AU  - Qin Rui
    AU  - Liu Hong
    AU  - Li Gang
    Y1  - 2018/10/26
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ajaf.20180606.12
    DO  - 10.11648/j.ajaf.20180606.12
    T2  - American Journal of Agriculture and Forestry
    JF  - American Journal of Agriculture and Forestry
    JO  - American Journal of Agriculture and Forestry
    SP  - 169
    EP  - 175
    PB  - Science Publishing Group
    SN  - 2330-8591
    UR  - https://doi.org/10.11648/j.ajaf.20180606.12
    AB  - Using Fluorescence in Situ Hybridization with 45S rDNA probes, we analyzed two wild rice species, Oryza alta and Oryza latifolia, both having CCDD genomes. The results showed that the hybridization signals of 45S rDNA in O.latifolia chromosome preparations were distributed on different chromosomes with the number of 10~16. The same signals in O.alta chromosome preparations were 6 and distributed on three pairs of homologous chromosomes: with two pairs in short chromosome arms and the other one pair in long chromosome arms. The number and positions of 45S rDNA signals are stable in O.alta genome, but there are some dynamic changes of signals in O.latifolia genome. Our observations indicated that there are distinct differences between the two CCDD genomes. Comparative analysis of the karyotype of O.alta and O.latifolia chromosomes based on the Fluorescence in Situ Hybridization pictures showed vast differences. We propose that the genome of O.alta may be differentiated earlier in evolution and tend to be stable, while O.latifolia may still be in its evolutionary process. Based on the imbalance of evolution and differences of genomic structure, we propose that O.alta and O.latifolia can be divided into two wild rice species, which may be better conforming with their evolution characteristics. The mechanism and characteristics of 45s rDNA’s distribution in the chromosomes are discussed.
    VL  - 6
    IS  - 6
    ER  - 

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