Grafting shaping the Microbial Community Structure to Suppress <i>Verticillium dahliae</i> in the Rhizosphere of Eggplants

Yuling Yin; Shaochun Luo; Yunpeng Li; Jinsong Zhou; Yongping Tang; Yuan Liu

doi:doi:10.11648/j.ajaf.20180605.13

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Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants

Yuling Yin, Shaochun Luo, Yunpeng Li, Jinsong Zhou, Yongping Tang, Yuan Liu

Published in American Journal of Agriculture and Forestry (Volume 6, Issue 5)

Received: 7 May 2018 Accepted: 7 August 2018 Published: 15 September 2018

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Abstract

This study evaluated that grafting as an effective measure on resistance disease in continuous cropping system has a remarkable impact on improving the microbial community population and structure conditions in the rhizosphere of eggplant, which lead to a highly suppressive on the disease incidence. Verticillium dahliae, microbial community and soil enzyme activities in the rhizosphere of both grafted and self-rooted eggplants investigations were conducted in different eggplant growth stages in pot trials to determine the effects of grafting on the soil microbiological properties. In addition, an assessment of the bio-control effects of antagonistic microbes isolated from rhizosphere of grafted eggplants on the incidence of Verticillium wilt was performed. Grafted eggplants exhibited strong resistance (nearly 90%) with a lower pathological incident index. Amounts of Verticillium dahliae in the rhizosphere of grafted eggplants were lower before the fruiting stage compared to non-grafted self-rooting eggplants. Although each general classification of microbes (bacteria, actinomyces and fungi) was reduced over all growth stages by grafting, the ranges of bacterial and actinomycete reduction in the rhizosphere of grafted eggplants were lower compared to self-rooted eggplants. Experimental evidence indicated that grafting increased the relative ratios of Bacteria to Fungi and Actinomycete to Fungi. In terms of functional microbes, amounts of azotobacteria, ammonifying and nitrifying bacteria were significantly higher in grafted eggplants than self-rooted eggplants, especially after floral initiation. Compared to Control, grafting leaded to a significantly positive impacts on invertase, protein enzyme, urease, peroxidase and catalase activities in the rhizosphere of eggplants. Using antagonistic strains have showed strong suppression of Verticillium dahliae growth to study the prevention and curative effects on Verticillium wilt in pot trials.

Published in	American Journal of Agriculture and Forestry (Volume 6, Issue 5)
DOI	10.11648/j.ajaf.20180605.13
Page(s)	132-140
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Grafting Eggplants, Verticillium Wilt, Verticillium dahliae, Continuous Cropping, Soil Microorganism

References

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APA Style

Yuling Yin, Shaochun Luo, Yunpeng Li, Jinsong Zhou, Yongping Tang, et al. (2018). Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants. American Journal of Agriculture and Forestry, 6(5), 132-140. https://doi.org/10.11648/j.ajaf.20180605.13

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ACS Style

Yuling Yin; Shaochun Luo; Yunpeng Li; Jinsong Zhou; Yongping Tang, et al. Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants. Am. J. Agric. For. 2018, 6(5), 132-140. doi: 10.11648/j.ajaf.20180605.13

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AMA Style

Yuling Yin, Shaochun Luo, Yunpeng Li, Jinsong Zhou, Yongping Tang, et al. Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants. Am J Agric For. 2018;6(5):132-140. doi: 10.11648/j.ajaf.20180605.13

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@article{10.11648/j.ajaf.20180605.13,
  author = {Yuling Yin and Shaochun Luo and Yunpeng Li and Jinsong Zhou and Yongping Tang and Yuan Liu},
  title = {Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants},
  journal = {American Journal of Agriculture and Forestry},
  volume = {6},
  number = {5},
  pages = {132-140},
  doi = {10.11648/j.ajaf.20180605.13},
  url = {https://doi.org/10.11648/j.ajaf.20180605.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20180605.13},
  abstract = {This study evaluated that grafting as an effective measure on resistance disease in continuous cropping system has a remarkable impact on improving the microbial community population and structure conditions in the rhizosphere of eggplant, which lead to a highly suppressive on the disease incidence. Verticillium dahliae, microbial community and soil enzyme activities in the rhizosphere of both grafted and self-rooted eggplants investigations were conducted in different eggplant growth stages in pot trials to determine the effects of grafting on the soil microbiological properties. In addition, an assessment of the bio-control effects of antagonistic microbes isolated from rhizosphere of grafted eggplants on the incidence of Verticillium wilt was performed. Grafted eggplants exhibited strong resistance (nearly 90%) with a lower pathological incident index. Amounts of Verticillium dahliae in the rhizosphere of grafted eggplants were lower before the fruiting stage compared to non-grafted self-rooting eggplants. Although each general classification of microbes (bacteria, actinomyces and fungi) was reduced over all growth stages by grafting, the ranges of bacterial and actinomycete reduction in the rhizosphere of grafted eggplants were lower compared to self-rooted eggplants. Experimental evidence indicated that grafting increased the relative ratios of Bacteria to Fungi and Actinomycete to Fungi. In terms of functional microbes, amounts of azotobacteria, ammonifying and nitrifying bacteria were significantly higher in grafted eggplants than self-rooted eggplants, especially after floral initiation. Compared to Control, grafting leaded to a significantly positive impacts on invertase, protein enzyme, urease, peroxidase and catalase activities in the rhizosphere of eggplants. Using antagonistic strains have showed strong suppression of Verticillium dahliae growth to study the prevention and curative effects on Verticillium wilt in pot trials.},
 year = {2018}
}

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TY  - JOUR
T1  - Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants
AU  - Yuling Yin
AU  - Shaochun Luo
AU  - Yunpeng Li
AU  - Jinsong Zhou
AU  - Yongping Tang
AU  - Yuan Liu
Y1  - 2018/09/15
PY  - 2018
N1  - https://doi.org/10.11648/j.ajaf.20180605.13
DO  - 10.11648/j.ajaf.20180605.13
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 132
EP  - 140
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20180605.13
AB  - This study evaluated that grafting as an effective measure on resistance disease in continuous cropping system has a remarkable impact on improving the microbial community population and structure conditions in the rhizosphere of eggplant, which lead to a highly suppressive on the disease incidence. Verticillium dahliae, microbial community and soil enzyme activities in the rhizosphere of both grafted and self-rooted eggplants investigations were conducted in different eggplant growth stages in pot trials to determine the effects of grafting on the soil microbiological properties. In addition, an assessment of the bio-control effects of antagonistic microbes isolated from rhizosphere of grafted eggplants on the incidence of Verticillium wilt was performed. Grafted eggplants exhibited strong resistance (nearly 90%) with a lower pathological incident index. Amounts of Verticillium dahliae in the rhizosphere of grafted eggplants were lower before the fruiting stage compared to non-grafted self-rooting eggplants. Although each general classification of microbes (bacteria, actinomyces and fungi) was reduced over all growth stages by grafting, the ranges of bacterial and actinomycete reduction in the rhizosphere of grafted eggplants were lower compared to self-rooted eggplants. Experimental evidence indicated that grafting increased the relative ratios of Bacteria to Fungi and Actinomycete to Fungi. In terms of functional microbes, amounts of azotobacteria, ammonifying and nitrifying bacteria were significantly higher in grafted eggplants than self-rooted eggplants, especially after floral initiation. Compared to Control, grafting leaded to a significantly positive impacts on invertase, protein enzyme, urease, peroxidase and catalase activities in the rhizosphere of eggplants. Using antagonistic strains have showed strong suppression of Verticillium dahliae growth to study the prevention and curative effects on Verticillium wilt in pot trials.
VL  - 6
IS  - 5
ER  -

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Author Information

Yuling Yin

Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural Sciences, Nanchang, China
Shaochun Luo

Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural Sciences, Nanchang, China
Yunpeng Li

College of Agricultural Resources and Environment, Heilongjiang University, Harbin, China
Jinsong Zhou

Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural Sciences, Nanchang, China
Yongping Tang

Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural Sciences, Nanchang, China
Yuan Liu

Liaoning Saline or Alkaline Land Utilization and Research Institute, Liaoning Academy of Agricultural Sciences, Panjin, China

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APA Style

Yuling Yin, Shaochun Luo, Yunpeng Li, Jinsong Zhou, Yongping Tang, et al. (2018). Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants. American Journal of Agriculture and Forestry, 6(5), 132-140. https://doi.org/10.11648/j.ajaf.20180605.13

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ACS Style

Yuling Yin; Shaochun Luo; Yunpeng Li; Jinsong Zhou; Yongping Tang, et al. Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants. Am. J. Agric. For. 2018, 6(5), 132-140. doi: 10.11648/j.ajaf.20180605.13

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AMA Style

Yuling Yin, Shaochun Luo, Yunpeng Li, Jinsong Zhou, Yongping Tang, et al. Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants. Am J Agric For. 2018;6(5):132-140. doi: 10.11648/j.ajaf.20180605.13

Copy | Download

@article{10.11648/j.ajaf.20180605.13,
  author = {Yuling Yin and Shaochun Luo and Yunpeng Li and Jinsong Zhou and Yongping Tang and Yuan Liu},
  title = {Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants},
  journal = {American Journal of Agriculture and Forestry},
  volume = {6},
  number = {5},
  pages = {132-140},
  doi = {10.11648/j.ajaf.20180605.13},
  url = {https://doi.org/10.11648/j.ajaf.20180605.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20180605.13},
  abstract = {This study evaluated that grafting as an effective measure on resistance disease in continuous cropping system has a remarkable impact on improving the microbial community population and structure conditions in the rhizosphere of eggplant, which lead to a highly suppressive on the disease incidence. Verticillium dahliae, microbial community and soil enzyme activities in the rhizosphere of both grafted and self-rooted eggplants investigations were conducted in different eggplant growth stages in pot trials to determine the effects of grafting on the soil microbiological properties. In addition, an assessment of the bio-control effects of antagonistic microbes isolated from rhizosphere of grafted eggplants on the incidence of Verticillium wilt was performed. Grafted eggplants exhibited strong resistance (nearly 90%) with a lower pathological incident index. Amounts of Verticillium dahliae in the rhizosphere of grafted eggplants were lower before the fruiting stage compared to non-grafted self-rooting eggplants. Although each general classification of microbes (bacteria, actinomyces and fungi) was reduced over all growth stages by grafting, the ranges of bacterial and actinomycete reduction in the rhizosphere of grafted eggplants were lower compared to self-rooted eggplants. Experimental evidence indicated that grafting increased the relative ratios of Bacteria to Fungi and Actinomycete to Fungi. In terms of functional microbes, amounts of azotobacteria, ammonifying and nitrifying bacteria were significantly higher in grafted eggplants than self-rooted eggplants, especially after floral initiation. Compared to Control, grafting leaded to a significantly positive impacts on invertase, protein enzyme, urease, peroxidase and catalase activities in the rhizosphere of eggplants. Using antagonistic strains have showed strong suppression of Verticillium dahliae growth to study the prevention and curative effects on Verticillium wilt in pot trials.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants
AU  - Yuling Yin
AU  - Shaochun Luo
AU  - Yunpeng Li
AU  - Jinsong Zhou
AU  - Yongping Tang
AU  - Yuan Liu
Y1  - 2018/09/15
PY  - 2018
N1  - https://doi.org/10.11648/j.ajaf.20180605.13
DO  - 10.11648/j.ajaf.20180605.13
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 132
EP  - 140
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20180605.13
AB  - This study evaluated that grafting as an effective measure on resistance disease in continuous cropping system has a remarkable impact on improving the microbial community population and structure conditions in the rhizosphere of eggplant, which lead to a highly suppressive on the disease incidence. Verticillium dahliae, microbial community and soil enzyme activities in the rhizosphere of both grafted and self-rooted eggplants investigations were conducted in different eggplant growth stages in pot trials to determine the effects of grafting on the soil microbiological properties. In addition, an assessment of the bio-control effects of antagonistic microbes isolated from rhizosphere of grafted eggplants on the incidence of Verticillium wilt was performed. Grafted eggplants exhibited strong resistance (nearly 90%) with a lower pathological incident index. Amounts of Verticillium dahliae in the rhizosphere of grafted eggplants were lower before the fruiting stage compared to non-grafted self-rooting eggplants. Although each general classification of microbes (bacteria, actinomyces and fungi) was reduced over all growth stages by grafting, the ranges of bacterial and actinomycete reduction in the rhizosphere of grafted eggplants were lower compared to self-rooted eggplants. Experimental evidence indicated that grafting increased the relative ratios of Bacteria to Fungi and Actinomycete to Fungi. In terms of functional microbes, amounts of azotobacteria, ammonifying and nitrifying bacteria were significantly higher in grafted eggplants than self-rooted eggplants, especially after floral initiation. Compared to Control, grafting leaded to a significantly positive impacts on invertase, protein enzyme, urease, peroxidase and catalase activities in the rhizosphere of eggplants. Using antagonistic strains have showed strong suppression of Verticillium dahliae growth to study the prevention and curative effects on Verticillium wilt in pot trials.
VL  - 6
IS  - 5
ER  -

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