Functional Characterization of a WD-Repeat Protein Gene (<i>OsWD1</i>) in Rice

Siou-Luan He; Shin-Lon Ho

doi:doi:10.11648/j.ajaf.20180602.11

| Peer-Reviewed

Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice

Siou-Luan He, Shin-Lon Ho

Published in American Journal of Agriculture and Forestry (Volume 6, Issue 2)

Received: 7 April 2018 Accepted: 23 April 2018 Published: 11 May 2018

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

A gene encoded WD-40 repeat protein (OsWD1) was isolated from rice cells to study the platform for protein-protein interactions in germinating seeds. The expression of OsWD1 was found to be specifically induced at 3 d of seed germination. By ectopic overexpressing (OsWD1-Ox) or RNA interference gene knockdown (OsWD1-Ri) of OsWD1, respectively. Results revealed that the rice seedlings and grain size were enhanced in OsWD1-Ox lines, but were reduced in OsWD1-Ri lines. Moreover, the expression of GA-inducible gene, OsEP3A, which was strongly induced in OsWD1-Ox lines during rice seed germination. By iodine staining in starch containing solid medium, the a-amylase activities were further enhanced in OsWD1-Ox lines regardless with or without exogenous GA in the medium. These results suggested that OsWD1 might acts as a platform for protein-protein interactions to regulate seedling and seed development in rice by a GA-dependent manner.

Published in	American Journal of Agriculture and Forestry (Volume 6, Issue 2)
DOI	10.11648/j.ajaf.20180602.11
Page(s)	18-27
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Rice, OsWD1, Overexpression, Plant Height, Grain Size, Gibberellin Acid

References

[1]	E. J. Neer, C. J. Schmidt, R. Nambudripad, T. F. Smith “The ancient regulatory-protein family of WD-repeat proteins” Nature, vol. 371, pp. 297-300. 1994.
[2]	S. van Nocker, P. Ludwig “The WD-repeat protein superfamily in Arabidopsis: conservation and divergence in structure and function” BMC Genomics, vol. 4, 50. 2003.
[3]	J. Guo, J. G. Chen “RACK1 genes regulate plant development with unequal genetic redundancy in Arabidopsis” BMC Plant Biol, vol. 8, 108; doi:10.1186/1471-2229-8-108. 2008.
[4]	E. A. Ananieva, G. E. Gillaspy, A. Ely, R. N. Burnette, F. L. Erickson “Interaction of the WD40 domain of a myoinositol polyphosphate 5-phosphatase with SnRK1 links inositol, sugar, and stress signaling” Plant Physiol, vol. 148, pp.1868-1882. 2008.
[5]	T. F. Smith, C. Gaitatzes, K. Saxena, E. J. Neer “The WD repeat: a common architecture for diverse functions” Trends Biochem Sci, vol. 24, pp. 181-185. 1999.
[6]	Y. Miyata, T. Shibata, M. Aoshima, T. Tsubata, E. Nishida “The molecular chaperone TRiC/CCT binds to the Trp-Asp 40 (WD40) repeat protein WDR68 and promotes its folding, protein kinase DYRK1A binding, and nuclear accumulation” J Biol Chem, vol. 289, pp. 33320-33332. 2014.
[7]	J. C. Miller, W. R. Chezem, N. K. Clay “Ternary WD40 repeat-containing protein complexes: evolution, composition and roles in plant immunity” Front Plant Sci, vol. 6, 1108. 2016.
[8]	X. Xu, I. Paik, L. Zhu, Q. Bu, X. Huang, X. W. Deng, E. Huq “PHYTOCHROME INTERACTING FACTOR1 enhances the E3 ligase activity of CONSTITUTIVE PHOTOMORPHOGENIC1 to synergistically repress photomorphogenesis in Arabidopsis” Plant Cell, vol. 26, pp. 1992-2006. 2014.
[9]	K. A. Lease, J. Wen, J. Li, J. T. Doke, E. Liscum, J. C. Walker “A mutant Arabidopsis heterotrimeric G-protein b subunit affects leaf, flower, and fruit development” Plant Cell, vol. 13, pp. 2631-2641. 2001.
[10]	S. Hoppins, L. Lackner, J. Nunnari “The machines that divide and fuse mitochondria” Annu Rev Biochem, vol. 76, pp. 751-780. 2007.
[11]	Z. Ben-Simhon, S. Judeinstein, T. Nadler-Hassar, T. Trainin, I. Bar-Ya’akov, H. Borochov-Neori, D. Holland “A pomegranate (Punica granatum L.) WD40-repeat gene is a functional homologue of Arabidopsis TTG1and is involved in the regulation of anthocyanin biosynthesis during pomegranate fruit development” Planta, vol. 234, pp. 865-881. 2011.
[12]	I. Mascheretti, R. Battaglia, D. Mainieri, A. Altana, M. Lauria, V. Rossi “The WD40-repeat proteins NFC101 and NFC102 regulate different aspects of maize development through chromatin modification” Plant Cell, vol. 25, pp. 404-420. 2013.
[13]	X. W. Deng, M. Matsui, N. Wei, D. Wagner, A. M. Chu, K. A. Feldmann, P. H. Quail “COP1, an Arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a Gb homologous domain” Cell, vol. 7, pp. 791-801. 1992.
[14]	T. W. McNellis, K. U. Torii, X. W. Deng “Expression of an N-terminal fragment of COP1 confers a dominant-negative effect on light-regulated seedling development in Arabidopsis” Plant Cell, vol. 8, pp. 1491-1503. 1996.
[15]	S. V. Nocker, P. Ludwig “The WD-repeat protein superfamily in Arabidopsis: conservation and divergence in structure and function” BMC Genomics, vol. 4, 50. 2003.
[16]	R. A. Ach, P. Taranto, W. Gruissem “A conserved family of WD-40 proteins binds to the retinoblastoma protein in both plants and animals” Plant Cell, vol. 9, pp. 1595-1606. 1997.
[17]	Y. W. Quian, Y. C. J. Wang, R. E. Hollingsworth, D. Jones, N. Ling, E. Y. H. P. Lee “A retinoblastoma-binding protein related to a negative regulator of Ras in yeast” Nature, vol. 364, pp. 648-652. 1993.
[18]	Y. W. Quian, E. Y. H. P. Lee “Dual retinoblastoma-binding proteins with properties related to a negative regulator of Ras in yeast” J Biol Chem, vol. 270, pp. 25507-25513. 1995.
[19]	R. Ruggieri, K. Tanaka, M. Nakafuku, Y. Kaziro, E. A. Toh, K. Matsumoto “MSI1, a negative regulator of the RAS-cAMP pathway in Saccharomyces cerevisiae” Proc Natl Acad Sci USA, vol. 86, pp. 8778-8782. 1989.
[20]	M. Ohnacker, S. M. L. Barabino, P. J. Preker, W. Keller “The WD-repeat protein Pfs2p bridges two essential factors within the yeast pre-mRNA 3’-end-processing complex” EMBO J, vol. 19, pp. 37-47. 2000.
[21]	R. P. Bhalerao, K. Salchert, L. Bakó, L. Ökrész, L. Szabados, T. Muranaka, Y. Machida, J. Schell, C. Koncz “Regulatory interaction of PRL1 WD protein with Arabidopsis SNF1-like protein kinases” Proc Natl Acad Sci USA, vol. 96, pp. 5322-5327. 1999.
[22]	K. Sompornpailin, Y. Makita, M. Yamazaki, K. Saito “A WD-repeat-containing putative regulatory protein in anthocyanin biosynthesis in Perilla frutescens” Plant Mol Biol, vol. 50, pp. 485-495. 2002.
[23]	Q. Tieu, V. Okreglak, K. Naylor, J. Nunnari “The WD repeat protein, Mdv1p, functions as a molecular adaptor by interacting with Dnm1p and Fis1p during mitochondrial fission” J Cell Biol, vol. 158, pp. 445-452. 2002.
[24]	Y. Ouyang, X. Huang, Z. Lu, J. Yao, Ouyang et al. “Genomic survey, expression profile and co-expression network analysis of OsWD40 family in rice” BMC Genomics, vol. 13, 100. 2012.
[25]	X. Gao, Z. Chen, J. Zhang, X. Li, G. Chen, X. Li, C. Wu “OsLIS-L1 encoding a lissencephaly type-1-like protein with WD40 repeats is required for plant height and male gametophyte formation in rice” Planta, vol. 235, pp. 713-727. 2012.
[26]	Y. Kwon, S. I. Yu, J. H. Park, Y. Li, J. H. Han, H. Alavilli, J. I. Cho, T. H. Kim, J. S. Jeon, B. H. Lee “OsREL2, a rice TOPLESS homolog functions in axillary meristem development in rice inflorescence” Plant Biotechnol Rep, vol. 6, pp. 213-224. 2012.
[27]	A. Yoshida, Y. Ohmori, H. Kitano, F. Taguchi-Shiobara, H. Y. Hirano “ABERRANT SPIKELET AND PANICLE1, encoding a TOPLESS-related transcriptional co-repressor, is involved in the regulation of meristem fate in rice” Plant J, vol. 70, pp. 327-33. 2012.
[28]	T. Murashige, F. Skoog “A revised medium for rapid growth and bioassays with tobacco tissue cultures” Physiol Plant, vol. 15, pp. 473-497. 1962.
[29]	W. B. Bruce, A. H. Christensen, T. Klein, M. Fromm, P. H. Quail “Photoregulation of a phytochrome gene promoter from oat transferred into rice by particle bombardment” Proc Natl Acad Sci USA, vol. 86, pp. 9692-9696. 1989.
[30]	S. L. Ho, W. F. Tong, S. M. Yu “Multiple mode regulation of a cysteine proteinase gene expression in rice” Plant Physiol, vol. 122, pp. 57-66. 2000.
[31]	S. L. Ho, Y. H. Chao, W. F. Tong, S. M. Yu “Sugar differenti al and coordinately regulates gene expression via a complex signal transduction network and multiple control mechanisms” Plant Physiol, vol. 125, pp. 877-890. 2001.
[32]	A. Marchler-Bauer, J. B. Anderson, C. DeWeese-Scott, N. D. Fedorova, L. Y. Geer, et al. “CDD: a curated Entrez database of conserved domain alignments” Nucleic Acids Res, vol. 31, pp. 383-387. 2003.
[33]	I. Letunic, L. Goodstadt, N. J. Dickens, T. Doerks, J. Schultz, R. Mott, F. Ciccarelli, R. R. Copley, C. P. Ponting, P. Bork “Recent improvements to the SMART domain-based sequence annotation resource” Nucleic Acids Res, vol. 30, pp. 242-244. 2002.
[34]	Z. N. Yang, X. R. Ye, J. Molina, M. L. Roose, T. E. Mirkov “Sequence analysis of a 282-kilobase region surrounding the citrus tristeza virus resistance gene (Ctv) locus in Poncirus trifoliate L. Raf” Plant Physiol, vol. 131, pp. 482-492. 2003.
[35]	K. Gomi, M. Matsuoka “Gibberellin signalling pathway” Curr Opin Plant Biol, vol. 6, pp. 489-493. 2003.
[36]	H. Itoh, M. Matsuoka, C. M. Steber “A role for the ubiquitin – 26S-proteasome pathway in gibberellins signaling” Trends Plant Sci, vol. 8, pp. 492-497. 2003.
[37]	S. Pattanaik, B. Patra, S. K. Singh, L. Yuan “An overview of the gene regulatory network controlling trichome development in the model plant, Arabidopsis” Front Plant Sci, vol. 5, 259. 2014.
[38]	R. P. Pharis, R. W. King “Gibberellins and reproductive development in seed plants” Annu Rev Plant Physiol, vol. 36, pp. 517-568. 1985.
[39]	S. M. Swain, J. B. Reid, Y. Kamiya “Gibberellins are required for embryo and seed development in pea” Plant J, vol. 12, pp. 1329-1338. 1997.
[40]	R. W. King, L. T. Evans “Gibberellins and flowering of grasses and cereals” Annu Rev Plant Biol, vol. 54, pp. 307-328. 2003.
[41]	J. A. Long, C. Ohno, Z. R. Smith, E. M. Meyerowitz “TOPLESS regulates apical embryonic fate in Arabidopsis” Science, vol. 312, pp. 1520-1523. 2006.
[42]	Z. Liu, V. Karmarkar “Groucho/Tup1 family co-repressors in plant development” Trends Plant Sci, vol. 13, pp. 137-144. 2008.
[43]	H. Szemenyei, M. Hannon, J. A. Long “TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis” Science, vol. 319, pp. 1384-1386. 2008.
[44]	L. Pauwels, et al. “NINJA connects the co-repressor TOPLESS to jasmonate signaling” Nature, vol. 464, pp. 788-791. 2010.
[45]	B. Causier, M. Ashworth, W. Guo, B. Davies “The TOPLESS interactome: A framework for gene repression in Arabidopsis” Plant Physiol, vol. 158, pp. 423-438. 2012.
[46]	Q. Tao, D. Guo, B. Wei, F. Zhang, C. Pang, H. Jiang, J. Zhang, T. Wei, H. Gu, L. J. Qu, G. Qina “The TIE1 transcriptional repressor links TCP transcription factors with TOPLESS/TOPLESS-RELATED corepressors and modulates leaf development in Arabidopsis” Plant Cell, vol. 25, pp. 421-437. 2013.
[47]	J. Fukazawa, H. Teramura, S. Murakoshi, K. Nasuno, N. Nishida, T. Ito, M. Yoshida, Y. Kamiya, S. Yamaguchi, Y. Takahashia “DELLAs function as coactivators of GAI-ASSOCIATED FACTOR1 in regulation of gibberellin homeostasis and signaling in Arabidopsis” Plant Cell, vol. 26, pp. 2920-2938. 2014.
[48]	J. Fukazawa, T. Ito, Y. Kamiya, S. Yamaguchi, Y. Takahashi “Binding of GID1 to DELLAs promotes dissociation of GAF1 from DELLA in GA dependent manner” Plant Signal Behav, vol. 10, 10, e1052923. 2015.
[49]	T. P. Sun, F. Gubler “Molecular mechanism of gibberellins signaling in plants” Annu Rev Plant Biol, vol. 55, pp. 197-223. 2004.

Cite This Article

Plain Text BibTeX RIS

APA Style

Siou-Luan He, Shin-Lon Ho. (2018). Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice. American Journal of Agriculture and Forestry, 6(2), 18-27. https://doi.org/10.11648/j.ajaf.20180602.11

Copy | Download

ACS Style

Siou-Luan He; Shin-Lon Ho. Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice. Am. J. Agric. For. 2018, 6(2), 18-27. doi: 10.11648/j.ajaf.20180602.11

Copy | Download

AMA Style

Siou-Luan He, Shin-Lon Ho. Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice. Am J Agric For. 2018;6(2):18-27. doi: 10.11648/j.ajaf.20180602.11

Copy | Download

@article{10.11648/j.ajaf.20180602.11,
  author = {Siou-Luan He and Shin-Lon Ho},
  title = {Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice},
  journal = {American Journal of Agriculture and Forestry},
  volume = {6},
  number = {2},
  pages = {18-27},
  doi = {10.11648/j.ajaf.20180602.11},
  url = {https://doi.org/10.11648/j.ajaf.20180602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20180602.11},
  abstract = {A gene encoded WD-40 repeat protein (OsWD1) was isolated from rice cells to study the platform for protein-protein interactions in germinating seeds. The expression of OsWD1 was found to be specifically induced at 3 d of seed germination. By ectopic overexpressing (OsWD1-Ox) or RNA interference gene knockdown (OsWD1-Ri) of OsWD1, respectively. Results revealed that the rice seedlings and grain size were enhanced in OsWD1-Ox lines, but were reduced in OsWD1-Ri lines. Moreover, the expression of GA-inducible gene, OsEP3A, which was strongly induced in OsWD1-Ox lines during rice seed germination. By iodine staining in starch containing solid medium, the a-amylase activities were further enhanced in OsWD1-Ox lines regardless with or without exogenous GA in the medium. These results suggested that OsWD1 might acts as a platform for protein-protein interactions to regulate seedling and seed development in rice by a GA-dependent manner.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice
AU  - Siou-Luan He
AU  - Shin-Lon Ho
Y1  - 2018/05/11
PY  - 2018
N1  - https://doi.org/10.11648/j.ajaf.20180602.11
DO  - 10.11648/j.ajaf.20180602.11
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 18
EP  - 27
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20180602.11
AB  - A gene encoded WD-40 repeat protein (OsWD1) was isolated from rice cells to study the platform for protein-protein interactions in germinating seeds. The expression of OsWD1 was found to be specifically induced at 3 d of seed germination. By ectopic overexpressing (OsWD1-Ox) or RNA interference gene knockdown (OsWD1-Ri) of OsWD1, respectively. Results revealed that the rice seedlings and grain size were enhanced in OsWD1-Ox lines, but were reduced in OsWD1-Ri lines. Moreover, the expression of GA-inducible gene, OsEP3A, which was strongly induced in OsWD1-Ox lines during rice seed germination. By iodine staining in starch containing solid medium, the a-amylase activities were further enhanced in OsWD1-Ox lines regardless with or without exogenous GA in the medium. These results suggested that OsWD1 might acts as a platform for protein-protein interactions to regulate seedling and seed development in rice by a GA-dependent manner.
VL  - 6
IS  - 2
ER  -

Copy | Download

Author Information

Siou-Luan He

Department of Agronomy, National Chiayi University, Chiayi, Taiwan, R.O.C.
Shin-Lon Ho

Department of Agronomy, National Chiayi University, Chiayi, Taiwan, R.O.C.

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Siou-Luan He, Shin-Lon Ho. (2018). Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice. American Journal of Agriculture and Forestry, 6(2), 18-27. https://doi.org/10.11648/j.ajaf.20180602.11

Copy | Download

ACS Style

Siou-Luan He; Shin-Lon Ho. Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice. Am. J. Agric. For. 2018, 6(2), 18-27. doi: 10.11648/j.ajaf.20180602.11

Copy | Download

AMA Style

Siou-Luan He, Shin-Lon Ho. Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice. Am J Agric For. 2018;6(2):18-27. doi: 10.11648/j.ajaf.20180602.11

Copy | Download

@article{10.11648/j.ajaf.20180602.11,
  author = {Siou-Luan He and Shin-Lon Ho},
  title = {Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice},
  journal = {American Journal of Agriculture and Forestry},
  volume = {6},
  number = {2},
  pages = {18-27},
  doi = {10.11648/j.ajaf.20180602.11},
  url = {https://doi.org/10.11648/j.ajaf.20180602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20180602.11},
  abstract = {A gene encoded WD-40 repeat protein (OsWD1) was isolated from rice cells to study the platform for protein-protein interactions in germinating seeds. The expression of OsWD1 was found to be specifically induced at 3 d of seed germination. By ectopic overexpressing (OsWD1-Ox) or RNA interference gene knockdown (OsWD1-Ri) of OsWD1, respectively. Results revealed that the rice seedlings and grain size were enhanced in OsWD1-Ox lines, but were reduced in OsWD1-Ri lines. Moreover, the expression of GA-inducible gene, OsEP3A, which was strongly induced in OsWD1-Ox lines during rice seed germination. By iodine staining in starch containing solid medium, the a-amylase activities were further enhanced in OsWD1-Ox lines regardless with or without exogenous GA in the medium. These results suggested that OsWD1 might acts as a platform for protein-protein interactions to regulate seedling and seed development in rice by a GA-dependent manner.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice
AU  - Siou-Luan He
AU  - Shin-Lon Ho
Y1  - 2018/05/11
PY  - 2018
N1  - https://doi.org/10.11648/j.ajaf.20180602.11
DO  - 10.11648/j.ajaf.20180602.11
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 18
EP  - 27
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20180602.11
AB  - A gene encoded WD-40 repeat protein (OsWD1) was isolated from rice cells to study the platform for protein-protein interactions in germinating seeds. The expression of OsWD1 was found to be specifically induced at 3 d of seed germination. By ectopic overexpressing (OsWD1-Ox) or RNA interference gene knockdown (OsWD1-Ri) of OsWD1, respectively. Results revealed that the rice seedlings and grain size were enhanced in OsWD1-Ox lines, but were reduced in OsWD1-Ri lines. Moreover, the expression of GA-inducible gene, OsEP3A, which was strongly induced in OsWD1-Ox lines during rice seed germination. By iodine staining in starch containing solid medium, the a-amylase activities were further enhanced in OsWD1-Ox lines regardless with or without exogenous GA in the medium. These results suggested that OsWD1 might acts as a platform for protein-protein interactions to regulate seedling and seed development in rice by a GA-dependent manner.
VL  - 6
IS  - 2
ER  -

Copy | Download