American Journal of Agriculture and Forestry

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Genetic Processes of Iron and Zinc Accumulation in Edible Portion of Crops and Their Agro-Biofortification: A Review

Received: 29 January 2017    Accepted: 18 April 2017    Published: 17 May 2017
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Abstract

Iron (Fe) and zinc (Zn) are essential micronutrient for both human and plants, but Fe and Zn deficiency is prevalent in the world especially developing countries including India and China. Biofortification is considered the most promising approach to alleviate Fe and Zn malnutrition. Thus this study was mainly conducted to review the recent progresses on the strategies of the processes affecting Fe and Zn accumulation in edible portion of crops at genetic and physiological levels. While agricultural approaches are useful to gain Fe and Zn enriched cereals, therefore agro-biofortification of Fe and Zn by agricultural approaches was also reviewed for possible solution in intensive agriculture system.

DOI 10.11648/j.ajaf.20170503.15
Published in American Journal of Agriculture and Forestry (Volume 5, Issue 3, May 2017)
Page(s) 65-72
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Iron, Zinc, Gene, Homeostasis, Agro-biofortification

References
[1] Stoltzfus R J. 2001. Iron-deficiency anemia: reexamining the nature and magnitude of the public health problem. Summary: implications for research and programs. Journal of Nutrition, 131: 697–700.
[2] Black RE. 2003. Zinc deficiency, infectious disease and mortality in the developing world. Journal of Nutrition, 133: 1485S–9S.
[3] Ma GS, Jin Y, Li Y P, Zhai F Y, Kok F, Jacobsen E and Yang X G. 2007. Iron and zinc deficiencies in China: what is a feasible and cost-effective strategy. Public Health Nutrition, 10: 1017-1023.
[4] White PJ, Broadley MR, Gregory PJ. 2012. Managing the Nutrition of Plants and People. Applied and Environmental Soil Science, 2012: 1-13.
[5] Frossard E, Bucher M, Machler F, Mozafar A, Hurrell R. 2000. Potential for increasing the content and bioavailability of Fe, Zn and Ca in plants for human nutrition. Journal of the Science of Food and Agriculture, 80 (7): 861-879.
[6] Welch R M, Graham R D. 2004. Breeding for micronutrients in staple food crops from a human nutrition perspective. Journal of Experimental Botany, 55: 353–364.
[7] Bouis HE, Hotz C, McClafferty B, Meenakshi JV, Pfeiffer WH (2011) Biofortification: A new tool to reduce micronutrient malnutrition. Food and Nutrition Bulletin, 32: S31-S40.
[8] Rengel Z, Batten G D, Crowley DE. 1999. Agronomic approaches for improving the micronutrient density in edible portions of crops. Field Crops Research, 60: 27-40.
[9] Cakmak I (2008) Enrichment of cereal grains with zinc: Agronomic or genetic biofortification? Plant and Soil, 302 (1-2): 1-17.
[10] Grotz N and Guerinot M L. 2006. Molecular aspects of Cu, Fe and Zn homeostasis in plants. Biochimica et Biophysica Acta, 1763: 595-608.
[11] Zhang W H, Zhou YC, Dibley K E, Tyerman S D, FurBank R T and Patrick J W. 2007. Nutrient loading of developing seeds. Functional Plant Biology, 34: 314-331.
[12] Waters BM, Sankaran RP (2011) Moving micronutrients from the soil to the seeds: Genes and physiological processes from a biofortification perspective. Plant Science, 180 (4): 562-574.
[13] Kim S A, Punshon T, Lanzirotti A, Li L T, Alonso J M. Echer J R. Kaplan J and Guerinot M L. 2006. Localization of iron in Arabidopsis seed requires the vacuolar membrane transporter VIT1. Science, 314: 1295-1299.
[14] Yi Y, Guerinot M L, Manthey J, Luster D, Crowley D E. 1994. Biochmistry of metal Micronutrients in the rhizosphere. CRC Press, Baca Raton, FL, pp259-307.
[15] Curie C, Briat J F. 2003. Iron transport and signaling in plants. Annual Review in Plant Biology, 54: 183-206.
[16] Bienfait H F. 1988. Mechanisms in Fe-efficiency reactions of higher plants. Journal Plant Nutrition, 11: 605-610.
[17] Colangelo E P, Guerinot M L. 2004. The essential bHLH protein FIT1 is requied for the iron deficiency response. Plant Cell, 16: 3400-3412.
[18] Schmidt W, Michalke W, Schikora A. 2003. Proton pumping by tomato roots. Effect of Fe deficiency and hormones on the activity and distribution of plasma membrane H+-ATPase in rhizodermal cells. Plant Cell and Environment, 26: 361-370.
[19] Robinson E L, Proctor C M, Connolly E L, Guerinot M L. 1999. A ferric chelate reductase for iron uptake from soils. Nature, 394: 694-697.
[20] Connolly E L, Campbell N, Grotz N, Prichard C L, Guerinot M L. 2003. Overexpressing of the FRO2 iron reductase confers tolerance to growth on low iron and uncovers post-transcriptional control. Plant Physiology, 133; 1102-1110.
[21] Mukherjee I, Campbell N H, Ash J S, Connolly E L. 2005. Expression profiling of the Arabidopsis ferric chelate reductase (FRO) gene family reveals differential regulation by iron and copper. Planta, 223: 1178-1190.
[22] Maser P, Thomie S, Schroeder J I, Ward J M, Hirschi K, Sze H, Talke I N, Amtann A, Matthuis F J M. Sanders D, Harper J F, Tchieu J, Gribskov M, Persans M W, Salt D E, Kim S A, Guerinot M L. 2001. Phylogenetic relationships within cation transporter family of Arabidopsis, Plant physiology, 126: 1646-1667.
[23] Henriques R, Jasik J, Klein M, martinoia E, Feller U, Schell J, Pais M S, Koncz C. 2002. Knock-out of rabidopsis metal transporter gene IRT1 results in iron deficiency accompanied by cell differentiation defects. Plant Molecular and Biology, 50: 587-597.
[24] Ishimaru Y, Suzuki M, Tsukamoto T, Suzuki K, Nakazono M, Kobayashi T, Wada Y, Watanabe S, Matsuhashi S, Takahashi M, Nakanishi H, Mori S, Nishizawa N K. 2006. Rice plants take up iron as a Fe3+-phtosiderphore and as Fe2+. Plant Journal, 2006: 335-346.
[25] Vert G, Grotz N, Dedaldechamp F, Gaymard F, Guerinot M L, Briat J F, Curie C. 2002. IRT1, an Arabidopsis transporter essential for iron uptake from the soil and plant growth. Plant Cell, 14: 1223-1233.
[26] Vert G, Briat J F, Curie C. 2001. Arabidopsis IRT2 gene encodes a root-periphery transporter. Plant Journal, 26: 181-189.
[27] Varotto C, Maiwald D, Pesaresi P, Jahns P, Francesco S, Leister D. 2002. The metal ion transporter IRT1 is necessary for iron homeostasis and efficient photosynthesis in Arabidopsis thaliana. Plant Journal, 31: 589-599.
[28] Curie C, Panaviene, Loulergue C, Dellaporta S L, Briat J F, Walker E L. 2001. Maize yellow stripe1 encodes a membrane protein directly involved in Fe (III) uptake. Nature, 409: 346-349.
[29] Briat J-F, Dubos C, Gaymard F (2015) Iron nutrition, biomass production, and plant product quality. Trends in Plant Science, 20 (1): 33-40.
[30] Terry N, Abadia J. 1986. Function of iron in chloroplasts. Journal of Plant nutrition, 9: 609-646.
[31] Thomine S, Lelivere L, Debarbieux E, Schroeder J I, Barbier-Bygoo h. 2003. AtNRAMP3, a multispecific vacuolar metal transporter involved in plant responses to iron efficiency. Plant Journal, 34: 685-695.
[32] Lanquar V, Lelievre F, Bolte S, Hames C, Alcon C, Neumann D, Vansuyt G, Curie C, Schroder A, Kramer U, Barbier-brygoo H, Thomine S. 2005. Mobilization of vacuolar iron by AtNRAMP3 and AtNRAMP4 is essential for seed germination on low iron. EMBO Journal, 24: 4041-4051.
[33] Didonato R J, Roberts L, Sanderson T, Eisley R, Walker E. 2004. Arabidopsis yellow stripe-lile2 (YSL2): a metal-regulated gene encoding a plasma membrane transporter of nicotianamine-metal complexes. Plant Journal, 39: 403-414.
[34] Le Jean M, Schikora A, Mari S, Briat J F, Curie C. 2005. A loss-function mutation in AtSYL1 reveals its role in iron and nicotianamine seed loading. Plant Journal, 44: 769-782.
[35] Koike S, Inoue H, Mizuno D, Takahashi M, Nakanishi H, Mori S, Nishizawa N. 2004. OsYSL2 is a rice metal-nicotianamine transporter that is regulated by iron and expressed in the phloem. Plant Journal, 39: 415-424.
[36] Yokosho K, Yamaji N, Fujii-Kashino M, Ma JF. 2016. Functional Analysis of a MATE Gene OsFRDL2 Revealed its Involvement in Al-Induced Secretion of Citrate, but a Lower Contribution to Al Tolerance in Rice. Plant and Cell Physiology, 57 (5): 976-985.
[37] Brown J C, Ambler J E. 1974. Iron-stress response in tomato (lycopersicon esculentum) 1. Sites of Fe reduction, absorption and transport. Physiologia Plantarum, 31: 221-224.
[38] Yuan Y X, Zhang J, Wang D W, Ling H Q. 2005. AtbHLH29 of Arabidopsis thaliana is a functional ortholog of tomato FER involved in controlling iron acquisition in strategy I plants. Cell Research, 15: 613-621.
[39] Kobayashi T, Ogo Y, Aung MS, Nozoye T, Itai RN, Nakanishi H, Yamakawa T, Nishizawa NK (2010) The spatial expression and regulation of transcription factors IDEF1 and IDEF2. Annals of Botany, 105 (7): 1109-1117.
[40] Kruger C, Berkowitz O, Stephan U W and Hell R. 2002. A metal-binding member of the late embryogenesis abundant protein family transports iron in the phloem of Ricinus communis L. Journal of Biological Chemistry, 277: 25062–25069.
[41] von Wiren N, Klair S, Bansal S, Briat J-F, Khodr H, Shioiri T, Leigh R A and Hider RC. 1999. Nicotianamine chelates both Fe (III) and Fe (II) Implications for metal transport in plants. Plant Physiology, 119: 1107–1114.
[42] Paul S, Datta SK, Datta K. 2015. miRNA regulation of nutrient homeostasis in plants. Frontiers in Plant Science, 6. doi: 10.3389/fpls.2015.00232.
[43] Ishimaru Y, Suzuki M, Kobayashi T, Takahashi M, Nakanishi H, Mori S and Nishizawa N K. 2005. OsZIP4, a novel zinc-regulated zinc transporter in rice. Journal of Experimental Botany, 56 (422): 3207-3214.
[44] Welch R M. 1995. Micronutrient nutrition of plants, critical review. Plant Science, 14: 9-82.
[45] Von Wiren N, Marschner H, Romheld V. 1996. Roots of iron-efficient maize also absorb phytosiderophore-chelated zinc. Plant Physiology, 111: 1119-1125.
[46] Graham R D. 1984. Breeding for nutritional characteristics in cereals. Advantage in Plant Nutrition, 1: 57-102.
[47] Schaaf G, Ludewig U, Erenoglu B E, Mori S, Kitahara T and von Wiren N. 2004. ZmYS1 functions as a proton-coupled symporter for phytosiderophore- and nicotianamine-chelated. Journal of Biological Chemistry, 279 (10): 9091-9096.
[48] Ramesh S A, Shin R, Eide D J, Schachtman D P. 2003. Differential metal selectivity and gene expression of two zinc transporters from rice. Plant Physiology, 133: 126-134.
[49] Lasswell J, Rogg L E, nelson D C, Rongey C, Bartel B. 2000. Cloning and characterization of IAR1, a gene required for auxin conjugate sensitivity in Arabidopsis. Plant Cell, 12: 2395-2408.
[50] Grotz N, Fox T, Connolly E L, Park W, Guerinot M L, Eide D. 1998. Identification of a family of zinc transporter genes from Arabidopsis that respond to zinc deficiency. PNAS, 95: 7220-7224.
[51] Olsen LI, Palmgren MG. 2014. Many rivers to cross: the journey of zinc from soil to seed. Frontiers in Plant Science, 5. doi: 10.3389/fpls.2014.00030.
[52] Kobae Y, Uemura T, Sato M H, Ohnishi M, Mimura T, Nakagawa T, Maeshima M. 2004. Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiology, 45: 1749-1758.
[53] Fujiwara T, Kawachi M, Sato Y, Mori H, Kutsuna N, Hasezawa S, Maeshima M. 2015. A high molecular mass zinc transporter MTP12 forms a functional heteromeric complex with MTP5 in the Golgi in Arabidopsis thaliana. FEBS Journal, 282 (10): 1965-1979.
[54] Williams L E, Mills R F. 2005. P (1B)-ATPase –An ancient family of transition metal umps with diverse functions in plants. Trends in plant science, 10: 491-502.
[55] Hussain D, Haydon M J, Wang Y, Wong E, sherson S M, Yong J, Camakaris J. harper J F. Cobbett C S. 2004. P-type ATPase heavy metal transporters with roles in essential zinc homeostasis in Arabidopsis. Plant Cell, 16: 1327-1339.
[56] Eren E, Arguello J M. 2004. Arabidopsis HMA2, a divalent heave metal-transporting P (IB)-type ATPase, is involved in cytoplasmic Zn2+ homeostasis. Plant Physiology, 136: 3712-3723.
[57] Verret F, Gravot A, Auroy P, leonhardt N, David P, Nussaume L, Vavasseur A, Richaud P. 2004. Overexpression of AtHMA4 enhances root-to-shoot translocation of zinc and cadmium and plant metal tolerance. FEBS Letter, 576: 306-312.
[58] Elbaz B, Shoshani-Knaani, David-Assael O, Mizrachy-Dagri T, Mizrahi K, Saul H, Brook E, Berezin I, Shaul O. 2006. High expression in leaves of the zinc hyperaccumulator Arabidopsis halleri of AhMHX, a homolog of an Arabidopsis thaliana vacuolar metal/proton exchanger. Plant Cell and Environment, 29: 1179-1190.
[59] White PJ, Broadley MR. 2009. Biofortification of crops with seven mineral elements often lacking in human diets - iron, zinc, copper, calcium, magnesium, selenium and iodine. New Phytologist, 182 (1): 49-84.
[60] Goto Fumiyukl, Yoshihara Toshihiro, Shigenoto Naokl, Tokl Seiichi, Takaiwa Fumio. 1999. Iron fortification of rice seed by the soybean ferritin gene. Nature Biotechnology, 17: 282-286.
[61] Ishimaru Y, Kim S, Tsukamoto T, Oki H, Kobayashi T, Watanabe S, Matsuhashi S, Takahashi M, Nakanishi H, Mori S, and Nishizawa N. 2007. Mutational reconstructed ferric chelate reductase confers enhanced tolerance in rice to iron deficiency in calcareous soil. PNAS, 104 (18): 7373-7378.
[62] Shi R, Zhang Y, Chen X, Sun Q, Zhang F, Römheld V, Zou C. 2010. Influence of long-term nitrogen fertilization on micronutrient density in grain of winter wheat (Triticum aestivum L.). Journal of Cereal Science, 51 (1): 165-170.
[63] Distelfeld Assaf, Cakmak Ismail, Peleg Zvi, Ozturk Levent, Yazici Atilla M, Budak Hikmet, Saranga Yehoshua and Fahima Tzion. 2007. Multiple QTL-effects of wheat Gpc-B1 locus on grain protein and micronutrient concentrations. Physiologia Plantarum, 129: 635-643.
[64] Ricroch AE, Henard-Damave M-C. 2016. Next biotech plants: new traits, crops, developers and technologies for addressing global challenges. Critical Reviews in Biotechnology, 36 (4): 675-690.
[65] Tilman D, Clark M. 2014. Global diets link environmental sustainability and human health. Nature, 515 (7528): 518.
[66] Welch R M, Graham R D. 1999. A new paradigm for world agriculture: meeting human needs productive, sustainable, nutritious. Field Crops Research, 60: 1-10.
[67] Joy EJM, Stein AJ, Young SD, Ander EL, Watts MJ, Broadley MR. 2015. Zinc-enriched fertilisers as a potential public health intervention in Africa. Plant and Soil, 389 (1-2): 1-24.
[68] Abadia J, Vazquez S, Rellan-Alvarez R, El-Jendoubi H, Abadia A, Alvarez-Fernandez A, Flor Lopez-Millan A. 2011. Towards a knowledge-based correction of iron chlorosis. Plant Physiology and Biochemistry, 49 (5): 471-482.
[69] Shenker M, Chen Y. 2005. Increasing iron availability to crops: Fertilizers, organo-fertilizers, and biological approaches. Soil Science and Plant Nutrition, 51 (1): 1-17.
[70] Poblaciones MJ, Rengel Z. 2016. Soil and foliar zinc biofortification in field pea (Pisum sativum L.): Grain accumulation and bioavailability in raw and cooked grains. Food Chemistry, 212: 427-433.
[71] Yilmaz A, Ekiz H, Torun B, Go ltekin I, Karanlyk S, Bagcy SA & Cakmak I. 1997. Effect of different zinc application methods on grain yield, and zinc concentrations in wheat grown on zinc-deficient calcareous soils in Central Anatolia. Journal of Plant Nutrition, 20: 461–471.
[72] Zou CQ, Zhang YQ, Rashid A, Ram H, Savasli E, Arisoy RZ, Ortiz-Monasterio I, Simunji S, Wang ZH, Sohu V, Hassan M, Kaya Y, Onder O, Lungu O, Mujahid MY, Joshi AK, Zelenskiy Y, Zhang FS, Cakmak I. 2012. Biofortification of wheat with zinc through zinc fertilization in seven countries. Plant and Soil, 361 (1-2): 119-130.
[73] Yin HJ, Gao XP, Stomph T, Li LJ, Zhang FS, Zou CQ. 2016. Zinc Concentration in Rice (Oryza sativa L.) Grains and Allocation in Plants as Affected by Different Zinc Fertilization Strategies. Communications in Soil Science and Plant Analysis, 47 (6): 761-768.
[74] Kutman UB, Yildiz B, Cakmak I. 2011. Effect of nitrogen on uptake, remobilization and partitioning of zinc and iron throughout the development of durum wheat. Plant and Soil, 342 (1-2): 149-164.
[75] Shahzad Z, Rouached H, Rakha A. 2014. Combating Mineral Malnutrition through Iron and Zinc Biofortification of Cereals. Comprehensive Reviews in Food Science and Food Safety, 13 (3): 329-346.
[76] Zhang YQ, Deng Y, Chen RY, Cui ZL, Chen XP, Yost R, Zhang FS, Zou CQ. 2012. The reduction in zinc concentration of wheat grain upon increased phosphorus-fertilization and its mitigation by foliar zinc application. Plant and Soil, 361 (1-2): 143-152.
[77] Zuo Y M, Zhang F S, Li X L and Cao Y P. 2000. Studies on the improvement in iron nutrition of peanut by intercropping with maize on a calcareous soil. Plant Soil, 220: 13-25.
[78] Harris D, Rashid A, Miraj G, Arif M, Shah H. 2007. “On-farm” seed priming with zinc sulphate solution−A cost-effective way to increase the maize yields of resource-poor farmers. Field Crops Research, 102: 119-127.
[79] Wang Y-H, Zou C-Q, Mirza Z, Li H, Zhang Z-Z, Li D-P, Xu C-L, Zhou X-B, Shi X-J, Xie D-T, He X-H, Zhang Y-Q. 2016. Cost of agronomic biofortification of wheat with zinc in China. Agronomy for Sustainable Development, 36 (44): 1-7.
Author Information
  • College of Resources and Environment, Southwest University, Chongqing, China

  • College of Resources and Environment, Southwest University, Chongqing, China

  • College of Resources and Environment, Southwest University, Chongqing, China

  • College of Resources and Environment, Southwest University, Chongqing, China

  • College of Resources and Environment, Southwest University, Chongqing, China; National Monitoring Stations of Soil Fertility and Fertilizer Efficiency on Purple Soil, Chongqing, China

  • College of Resources and Environment, Southwest University, Chongqing, China; National Monitoring Stations of Soil Fertility and Fertilizer Efficiency on Purple Soil, Chongqing, China

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    Jing Liu, Mingyue Yang, Hong Li, Danping Li, Xiaojun Shi, et al. (2017). Genetic Processes of Iron and Zinc Accumulation in Edible Portion of Crops and Their Agro-Biofortification: A Review. American Journal of Agriculture and Forestry, 5(3), 65-72. https://doi.org/10.11648/j.ajaf.20170503.15

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    Jing Liu; Mingyue Yang; Hong Li; Danping Li; Xiaojun Shi, et al. Genetic Processes of Iron and Zinc Accumulation in Edible Portion of Crops and Their Agro-Biofortification: A Review. Am. J. Agric. For. 2017, 5(3), 65-72. doi: 10.11648/j.ajaf.20170503.15

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    AMA Style

    Jing Liu, Mingyue Yang, Hong Li, Danping Li, Xiaojun Shi, et al. Genetic Processes of Iron and Zinc Accumulation in Edible Portion of Crops and Their Agro-Biofortification: A Review. Am J Agric For. 2017;5(3):65-72. doi: 10.11648/j.ajaf.20170503.15

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  • @article{10.11648/j.ajaf.20170503.15,
      author = {Jing Liu and Mingyue Yang and Hong Li and Danping Li and Xiaojun Shi and Yueqiang Zhang},
      title = {Genetic Processes of Iron and Zinc Accumulation in Edible Portion of Crops and Their Agro-Biofortification: A Review},
      journal = {American Journal of Agriculture and Forestry},
      volume = {5},
      number = {3},
      pages = {65-72},
      doi = {10.11648/j.ajaf.20170503.15},
      url = {https://doi.org/10.11648/j.ajaf.20170503.15},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajaf.20170503.15},
      abstract = {Iron (Fe) and zinc (Zn) are essential micronutrient for both human and plants, but Fe and Zn deficiency is prevalent in the world especially developing countries including India and China. Biofortification is considered the most promising approach to alleviate Fe and Zn malnutrition. Thus this study was mainly conducted to review the recent progresses on the strategies of the processes affecting Fe and Zn accumulation in edible portion of crops at genetic and physiological levels. While agricultural approaches are useful to gain Fe and Zn enriched cereals, therefore agro-biofortification of Fe and Zn by agricultural approaches was also reviewed for possible solution in intensive agriculture system.},
     year = {2017}
    }
    

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    AU  - Jing Liu
    AU  - Mingyue Yang
    AU  - Hong Li
    AU  - Danping Li
    AU  - Xiaojun Shi
    AU  - Yueqiang Zhang
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    JF  - American Journal of Agriculture and Forestry
    JO  - American Journal of Agriculture and Forestry
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    SN  - 2330-8591
    UR  - https://doi.org/10.11648/j.ajaf.20170503.15
    AB  - Iron (Fe) and zinc (Zn) are essential micronutrient for both human and plants, but Fe and Zn deficiency is prevalent in the world especially developing countries including India and China. Biofortification is considered the most promising approach to alleviate Fe and Zn malnutrition. Thus this study was mainly conducted to review the recent progresses on the strategies of the processes affecting Fe and Zn accumulation in edible portion of crops at genetic and physiological levels. While agricultural approaches are useful to gain Fe and Zn enriched cereals, therefore agro-biofortification of Fe and Zn by agricultural approaches was also reviewed for possible solution in intensive agriculture system.
    VL  - 5
    IS  - 3
    ER  - 

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