Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production

Nguyen Thi Hoa; Tang Thi Chinh; Dang Thi Mai Anh; Ngo Dinh Binh; Le Thi Minh Thanh

doi:doi:10.11648/j.ajaf.20140205.12

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Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production

Nguyen Thi Hoa, Tang Thi Chinh, Dang Thi Mai Anh, Ngo Dinh Binh, Le Thi Minh Thanh

Published in American Journal of Agriculture and Forestry (Volume 2, Issue 5)

Received: 11 August 2014 Accepted: 28 August 2014 Published: 10 September 2014

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Abstract

Optimization of medium compositions from dewatered wastewater sludge of beer manufactory for delta endotoxin production by Bacillus thuringiensis was investigated in flask fermentation. Its composition consisted of wastewater sludge hydrolysis broth with several agricultural byproducts (rice bran, soybean meal, corn flour) and mineral salts (MgSO4.7H2O; K2HPO4; KH2PO4; MnSO4; CaCl2; NaCl). Rice bran and MgSO4.7H2O, CaCl2 were all found to have a significant influence on delta endotoxin production. The optimal concentration of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for delta endotoxin production were determined as follows: WWS (20 g/l, dry weight), CaCl2 (0.3 g/l), MgSO4.7H2O (0.32 g/l), and rice bran (5.8 g/l). Using this optimized fermentation media, the yield of delta endotoxin was increased by 34% to 565 mg/l compared with unoptimal medium. Viable cell and spore counts obtained in optimum fermented broth were 1.25x109 CFU/ml, 1.07x109 CFU/ml, respectively. The LC50 value of Bacillus thuringiensis serovar isralensis against Culex quinquefasciatus was 0.056 mg/l and there was no significant difference between LC50 value of bacterium grown in the medium.

Published in	American Journal of Agriculture and Forestry (Volume 2, Issue 5)
DOI	10.11648/j.ajaf.20140205.12
Page(s)	219-225
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bacillus thuringiensis, Delta endotoxin, Dewatered Wastewater Sludge, Bio-Pesticide

References

[1]	A., Yezza, R.D.Tyagi, J.R.Valero, and R.Y.Surampalli (2005) Bioconversion of industrial wastewater and wastewater sludge in Bacillus Thuringiensis based biopesticides in pilot fermentor, Bioresource Technology. 97, pp. 1850-1857.
[2]	Apha, Awwa, and Wpcf (1998) Standard methods for examination of water and wastewaters (sections 2541B-E, 5310B, 4500¬NH3 H, 4500-N B and 4500-P H), 20th edn. In: Clesceri LS, Greenberg AE, Eaton AD (eds)DC American Public Health Association, Washington.
[3]	Binh, Ngo Dinh, Tuan Nguyen Dinh, Ha Trinh Thu, Duong Pham Thuy, Huong Pham Minh, and Luy Nguyen Thi (2009) Production and Evaluation of Mosquitocidal Efficacy of Bacillus thuringensis subsp. israelensis Based Formulations in Viet Nam, Proceedings of the 2nd International Conference of Integrated Pest Management in Asia and Africa. Science and Technics Publishing House. 2, pp. 279-283.
[4]	Binh, Ngo Dinh, Thanh Le Thi Minh, Ha Trinh Thi Thu, Thuy Pham Kieu, Nhung Le Thi Hong, and Tien Dang Van (2011) 35 years – Research and Development of Bacillus thuringiensis Biopesticides in Vietnam, Proceedings of the 4th International Conference of Integrated Pest Management in Asia and Africa. Bangladesh Agricultural University, pp. 87-91.
[5]	Box, Gep and Wilson Kb. (1951) On the experimental attainment of optimum conditions, J. Roy. Stat. Soc., pp. 13-145.
[6]	Bradford, Mm (1976) A rapid and sensitive method for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding, Analytical Biochem. 72, pp. 248-254.
[7]	Braun, S. (2000) Bioassays of Bacillus thuringiensis 1D. Production of Bacillus thuringiensis insecticides for experimental uses, In: Navon, A., Ascher, K.R.S (Eds.), Bioassays of Entomopathogenic Microbes and Nematodes. CAB International, pp. 49-72.
[8]	Gao, Huiju , Xiang Chu, Yanwen Wang, Fei Zhou, Kai Zhao, Zhimei Mu, and Qingxin Liu (2013) Media Optimization for Laccase Production by Trichoderma harzianum ZF-2 Using Response Surface Methodology, J. Microbiol. Biotechnol. 23(12), pp. 1757-1764.
[9]	Içgen, Y., B. Içgen, and Ozcengiz (2002) Regulation of crystal protein biosynthesis byBacillus thuringiensis: I. EVects of mineral elements and pH, Research in Microbiology 153(9), pp. 599-603.
[10]	Jaekoo, Lee, Inkyung Park, Sangbuem Cho, Sooki Kim, Yunjaie Choi, and Jaiesoon Cho (2013) Optimization of medium composition for α-galactosidase production by Antarctic bacterial isolate, Bacillus sp. LX-1 using response surface methodology, African Journal of Microbiology Research. 7(27), pp. 3494-3500.
[11]	Khanh, Dang Vu, R.D. Tyagi, S.K. Brar, J.R. Valero, and R.Y. Surampali (2009) Starch industry wastewater for production of biopesticides – ramifications of solids concentrations, Environmental Technology. 3(4), pp. 393-405.
[12]	Khanh, Vu Dang, Rajeshwar Dayal Tyagi, José R. Valéro, and Rao Y. Surampalli (2009) Bath and fed-bath fermentation of Bacillus thuringiensis using starch industry wastewater as fermentation substrate, Bioprocess and Biosystems Engineering. 33, pp. 691-700.
[13]	Lachhab, K., Tyagi R.D., and Valero J.R (2001) Production of Bacillus thuringiensis biopesticides using wastewater sludge as a raw material: eVect of inoculum and sludge solids concentration, Process Biochemistry. 37(2), pp. 197-208.
[14]	Li, Y., Liu Z., Zhao H., Xu Y., and Cui F. (2007) Statistical optimization of xylanase production from new isolated Penicillium oxalicum ZH-30 in submerged fermentation, Biochem. Eng. J. 34, pp. 82-86.
[15]	Linna, Du, Jia Song, Hongbin Wang, Peng Li, Zhongzhou Yang, Lingjun Meng, Fanqing Meng, Jiahui Lu, and Lirong Teng (2012) Optimization of the fermentation medium for Paecilomyces tenuipes N45 using statistical approach, African Journal of Microbiology Research. 6(32), pp. 6130-6141.
[16]	Ozkan, M., F.B. Dilek, U. Yetis, and G. Ozcenzig (2003) Nutritional and cultural parameters in Xuencing antidipteran delta-endotoxin production, Research in Microbiology. 154, pp. 49-53.
[17]	Songqing, Wu, Yanjiao Lan, Dongmei Huang, Yan Peng, Zhipeng Huang, Lei Xu, Ivan Gelbic, Rebeca Carballar Lejarazu, Xiong Guan, Lingling Zhang, and Shuangquan Zou (2014) Use of Spent Mushroom Substrate for Production of Bacillus thuringiensis by Solid-State Fermentation, Journal of Economic Entomology. 107(1), pp. 137-143.
[18]	Stanbury, P.F., A. Whitaker, and S.J. Hall (1995) Principles of Fermentation Technology, Second ed. Elsevier Science Ltd., New York.
[19]	Subbiah, Poopathi and B. Archana (2012) Optimization of medium composition of mosquitocidal toxins from Bacillus thuringiensis Subsp. Isrealensis, Indian Journal of Experimental Biology. 50, pp. 65-71.
[20]	Vidyarthi, A.S., Tyagi R.D., Valéro J.R., and Surampalli R.Y. (2002) Studies on the production of B. thuringiensis based biopesticides using wastewater sludge as raw material, Water Research. 36(19), pp. 4850-4860.
[21]	Vlyssides, A.G. and P.K. Karlis (2004) Thermal-alkaline solubilization of waste activated sludge as a pre-treatment stage for anaerobic digestion, Bioresource Technology. 91, pp. 201-206.
[22]	Zouari, N. and Jaoua S. (1999) The effect of complex carbon and nitrogen, salt, Tween-80 and acetate on delta-endotoxin production by a Bacillus thuringiensis subsp. kurstaki, J Ind Microbiol Biotechnol. 23, pp. 497-502.
[23]	Zouari, N. and Jaoua S. (1999) Production and characterization of metalloproteases synthesized concomitantly with delta-endotoxin by Bacillus thuringiensis subsp. kurstaki strain grown on gruelbased media, Enzyme Microb Technol. 25, pp. 364-371.
[24]	Zouari, Z., Ben Sik Ali S., and Jaoua S. (2002) Production of delta-endotoxin by several Bacillus thuringiensis strains exhibiting various entomocidal activities towards lepidoptera and diptera in gruel and fish-meal media, Enzyme Microb Technol. 31, pp. 411-418.

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Nguyen Thi Hoa, Tang Thi Chinh, Dang Thi Mai Anh, Ngo Dinh Binh, Le Thi Minh Thanh. (2014). Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production. American Journal of Agriculture and Forestry, 2(5), 219-225. https://doi.org/10.11648/j.ajaf.20140205.12

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ACS Style

Nguyen Thi Hoa; Tang Thi Chinh; Dang Thi Mai Anh; Ngo Dinh Binh; Le Thi Minh Thanh. Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production. Am. J. Agric. For. 2014, 2(5), 219-225. doi: 10.11648/j.ajaf.20140205.12

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AMA Style

Nguyen Thi Hoa, Tang Thi Chinh, Dang Thi Mai Anh, Ngo Dinh Binh, Le Thi Minh Thanh. Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production. Am J Agric For. 2014;2(5):219-225. doi: 10.11648/j.ajaf.20140205.12

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@article{10.11648/j.ajaf.20140205.12,
  author = {Nguyen Thi Hoa and Tang Thi Chinh and Dang Thi Mai Anh and Ngo Dinh Binh and Le Thi Minh Thanh},
  title = {Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production},
  journal = {American Journal of Agriculture and Forestry},
  volume = {2},
  number = {5},
  pages = {219-225},
  doi = {10.11648/j.ajaf.20140205.12},
  url = {https://doi.org/10.11648/j.ajaf.20140205.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20140205.12},
  abstract = {Optimization of medium compositions from dewatered wastewater sludge of beer manufactory for delta endotoxin production by Bacillus thuringiensis was investigated in flask fermentation. Its composition consisted of wastewater sludge hydrolysis broth with several agricultural byproducts (rice bran, soybean meal, corn flour) and mineral salts (MgSO4.7H2O; K2HPO4; KH2PO4; MnSO4; CaCl2; NaCl). Rice bran and MgSO4.7H2O, CaCl2 were all found to have a significant influence on delta endotoxin production. The optimal concentration of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for delta endotoxin production were determined as follows: WWS (20 g/l, dry weight), CaCl2 (0.3 g/l), MgSO4.7H2O (0.32 g/l), and rice bran (5.8 g/l). Using this optimized fermentation media, the yield of delta endotoxin was increased by 34% to 565 mg/l compared with unoptimal medium. Viable cell and spore counts obtained in optimum fermented broth were 1.25x109 CFU/ml, 1.07x109 CFU/ml, respectively. The LC50 value of Bacillus thuringiensis serovar isralensis against Culex quinquefasciatus was 0.056 mg/l and there was no significant difference between LC50 value of bacterium grown in the medium.},
 year = {2014}
}

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TY  - JOUR
T1  - Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production
AU  - Nguyen Thi Hoa
AU  - Tang Thi Chinh
AU  - Dang Thi Mai Anh
AU  - Ngo Dinh Binh
AU  - Le Thi Minh Thanh
Y1  - 2014/09/10
PY  - 2014
N1  - https://doi.org/10.11648/j.ajaf.20140205.12
DO  - 10.11648/j.ajaf.20140205.12
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 219
EP  - 225
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20140205.12
AB  - Optimization of medium compositions from dewatered wastewater sludge of beer manufactory for delta endotoxin production by Bacillus thuringiensis was investigated in flask fermentation. Its composition consisted of wastewater sludge hydrolysis broth with several agricultural byproducts (rice bran, soybean meal, corn flour) and mineral salts (MgSO4.7H2O; K2HPO4; KH2PO4; MnSO4; CaCl2; NaCl). Rice bran and MgSO4.7H2O, CaCl2 were all found to have a significant influence on delta endotoxin production. The optimal concentration of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for delta endotoxin production were determined as follows: WWS (20 g/l, dry weight), CaCl2 (0.3 g/l), MgSO4.7H2O (0.32 g/l), and rice bran (5.8 g/l). Using this optimized fermentation media, the yield of delta endotoxin was increased by 34% to 565 mg/l compared with unoptimal medium. Viable cell and spore counts obtained in optimum fermented broth were 1.25x109 CFU/ml, 1.07x109 CFU/ml, respectively. The LC50 value of Bacillus thuringiensis serovar isralensis against Culex quinquefasciatus was 0.056 mg/l and there was no significant difference between LC50 value of bacterium grown in the medium.
VL  - 2
IS  - 5
ER  -

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Author Information

Nguyen Thi Hoa

Dept of Environmental Microbiology, Institute of Environmental Technology, Vietnamese Academy of Science and Technology, Hanoi, Vietnam
Tang Thi Chinh

Dept of Environmental Microbiology, Institute of Environmental Technology, Vietnamese Academy of Science and Technology, Hanoi, Vietnam
Dang Thi Mai Anh

Dept of Environmental Microbiology, Institute of Environmental Technology, Vietnamese Academy of Science and Technology, Hanoi, Vietnam
Ngo Dinh Binh

Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Le Thi Minh Thanh

Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam

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Nguyen Thi Hoa, Tang Thi Chinh, Dang Thi Mai Anh, Ngo Dinh Binh, Le Thi Minh Thanh. (2014). Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production. American Journal of Agriculture and Forestry, 2(5), 219-225. https://doi.org/10.11648/j.ajaf.20140205.12

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Nguyen Thi Hoa; Tang Thi Chinh; Dang Thi Mai Anh; Ngo Dinh Binh; Le Thi Minh Thanh. Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production. Am. J. Agric. For. 2014, 2(5), 219-225. doi: 10.11648/j.ajaf.20140205.12

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AMA Style

Nguyen Thi Hoa, Tang Thi Chinh, Dang Thi Mai Anh, Ngo Dinh Binh, Le Thi Minh Thanh. Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production. Am J Agric For. 2014;2(5):219-225. doi: 10.11648/j.ajaf.20140205.12

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@article{10.11648/j.ajaf.20140205.12,
  author = {Nguyen Thi Hoa and Tang Thi Chinh and Dang Thi Mai Anh and Ngo Dinh Binh and Le Thi Minh Thanh},
  title = {Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production},
  journal = {American Journal of Agriculture and Forestry},
  volume = {2},
  number = {5},
  pages = {219-225},
  doi = {10.11648/j.ajaf.20140205.12},
  url = {https://doi.org/10.11648/j.ajaf.20140205.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20140205.12},
  abstract = {Optimization of medium compositions from dewatered wastewater sludge of beer manufactory for delta endotoxin production by Bacillus thuringiensis was investigated in flask fermentation. Its composition consisted of wastewater sludge hydrolysis broth with several agricultural byproducts (rice bran, soybean meal, corn flour) and mineral salts (MgSO4.7H2O; K2HPO4; KH2PO4; MnSO4; CaCl2; NaCl). Rice bran and MgSO4.7H2O, CaCl2 were all found to have a significant influence on delta endotoxin production. The optimal concentration of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for delta endotoxin production were determined as follows: WWS (20 g/l, dry weight), CaCl2 (0.3 g/l), MgSO4.7H2O (0.32 g/l), and rice bran (5.8 g/l). Using this optimized fermentation media, the yield of delta endotoxin was increased by 34% to 565 mg/l compared with unoptimal medium. Viable cell and spore counts obtained in optimum fermented broth were 1.25x109 CFU/ml, 1.07x109 CFU/ml, respectively. The LC50 value of Bacillus thuringiensis serovar isralensis against Culex quinquefasciatus was 0.056 mg/l and there was no significant difference between LC50 value of bacterium grown in the medium.},
 year = {2014}
}

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TY  - JOUR
T1  - Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production
AU  - Nguyen Thi Hoa
AU  - Tang Thi Chinh
AU  - Dang Thi Mai Anh
AU  - Ngo Dinh Binh
AU  - Le Thi Minh Thanh
Y1  - 2014/09/10
PY  - 2014
N1  - https://doi.org/10.11648/j.ajaf.20140205.12
DO  - 10.11648/j.ajaf.20140205.12
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 219
EP  - 225
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20140205.12
AB  - Optimization of medium compositions from dewatered wastewater sludge of beer manufactory for delta endotoxin production by Bacillus thuringiensis was investigated in flask fermentation. Its composition consisted of wastewater sludge hydrolysis broth with several agricultural byproducts (rice bran, soybean meal, corn flour) and mineral salts (MgSO4.7H2O; K2HPO4; KH2PO4; MnSO4; CaCl2; NaCl). Rice bran and MgSO4.7H2O, CaCl2 were all found to have a significant influence on delta endotoxin production. The optimal concentration of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for delta endotoxin production were determined as follows: WWS (20 g/l, dry weight), CaCl2 (0.3 g/l), MgSO4.7H2O (0.32 g/l), and rice bran (5.8 g/l). Using this optimized fermentation media, the yield of delta endotoxin was increased by 34% to 565 mg/l compared with unoptimal medium. Viable cell and spore counts obtained in optimum fermented broth were 1.25x109 CFU/ml, 1.07x109 CFU/ml, respectively. The LC50 value of Bacillus thuringiensis serovar isralensis against Culex quinquefasciatus was 0.056 mg/l and there was no significant difference between LC50 value of bacterium grown in the medium.
VL  - 2
IS  - 5
ER  -

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