Animal and Veterinary Sciences
Volume 2, Issue 6, November 2014, Pages: 208-212
Received: Dec. 3, 2014;
Accepted: Dec. 11, 2014;
Published: Dec. 19, 2014
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Rasha S. Ahmed, Department of Anatomy, Faculty of Veterinary Medicine, P.O. Box 32, University of Khartoum, Sudan, Cell Phone 00249916289454
Holger Martens, Institut of Physiology, Faculty of Veterinary Medicine (WE02), Oertzenweg 19b, Hs. 11 Raum 1.49, Haus B (rechts) 1. Stock 14163 Berlin
Christoph Muelling, Institut of Anatomy, Faculty of Veterinary Medicine, An den Tierkliniken 43,D-04103 Leipzig, Germany
The objective of this study was to characterize the immunohistochemical localization of Na+/H+ exchanger isoforms (NHE1 and NHE3) in the rumen epithelium of Sheep after changing the diet from hay (ad libitum) to a mixed hay/concentrate diet. A total of 24 sheep were fed mixed hay/concentrate for different periods ranging from 0 weeks (control; hay ad libitum) to 12 weeks (1-1.5 kg hay plus 780 g concentrate per day in two equal portions). NHE3-immunostaining was found to be more intense at both stratum granulosum (deep layer) and stratum spinosum (superficial layer), with decreasing intensity through stratum spinosum (deep or suprabasal layer) and stratum basal. Stratum corneum was negative. Distribution of NHE3 isoform was different within the different strata. In stratum granulosum and stratum spinosum (superficial layer), NHE3 isoform was distributed predominant at the apical surface /membrane of the cells. Meanwhile, in both stratum spinosum (deep layer) and stratum basale, intracellular NHE3 isoform was predominantly. The degree of antibody reaction was weak in hay-fed sheep and in all concentrate-fed groups, except in 2 and 4 weeks concentrate-fed groups, in which the degree of the antibody reaction was moderate and strong, respectively. NHE1 isoform was not detected in the sheep-rumen epithelium.
Rasha S. Ahmed,
Diet-Dependent Rumen Epithelial NHE1 and NHE3 Expression in Sheep, Animal and Veterinary Sciences.
Vol. 2, No. 6,
2014, pp. 208-212.
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