Animal and Veterinary Sciences

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Detection of Plasmid in Listeria monocytogenes Isolated from Fresh Raw Milk in Nigeria

Received: 02 October 2014    Accepted: 15 October 2014    Published: 30 October 2014
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Abstract

Aims: To determine the presence of plasmids in Listeria monocytogenes strains isolated from fresh raw milk from healthy cattle in Nigeria and also determine its role in antibiotics resistance since this organism is a food borne pathogen of public health importance. Methods and results: Isolation of Listeria monocytogenes was done using selective enrichment and isolation protocol, as recommended by United States Department of Agriculture (USDA) and Association of Analytical Chemists/ International Dairy Federation (AOAC/ IDF). Identification was by phenotypic characterization and confirmed using β-haemolytic activity, phosphatidyl inositol phospholipase-C (PIPLC) assay and polymerase chain reaction (PCR) . Strains identified as Listeria monocytogenes from the six zones were determined and results expressed as rate of prevalence in graph. Twenty-three strains of Listeria monocytogenes isolated from 305 samples of fresh raw milk were screened for the presence of plasmid DNA using the method of Ehrenfeld and Clewel and agarose gel electrophoresis. One isolate was found to harbour one plasmid with molecular weight of 14.62kb. The plasmid was cured using different concentrations of acridine orange. Antibiotic sensitivity test was carried out on both the wild strain (with plasmid) and cured strain using disc diffusion method of Bauer-Kirby and it was found that the wild strain was resistant to seven of the twelve antibiotics used while the cured strain was resistant to only one. Conclusions: There is a possibility of the presence of strains of Listeria monocytogenes that can harbour plasmids. This could confer antibiotic resistance to the strains and thus make antibiotic therapy unsuccessful. The increase in the number of antibiotics which the cured strain of Listeria monocytogenes was susceptible to in this study showed that plasmid may play a significant role in antibiotics resistance. The Fulani herdsmen who take fresh raw milk without boiling or pasteurization may serve as carriers of resistant strains of Listeria monocytogenes and help in its spread in the environment through faecal pollution.

DOI 10.11648/j.avs.20140206.12
Published in Animal and Veterinary Sciences (Volume 2, Issue 6, November 2014)
Page(s) 169-174
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Fresh Raw Milk, Listeria monocytogenes, Plasmid, Antibiotic Resistance

References
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Author Information
  • National Veterinary Research Institute, Vom, Plateau State Nigeria

  • Department of Microbiology, Faculty of Science, University of Lagos, Lagos, Nigeria

  • Department of Microbiology, Faculty of Science, University of Lagos, Lagos, Nigeria

  • Molecular Biology and Biotechnology Division, Nigerian Institute for Medical Research, Yaba, Lagos, Nigeria

  • Molecular Biology and Biotechnology Division, Nigerian Institute for Medical Research, Yaba, Lagos, Nigeria

  • National Veterinary Research Institute, Vom, Plateau State Nigeria

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    Enurah Leonard Uwanibe, Aboaba Olusimbo Olugbo, Nwachukwu Simon Cyril Ugwumba, Smith Stella Ifeanyi, Fowora Adenike, et al. (2014). Detection of Plasmid in Listeria monocytogenes Isolated from Fresh Raw Milk in Nigeria. Animal and Veterinary Sciences, 2(6), 169-174. https://doi.org/10.11648/j.avs.20140206.12

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    Enurah Leonard Uwanibe; Aboaba Olusimbo Olugbo; Nwachukwu Simon Cyril Ugwumba; Smith Stella Ifeanyi; Fowora Adenike, et al. Detection of Plasmid in Listeria monocytogenes Isolated from Fresh Raw Milk in Nigeria. Anim. Vet. Sci. 2014, 2(6), 169-174. doi: 10.11648/j.avs.20140206.12

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    AMA Style

    Enurah Leonard Uwanibe, Aboaba Olusimbo Olugbo, Nwachukwu Simon Cyril Ugwumba, Smith Stella Ifeanyi, Fowora Adenike, et al. Detection of Plasmid in Listeria monocytogenes Isolated from Fresh Raw Milk in Nigeria. Anim Vet Sci. 2014;2(6):169-174. doi: 10.11648/j.avs.20140206.12

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  • @article{10.11648/j.avs.20140206.12,
      author = {Enurah Leonard Uwanibe and Aboaba Olusimbo Olugbo and Nwachukwu Simon Cyril Ugwumba and Smith Stella Ifeanyi and Fowora Adenike and Nwosuh Chika Ignatius},
      title = {Detection of Plasmid in Listeria monocytogenes Isolated from Fresh Raw Milk in Nigeria},
      journal = {Animal and Veterinary Sciences},
      volume = {2},
      number = {6},
      pages = {169-174},
      doi = {10.11648/j.avs.20140206.12},
      url = {https://doi.org/10.11648/j.avs.20140206.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.avs.20140206.12},
      abstract = {Aims: To determine the presence of plasmids in Listeria monocytogenes strains isolated from fresh raw milk from healthy cattle in Nigeria and also determine its role in antibiotics resistance since this organism is a food borne pathogen of public health importance. Methods and results: Isolation of Listeria monocytogenes was done using selective enrichment and isolation protocol, as recommended by United States Department of Agriculture (USDA) and Association of Analytical Chemists/ International Dairy Federation (AOAC/ IDF). Identification was by phenotypic characterization and confirmed using β-haemolytic activity, phosphatidyl inositol phospholipase-C (PIPLC) assay and polymerase chain reaction (PCR) . Strains identified as Listeria monocytogenes from the six zones were determined and results expressed as rate of prevalence in graph. Twenty-three strains of Listeria monocytogenes isolated from 305 samples of fresh raw milk were screened for the presence of plasmid DNA using the method of Ehrenfeld and Clewel and agarose gel electrophoresis. One isolate was found to harbour one plasmid with molecular weight of 14.62kb. The plasmid was cured using different concentrations of acridine orange. Antibiotic sensitivity test was carried out on both the wild strain (with plasmid) and cured strain using disc diffusion method of Bauer-Kirby and it was found that the wild strain was resistant to seven of the twelve antibiotics used while the cured strain was resistant to only one. Conclusions: There is a possibility of the presence of strains of Listeria monocytogenes that can harbour plasmids. This could confer antibiotic resistance to the strains and thus make antibiotic therapy unsuccessful. The increase in the number of antibiotics which the cured strain of Listeria monocytogenes was susceptible to in this study showed that plasmid may play a significant role in antibiotics resistance. The Fulani herdsmen who take fresh raw milk without boiling or pasteurization may serve as carriers of resistant strains of Listeria monocytogenes and help in its spread in the environment through faecal pollution.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Detection of Plasmid in Listeria monocytogenes Isolated from Fresh Raw Milk in Nigeria
    AU  - Enurah Leonard Uwanibe
    AU  - Aboaba Olusimbo Olugbo
    AU  - Nwachukwu Simon Cyril Ugwumba
    AU  - Smith Stella Ifeanyi
    AU  - Fowora Adenike
    AU  - Nwosuh Chika Ignatius
    Y1  - 2014/10/30
    PY  - 2014
    N1  - https://doi.org/10.11648/j.avs.20140206.12
    DO  - 10.11648/j.avs.20140206.12
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
    SP  - 169
    EP  - 174
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20140206.12
    AB  - Aims: To determine the presence of plasmids in Listeria monocytogenes strains isolated from fresh raw milk from healthy cattle in Nigeria and also determine its role in antibiotics resistance since this organism is a food borne pathogen of public health importance. Methods and results: Isolation of Listeria monocytogenes was done using selective enrichment and isolation protocol, as recommended by United States Department of Agriculture (USDA) and Association of Analytical Chemists/ International Dairy Federation (AOAC/ IDF). Identification was by phenotypic characterization and confirmed using β-haemolytic activity, phosphatidyl inositol phospholipase-C (PIPLC) assay and polymerase chain reaction (PCR) . Strains identified as Listeria monocytogenes from the six zones were determined and results expressed as rate of prevalence in graph. Twenty-three strains of Listeria monocytogenes isolated from 305 samples of fresh raw milk were screened for the presence of plasmid DNA using the method of Ehrenfeld and Clewel and agarose gel electrophoresis. One isolate was found to harbour one plasmid with molecular weight of 14.62kb. The plasmid was cured using different concentrations of acridine orange. Antibiotic sensitivity test was carried out on both the wild strain (with plasmid) and cured strain using disc diffusion method of Bauer-Kirby and it was found that the wild strain was resistant to seven of the twelve antibiotics used while the cured strain was resistant to only one. Conclusions: There is a possibility of the presence of strains of Listeria monocytogenes that can harbour plasmids. This could confer antibiotic resistance to the strains and thus make antibiotic therapy unsuccessful. The increase in the number of antibiotics which the cured strain of Listeria monocytogenes was susceptible to in this study showed that plasmid may play a significant role in antibiotics resistance. The Fulani herdsmen who take fresh raw milk without boiling or pasteurization may serve as carriers of resistant strains of Listeria monocytogenes and help in its spread in the environment through faecal pollution.
    VL  - 2
    IS  - 6
    ER  - 

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