| Peer-Reviewed

Protein and Electrophoretic Analysis of Edible Muscle of Commercially Important Crustaceans and Mollusks Species from Egyptian and Saudi Arabia Costs

Received: 20 May 2014    Accepted: 12 June 2014    Published: 20 July 2014
Views:       Downloads:
Abstract

Crustaceans and mollusks are important components of the aquatic fauna. Furthermore, crustaceans and mollusks consumption have been increasing worldwide during the past decades. So, therefore, the present study was investigated the variation between total protein content and protein electrophoretic pattern in edible muscle of commercially important crustaceans (Erugosquilla massavensis, Peaneus semisulcatus, Metapenaeus monoceros and Portunus pelagicus) and mollusks (Sepia spp., Cardium edule) as an attempt to rank them as an alternative rich animal protein source for human. The recorded data declare that the highest protein percentage was observed in males E. massavensis followed by Sepia spp then P. pelagicus and shrimps. On the other hand, females E. massavensis and bivalve mollusks (C. edule) had less total protein contents in their muscles, additionally, the recorded data showed a variation in band numbers and estimated molecular weight of muscle protein between sexes of each species and among the studied species. The results of the present work clearly indicate that there are differences in the protein structure of the muscles in different species of crustaceans and mollusks with reference to the protein fractions and their molecular weights.

Published in Animal and Veterinary Sciences (Volume 2, Issue 4)
DOI 10.11648/j.avs.20140204.15
Page(s) 109-117
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Protein Content, Electrophoretic Pattern, Edible Muscles, Crustaceans Species, Mollusks Species

References
[1] R., Mcpherson and K., Brwon, The bioaccumulation of Cd by the blue swimmer crab Porrtunus pelagicus L. Sci Total Environ., 279:223-230, 2001.
[2] P, Bjerregaar and M.H, Depledge, Trace metals concentrations and contents in the tissuses of the shore crab : effects of size and tissue hydration. Mar.Biol., 141:741-752,2002.
[3] W.S., Sallam, T. A., Temraz,. and H. R., Gabar, “Biochemical compositions and heavy metals accumulation in some commercial crustaceans from the Mediterranean coast off Port Said , Egypt”. J. Egypt. Ger. Soc. Zool. 51 D: 127-14 , 2006.
[4] A. Rangappa, T. Raj Kumar, P. Jaganmohan and M. Srinivasulu Reddy, Studies on the Proximal Composition of Freshwater Prawns Macrobrachium rosenbergii and Macrobrachium malcomsonii. World J. Fish & Marine Sci., 4 (2): 218-222, 2012.
[5] H.A., Abdel-Salam, Amino acid composition in the muscles of male and female commercially important crustaceans from Egyptian and Saudi Arabia coasts. Amer. J. Biosci, 2(2): 70-78, 2014.
[6] G.K. Dinakaran, P. Soundarapandan, and S.K. Chandra, Proximate analysis of edible Palaeomonid prawn, Macrobrachium idae. J. Biological Sciences. 1(3): 78-82 , 2009.
[7] F.A.R., Ehigiator and E.A., Oterai,., “Chemical Composition and Amino Acid Profile of a Caridean Prawn (Macrobra chium vollenhoveanil) from Ovia river and tropical periwinkle (Tympanonus fusctus) from Benin river”. Edo state , Nigeria.”. IJRRAS, 11 (1):162-166, 2012.
[8] I. Ekin, , M. Ba han and e en, R Possible seasonal variation of the fatty acid composition from Melanopsispraemorsa(L., 1758) (Gastropoda: Prosobranchia), from southeast Anatolia, Turkey. Turk J Biol. 35: 203, 213,2011.
[9] J. M., Salman and A. J., Nasar, Total Lipids and total protein in two Mollusca species as environmental biomarker of pollution in Euphrates River, Iraq. Int.J.Curr.Microbiol.App.Sci 2(10): 207-214, 2013.
[10] Zlatanos, S., Laskaridis, K., Feist, C., & Sagredos, A., Proximate composition, fatty acid analysis and protein digestibility-corrected amino acid score of three Mediterranean cephalopods. Molecular Nut. Food Res., 50, 967–970, 2006.
[11] A. V. , Sykes,*, A. R., Oliveira, P. M., Domingues c, C. M., Cardoso b, J. P., Andrade, M. L. Nunes, Assessment of European cuttlefish (Sepia officinalis, L.) nutritional value and freshness under ice storage using a developed Quality Index Method (QIM) and biochemical methods, LWT - Food Science and Technology 42 : 424–432, 2009.
[12] E, Schoffeniesls and R., Giles, “Nitrogenous constituents and nitrogen metabolism in arthropods”. In chemical Zoology, M., Fokin and B. T., Scheer (Eds). Academic press, New York, pp 199-277,1970b.
[13] M., Okuzumi, and T. Fujii, “Nutritional and functional properties of squid and cuttlefish”. National Cooperative. Association of Squid Processors, California. Pp 223, 2000.
[14] M. Sudhakar, K. Manivannan and P. Soundrapandian, Nutritive value of hard and soft shell crabs of Portunus sanguinolentus (Herbst) ”. Int. J. Anim. Veter. Adv., 1(2): 44-48, 2009.
[15] J.S., Diana, “An experimental analysis of the metabolic rate and food utilization of northern pike”. Comp. Biochem. Toxicol., 59, 989-993, 1982.
[16] P., Anilkumar, and, G. Meenaksh, Ascorbate, “Effect on Proteinontent during nickel intoxication in the freshwater bivalve”. Lamellidens corrianus. Biosci.Discov. 3(2): 270-274, 2012.
[17] M.B., New, The role freshwater prawns in sustainable aquaculture. Freshwater prawns International symposium, kerala Agriculture University, Kochi, India, pp: 10-13, 2003.
[18] Y. Suneetha, , P. Sreenivasula Reddy, P. Naga Jyothi symposium on Invertebrate Reproduction. pp: 7- and M. Srinivasulu Reddy, 2009. Proximal changes New century printers Penaeid prawn Penaeus monodon, World J. Fish and Marine Sci., 1(4): 333-337.
[19] Samuel, M.J., T. Kannupandi and Soundarapandian, Nutritional effects on male for total lipids. isolation and quantification of Macrobrachium malcolmsonii (H. Milne Edwards). J. Expl. Mar. Ecol., 12: 103-118. P. simple method for the. J. Biological Aquaculture, 172(3): 327-333, 1999.
[20] A.A, Kocatas and T., Katagan, On stomatopoda from Turkey with the first record of Rissoles Pallidus from the Turkish Fauna. Crustaceana. 68(5): 649-652, 1995.
[21] S. A. H ., HAMDI , “Muscle and exoskeleton extracts analysis of both fresh and marine crustaceans P. clarki and E. massavensis. African” J. Pharm. 5(13):1589-1597, 2011.
[22] W., Lawery, H Daughaday, N.J Rosebroughady, and Field, W.S, “ Determination of cerebral protein using Folin phenol reagent, ” J. lab. Clin Med.,39: 663-665,1952.
[23] UK Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4, ” Nature, 227: 680-685,1970.
[24] P. Sriket, S Benjakul, W .Visessanguan, and KijroongrojanaK. , Comparative studies on 476 “chemical composition and thermal properties of black tiger shrimp (Penaeus monodon) and white shrimp (Penaeus vannamei) meats”, Food Chem.103:1199-1207, 2007.
[25] Kyomo, J., Analysis of the relationship between gonads and hepatopancreas in males and females of the crab Sesarma intermedia, with reference to resource use and reproduction. Marine Bio. 97, 87–93, 1988.
[26] Jeckel, W.H., Moreno, J.E., Moreno, V.J., Biochemical composition, lipid classes and fatty acids in the male reproductive system of the shrimp Pleoticus muelleri bate. Comparative Biochemistry and Physiology 93B, 807–811, 1989.
[27] R. Rosa and M.L. Nunes, Biochemical composition of deep-sea decapod crustaceans with two different benthic life strategies off the Portug uese south coast. Deep-Sea Res.I 50 ,119–130, 2003.
[28] H.M., Mona, S.N., Geasa, Sharshar, and M.K. M. E. Morsy, “Chemical composition of freshwater crayfish Erugosquilla massavensis and its nutritive values. Egypt”. J. aquatic. Biol. fish.,4(1):19-34, 2000.
[29] H.A.,Abdel-Salam, and A.H. S, Hamdi, “Bio chemical compositions and heavy metals accumulation capacity of the of the marine mantis shrimp Erugosquilla massavensis”. Soc. ZOO., 61:199-214, 2011.
[30] S.A Hamdi, and K. H. Zaghloul, ,“Evaluation of the crawfish Procambarus clarkli as a cheaper source of human diet in comparison with two marine shrimps in Egypt., J. Egypt. Ger. Soc. Zool. 50 D: 153-174, 2006.
[31] K.M. Ajith, , Studies on the proximate composition of the prawn Macrobrachium idella (Hilgendorf). M. Phil Thesis, Annamalai University, 1990.
[32] H.A., Tag El Din, M. M. ,Habashy and H. H., Sultan, “Residues ues of some heavy metals and hormones in fresh water prawn (Macrobrac uhium rosenbergi and marine shrimp Penaeus semisulcatus with reference to the nutritive value”. World J. ZOO.4(3):205-215, 2009.
[33] H.A., Abdel-Salam, “Evaluation of nutritional quality of commercially cultured Indian white shrimp Penaeus indicus”. Inter. J. Nutr. and Food Sci.; 2(4): 160-166, 2013.
[34] H.A., Abdel-Salam, Amino acid composition in the muscles of male and female commercially important crustaceans from Egyptian and Saudi Arabia coasts Amer. J. Biosci.; 2(2): 70-78, 2014.
[35] G.K. Dinakaran, P. Soundarapandan, and S.K. Chandra, Proximate analysis of edible Palaeomonid prawn, Macrobrachium idae. J. Biol. Sci.. 1(3): 78-82, 2009.
[36] F , Habib, A.,Zarrien, S. Abid ALI , S. Ghazala, Biochemical composition of hemolymph, hepatopancreas, ovary and muscle 3 during ovarian maturation in the penaeid shrimps, Fenneropenaeus 4 merguiensis and F. penicillatus (Crustacea: Decapoda) E-mail address: habibfatima59@yahoo.com 23, 2013.
[37] A.M., Bello-Olusoji, O.A., BalogunFagbenro, and N. Ugbaja, “Food and feeding studies of the African river prawn. In: Proceedings of Fish and Shellfish Larviculture Symposium. Lavens P., Japan E. and Roelants, I. (Eds.) ”. Europ. Aquac. Soc., Special Publication. 24: 425-427, 1995.
[38] Cartes, J.E., Diets of, and trophic resources exploited by, bathyal penaeoidean shrimps from the western Mediterranean. Marine Freshwater Res. 46, 889–896, 1995.
[39] J.E., Cartes, P., Abello, Comparative feeding habits of polychelid lobsters in the Western Mediterranean deep-sea communities. Marine Ecology Progress Series 84, 139–150, 1992.
[40] M.B., New, The role freshwater prawns in sustainable aquaculture. Freshwater prawns International symposium, Kerala Agriculture University, Kochi, India, pp: 10-13, 2003.
[41] Y., Suneetha, P. Sreenivasula Reddy, P. Naga Jyothiand M. Srinivasulu Reddy, Proximal changes during reproduction process of the Penaeid prawnPenaeus monodon. World J. Fish and Marine Sci., 1(4): 333-337, 2009.
[42] M.J., Samuel, T. Kannupandi andreproductive performance in the freshwater prawnMacrobrachium malcolmsonii (H. Milne Edwards). Aquaculture, 172(3): 327-333,1999.
[43] D.R., Fielder, K., RangaRao, and M.S.,. Tingerman, diversity of hemocyanin components in the dimorphic variant of the fiddlercrab Uca pugilator, as revealed by disc electrophoresis Comp. Biochem. Physiol., 39: 291-297,1971.
[44] R.G. Adiyodi, and Adiyodi, K.G. , Hepatopancreas of Paratelphusa hydrodromous (Herbst): Histophysiology and the pattern of proteins in relation to reproduction and molt, Biol 6, 144—I47,1972.
[45] F.Y., Lee, T.W.Shih, and C.F. , Chang, Isolation and characterization of the female specific protein (Vitellogenin) in mature female hemolymph of the fresh water Macrobrachium rosenbergii comparison with ovarian vitellin, General and Comp. Endocrinol., 108: 406-416, 1997.
[46] S.A Hamdi, and K. H. Zaghloul, ,“Evaluation of the crawfish Procambarus clarkli as a cheaper source of human diet in comparison with two marine shrimps in Egypt., J. Egypt. Ger. Soc. Zool. 50 D: 153-174, 2006.
[47] H.A .,Abdel-Salam, and A.H. S, Hamdi, Heavy metals monitoring using commercially important crustaceans and mollusks collected from Egyptian and Saudi Arabia coasts. Animal and Veterinary Sci. 2(3): 49-61, 2014.
Cite This Article
  • APA Style

    Hala Ali Abdel-Salam. (2014). Protein and Electrophoretic Analysis of Edible Muscle of Commercially Important Crustaceans and Mollusks Species from Egyptian and Saudi Arabia Costs. Animal and Veterinary Sciences, 2(4), 109-117. https://doi.org/10.11648/j.avs.20140204.15

    Copy | Download

    ACS Style

    Hala Ali Abdel-Salam. Protein and Electrophoretic Analysis of Edible Muscle of Commercially Important Crustaceans and Mollusks Species from Egyptian and Saudi Arabia Costs. Anim. Vet. Sci. 2014, 2(4), 109-117. doi: 10.11648/j.avs.20140204.15

    Copy | Download

    AMA Style

    Hala Ali Abdel-Salam. Protein and Electrophoretic Analysis of Edible Muscle of Commercially Important Crustaceans and Mollusks Species from Egyptian and Saudi Arabia Costs. Anim Vet Sci. 2014;2(4):109-117. doi: 10.11648/j.avs.20140204.15

    Copy | Download

  • @article{10.11648/j.avs.20140204.15,
      author = {Hala Ali Abdel-Salam},
      title = {Protein and Electrophoretic Analysis of Edible Muscle of Commercially Important Crustaceans and Mollusks Species from Egyptian and Saudi Arabia Costs},
      journal = {Animal and Veterinary Sciences},
      volume = {2},
      number = {4},
      pages = {109-117},
      doi = {10.11648/j.avs.20140204.15},
      url = {https://doi.org/10.11648/j.avs.20140204.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20140204.15},
      abstract = {Crustaceans and mollusks are important components of the aquatic fauna. Furthermore, crustaceans and mollusks consumption have been increasing worldwide during the past decades. So, therefore, the present study was investigated the variation between total protein content and protein electrophoretic pattern in edible muscle of commercially important crustaceans (Erugosquilla massavensis, Peaneus semisulcatus, Metapenaeus monoceros and Portunus pelagicus) and mollusks (Sepia spp., Cardium edule) as an attempt to rank them as an alternative rich animal protein source for human. The recorded data declare that the highest protein percentage was observed in males E. massavensis followed by Sepia spp then P. pelagicus and shrimps. On the other hand, females E. massavensis and bivalve mollusks (C. edule) had less total protein contents in their muscles, additionally, the recorded data showed a variation in band numbers and estimated molecular weight of muscle protein between sexes of each species and among the studied species. The results of the present work clearly indicate that there are differences in the protein structure of the muscles in different species of crustaceans and mollusks with reference to the protein fractions and their molecular weights.},
     year = {2014}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Protein and Electrophoretic Analysis of Edible Muscle of Commercially Important Crustaceans and Mollusks Species from Egyptian and Saudi Arabia Costs
    AU  - Hala Ali Abdel-Salam
    Y1  - 2014/07/20
    PY  - 2014
    N1  - https://doi.org/10.11648/j.avs.20140204.15
    DO  - 10.11648/j.avs.20140204.15
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
    SP  - 109
    EP  - 117
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20140204.15
    AB  - Crustaceans and mollusks are important components of the aquatic fauna. Furthermore, crustaceans and mollusks consumption have been increasing worldwide during the past decades. So, therefore, the present study was investigated the variation between total protein content and protein electrophoretic pattern in edible muscle of commercially important crustaceans (Erugosquilla massavensis, Peaneus semisulcatus, Metapenaeus monoceros and Portunus pelagicus) and mollusks (Sepia spp., Cardium edule) as an attempt to rank them as an alternative rich animal protein source for human. The recorded data declare that the highest protein percentage was observed in males E. massavensis followed by Sepia spp then P. pelagicus and shrimps. On the other hand, females E. massavensis and bivalve mollusks (C. edule) had less total protein contents in their muscles, additionally, the recorded data showed a variation in band numbers and estimated molecular weight of muscle protein between sexes of each species and among the studied species. The results of the present work clearly indicate that there are differences in the protein structure of the muscles in different species of crustaceans and mollusks with reference to the protein fractions and their molecular weights.
    VL  - 2
    IS  - 4
    ER  - 

    Copy | Download

Author Information
  • Department of Zoology, Faculty of Science, Cairo University, Egypt

  • Sections