Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs

Fábio Alessandro Pieri; Vitor de Oliveira Silva; Abelardo Silva Junior; Maria Aparecida Scatamburlo Moreira

doi:doi:10.11648/j.avs.20180602.11

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Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs

Fábio Alessandro Pieri, Vitor de Oliveira Silva, Abelardo Silva Junior, Maria Aparecida Scatamburlo Moreira

Received: 6 April 2018 Accepted: 23 April 2018 Published: 15 May 2018

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Abstract

Periodontal disease, which affects the support structures and protection of teeth, is the main disease found in dogs, reaching about 85% prevalence in animals over 4 years old. The most important bacteria in the initial colonization of human dental plaque are Streptococcus, which produce exopolysaccharides that facilitate fixation of the bacteria to the surfaces. Some studies have sought to identify the dental plaque microbiota in dogs using non-selective culture media, and have reported small amounts of Streptococcus, however they did not provide ideal conditions for the growth of this bacteria. The aim of this study was to isolate and identify the initial dental plaque microbiota of dogs cultivable in a selective medium for Streptococcus, and verify the presence of this genus. Genotypic identification of the isolates were carried out by partial 16S rDNA sequencing. A total of 179 strains were obtained from the plaque of 10 dogs without periodontal disease. The technique employed allowed 15 different microbial groups to be isolated, including the genus Streptococcus. This represented 33 (18.4%) of 179 isolates, thus illustrating the partially selective ability of Mitis Salivarius Agar for samples of dental plaque in dogs.

DOI	10.11648/j.avs.20180602.11
Published in	Animal and Veterinary Sciences (Volume 6, Issue 2, March 2018)
Page(s)	21-26
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Dogs, Oral Microbiology, Dental Plaque, Microbiota, Bacteria, 16S rDNA

References

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APA Style

Fábio Alessandro Pieri, Vitor de Oliveira Silva, Abelardo Silva Junior, Maria Aparecida Scatamburlo Moreira. (2018). Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs. Animal and Veterinary Sciences, 6(2), 21-26. https://doi.org/10.11648/j.avs.20180602.11

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ACS Style

Fábio Alessandro Pieri; Vitor de Oliveira Silva; Abelardo Silva Junior; Maria Aparecida Scatamburlo Moreira. Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs. Anim. Vet. Sci. 2018, 6(2), 21-26. doi: 10.11648/j.avs.20180602.11

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AMA Style

Fábio Alessandro Pieri, Vitor de Oliveira Silva, Abelardo Silva Junior, Maria Aparecida Scatamburlo Moreira. Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs. Anim Vet Sci. 2018;6(2):21-26. doi: 10.11648/j.avs.20180602.11

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@article{10.11648/j.avs.20180602.11,
  author = {Fábio Alessandro Pieri and Vitor de Oliveira Silva and Abelardo Silva Junior and Maria Aparecida Scatamburlo Moreira},
  title = {Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs},
  journal = {Animal and Veterinary Sciences},
  volume = {6},
  number = {2},
  pages = {21-26},
  doi = {10.11648/j.avs.20180602.11},
  url = {https://doi.org/10.11648/j.avs.20180602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20180602.11},
  abstract = {Periodontal disease, which affects the support structures and protection of teeth, is the main disease found in dogs, reaching about 85% prevalence in animals over 4 years old. The most important bacteria in the initial colonization of human dental plaque are Streptococcus, which produce exopolysaccharides that facilitate fixation of the bacteria to the surfaces. Some studies have sought to identify the dental plaque microbiota in dogs using non-selective culture media, and have reported small amounts of Streptococcus, however they did not provide ideal conditions for the growth of this bacteria. The aim of this study was to isolate and identify the initial dental plaque microbiota of dogs cultivable in a selective medium for Streptococcus, and verify the presence of this genus. Genotypic identification of the isolates were carried out by partial 16S rDNA sequencing. A total of 179 strains were obtained from the plaque of 10 dogs without periodontal disease. The technique employed allowed 15 different microbial groups to be isolated, including the genus Streptococcus. This represented 33 (18.4%) of 179 isolates, thus illustrating the partially selective ability of Mitis Salivarius Agar for samples of dental plaque in dogs.},
 year = {2018}
}

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TY  - JOUR
T1  - Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs
AU  - Fábio Alessandro Pieri
AU  - Vitor de Oliveira Silva
AU  - Abelardo Silva Junior
AU  - Maria Aparecida Scatamburlo Moreira
Y1  - 2018/05/15
PY  - 2018
N1  - https://doi.org/10.11648/j.avs.20180602.11
DO  - 10.11648/j.avs.20180602.11
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 21
EP  - 26
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20180602.11
AB  - Periodontal disease, which affects the support structures and protection of teeth, is the main disease found in dogs, reaching about 85% prevalence in animals over 4 years old. The most important bacteria in the initial colonization of human dental plaque are Streptococcus, which produce exopolysaccharides that facilitate fixation of the bacteria to the surfaces. Some studies have sought to identify the dental plaque microbiota in dogs using non-selective culture media, and have reported small amounts of Streptococcus, however they did not provide ideal conditions for the growth of this bacteria. The aim of this study was to isolate and identify the initial dental plaque microbiota of dogs cultivable in a selective medium for Streptococcus, and verify the presence of this genus. Genotypic identification of the isolates were carried out by partial 16S rDNA sequencing. A total of 179 strains were obtained from the plaque of 10 dogs without periodontal disease. The technique employed allowed 15 different microbial groups to be isolated, including the genus Streptococcus. This represented 33 (18.4%) of 179 isolates, thus illustrating the partially selective ability of Mitis Salivarius Agar for samples of dental plaque in dogs.
VL  - 6
IS  - 2
ER  -

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Author Information

Fábio Alessandro Pieri

Basic Life Sciences Department, Life Sciences Institute, Federal University of Juiz de Fora, Governador Valadares, Brazil
Vitor de Oliveira Silva

Veterinary Department, Health and Biological Sciences Center, Federal University of Vi?osa, Vi?osa, Brazil
Abelardo Silva Junior

Veterinary Department, Health and Biological Sciences Center, Federal University of Vi?osa, Vi?osa, Brazil
Maria Aparecida Scatamburlo Moreira

Veterinary Department, Health and Biological Sciences Center, Federal University of Vi?osa, Vi?osa, Brazil

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APA Style

Fábio Alessandro Pieri, Vitor de Oliveira Silva, Abelardo Silva Junior, Maria Aparecida Scatamburlo Moreira. (2018). Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs. Animal and Veterinary Sciences, 6(2), 21-26. https://doi.org/10.11648/j.avs.20180602.11

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ACS Style

Fábio Alessandro Pieri; Vitor de Oliveira Silva; Abelardo Silva Junior; Maria Aparecida Scatamburlo Moreira. Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs. Anim. Vet. Sci. 2018, 6(2), 21-26. doi: 10.11648/j.avs.20180602.11

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AMA Style

Fábio Alessandro Pieri, Vitor de Oliveira Silva, Abelardo Silva Junior, Maria Aparecida Scatamburlo Moreira. Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs. Anim Vet Sci. 2018;6(2):21-26. doi: 10.11648/j.avs.20180602.11

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@article{10.11648/j.avs.20180602.11,
  author = {Fábio Alessandro Pieri and Vitor de Oliveira Silva and Abelardo Silva Junior and Maria Aparecida Scatamburlo Moreira},
  title = {Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs},
  journal = {Animal and Veterinary Sciences},
  volume = {6},
  number = {2},
  pages = {21-26},
  doi = {10.11648/j.avs.20180602.11},
  url = {https://doi.org/10.11648/j.avs.20180602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20180602.11},
  abstract = {Periodontal disease, which affects the support structures and protection of teeth, is the main disease found in dogs, reaching about 85% prevalence in animals over 4 years old. The most important bacteria in the initial colonization of human dental plaque are Streptococcus, which produce exopolysaccharides that facilitate fixation of the bacteria to the surfaces. Some studies have sought to identify the dental plaque microbiota in dogs using non-selective culture media, and have reported small amounts of Streptococcus, however they did not provide ideal conditions for the growth of this bacteria. The aim of this study was to isolate and identify the initial dental plaque microbiota of dogs cultivable in a selective medium for Streptococcus, and verify the presence of this genus. Genotypic identification of the isolates were carried out by partial 16S rDNA sequencing. A total of 179 strains were obtained from the plaque of 10 dogs without periodontal disease. The technique employed allowed 15 different microbial groups to be isolated, including the genus Streptococcus. This represented 33 (18.4%) of 179 isolates, thus illustrating the partially selective ability of Mitis Salivarius Agar for samples of dental plaque in dogs.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs
AU  - Fábio Alessandro Pieri
AU  - Vitor de Oliveira Silva
AU  - Abelardo Silva Junior
AU  - Maria Aparecida Scatamburlo Moreira
Y1  - 2018/05/15
PY  - 2018
N1  - https://doi.org/10.11648/j.avs.20180602.11
DO  - 10.11648/j.avs.20180602.11
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 21
EP  - 26
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20180602.11
AB  - Periodontal disease, which affects the support structures and protection of teeth, is the main disease found in dogs, reaching about 85% prevalence in animals over 4 years old. The most important bacteria in the initial colonization of human dental plaque are Streptococcus, which produce exopolysaccharides that facilitate fixation of the bacteria to the surfaces. Some studies have sought to identify the dental plaque microbiota in dogs using non-selective culture media, and have reported small amounts of Streptococcus, however they did not provide ideal conditions for the growth of this bacteria. The aim of this study was to isolate and identify the initial dental plaque microbiota of dogs cultivable in a selective medium for Streptococcus, and verify the presence of this genus. Genotypic identification of the isolates were carried out by partial 16S rDNA sequencing. A total of 179 strains were obtained from the plaque of 10 dogs without periodontal disease. The technique employed allowed 15 different microbial groups to be isolated, including the genus Streptococcus. This represented 33 (18.4%) of 179 isolates, thus illustrating the partially selective ability of Mitis Salivarius Agar for samples of dental plaque in dogs.
VL  - 6
IS  - 2
ER  -

Copy | Download