Animal and Veterinary Sciences

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Comparison of Some Hematological Parameters Between Horses in an Endurance Competition

Received: 13 November 2016    Accepted: 28 November 2016    Published: 06 January 2017
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Abstract

This study conducted to comparison of some blood constituents between horses participated an endurance racing. Jugular vein puncture was done to collect blood from15 horses (1 gelding, 2 mares, and 12 male). These horses had been classified to three breeds include 12Kurd, 1 pure Arab, 2cross-breed and participated in the endurance racing of 0 (before competition), 15 (After first loop) and 30 km (final loop). Horses were classified in three age-groups include below 5(n=1), 5-10 (n=10) and above 10years of age (n=4). Hematocrit and Hemoglobin percentage, Total protein concentration, Red blood cell and White blood cell counting, glucose and cortisol level were measured at before starting competition (0), 15 and 30 km. Data was analyzed by SPSS (version 22). Results showed significant difference in glucose level after 30 km (P<0.05) but not after 15km (P>0.05). No significant difference was observed between cortisol concentration and age groups (P>0.05) but, there was significant difference between cortisol concentration and gender (P<0.05). Breed had influenced on HT, Hb, TP and WBC (P<0.05). Otherwise breed had no effect on RBC, glucose level and cortisol concentration (P>0.05). HT and WBC counting were higher in Arab, cross breed and Kurd respectively. Hemoglobin percentage was found higher in Arab, Kurd and Cross breed respectively. Total protein concentration in Arab was higher than Cross breed and Kurd. In conclusion, in short distance endurance competition of horses, stress is not effect on metabolism of animal, furthermore, gender and breed could important in blood parameters alterations.

DOI 10.11648/j.avs.20160406.13
Published in Animal and Veterinary Sciences (Volume 4, Issue 6, November 2016)
Page(s) 97-102
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Horse, Blood, Endurance

References
[1] Hinchcliiff KW, Geor RJ: The horse as an athlete: a physiological overview. In, Hinchcliiff KW (Ed): Equine exercise physiology: the science of the exercise in the athletic horse. 2-10, Philadelphia, Saunders, 2008.
[2] Fan YK, Hsu JC, Peh HC, Tsang CL, Cheng SP, Chiu SC, Ju JC: The effects of endurance training on the hemogram of the horse. Asian-Aust JAnimSci, 15 (9): 1348-1353, 2002.
[3] Teixeira-Neto AR, Ferraz GC, Moscardini ARC, Albernaz RM, Gondin MR, Queiroz-Neto A: Do hematologic constituents really increase due to endurance exercise in horses?. Pesq Vet Bras, 32 (9): 951-956, 2012.
[4] Waller AP, Heigenhauser GJF, Geor RJ, Spriet LL, Lindinger MI: Fluid and electrolyte supplementation after prolonged moderate-intensity exercise enhances muscle glycogen resynthesis in Standardbred horses. J Appl Physiol, 106: 91-100, 2009.
[5] Wickler SJ, Foss MA: Veterinary aspects of endurance riding. In, Hinchcliff KW, Kaneps AK, Geor RJ (Eds): Equine sports and medicine-basic and clinical sciences of the equine athlete. 1105-1117, London, Saunders, 2004.
[6] Annamaria N, Sue JD, Jane KM: A veterinary review of endurance riding as an international competitive sport. Vet J, 1-6, 2012.
[7] McKeever KH: Body luids and electrolytes: Responses to exercise and training. In, Hinchcliff KW, Geor RJ, Kaneps AJ (Eds): Equine Exercise Physiology: The science of exercise in the athletic horse. 328-396, Saunders, London, 2008.
[8] Kingston JK: Hematologic and serum biochemical responses to exercise and training. In, Hinchcliff KW, Geor RJ, Kaneps AJ (Eds): Equine Exercise Physiology: The science of exercise in the athletic horse. 398-422, Saunders, London, 2008.
[9] Sneddon JC: Physiological effects of hypertonic dehydration on body fluid pools in arid-adapted mammals. How do arab-based horses compare?. Comp BiochemPhysiol, A: Comp Physiol, 104 (2): 201–213, 1993.
[10] Kingston JK: Hematologic and serum biochemical responses to exercise and training. In, Hinchcliff KW, Kaneps AJ, Geor RJ (Eds): Equine Sports Medicine and Surgery: Basic and clinical sciences of the equine athlete. 939-948, Saunders, London, 2004.
[11] Linder A, Signorini R, Bredo L, Arn LE, Mancini R: Effect of conditioning horses 1, 2, 3 X a week with 2 intervals of 100m at near maximal speed on biochemical variables in the blood. In, Proceeding of the 7th International Conference on Equine Exercise Physiology. Fontainebleau, France, 24, 2006,
[12] Cottin F, Barrey E, Lopes P, Billat VL: Effect of fatigue during five successive heats (800m at high velocity) and recovery runs on heart rate variability in Standardbreds. In, Proceeding of the 7th International Conference on Equine Exercise Physiology. Fontainebleau, France, 68, 2006.
[13] Bashir A, Rasedee A: Plasma catecholamine, sweat electrolytes and physiological responses of exercised normal, partial, anhidrotic and anhidrotic horses. Am J Anim Vet Sci, 4: 26-31, 2009.
[14] Rasedee A: Effects of race distance on physical, hematological and biochemical parameters of endurance horses. Am J Anim Vet Sci, 5 (4): 244-248, 2010.
[15] Valberg SJ: Approach the horse with a suspected myopathy. In, Proceeding of the 11th International Congress of World Equine Veterinary Association.1-15, 2009.
[16] Snow DH, Harris RC, MacDonald IA, Forster CD, Marlin DJ: Effects of high-intensity exercise on plasma catecholamines in the thoroughbred horse. Equine Vet J, 24: 462-467, 1992.
[17] Waller AP, Heigenhauser GJF, Geor RJ, Spriet LL, Lindinger MI: Fluid and electrolyte supplementation after prolonged moderate-intensity exercise enhances muscle glycogen resynthesis in Standardbred horses. J Appl Physiol, 106:91-100, 2009.
[18] 18. Lawan A, Noraniza MA, Rasedee A, Bashir A: Effect of age and performance on physical, hematological, and biochemical parameters in endurance horses. J Equine Vet Sci, 1-6, 2012.
[19] Kingston JK: Hematologic and serum biochemical responses to exercise and training. In, Hinchcliff KW, Kaneps AJ, Geor RJ (Eds): Equine Sports Medicine and Surgery: Basic and clinical sciences of the equine athlete. 939-948, W. B. Saunders, London, 2004.
[20] Naylor JR, Bayly WM, Schott HC, Gollnick PD, Hodgson DR: Equine plasma and blood volumes decrease with dehydration but subsequently increase with exercise. J Appl Physiol. 75: 1002-1008, 1993.
[21] Scoppetta F, Tartaglia M, Renzone G, Avellini L, Gaiti A, Scaloni A, Chiaradia E: Plasma protein changes in horse after prolonged physical exercise: A proteomic study. J Proteomics, 19; 75 (14): 4494-504, 2012.
[22] De Waal A, Potgieter FJ: Evaluation of an electronic device used as a quick screening method to detect changes in the red cell content of the blood in horses participating in endurance trail rides. Vet Res Commun, 15 (4): 309-14. 23, 1991.
[23] Jones, WE: The role of blood in equine performance. In, Jones WE (Ed): Equine Sport Medicine. Lea &Febiger, Philadelphia, USA, 35-52, 1989.
[24] Rose RJ, Hodgson DR: Hematological and plasma biochemical parameters in endurance horses during training. Equine Vet J, 14: 144-148, 1982.
[25] Dybdal NO, Gribble D, Madigan JE, Stabenfeldt GH: Alterations in plasma corticosteroids, insulin and selected metabolites in horses used in endurance rides. Equine Vet J, 12: 137-140, 1980.
[26] Jensen-Waern M, Lindberg A, Johannisson A, Gröndahl G, Lindgren JA, Essén-Gustavsson B; The effects of an endurance ride on metabolism and neutrophil function. Equine Vet J Suppl, 30: 605-9, 1999.
[27] Schalm OW: Equine Hematology: Part 2 Thrombocytes and leukocytes. Equine Practice, 1: 24-35, 1979.
[28] Chiaradia E, Gaiti A, Terracina L, Avellini L: Effect of submaximal exercise on horse homocysteinaemia: possible implications for immune cells. Res Vet sci, 79 (1): 9–14, 2005.
[29] Gurgoze SY, Icen H: The influence of age on clinical biochemical parameters in pure-bred Arabian mares. J Equine Vet Sci, 30: 569-73, 2010.
[30] Vincze A, Szabó CS, Heves Á, Veres S, Üto D, Babinszky L: Effect of age and event on post exercise values of blood biochemical parameters in show jumping horses. Acta AgrariaKaposváriensis, 14: 185-91, 2010.
[31] Hayashi, T, Wojtaszewski JFP, Goodyear J: Exercise regulation of glucose transport in skeletal muscle. Am J PhysioloEndocrinMetab, 273: 1036-1051, 1997.
[32] Tomás E, Zorzano A, Ruderman NB: Exercise and insulin signaling: a historical perspective. J Appl Physiol, 93: 765-772, 2002.
[33] Hirshman, MF, Wallberg-Henriksson H, Wardzala LJ, Horton ED, Horton ES: Acute exercise increases the number of plasma membrane glucose transporters in rat skeletal muscle. FEBS letters, 238: 235-239, 1988.
[34] Prenen H, Stefanq C, Landuyt B, Vermaelen P, Debiec-RychterImatinib M: Mesylate inhibits glucose uptake in gastrointestinal stromal tumor cells by downregulation of the glucose transporters recruitment to the plasma membrane. Am J Biochem Biotech, 1: 95-102, 2005.
[35] Alhusseini NF, Belacy NA, Kasem EM, Allam MM: Effect of exercise training on adiponectin receptor expression and insulin resistance in mice fed a high fat diet. Am J Biochem Biotech, 6: 77-83, 2010.
[36] Peeters M, Closson C, Beckers J: Rider and Horse Salivary Cortisol Levels during Competition and Impact on Performance. J Equine Vet Sci, 33 (3): 155–160, 2013.
[37] Hyyppä S: Endocrinal responses in exercising horses. Livest Prod Sci, 92(2):113–121, 2005.
[38] Ferlazzo AM, Bruschetta G, DiPietro P, Medica P: The influence of age on plasma serotonin levels in Thoroughbred horses. Livest Sci, 147 (1–3): 203–207, 2012.
[39] Desmecht D, Linden A, Amory H, Art T, Lekeux P: Relationship of plasma lactate production to cortisol release following completion of different types of sporting events in horses. Vet Res Commun, 20 (4): 371-9, 1996.
[40] Lacerda L, Campos R, Sperb M, Soares E, Barbosa P, Godinho E, Ferreira R, Santos V, Gonzalez FD: Hematologic and biochemical parameters in three high performance horse breeds from southern Brazil. Archives Vet Sci, 11: 40-4, 2006.
[41] Schmidta A, Jörg A, Möstlc E, Müllera J, Auricha C: Changes in cortisol release and heart rate and heart rate variability during the initial training of 3-year-old sport horses. Hoem Behav, 58 (4): 628–636, 2010.
Author Information
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

  • Department of Basic Sciences, Faculty of Veterinary Medicine, University of Shiraz, Shiraz, Iran

  • Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

  • Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

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    Fereydon Rezazadeh, Neda Hosseinzadeh, Raziollah Jafari Jozani, Nima Dianati. (2017). Comparison of Some Hematological Parameters Between Horses in an Endurance Competition. Animal and Veterinary Sciences, 4(6), 97-102. https://doi.org/10.11648/j.avs.20160406.13

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    Fereydon Rezazadeh; Neda Hosseinzadeh; Raziollah Jafari Jozani; Nima Dianati. Comparison of Some Hematological Parameters Between Horses in an Endurance Competition. Anim. Vet. Sci. 2017, 4(6), 97-102. doi: 10.11648/j.avs.20160406.13

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    AMA Style

    Fereydon Rezazadeh, Neda Hosseinzadeh, Raziollah Jafari Jozani, Nima Dianati. Comparison of Some Hematological Parameters Between Horses in an Endurance Competition. Anim Vet Sci. 2017;4(6):97-102. doi: 10.11648/j.avs.20160406.13

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  • @article{10.11648/j.avs.20160406.13,
      author = {Fereydon Rezazadeh and Neda Hosseinzadeh and Raziollah Jafari Jozani and Nima Dianati},
      title = {Comparison of Some Hematological Parameters Between Horses in an Endurance Competition},
      journal = {Animal and Veterinary Sciences},
      volume = {4},
      number = {6},
      pages = {97-102},
      doi = {10.11648/j.avs.20160406.13},
      url = {https://doi.org/10.11648/j.avs.20160406.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.avs.20160406.13},
      abstract = {This study conducted to comparison of some blood constituents between horses participated an endurance racing. Jugular vein puncture was done to collect blood from15 horses (1 gelding, 2 mares, and 12 male). These horses had been classified to three breeds include 12Kurd, 1 pure Arab, 2cross-breed and participated in the endurance racing of 0 (before competition), 15 (After first loop) and 30 km (final loop). Horses were classified in three age-groups include below 5(n=1), 5-10 (n=10) and above 10years of age (n=4). Hematocrit and Hemoglobin percentage, Total protein concentration, Red blood cell and White blood cell counting, glucose and cortisol level were measured at before starting competition (0), 15 and 30 km. Data was analyzed by SPSS (version 22). Results showed significant difference in glucose level after 30 km (P<0.05) but not after 15km (P>0.05). No significant difference was observed between cortisol concentration and age groups (P>0.05) but, there was significant difference between cortisol concentration and gender (P<0.05). Breed had influenced on HT, Hb, TP and WBC (P<0.05). Otherwise breed had no effect on RBC, glucose level and cortisol concentration (P>0.05). HT and WBC counting were higher in Arab, cross breed and Kurd respectively. Hemoglobin percentage was found higher in Arab, Kurd and Cross breed respectively. Total protein concentration in Arab was higher than Cross breed and Kurd. In conclusion, in short distance endurance competition of horses, stress is not effect on metabolism of animal, furthermore, gender and breed could important in blood parameters alterations.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Comparison of Some Hematological Parameters Between Horses in an Endurance Competition
    AU  - Fereydon Rezazadeh
    AU  - Neda Hosseinzadeh
    AU  - Raziollah Jafari Jozani
    AU  - Nima Dianati
    Y1  - 2017/01/06
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    N1  - https://doi.org/10.11648/j.avs.20160406.13
    DO  - 10.11648/j.avs.20160406.13
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    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
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    EP  - 102
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20160406.13
    AB  - This study conducted to comparison of some blood constituents between horses participated an endurance racing. Jugular vein puncture was done to collect blood from15 horses (1 gelding, 2 mares, and 12 male). These horses had been classified to three breeds include 12Kurd, 1 pure Arab, 2cross-breed and participated in the endurance racing of 0 (before competition), 15 (After first loop) and 30 km (final loop). Horses were classified in three age-groups include below 5(n=1), 5-10 (n=10) and above 10years of age (n=4). Hematocrit and Hemoglobin percentage, Total protein concentration, Red blood cell and White blood cell counting, glucose and cortisol level were measured at before starting competition (0), 15 and 30 km. Data was analyzed by SPSS (version 22). Results showed significant difference in glucose level after 30 km (P<0.05) but not after 15km (P>0.05). No significant difference was observed between cortisol concentration and age groups (P>0.05) but, there was significant difference between cortisol concentration and gender (P<0.05). Breed had influenced on HT, Hb, TP and WBC (P<0.05). Otherwise breed had no effect on RBC, glucose level and cortisol concentration (P>0.05). HT and WBC counting were higher in Arab, cross breed and Kurd respectively. Hemoglobin percentage was found higher in Arab, Kurd and Cross breed respectively. Total protein concentration in Arab was higher than Cross breed and Kurd. In conclusion, in short distance endurance competition of horses, stress is not effect on metabolism of animal, furthermore, gender and breed could important in blood parameters alterations.
    VL  - 4
    IS  - 6
    ER  - 

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