Animal and Veterinary Sciences
Volume 4, Issue 2, March 2016, Pages: 26-31
Received: May 3, 2016;
Published: May 4, 2016
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Hesham Elzorba, Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
Hossney El Banna, Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
Dina Derbala, Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
Herbal medicine is the most widely used form of medicine in the world today as it contains curative bioactive ingredients such as alkaloids, coumarins, Saponins and flavonoids. Calendula officinalis Linn. (Asteraceae) is used medicinally in Europe, China and India amongst several places in the world. It is also known as “African marigold” and has been a subject of several chemical and pharmacological studies. It is used in traditional medicine, especially for wound healing, jaundice, blood purification, and as an antispasmodic. Therefore, the aim of the present study was to explore phytochemical, toxicological and some pharmacological activities of Calendula officinalis Linn. 70% ethanolic extract. The toxicological pattern of the ethaolic extract Calendula officinalis flowers was studied by the determination of LD50 in mice by oral administration of upgrading doses 70% ethanolic extract with continuous monitoring.The oral anti-inflammatory (Formalin induced edema), antipyretic (Brewer’s yeast induced hyperthermia) and analgesic (writhing test)effects of 70% ethanolic extract of Calendula officinalis were carried out on experimental animals. The obtained results revealed that phytochemical screening of 70% ethanolic extract of Calendulsa officinalis Linn. indicates the presence of alkaloid, tannin, Saponin, Glycosides, Resin and flavonoid. The toxicological studies revealed that the minimum lethal dose of Calendula officinalis Linn. 70% ethanolic extract was found to be 2000 mg/kg body weight and LD50 of the studied extract was found to be 2450mg/Kg B.Wt. The pharmacological studies performed on Calendula officinalis Linn. ethanolic extract exhibited significant antipyretic, analgesic and Anti-inflammatory when given in a dose 250mg/Kg B.Wt.
Hossney El Banna,
Some Pharmacological & Toxicological Activities of Calendula officinalis Linn. Flower 70% Ethanolic Extract, Animal and Veterinary Sciences.
Vol. 4, No. 2,
2016, pp. 26-31.
Okigo, R. N.; Anuagasi, C .L. and Amadi, J. E. (2009): Advances in selected medicinal and aromatic plants indigenous to Africa. Journal of Medicinal Plants Research, 3 (2): 86-95. J. Clerk Maxwell, A Treatise on Electricity and Magnetism, 3rd ed., vol. 2. Oxford: Clarendon, 1892, pp. 68–73.
Block, G. (1992): The data support a role for antioxidants in reducing cancer risk. Nutrition reviews Journal, 50(7): 207-213.
Block, G. and Langseth, L. (1994): Antioxidant vitamins and disease prevention. Food Tech. 48: 80-84.R. Nicole, “Title of paper with only first word capitalized,” J. Name Stand. Abbrev.
Kartal, M. (2007): Intellectual property protection in the natural drug discovery. Traditional herbal medicine and herbal medicinal products. Phytotherapy Research Journal, 21(2): 113-119.
Guyton, A. C. and J. E. Hall, (1998): Textbook of Medical Physiology. 9th ed. W.B. Saunders Company, 920-922.
Kemper, K. J. (1999): Calendula (Calendula officinalis). The Centre for Holistic Pediatric Education and Research cited in Safdar, W.; Majeed, H.; Naveed, I.; Kayani, W. K.; Ahmed, H. ; Hussain, S. and Kamal, A.(2010): Pharmacognostical study of the Medicinal plant Calendula officinalis L. (Family Compositae). International Journal of Cell & Molecular Biology, 1(2): 108-11
Talbert, R. (2015): An Herb Society of America Guide. The Herb Society of America cited in http://www.herbsociety.org/factsheets/Calendula Guide.pdf
Zitterl-Eglseer, K.; Sosa, S.; Jurenitsch, J., Schubert-Zsilavecz, M.; Loggia, D.R.; Tubaro, A.; Bertoldi, M, Franz C. (1997): Anti-oedematous activities of the main triterpendiol esters of marigold (Calendula officinalis L.). Journal of Ethnopharmacology 57: 139–144.
Duke, J. A. (1991): Handbook of Medicinal Herbs. Boca Raton: Cancer Rresearch center Press. 87-88
Egyptian pharmacopeia (1984): Cairo organization for government printing office, 2 (3): 1158.
Evans, W. C. (1996): Trease and Evans Pharmacognosy 14th Ed., W. B. Sounders Company limited, 1545-1546.
Sofowora, A. (1993): Medicinal Plants and Traditional Medicines in Africa. The Journal of alternative and Complementary Medicine, 2(3): 365-372.
Kerber, G. (1941) Pharmakolgishe Methoden Zur Auffindung Von Arzneimitten Und Gifter Und Analyse Ihrer Wirkungweise Vor. Dr. Med Leopold Ther. Wissen schaftliche Verlage Gerlarge Geese Gesellschaft M. B. H.
Alperman, H. (1972): Bericht Uber Pharmakologische Untersuchungen Mit Fenbendazol. Abteilung Fur Pharmakologie, 863: 1-9.
Okun, R.; Liddon,S.G. and Lasagana,L. (1963): Screening methods in pharmacology. Journal of Pharmacology and Experimental Therapeutics,139: 107-114.
Domenjoz, R.; Theobald, W. and Morsdorf, K. (1955): The Effect of Anti-Inflammatory Agents on formalin edema and on the vitamin C and cholesterol content of the adrenal glands in hypophysectomized Rats. Archives internationals de pharmacodynamie et de Thérapie 103(2-3): 341-352
Roopashree, T. S.; Dang, R.; Shobha, R. H. and Narendra, C. (2008): Antibacterial Activity of Antipsoriatic Herbs: Cassia Tora, Momordica Charantia and Calendula officinalis Linn. International Journal of applied research in natural products. 1(3): 20-28
Muley, B. P.; Khadabadi, S. S. and Banarase, N. B. (2009) Phytochemical Constituents and Pharmacological activities of Calendula officinalis Linn. (Asteraceae). Tropical Journal of Pharmaceutical research. 8(5): 455-465.
Mullaicharam, A. R.; Amaresh, N. and Balasubramanian, H. (2014): Phytochemistry and Pleiotropic Pharmacological Properties of Calendula officinalis Linn. - A Review. Journal of Pharmacognosy and phytochemistry, 2 (4): 1-10.
Farahpour, M. R. (2014): Antioxidant activity, antinociceptive and anti-inflammatory effects of pot marigold hydroalcoholic extract on experimental animals. International journal of pharmtech research 6(5): 1640-1646
Kumar, A.; Singh, P. and Dora, J. (2013) Phytochemistry and Pharmacological activities of Calendula officinalis. International Journal of Inventions in Pharmaceutical Sciences, 1(1): 59-63.
Rigane, G.; Ben Younes S.; Ghazghazi, H. and Ben Salem (2013): Investigation into the biological activities and chemical composition of Calendula officinalis Linn. L. growing in Tunisia. International Food Research Journal, 20(6): 3001-3007.
Nand, P.; Drabu, S. and Gupta, R. K. (2012): Phytochemical and Antimicrobial Screening of Medicinal Plants for the treatment of acne. Indian Journal of natural Products and Resources, 3 (1): 28-32.
Leffa, D. D.; Da Rosa, R.; Munhoz, B. P.; Mello, A. D. M; Mandelli, F. D.; Amaral, P. D.; Rossatto, A. E. and De Andrade, V. M. (2012): Genotoxic and antigenotoxic properties of Calendula. Advances in life sciences 2(2): 21-28.
Buck, W.B.; Osweiter, G.D and Van Gelder, A.G. (1976): Clinical and diagnostic veterinary toxicology 2nd ed. Kendall/Hunt publishing company, Dubuque, Iowa: 69-75.
Spacer, C. B. and Breder, C. D. (1994): The neurologic basis of fever. New England Journal of Medecine. 330(26):1880-1886.
Farahmandlou, N.; Shahidi, S.; Mahmoodi, M. (2012): Effects of Calendula officinalis Linn. On pain threshold in male rats. International Conference on Chemical, Biological and Medical Sciences.
Deraedt, R.; Jouquey, S.; Delevallée, F.; Flahaut M. (1980): Release of prostaglandins E and F in an allogenic reaction and its inhibition. European Journal of Pharmacology, 61(1): 17-24.
Dewan, S.; Kumar, S. and Kumar, V. (2000): Antipyretic effect of latex of Calotropis proceara. Indian journal of Pharmacology, 32(3): 252-315.
Ahmad, S.; Qureshi, S.; Atiq-ur-Rehman and Badar, Y. (2000): Antipyretic and analgesic activity in crude ethanolic extract of Calendula officinalis Linn. Pakistan journal of scientific and industrial research, 43(1), 50-54.
Chen, Q. (1993): Methodology in pharmacological study on Chinese materia medica. 7 people‘s medical publishing house, 360.
Panthong, A.; Kanjanapothi, D.; Taesotikul, T,; Phankummoon, A.; Panthog, K.; Reutrakul, V. (2004): Anti-inflammatory activity of methanolic extracts from ventilago harmandiana pierre, Journal of Ethnopharmacology,91 (2-3): 237-242.
Preethi, K. C.; kuttan, G.; Kuttan, R. (2009): Anti-inflammatory activity of flower extract of Calendula officinalis Linn. and its possible mechanism of action. Indian journal of experimental biology. 47(2): 113-120.
Cross, C. E.; Halliwell, B.; Borish, E.T.; Pryor, W. A.; Ames, B. N. and Saul, R.L.(1987): Oxygen radicals and human diseases. Annal. Int. Med, 107(4): 526-545.
Winrow, V. R.; Winyard, P. G.; Morris, C. J.; Blake, D. R. (1993): Free radicals in inflammation:second messangers and mediators of tissue destruction. British Medical Bulletin, 49(3): 506-522.
Parke, D. V.; Sapota, A. (1996): Chemical toxicity and reactive oxygen species. International Journal of Occupational Medicine and Environmental Health, 9(4): 331–340
Khalid, K. A. and Da Silva, J.A.T. (2012): Biology of Calendula officinalis Linn. Focus On Pharmacology, Biological Activities and Agronomic Practices. Medicinal and Aromatic Plant Science and Biotechnology.6(1):12-27
Singh, M. Kr.; Sahu, P.; Nagori K.; Dewangan, D., Kumar, T.; Alexander, A.; Badwaik, H. and Tripathi, D. K. (2011): Organoleptic Properties In-Vitro and In-Vivo Pharmacological Activities of Calendula officinalis Linn. Journal of Chemical and Pharmaceutical Research, 3(4): 655-663.
Vane, J. R. (1971): Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nature new biology, 231 (25): 232-5.