Animal and Veterinary Sciences

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Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors

Received: 18 October 2014    Accepted: 03 November 2014    Published: 28 February 2015
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Abstract

Growth and propagation of infectious bursal disease virus (IBDV) in chicken embryo is time consuming and costly, and an appropriate serological test is required to detect and identify IBDV strains, therefore, a suitable cell lines in which different IBDV strains can grow well has been needed. The aim of the present work was to study the growth of different IBDV strains in cell lines from lymphoid leukosis tumors using histopathological staining, indirect immunofluorescence, immunohistochemistry and transmission electron microscopic examination. In conclusion, cell lines from origin of lymphoid leukosis tumors; LSCC-BK3 and LSCC-CU10 are suitable for growth and propagation of different IBDV strains. IBDV strains resulted into histopathological lesions of variable severity differ according to the patho-type of IBDV and time after virus inoculation. Indirect immunofluorescent test could be used for detection and differentiation of IBDV strains inoculated into cell lines from origin of lymphoid leukosis tumors. Trials of immunohistochemistry technique for detection of different IBDV strains in cell lines, were unsuccessful. Using transmission electron microscopy, IBDV particles could be detected in all infected cell lines.

DOI 10.11648/j.avs.20150302.13
Published in Animal and Veterinary Sciences (Volume 3, Issue 2, March 2015)
Page(s) 46-50
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Infectious Bursal Disease Virus, Cell Lines, Histopathological Staining, Immunohistochemistry, Immunofluorescence

References
[1] Calnek, BW, Murthy, KK and Schat, KA. Establishment of Marek's disease lymphoblastoid cell lines from transplantable versus primary lymphomas. Int J Cancer 1978; 21: 100-107.
[2] Cosgrove, AS. An apparently new disease of chickens – avian nephrosis. Avian Dis 1962; 6: 385-389.
[3] Eterradossi, N and Saif, YM. Infectious Bursal Disease. In Saif, YM, Fadly, AM , Glisson, JR, McDougald, LR, Nolan, LK and Swayne, DE, editors. Diseases of Poultry, 12th ed, Ames, Iowa, USA: Blackwell Publishing Professional; 2008, p. 185-208.
[4] Goryo, M. Experimental study of very virulent infectious bursal disease virus (vvIBDV). Avian Dis 1992; 36: 13-22.
[5] Hihara, H, Yamamato, H, Arai, K, Okazaki, W and Shimizu, T. Conditions for successful cultivation of tumor cells from chickens with avian lymphoid leukosis. Avian Dis 1980; 24: 971:979.
[6] Hirai, K and Calnek, BW. In vitro replication of infectious bursal disease virus in established lymphoid cell lines and chicken B lymphocytes. Infect Immun 1979; 25: 964-970.
[7] Hirai, K. and Shimakura, S. Structure of infectious bursal disease virus. J Virol 1974; 14: 957-964.
[8] Hitchner, SB. Infectivity of infectious bursal disease virus for embryonating eggs. Poult Sci 1970; 49: 511-516.
[9] Hussain, I, Rasool, MH and Mahmood, MS. Production of hyperimmune serum against infectious bursal disease virus in rabbits. Pak Vet J 2004; 24: 179-183.
[10] Jackwood, DH, Saif, YM and Hughes, JH. Replication of infectious bursal disease virus in continuous cell lines. Avian Dis 1987; 31: 370-375.
[11] Kibenge, FSB, Dhillon, AS and Russell, RG. Growth of serotypes I and II and variant strains of infectious bursal disease virus in vero cells. Avian Dis 1988; 17: 298-303.
[12] Kibenge, FSB, Dhillon, AS and Russell, RG. Biochemistry and immunology of infectious bursal disease virus. J Gen Virol 1988; 69: 1757-1775.
[13] Kibenge, FSB, McKenna, PK and Dybing, JK. Genome cloning and analysis of the large RNA segment (segment A) of a naturally avirulent serotype 2 infectious bursal disease virus. Virology 1991; 184: 437-40.
[14] Lee, LH and Lukert, PD. Adaptation and antigenic variation of infectious bursal disease virus. J Chin Soci Vet Sci 1986; 12: 297-304.
[15] Lukert, PD. and Davis, RB. Infectious bursal disease virus: growth and characterization in cell cultures. Avian Dis 1974; 18: 243-250.
[16] Lukert, PD and Saif, YM. Infectious bursal disease virus. In: Calnek, BW, Barnes, HJ, Beard, CW, Mc Dougald, LR and Saif, YM, editors. Diseases of Poultry, 10th ed. Ames, Iowa, USA: Iowa State University Press; 1997, p.721-738.
[17] Lukert, PD, Leonard, J and Davis, RB. Infectious bursal disease virus: Antigen production and immunity. Am J Vet Res 1975; 36: 539-540.
[18] MacDonald, RD. Immunofluorescent detection of double-stranded RNA in cells infected with reovirus, infectious pancreatic necrosis virus, and infectious bursal disease virus. Can J Microbiol 1980; 26: 256-261.
[19] McFerran, JB, McNutly, M, McKilliop, ER, Conner, TJ, McCracken, RM, Collins, DS and Allan, GM. Isolation and serological studies with infectious bursal disease virus from fowl, turkeys and ducks: demonstration of a second serotype. Avian Pathol 1980; 9: 395-404.
[20] McNulty, MS, Allan, GM and Mc Ferran, JB. Isolation of infectious bursal disease virus from turkeys. Avian Pathol 1979; 8: 205-212.
[21] Muller, H, Islam, MR and Raue, R. Research on infectious bursal disease – The past, the present, and the future. Vet Microbiol 2003; 97:153-165.
[22] Muller, H, Lange, H and Becht, H. Formation, characterization and interfering capacity of a small plaque mutant and of incomplete virus particles of infectious bursal disease virus. Virus Res 1986; 4: 297-309.
[23] Petek, M, D'Aprile, PN and Cancellotti, F. Biological and physicochemical properties of the infectious bursal disease virus (IBDV). Avian Pathol 1973; 2: 135-152.
[24] Rinaldi, A, Lodetti, E, Cessi, D, Lodrini, E, Cervio, G and Nardelli, L. Coltura del virus de Gumboro (IBA) su fibroblast de embrioni di pollo. Nuova Vet 1972; 48: 195-201.
[25] Rosenberger, JK, Saif, YM and Jackwood, DJ. Infectious Bursal Disease. In: Dufour-Zavala, L, Swayne, DE, Glisson, J. RJ, Pearson, E, Reed, WM, Jackwood, MW and Woolcock, PR, editors. A laboratory manual for the isolation and identification of avian pathogens 5th ed, American Association of Avian Pathologists; 2008 p. 188-190.
[26] Roy, P, Raja, A and Dhillon, AS. Counterimmunoelectrophoresis for diagnosis of infectious bursal disease: comparative efficacy of three different electrophoresis buffers. J. Appl Poult Res 2008; 17: 116-120.
[27] Skeeles, JK and Lukert, PD. Studies with an attenuated cell-culture-adapted infectious bursal disease virus: Replication sites and persistence of the virus in specific-pathogen-free chickens. Avian Dis 1980; 24: 43-47.
[28] Tsukamoto, K, Matsumura, T, Mase, M. and lmai, K. A highly sensitive, broad-spectrum infectivity assay for infectious bursal disease virus. Avian Dis 1995; 39: 575-586.
[29] Van den Berg, TP. Acute infectious bursal disease in poultry: a review. Avian Pathol 2000; 29: 175-194.
[30] Vieltiz, E. Aspects of Protection against Gumboro virus. Zoo International 1993; 16: 42-49.
[31] Yamaguchi, S, Imada, I and Kawamura, H. Growth and infectivity titration of virulent infectious bursal disease virus in established cell lines from lymphoid leukosis. Avian Dis 1981; 25: 927-935.
Author Information
  • Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka, 020-8550, Japan; Department of Poultry diseases, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt

  • Department of Poultry diseases, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt

  • Department of Poultry diseases, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt

  • Department of Poultry diseases, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt

  • Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka, 020-8550, Japan

  • Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka, 020-8550, Japan

Cite This Article
  • APA Style

    Ahmed Hassan, Mostafa Shahata, Elrefaie Refaie, Ragab Ibrahim, Jun Sasaki, et al. (2015). Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors. Animal and Veterinary Sciences, 3(2), 46-50. https://doi.org/10.11648/j.avs.20150302.13

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    ACS Style

    Ahmed Hassan; Mostafa Shahata; Elrefaie Refaie; Ragab Ibrahim; Jun Sasaki, et al. Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors. Anim. Vet. Sci. 2015, 3(2), 46-50. doi: 10.11648/j.avs.20150302.13

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    AMA Style

    Ahmed Hassan, Mostafa Shahata, Elrefaie Refaie, Ragab Ibrahim, Jun Sasaki, et al. Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors. Anim Vet Sci. 2015;3(2):46-50. doi: 10.11648/j.avs.20150302.13

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  • @article{10.11648/j.avs.20150302.13,
      author = {Ahmed Hassan and Mostafa Shahata and Elrefaie Refaie and Ragab Ibrahim and Jun Sasaki and Masanubu Goryo},
      title = {Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors},
      journal = {Animal and Veterinary Sciences},
      volume = {3},
      number = {2},
      pages = {46-50},
      doi = {10.11648/j.avs.20150302.13},
      url = {https://doi.org/10.11648/j.avs.20150302.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.avs.20150302.13},
      abstract = {Growth and propagation of infectious bursal disease virus (IBDV) in chicken embryo is time consuming and costly, and an appropriate serological test is required to detect and identify IBDV strains, therefore, a suitable cell lines in which different IBDV strains can grow well has been needed. The aim of the present work was to study the growth of different IBDV strains in cell lines from lymphoid leukosis tumors using histopathological staining, indirect immunofluorescence, immunohistochemistry and transmission electron microscopic examination. In conclusion, cell lines from origin of lymphoid leukosis tumors; LSCC-BK3 and LSCC-CU10 are suitable for growth and propagation of different IBDV strains. IBDV strains resulted into histopathological lesions of variable severity differ according to the patho-type of IBDV and time after virus inoculation. Indirect immunofluorescent test could be used for detection and differentiation of IBDV strains inoculated into cell lines from origin of lymphoid leukosis tumors. Trials of immunohistochemistry technique for detection of different IBDV strains in cell lines, were unsuccessful. Using transmission electron microscopy, IBDV particles could be detected in all infected cell lines.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors
    AU  - Ahmed Hassan
    AU  - Mostafa Shahata
    AU  - Elrefaie Refaie
    AU  - Ragab Ibrahim
    AU  - Jun Sasaki
    AU  - Masanubu Goryo
    Y1  - 2015/02/28
    PY  - 2015
    N1  - https://doi.org/10.11648/j.avs.20150302.13
    DO  - 10.11648/j.avs.20150302.13
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
    SP  - 46
    EP  - 50
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20150302.13
    AB  - Growth and propagation of infectious bursal disease virus (IBDV) in chicken embryo is time consuming and costly, and an appropriate serological test is required to detect and identify IBDV strains, therefore, a suitable cell lines in which different IBDV strains can grow well has been needed. The aim of the present work was to study the growth of different IBDV strains in cell lines from lymphoid leukosis tumors using histopathological staining, indirect immunofluorescence, immunohistochemistry and transmission electron microscopic examination. In conclusion, cell lines from origin of lymphoid leukosis tumors; LSCC-BK3 and LSCC-CU10 are suitable for growth and propagation of different IBDV strains. IBDV strains resulted into histopathological lesions of variable severity differ according to the patho-type of IBDV and time after virus inoculation. Indirect immunofluorescent test could be used for detection and differentiation of IBDV strains inoculated into cell lines from origin of lymphoid leukosis tumors. Trials of immunohistochemistry technique for detection of different IBDV strains in cell lines, were unsuccessful. Using transmission electron microscopy, IBDV particles could be detected in all infected cell lines.
    VL  - 3
    IS  - 2
    ER  - 

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