Diet-Dependent Rumen Epithelial NHE1 and NHE3 Expression in Sheep
Animal and Veterinary Sciences
Volume 2, Issue 6, November 2014, Pages: 208-212
Received: Dec. 3, 2014; Accepted: Dec. 11, 2014; Published: Dec. 19, 2014
Views 2786      Downloads 117
Authors
Rasha S. Ahmed, Department of Anatomy, Faculty of Veterinary Medicine, P.O. Box 32, University of Khartoum, Sudan, Cell Phone 00249916289454
Holger Martens, Institut of Physiology, Faculty of Veterinary Medicine (WE02), Oertzenweg 19b, Hs. 11 Raum 1.49, Haus B (rechts) 1. Stock 14163 Berlin
Christoph Muelling, Institut of Anatomy, Faculty of Veterinary Medicine, An den Tierkliniken 43,D-04103 Leipzig, Germany
Article Tools
Follow on us
Abstract
The objective of this study was to characterize the immunohistochemical localization of Na+/H+ exchanger isoforms (NHE1 and NHE3) in the rumen epithelium of Sheep after changing the diet from hay (ad libitum) to a mixed hay/concentrate diet. A total of 24 sheep were fed mixed hay/concentrate for different periods ranging from 0 weeks (control; hay ad libitum) to 12 weeks (1-1.5 kg hay plus 780 g concentrate per day in two equal portions). NHE3-immunostaining was found to be more intense at both stratum granulosum (deep layer) and stratum spinosum (superficial layer), with decreasing intensity through stratum spinosum (deep or suprabasal layer) and stratum basal. Stratum corneum was negative. Distribution of NHE3 isoform was different within the different strata. In stratum granulosum and stratum spinosum (superficial layer), NHE3 isoform was distributed predominant at the apical surface /membrane of the cells. Meanwhile, in both stratum spinosum (deep layer) and stratum basale, intracellular NHE3 isoform was predominantly. The degree of antibody reaction was weak in hay-fed sheep and in all concentrate-fed groups, except in 2 and 4 weeks concentrate-fed groups, in which the degree of the antibody reaction was moderate and strong, respectively. NHE1 isoform was not detected in the sheep-rumen epithelium.
Keywords
Sheep, Rumen Epithelium, NHE1, NHE3, Feed
To cite this article
Rasha S. Ahmed, Holger Martens, Christoph Muelling, Diet-Dependent Rumen Epithelial NHE1 and NHE3 Expression in Sheep, Animal and Veterinary Sciences. Vol. 2, No. 6, 2014, pp. 208-212. doi: 10.11648/j.avs.20140206.18
References
[1]
Biemesderfer, D.; Rutherford, P. A.; Nagy, T.; Pizzonia, J. H.; Abu-Alfa, A. K. and Aronson, P. S. (1997) Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney. Am. J. Physiol. 273: F289-F299
[2]
Chow, C. W.; Khurana, S.; Woodside, M.; Grinstein, S. and Orlowski, J. (1999) The epithelial Na+/H+ exchanger, NHE3, is internalized through a clathrin-mediated pathway.J. Biol. Chem. 274: 37551-37558
[3]
De Silva, M. G.; Elliott, K.; Dahl, H. H.; Fitzpatrick, E. and Wilcox, S. (2003) Disruption of a novel member of a NHE family and DOCK3 is associated with an attention deficit hyperactivity disorder-like phenotype. J. Med. Genet. 40: 733-740.
[4]
Etschmann, B, Suplie, A and Martens, H (2009): Change of ruminal sodium transport in sheep during dietary adaptation. Archives of Animal Nutrition, 63: 26-38.
[5]
Gäbel, G.; Martens, H.; Suendermann, M. and Galfi, P. (1987) The effect of diet, intraruminal pH and osmolarity on sodium, chloride and magnesium absorption from the temporarily isolated and washed reticulo-rumen of sheep. Q. J. Exp. Physiol. 72: 501-511
[6]
Gäbel, G.; Vogler, S. and Martens, H. (1991) Short chain fatty acids and CO2 as regulators of Na+ and Cl- absorption in isolated sheep rumen mucosa. J. Comp. Physiol. B, 161: 419-426
[7]
Goyal, S.; Vanden, H. G. and Aronson, P. S. (2003) Renal expression of novel Na+/H+ exchanger isoform NHE8. Am. J. Physiol. 284: F467-F473
[8]
Graham, C.; Gatherar, I.; Haslam, I.; Glanville, M. and Simmons, N. L. (2007) Expression and localization of monocarboxylate transporters and sodium/proton exchangers in bovine rumen epithelium. Am. J. Physiol. Regulatory Integrative Comp. Physiol. 292: 997-1007
[9]
Grinstein, S.; Clarke, C. A. and Rothstein, A. (1988) Activation of Na+/H+ exchange in lymphocytes by osmatically induced volume changes and by cytoplasmatic acidification. J. Gen. Physiol. 82: 619-638
[10]
Hoogerwerf, W. A.; Tsao, Su. C.; Devuyst, O.; Levine, S. A.; Chris Yun, C. H.; Yip, J. W.; Cohen, M. E.; Wilson, P. D.; Lazenby, A. J.; Tse, C. M. and Donowitz, M. (1996) NHE2 and NHE3 are human and rabbit intestinal brush-border proteins. Am. J. Physiol. 270: G29-G41
[11]
Kiela, P. R.; Kuscuoglu, N.; Midura, A. J.; Midura-Kiela, M. T.; Larmonier, C. B.; Lipko, M.; Ghishan, F. K. (2007) Molecular mechanism of Rat NHE3 gene promoter regulation by sodium butyrate. Am. J. Physiol., cell physiol. 293: C64-74
[12]
Kurashima, K.; Szabo, E. Z.; Lukacs, G.; Orlowski, J. and Grinstein, S. (1998) Endosomal recycling of the Na+/H+ exchanger NHE3 isoform is regulated by the phosphatidylinositol 3-kinase pathway. J. Biol. Chem. 273: 20828-20836
[13]
Lang, F.; Busch, G. L.; Ritter, M.; Völkl, H.; Waldegger, S.; Gulbins, E. and Häussinger, D. (1998) Functional significance of cell volume regulatory mechanisms. Physiol. Rev. 78: 247-306
[14]
Masereel, B.; Pochet, L. and Laeckmann, D. (2003) An overview of inhibitors of Na+/H+ exchanger. Eur. J. Med. Chem. 38: 547-554
[15]
Noel, J. and Pouyssegur, J. (1995) Hormonal regulation, Pharmacology, and membrane sorting of vertebrate Na+/H+ exchanger isoform. Am. J. Physiol. 268: C 283-C296
[16]
Putney, L. K.; Denker, S. P. and Barber, D. L. (2002) The changing face of the Na+/H+ exchanger, NHE1: structure, regulation, and cellular actions. Annu. Rev. Pharmacol. Toxicol. 42: 527-552
[17]
Rabbani, I; Siegling-Vlitakis, C; Noci, B; Martens, H. (2011): Evidence for NHE3-mediated Na transport in sheep and bovine forestomach. Am J Physiol Regul Integr Comp Physiol. 30: 313-319.
[18]
Ritter, M.; Fuerst, J.; Woll, E.; Chwatal, S.; Gechwentner, M.; Lang, F. and Paulmichl, M. (2001) Na+/ H+ exchangers; linking osmotic dysequilibrium modified cell function. Cell Physiol. Biochem. 11: 1-18.
[19]
Romeis, B. (1989) Mikroskopische Technik. Verlag Urban und Schwarzenberg, München, Wien und Baltimore, 17. Auflage.
[20]
Schweigel, M.; Freyer, M.; Leclercq, S.; Etschmann, B.; Lodemann, U.; Bottcher, A. and Martens H. (2005) Luminal hyperosmolarity decreases Na transport and impairs barrier function of sheep rumen epithelium. J. Comp. Physiology. B, 175: 575-591.
[21]
Sehested, J.; Diernaes, I.; Moller, P. D. and Skadhauge, E. (1996) Transport of Na across the isolated bovine rumen epithelium: Interaction with short-chain fatty acids, chloride and bicarbonate. Exp. Physiol. 81: 79-94.
[22]
Wakabayashi, S.; Shigekawa, M. and Pouyssegur, J. (1997) Molecular physiology of vertebrate Na+/H+ exchangers. Physiol. Rev. 77: 51-74.
[23]
Yun, C. H.; Tse, C. M.; Nath, S. K.; Levine, S. A.; Brant, S. R. and Donowitz, M. (1995a) Mammalian Na_/H_ exchanger gene family: structure and function studies. Am. J. Physiol. Gastrointest. Liver Physiol. 269: G1–G11
[24]
Yun, C.; Tse, C.; Levine, S. and Donowitz, M. (1995b) Structure/function studies of mammalian Na+/ H+ exchangers – an update. J. Physiol. 482: 1-6.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186