Morphological Plasticity of Ticks’ Salivary Glands and the Meaning of Hematophagy in Hosts Immunized with Glandular Extract of Females Fed for 4 Days
Animal and Veterinary Sciences
Volume 2, Issue 6, November 2014, Pages: 194-207
Received: Nov. 11, 2014;
Accepted: Nov. 28, 2014;
Published: Dec. 15, 2014
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Karim Christina Scopinho Furquim, Departamento de Biologia, Instituto de Biociências, UNESP, Av. 24 A, nº 1515, Cx. Postal 199, CEP: 13506-900, Rio Claro, S.P., Brazil
Maria Izabel Camargo Mathias, Departamento de Biologia, Instituto de Biociências, UNESP, Av. 24 A, nº 1515, Cx. Postal 199, CEP: 13506-900, Rio Claro, S.P., Brazil
Gislaine Cristina Roma, Departamento de Biologia, Instituto de Biociências, UNESP, Av. 24 A, nº 1515, Cx. Postal 199, CEP: 13506-900, Rio Claro, S.P., Brazil
Letícia Maria Gráballos Ferraz Hebling, Departamento de Biologia, Instituto de Biociências, UNESP, Av. 24 A, nº 1515, Cx. Postal 199, CEP: 13506-900, Rio Claro, S.P., Brazil
Gervásio Henrique Bechara, Departamento de Patologia Veterinária, FCAV, UNESP, Via de Acesso Prof. Paulo Castellane, s/n, CEP: 14884-900, Jaboticabal, S.P., Brazil
A histochemical analysis was performed in this study in order to detect proteins, polysaccharides, lipids, acid phosphatase and calcium in the secretion produced by the salivary glands of Rhipicephalus sanguineus females (Latreille, 1806) (Acari, Ixodidae) fed for 2, 4 and 6 days in New Zealand White rabbits which had been previously immunized with glandular extract obtained from females fed for 4 days (SGE4). The results revealed that such glands presented alterations in their secretory cycle, which occurred: a) by the inactivity of some c1 cells (in the glands of females fed for 2 days) and c4 (in those fed for 4 days) and b) by the modification in the constitution of the secretion of females fed for 2-6 days. It was verified that, in the glands of females fed for 2 days, there was an increase in proteins and calcium; a reduction in lipids and the contents of polysaccharides and acid phosphatase remained unaltered. In those fed for 4 days there was an increase in proteins, calcium and acid phosphatase; reduction in the lipids, and the content of polysaccharides remained unaltered. In the females fed for 6 days an increase in the components was observed; however, there was a reduction in lipids and acid phosphatase. In addition, it was verified that, in a decrescent order of histochemical alterations, the most affected cells were: f; c2, c3, c5; a, d and c4, e in the glands of females fed for 2 days; c5; a, c2, c3, d and c1, e in the glands of females fed for 4 days and a, c1, c2, d and e, c3 in the glands of females fed for 6 days. The data here obtained clearly show that the most pronounced histochemical modifications were detected in the glands of females fed for 2 and 6 days; however, the modifications observed in the females fed for 4 days must also be considered, once they were significant as well. This has probably occurred because the hosts were inoculated with SGE4, obtained from the salivary glands of females fed for 4 days, intermediate stage of the glandular cycle, which contains antigens that are common to glandular tissues of females fed for 2 and 6 days.
Karim Christina Scopinho Furquim,
Maria Izabel Camargo Mathias,
Gislaine Cristina Roma,
Letícia Maria Gráballos Ferraz Hebling,
Gervásio Henrique Bechara,
Morphological Plasticity of Ticks’ Salivary Glands and the Meaning of Hematophagy in Hosts Immunized with Glandular Extract of Females Fed for 4 Days, Animal and Veterinary Sciences.
Vol. 2, No. 6,
2014, pp. 194-207.
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