Animal and Veterinary Sciences

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Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus

Received: 27 August 2014    Accepted: 05 September 2014    Published: 20 September 2014
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Abstract

Chemical extract of Nerium oleander (NO) is identified as in vitro antibacterial and antifungal activities. However, there is no any study about antiviral effect of NO distillate (NOD). The aim of this study was to evaluate in vitro effects of NOD alone and combined with acyclovir on Parainfluenza-3 (PI-3) virus. Lyophilized NOD was dissolved at concentration of 10 mg/mL with distillated water and filtered. The thiazolyl blue test was used to evaluate the cytotoxic activity of the NOD, and acyclovir. NOD did not cause cytotoxicity against to MDBK cells up to 10 mg/mL concentrations while acyclovir did not cause cytotoxicity up to 0.97 µg/mL. NOD (10 mg/mL), acyclovir (0.97 µg/mL) and NOD + acyclovir were treated with 100 TCID50 diluted PI-3 in 96-well plates. Antiviral activity of NOD was statistically (P<0.05) different from NOD + acyclovir against PI-3. The most effective results obtained from using single of NOD (P<0.05) while acyclovir was not effective (P>0.05). In conclusion, it is referred that NOD has antiviral effect and it can be evaluated in the treatment of PI-3 in future.

DOI 10.11648/j.avs.20140205.14
Published in Animal and Veterinary Sciences (Volume 2, Issue 5, September 2014)
Page(s) 150-153
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

PI-3, Nerium Oleander, Acyclovir, Antiviral

References
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Author Information
  • Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, Konya, Turkey

  • Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Selcuk, Konya, Turkey

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  • APA Style

    Oguzhan Avci, Burak Dik. (2014). Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus. Animal and Veterinary Sciences, 2(5), 150-153. https://doi.org/10.11648/j.avs.20140205.14

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    Oguzhan Avci; Burak Dik. Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus. Anim. Vet. Sci. 2014, 2(5), 150-153. doi: 10.11648/j.avs.20140205.14

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    AMA Style

    Oguzhan Avci, Burak Dik. Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus. Anim Vet Sci. 2014;2(5):150-153. doi: 10.11648/j.avs.20140205.14

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  • @article{10.11648/j.avs.20140205.14,
      author = {Oguzhan Avci and Burak Dik},
      title = {Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus},
      journal = {Animal and Veterinary Sciences},
      volume = {2},
      number = {5},
      pages = {150-153},
      doi = {10.11648/j.avs.20140205.14},
      url = {https://doi.org/10.11648/j.avs.20140205.14},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.avs.20140205.14},
      abstract = {Chemical extract of Nerium oleander (NO) is identified as in vitro antibacterial and antifungal activities. However, there is no any study about antiviral effect of NO distillate (NOD). The aim of this study was to evaluate in vitro effects of NOD alone and combined with acyclovir on Parainfluenza-3 (PI-3) virus. Lyophilized NOD was dissolved at concentration of 10 mg/mL with distillated water and filtered. The thiazolyl blue test was used to evaluate the cytotoxic activity of the NOD, and acyclovir. NOD did not cause cytotoxicity against to MDBK cells up to 10 mg/mL concentrations while acyclovir did not cause cytotoxicity up to 0.97 µg/mL. NOD (10 mg/mL), acyclovir (0.97 µg/mL) and NOD + acyclovir were treated with 100 TCID50 diluted PI-3 in 96-well plates. Antiviral activity of NOD was statistically (P0.05). In conclusion, it is referred that NOD has antiviral effect and it can be evaluated in the treatment of PI-3 in future.},
     year = {2014}
    }
    

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    T1  - Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus
    AU  - Oguzhan Avci
    AU  - Burak Dik
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    N1  - https://doi.org/10.11648/j.avs.20140205.14
    DO  - 10.11648/j.avs.20140205.14
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    AB  - Chemical extract of Nerium oleander (NO) is identified as in vitro antibacterial and antifungal activities. However, there is no any study about antiviral effect of NO distillate (NOD). The aim of this study was to evaluate in vitro effects of NOD alone and combined with acyclovir on Parainfluenza-3 (PI-3) virus. Lyophilized NOD was dissolved at concentration of 10 mg/mL with distillated water and filtered. The thiazolyl blue test was used to evaluate the cytotoxic activity of the NOD, and acyclovir. NOD did not cause cytotoxicity against to MDBK cells up to 10 mg/mL concentrations while acyclovir did not cause cytotoxicity up to 0.97 µg/mL. NOD (10 mg/mL), acyclovir (0.97 µg/mL) and NOD + acyclovir were treated with 100 TCID50 diluted PI-3 in 96-well plates. Antiviral activity of NOD was statistically (P0.05). In conclusion, it is referred that NOD has antiviral effect and it can be evaluated in the treatment of PI-3 in future.
    VL  - 2
    IS  - 5
    ER  - 

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