Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis

Sibel Yavru; Oguzhan Avci; Mehmet Kale

doi:doi:10.11648/j.avs.20140205.12

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Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis

Sibel Yavru, Oguzhan Avci, Mehmet Kale

Published in Animal and Veterinary Sciences (Volume 2, Issue 5)

Received: 13 August 2014 Accepted: 20 August 2014 Published: 30 August 2014

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Abstract

The purpose of the present study was to evaluate the possible effects of Bovine Herpes virus-1 (BHV-1), Bovine Viral Diarrhea Virus (BVDV), and Bovine Herpes virus-4 (BHV-4) involving metritis in the selected unvaccinated dairy cattle herds in Afyon province of Turkey by serologically and virologically methods. A total of 63 dairy cattle with metritis were sampled in order to investigate the presence of BHV-1, BVDV and BHV-4 infections. The sera samples were tested for presence of antibodies to BHV-1, BVDV and BHV-4 using a commercially available indirect Enzyme Linked Immunosorbent Assay. Leukocyte samples were tested for presence of BVDV viral genome using Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR) for BHV-1 and BHV-4 viral genome. Detectable antibodies were detected in 6 (9.52%) of 63 against BVDV and 51 (80.95%) of 63 against BHV-4. Detectable antibodies were detected against BVDV from 6 (9.52%) of 63 sera samples and BHV-4 from 51 (80.95%) of 63. No antibodies against BHV-1 were detected as well as the results of RT-PCR for BVDV, and PCR for BHV-1 were all negative. Positive PCR results found BHV-4 genome from 8 (12.69%) of 63 leukocyte samples. Presence of BVDV and BHV-4 antibodies in unvaccinated animals indicates that these cattle had contracted infection. In conclusion, BHV-4 infections may play a direct or indirect role in causing bovine metritis; therefore their importance in the etiology of metritis and their economic impact needs further attention.

Published in	Animal and Veterinary Sciences (Volume 2, Issue 5)
DOI	10.11648/j.avs.20140205.12
Page(s)	142-145
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

BHV-1, BVDV, BHV-4, ELISA, PCR

References

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APA Style

Sibel Yavru, Oguzhan Avci, Mehmet Kale. (2014). Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis. Animal and Veterinary Sciences, 2(5), 142-145. https://doi.org/10.11648/j.avs.20140205.12

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ACS Style

Sibel Yavru; Oguzhan Avci; Mehmet Kale. Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis. Anim. Vet. Sci. 2014, 2(5), 142-145. doi: 10.11648/j.avs.20140205.12

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AMA Style

Sibel Yavru, Oguzhan Avci, Mehmet Kale. Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis. Anim Vet Sci. 2014;2(5):142-145. doi: 10.11648/j.avs.20140205.12

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@article{10.11648/j.avs.20140205.12,
  author = {Sibel Yavru and Oguzhan Avci and Mehmet Kale},
  title = {Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis},
  journal = {Animal and Veterinary Sciences},
  volume = {2},
  number = {5},
  pages = {142-145},
  doi = {10.11648/j.avs.20140205.12},
  url = {https://doi.org/10.11648/j.avs.20140205.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20140205.12},
  abstract = {The purpose of the present study was to evaluate the possible effects of Bovine Herpes virus-1 (BHV-1), Bovine Viral Diarrhea Virus (BVDV), and Bovine Herpes virus-4 (BHV-4) involving metritis in the selected unvaccinated dairy cattle herds in Afyon province of Turkey by serologically and virologically methods. A total of 63 dairy cattle with metritis were sampled in order to investigate the presence of BHV-1, BVDV and BHV-4 infections. The sera samples were tested for presence of antibodies to BHV-1, BVDV and BHV-4 using a commercially available indirect Enzyme Linked Immunosorbent Assay. Leukocyte samples were tested for presence of BVDV viral genome using Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR) for BHV-1 and BHV-4 viral genome. Detectable antibodies were detected in 6 (9.52%) of 63 against BVDV and 51 (80.95%) of 63 against BHV-4. Detectable antibodies were detected against BVDV from 6 (9.52%) of 63 sera samples and BHV-4 from 51 (80.95%) of 63. No antibodies against BHV-1 were detected as well as the results of RT-PCR for BVDV, and PCR for BHV-1 were all negative. Positive PCR results found BHV-4 genome from 8 (12.69%) of 63 leukocyte samples. Presence of BVDV and BHV-4 antibodies in unvaccinated animals indicates that these cattle had contracted infection. In conclusion, BHV-4 infections may play a direct or indirect role in causing bovine metritis; therefore their importance in the etiology of metritis and their economic impact needs further attention.},
 year = {2014}
}

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TY  - JOUR
T1  - Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis
AU  - Sibel Yavru
AU  - Oguzhan Avci
AU  - Mehmet Kale
Y1  - 2014/08/30
PY  - 2014
N1  - https://doi.org/10.11648/j.avs.20140205.12
DO  - 10.11648/j.avs.20140205.12
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 142
EP  - 145
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20140205.12
AB  - The purpose of the present study was to evaluate the possible effects of Bovine Herpes virus-1 (BHV-1), Bovine Viral Diarrhea Virus (BVDV), and Bovine Herpes virus-4 (BHV-4) involving metritis in the selected unvaccinated dairy cattle herds in Afyon province of Turkey by serologically and virologically methods. A total of 63 dairy cattle with metritis were sampled in order to investigate the presence of BHV-1, BVDV and BHV-4 infections. The sera samples were tested for presence of antibodies to BHV-1, BVDV and BHV-4 using a commercially available indirect Enzyme Linked Immunosorbent Assay. Leukocyte samples were tested for presence of BVDV viral genome using Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR) for BHV-1 and BHV-4 viral genome. Detectable antibodies were detected in 6 (9.52%) of 63 against BVDV and 51 (80.95%) of 63 against BHV-4. Detectable antibodies were detected against BVDV from 6 (9.52%) of 63 sera samples and BHV-4 from 51 (80.95%) of 63. No antibodies against BHV-1 were detected as well as the results of RT-PCR for BVDV, and PCR for BHV-1 were all negative. Positive PCR results found BHV-4 genome from 8 (12.69%) of 63 leukocyte samples. Presence of BVDV and BHV-4 antibodies in unvaccinated animals indicates that these cattle had contracted infection. In conclusion, BHV-4 infections may play a direct or indirect role in causing bovine metritis; therefore their importance in the etiology of metritis and their economic impact needs further attention.
VL  - 2
IS  - 5
ER  -

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Author Information

Sibel Yavru

Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, Turkey
Oguzhan Avci

Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, Turkey
Mehmet Kale

Department of Virology, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Burdur, 15100, Turkey

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APA Style

Sibel Yavru, Oguzhan Avci, Mehmet Kale. (2014). Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis. Animal and Veterinary Sciences, 2(5), 142-145. https://doi.org/10.11648/j.avs.20140205.12

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ACS Style

Sibel Yavru; Oguzhan Avci; Mehmet Kale. Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis. Anim. Vet. Sci. 2014, 2(5), 142-145. doi: 10.11648/j.avs.20140205.12

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AMA Style

Sibel Yavru, Oguzhan Avci, Mehmet Kale. Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis. Anim Vet Sci. 2014;2(5):142-145. doi: 10.11648/j.avs.20140205.12

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@article{10.11648/j.avs.20140205.12,
  author = {Sibel Yavru and Oguzhan Avci and Mehmet Kale},
  title = {Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis},
  journal = {Animal and Veterinary Sciences},
  volume = {2},
  number = {5},
  pages = {142-145},
  doi = {10.11648/j.avs.20140205.12},
  url = {https://doi.org/10.11648/j.avs.20140205.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20140205.12},
  abstract = {The purpose of the present study was to evaluate the possible effects of Bovine Herpes virus-1 (BHV-1), Bovine Viral Diarrhea Virus (BVDV), and Bovine Herpes virus-4 (BHV-4) involving metritis in the selected unvaccinated dairy cattle herds in Afyon province of Turkey by serologically and virologically methods. A total of 63 dairy cattle with metritis were sampled in order to investigate the presence of BHV-1, BVDV and BHV-4 infections. The sera samples were tested for presence of antibodies to BHV-1, BVDV and BHV-4 using a commercially available indirect Enzyme Linked Immunosorbent Assay. Leukocyte samples were tested for presence of BVDV viral genome using Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR) for BHV-1 and BHV-4 viral genome. Detectable antibodies were detected in 6 (9.52%) of 63 against BVDV and 51 (80.95%) of 63 against BHV-4. Detectable antibodies were detected against BVDV from 6 (9.52%) of 63 sera samples and BHV-4 from 51 (80.95%) of 63. No antibodies against BHV-1 were detected as well as the results of RT-PCR for BVDV, and PCR for BHV-1 were all negative. Positive PCR results found BHV-4 genome from 8 (12.69%) of 63 leukocyte samples. Presence of BVDV and BHV-4 antibodies in unvaccinated animals indicates that these cattle had contracted infection. In conclusion, BHV-4 infections may play a direct or indirect role in causing bovine metritis; therefore their importance in the etiology of metritis and their economic impact needs further attention.},
 year = {2014}
}

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TY  - JOUR
T1  - Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis
AU  - Sibel Yavru
AU  - Oguzhan Avci
AU  - Mehmet Kale
Y1  - 2014/08/30
PY  - 2014
N1  - https://doi.org/10.11648/j.avs.20140205.12
DO  - 10.11648/j.avs.20140205.12
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 142
EP  - 145
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20140205.12
AB  - The purpose of the present study was to evaluate the possible effects of Bovine Herpes virus-1 (BHV-1), Bovine Viral Diarrhea Virus (BVDV), and Bovine Herpes virus-4 (BHV-4) involving metritis in the selected unvaccinated dairy cattle herds in Afyon province of Turkey by serologically and virologically methods. A total of 63 dairy cattle with metritis were sampled in order to investigate the presence of BHV-1, BVDV and BHV-4 infections. The sera samples were tested for presence of antibodies to BHV-1, BVDV and BHV-4 using a commercially available indirect Enzyme Linked Immunosorbent Assay. Leukocyte samples were tested for presence of BVDV viral genome using Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR) for BHV-1 and BHV-4 viral genome. Detectable antibodies were detected in 6 (9.52%) of 63 against BVDV and 51 (80.95%) of 63 against BHV-4. Detectable antibodies were detected against BVDV from 6 (9.52%) of 63 sera samples and BHV-4 from 51 (80.95%) of 63. No antibodies against BHV-1 were detected as well as the results of RT-PCR for BVDV, and PCR for BHV-1 were all negative. Positive PCR results found BHV-4 genome from 8 (12.69%) of 63 leukocyte samples. Presence of BVDV and BHV-4 antibodies in unvaccinated animals indicates that these cattle had contracted infection. In conclusion, BHV-4 infections may play a direct or indirect role in causing bovine metritis; therefore their importance in the etiology of metritis and their economic impact needs further attention.
VL  - 2
IS  - 5
ER  -

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